• 제목/요약/키워드: transport pattern

검색결과 467건 처리시간 0.024초

Glucose Transport through N-Acetylgalactosamine Phosphotransferase System in Escherichia coli C Strain

  • Kim, Hyun Ju;Jeong, Haeyoung;Lee, Sang Jun
    • Journal of Microbiology and Biotechnology
    • /
    • 제32권8호
    • /
    • pp.1047-1053
    • /
    • 2022
  • When ptsG, a glucose-specific phosphotransferase system (PTS) component, is deleted in Escherichia coli, growth can be severely poor because of the lack of efficient glucose transport. We discovered a new PTS transport system that could transport glucose through the growth-coupled experimental evolution of ptsG-deficient E. coli C strain under anaerobic conditions. Genome sequencing revealed mutations in agaR, which encodes a repressor of N-acetylgalactosamine (Aga) PTS expression in evolved progeny strains. RT-qPCR analysis showed that the expression of Aga PTS gene increased because of the loss-of-function of agaR. We confirmed the efficient Aga PTS-mediated glucose uptake by genetic complementation and anaerobic fermentation. We discussed the discovery of new glucose transporter in terms of different genetic backgrounds of E. coli strains, and the relationship between the pattern of mixed-acids fermentation and glucose transport rate.

복합운송인(複合運送人)의 책임(責任) 한계(限界)에 대한 형태별(形態別) 분류(分類)와 실무상(實務上) 적용(適用) (A Pattern of Multimodal Transport Liability and its Adaptation on Practice)

  • 김중관
    • 무역상무연구
    • /
    • 제13권
    • /
    • pp.257-281
    • /
    • 2000
  • The world economy is becoming increasingly globalized. The globalization has resulted in far reaching agreements to deepen trade liberalization and enlarge its scope to cover new areas in addition to strengthening its supporting institutional base. Economic growth has developed international trade which has accelerated the development of international carriage of goods in 21st century. The international trade is basically founded on the contract of international sale of goods and backed up by the contract of international carriage of goods and the insurance on the goods carried. It is essential to incorporate each other sections for the efficient development of international trade. As a result of rapid expansion of international carriage of goods, rationalization of transport was required, which has brought about the International Multimodal Transport System through containerization. The approach to liability system will be a right way to solve the insurance problems for the development and enlargement of world trade volume. International multimodal transport system has affected international trade a lot, especially the field of insurance a grate deal. This paper is to analyze contents of liability system on Multimodal Transport with in the UN Convention on International Multimodal Transport of goods.

  • PDF

The Sediment Transport Pattern from a Large Industrial Complex to an Enclosed Bay

  • Kwon, Young-Tack;Lee, Chan-Won
    • Environmental Engineering Research
    • /
    • 제13권2호
    • /
    • pp.69-72
    • /
    • 2008
  • The movement of sediments in the stream crossing a large industrial complex to the mouth of Masan Bay was monitored for eight years. Sediment samples were seasonally collected in the period of $1992{\sim}1997$ and $2001{\sim}2002$. The heavy metal content of sediment was found to be higher at dry season with the peak on February and significantly decreased at rainy season. Metals content in stream sediments were rapidly decreased by large precipitation events in rainy season because the contaminants in the upstream sediments were transported to the dredged area of Masan Bay where is a typical enclosed bay in Korea. The increasing and decreasing tendency of heavy metals in sediment was repeatedly observed for six consecutive years. The heavy metals assessment of stream sediment provide us the information about the pollutant source, transport pattern and control strategy along the industrial complex. It was strongly suggested that the transportable stream sediments of an industrial area should be controlled as one of the important strategies to restore and manage the enclosed bay. Combined wastewaters have been collected and treated in a publicly owned treatment works (POTW) after industrial wastewater treatment at each location of industries since 1994. A field study was conducted to investigate the pollutant removal efficiency and performance of contact oxidation system installed and operated in two locations in the stream. The stream sediment quality was improved since then, and as a consequence the habitat of the estuary has been restored.

Investigations on aerosols transport over micro- and macro-scale settings of West Africa

  • Emetere, Moses Eterigho
    • Environmental Engineering Research
    • /
    • 제22권1호
    • /
    • pp.75-86
    • /
    • 2017
  • The aerosol content dynamics in a virtual system were investigated. The outcome was extended to monitor the mean concentration diffusion of aerosols in a predefined macro and micro scale. The data set used were wind data set from the automatic weather station; satellite data set from Total Ozone Mapping Spectrometer aerosol index and multi-angle imaging spectroradiometer; ground data set from Aerosol robotic network. The maximum speed of the macro scale (West Africa) was less than 4.4 m/s. This low speed enables the pollutants to acquire maximum range of about 15 km. The heterogeneous nature of aerosols layer in the West African atmosphere creates strange transport pattern caused by multiple refractivity. It is believed that the multiple refractive concepts inhibit aerosol optical depth data retrieval. It was also discovered that the build-up of the purported strange transport pattern with time has enormous potential to influence higher degrees of climatic change in the long term. Even when the African Easterly Jet drives the aerosols layer at about 10 m/s, the interacting layers of aerosols are compelled to mitigate its speed to about 4.2 m/s (macro scale level) and boost its speed to 30 m/s on the micro scale level. Mean concentration diffusion of aerosols was higher in the micro scale than the macro scale level. The minimum aerosol content dynamics for non-decaying, logarithmic decay and exponential decay particulates dispersion is given as 4, 1.4 and 0 respectively.

Modeling of Sediment Transport and Sand Bank Formation in a Macrotidal

  • Park, Moon-Jin
    • Journal of the korean society of oceanography
    • /
    • 제35권1호
    • /
    • pp.1-10
    • /
    • 2000
  • A two-dimensional numerical model was applied to investigate the sediment transport and sand bank formation in a macrotidal sea, the Kyunggi and Asan Bays. The tidal residual currents show quite complex pattern including counter-rotating eddies off the northwestern corner of the Dugjeok Island that reflect the promontory effect. Complex residual eddies are also present off the coast of the Taeanbando and in the Asan Bay. Net sediment transport pattern shows that sandy sediments in the Kyunggi and Asan Bays are generally transported landward from the outer sea suggesting sediment trapping inside the bays. This phenomenon may be related to the formation and maintenance of numerous sand banks in this macrotidal sea. Alternate occurrences of deposition and erosion predicted from the numerical model along the coast of the Taeanbando with strong deposition on the southwestern part of the 'Jangansatoe'(JSB), a large sand ridge off the coast of the Taeanbando appear to reflect the loose connection of JSB, The 'Joongangcheontoe', a central sand bank (CSB) with the main axis in the NW-SE direction in the Asan Bay may undergo a modification with strong deposition along the northeastern flank. These results indicate that the sand banks are actively modified and maintained by the strong tidal currents in this shallow macrotidal sea.

  • PDF

시금치(Spinacia oleracea L.) 엽록체의 광합성 전자전달 활성에 미치는 iso-Butanol의 영향 (Effects of iso-Butanol on Photosynthetic Electron Transport Activity in Isolated Spinach Chloroplasts)

  • 박강은
    • Journal of Plant Biology
    • /
    • 제35권3호
    • /
    • pp.247-252
    • /
    • 1992
  • iso-Butanol이 엽록체의 광합성 전자전달 활성에 미치는 영향을 연구하기 위하여 암조건하에서 시금치의 엽록체에 iso-butanol을 처리한 후 대조구와 비교하여 광계 II 활성과 광계 I 활성을 조사하였으며, 또한 iso-butanol이 틸라코이드 막단백질에 미치는 영향을 알아보기 위해 전기영동으로 틸라코이드 막단백질의 변화를 조사하였다. 광계 I 활성은 iso-butanol이 저농도(1-4%)일 때는 증가하였으나 고농도(5-9%) 에서는 감소되었다. 광계 II 활성은 iso-butanol의 농도가 0.6, 0.8, 1%에서는 각각 75%, 55% 및 25%로 활성이 감소하였다. Butanol 중에서 탄소수는 같으나 OH 기의 위치가 다른 1-butanol, sec-butanol, tert-butanol, iso-butanol이 광계 II의 전자전달계에 미치는 영향을 비교해 본 결과 OH 기를 중심으로 탄소의 배열이 직선상 일 때 저해효과가 크다는 것을 알 수 있었다. iso-butanol이 1차적으로 광계 II에 미치는 억제 부위는 0.8% iso-butanol 처리구에 DPC 첨가했을 때 DCIP 환원율이 93%로 증가하는 것으로 보아 광계 II의 산화 부위임을 알 수 있다. 0.8% iso-butanol 처리구에 외부에서 망간과 칼슘을 첨가하였을 때 광계 II 활성이 각각 83%와 79%로 보호된 것으로 보아 광계 II 활성 억제는 망간의 소실과 관계 있는 것으로 생각된다. 또한 1% iso-butanol 처리한 엽록체를 20배 희석하였을 때 활성이 78%로 회복되었다. iso-Butanol은 1% 이하의 저농도에서 광계 II 활성을 감소시킨데 비해 1% iso-butanol 처리구의 틸라코이드 막단백질의 SDS-PAGE에 의한 band pattern은 대조구와 유사하며 2% 처리구에서는 52 Kd 부근에서 미세한 band pattern의 차이가 있으며 5%의 iso-butanol 처리구의 틸라코이드 막단백질의 band는 전체적으로 많이 소실되었다. 이것으로 보아 iso-butanol은 가역적으로 광계 II의 산화부위에 관여하는 단백질에 영향을 주어 $Ca^{2+}와\;Mn^{2+}$ 이온의 친화력을 저하시켜 광계 II 활성을 억제시키고 또한 알코올의 R-group의 틸라코이드 막에 침투하여 막구조를 변형시키므로 광계 I 활성이 증가하며 고농도(5-9%)에서는 비가역적으로 막구조를 변경하여 광계 I 활성이 감소한 것으로 사료된다.

  • PDF

퇴적물 이동경로 식별을 위한 입도경향 분석법의 가능성과 한계 (Grain-Size Trend Analysis for Identifying Net Sediment Transport Pathways: Potentials and Limitations)

  • 김성환;류호상;유근배
    • 대한지리학회지
    • /
    • 제42권4호
    • /
    • pp.469-487
    • /
    • 2007
  • 입도경향 분석법은 파이척도로 표현된 평균입도, 분급, 왜도 등 퇴적물 입도조직변수가 퇴적물의 이동경로를 따라 특정한 경향을 보이는 성질을 이용한 퇴적물 이동경로 식별 방법론이다. 적용이 간편하고 저렴하여 지형학 연구에 널리 응용될 수 있는 가능성을 지니고 있으나 방법론상의 한계도 몇 가지 측면에서 지적되고 있어 주의가 필요하다. 이 연구는 McLaren과 Bowles의 1차원 경로분석법, Gao와 Collins, Le Roux의 2차원 이동벡터법 등 현재까지 정립된 입도경향 분석의 대표적인 세 가지 기법을 비교 평가하여 적절한 활용법을 도출하고, 입도경향 분석의 추후 연구과제를 제안한 것이다. McLaren-Bowles의 1차원 경로분석법은 연구자의 현장경험을 분석에 효과적으로 결합시킬 수 있고 X-분포를 통해 퇴적환경에 대한 해석을 제공해주며 장기적인 퇴적물 순이동 패턴을 파악하는데 효과적이나 연구자의 주관적 해석에 의존해야 한다는 점, 식별할 수 있는 시간 해상도가 낮다는 점등이 단점이다. Gao-Collins의 2차원 이동벡터법은 명확한 절차, 2차원적인 시각화, 세밀한 시간 해상도 등이 장점이지만, 임계거리 선정, 잡벡터 제거과정 등이 문제를 유발할 수 있으므로 분석 시 주의를 요한다. 셋째, Le Roux의 2차원 이동벡터법은 확장된 경험규칙과 조직변수 간의 구배를 고려하고 시간해상도도 세밀하지만, 분석개념이 모호하고 복잡하다. 입도경향 분석은 현장에 대한 연구자의 이해도, 조사하고자 하는 퇴적물 순이동 패턴의 시간적 스케일, 초점을 맞추고자 하는 정보 등에 따라 적절한 기법을 선택하고, 거기에 부합되는 시료채취방안을 기획하는 것이 중요하다. 또한 입도경향 분석이 지형학 연구에 기여하기 위해서는 시료채취 깊이, 교란층의 두께 등 시료채취 과정의 요소들과 퇴적물 순이동 패턴이 지시하는 시간스케일 간의 관계가 체계적으로 규명되어야 한다고 판단된다.

동해 해수유입에 대한 수치모델: 대마난류 형성 및 수송량 (A Numerical Model on the Inflow into the Japan Sea: the Formation and Transport of the Tsushima Warm Current)

  • 남수용;승영호
    • 한국수산과학회지
    • /
    • 제25권1호
    • /
    • pp.58-64
    • /
    • 1992
  • 북서 태평양에 순압모델을 적용하여 대마난류의 형성과 수송량을 살펴보았다. 모델결과는 대마난류가 대만 부근에서 형성되는 대만난류의 연장임을 보여주고 있다. 계절풍은 대마난류의 수송량 변화에는 그 영향이 미약하지만 대마난류의 유입경로에는 커다란 영향을 미치는 것으로 나타났다. 즉 쿠로시오의 지류가 동지나해로 유입되는 경로가 남풍시는 주로 대만해협으로, 북풍시는 대만 동부를 통해 유입되는 것으로 나타난다.

  • PDF

Bioremediation by Denitrification in the Saturated Zone : Mathematical Model and Experiment

  • Lee Eun-Jung;Lee Kang-Kun;Kim Young;Ha Cheol-Yun
    • 한국지하수토양환경학회:학술대회논문집
    • /
    • 한국지하수토양환경학회 2005년도 총회 및 춘계학술발표회
    • /
    • pp.393-396
    • /
    • 2005
  • The reactive transport model on the biologically mediated sequential nitrate transformation and its subsequent transport was developed and tested. This model was coded as a reaction module within the RT3D framework (Clement, 1997). Transports of the reasonable six mobile solutes (dissolved organic carbon, $O_2,\;{NO_3}^-,\;{NO_2}^-,\;N_2O,\;N_2$) and two immobile microbes were simulated. The simulation results gave a reasonable match with supposed transport pattern. For the next step, the developed model will be validated against experimental data.

  • PDF

새만금 방조제 물막이 구간 주변에서의 지형변화예측(수공) (Time Dependent Morphological Changes around the Closure Gap in Saemankeum)

  • 박영욱;어대수;박상현
    • 한국농공학회:학술대회논문집
    • /
    • 한국농공학회 2000년도 학술발표회 발표논문집
    • /
    • pp.365-370
    • /
    • 2000
  • Sea dike construction for the tidal flat reclamation works in estuary and coast may change the characteristics of tidal motion and wave conditions in the region. In turn, a new hydraulic condition provides the impacts on sediment transport pattern and forms a new morphological environment. Also, morphological changes during the closure works of sea dike are closely related with a safy of sea dike. Therefore, the prediction of morphological changes is required secure the safe closure work and the economic design of sea dikes. To investigate morphological changes due to sea dike construction, hydrodynamic changes of tides and waves have to be evaluated, then sediment transport and sea bottom changes are computed. Mathematical modelling is required for representation of interrelation of tidal motion, wave and sediment transport. In this study, numerical model MORSYS is applied to compute the hydrodynamics and morphological changes around the closure gap for Saemankuem dike. This model allows a flexible integration of the module for waves, currents, sediment transport and bottom changes.

  • PDF