• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.228-233
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• 1998

Seed storage proteins have been used for studying biochemical genetics and end-use quality aspects. We conducted enzyme-linked immunosorbent assay (ELISA) and one-dimensional SDS-PAGE (1D SDS-PAGE) to evaluate different cereal crop species and Korean wheat lines for rye secalin proteins. The antisecalin antibody showed consistent specificity for rye secalin with little cross-reactivity to gliadins. Immunological cross-reactivities measured by the ELISA technique using competition assay showed significant differences of absorbance among rye, triticale, wheat-rye translocated wheat and non-translocated wheat. The absorbance values were lowest in rye followed by triticale, translocated wheat and non-translocated wheat. The ELISA for discrimination of wheat-rye translocation on the basis of antigen-antibody reactivity showed that none of the Korean wheat lines possessed 1RS and secalin proteins. The competitive ELISA experiment demonstrated specific determination for secalin that was originated from rye chromosomal parts. The result of 1D SDS-PAGE for identifying rye secalin subunits showed all three rye specific secalin protein subunits (75 KDa, 45 KDa, and 40 KDa) for rye and triticale, and 1RS specific secalins (45 KDa and 40 KDa) for 1AL/1RS and 1BL/1RS translocated wheats. All Korean wheats were lacking 1RS of rye chromosome and secalin.

• Korean Journal of Environmental Biology
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• v.36 no.1
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• pp.73-81
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• 2018

This study was conducted to understand the current status of the translocated species using a precede study and a model to evaluate the potential invasiveness that could adversely affect the aquatic ecosystem in the Gangneungnamdae Stream. A total of 12 translocated species were investigated and identified from 9 sites in a precede study, and steadily increased since 1982. For the study, which utilized research based on the total FISK (Fish Invasivenss Screening Kit) scores, all of the non-native fishes in Gangneungnamdae Stream were classified into two groups: namely as a high and a medium risk of becoming invasive. It was determined that there were two species (Zacco platypus and Pseudorasbora parva) that were determined to have posed the highest risk. The study determined that the mean scores were shown to have ranged from $3.06({\pm}0.16)-3.42({\pm}0.13)$. Consequently, the habitat analysis showed that the determined QHEI (Qualitative Habitat Evaluation Index) values in the stream averaged 146 (88-171), indicating that an optimal habitat condition did exist in that locale. It can be inferred that compared to land use in the surrounding watersheds, the QHEI values and frequency of translocated species showed the lower the altitude of stream, the QHEI values were decreased and in case of land use pattern, a noted decreased forest and grassland area, and gradually increased urbanized area was seen to exist in the region. The correlation between the fish assemblage, QHEI, land use pattern of surrounding watershed and number of translocated species was identified and analyzed when the stream altitude decreased, and the number of species was increased (r= - 0.782, p=0.0127), the number of species was decreased (r= - 0.737, p=0.0234), and finally when the QHEI values were decreased, it was noted that the urbanized area was increased (r=0.292, p=0.446). In the case of the number of translocated species, when the number of translocated species was increased, the associated urbanized area was increased.

• Korean Journal of Ecology and Environment
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• v.51 no.1
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• pp.71-85
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• 2018

A recent topic of interest within ecology is ecosystem services that are importantly connected with biodiversity. Biodiversity is a fundamental component of ecological studies and has been the subject of many studies worldwide. However, studies on the diversity and distribution of freshwater fish were not undertaken in earnest until 2000, and the number of internationally available studies is therefore still limited. In this study, we used data from "The Survey and Evaluation of Aquatic Ecosystem Health (hereafter SEAEH)" within the framework of which whole water systems on the Korean peninsula are annually monitored to investigate and determine the current status of the diversity and distribution of freshwater fish, as well as to elucidate major factors impacting freshwater fish. A total of 130 species from 28 families were identified at around 953 sites in the SEAEH investigation of 2014. The species Zacco platypus (relative abundance: 28.2%) and Z. koreanus (19.3%) were identified as the most abundant species on the Korean peninsula, and besides these 20 endangered species, 51 endemic species and 4 exotic species were also collected. Highest (96 species) and lowest (72 species) diversity were identified in the Han River Watershed and the Yeongsan/Seomjin River Watershed, respectively. The mean endemic rate was 32.7%, which is higher than the previous rate determined in 1995. The exotic species Micropterus salmoides, Lepomis macrochirus and Carassius cuvieri were found in the whole river system except north Yeongdong, whereas Oreochromis niloticus occurred only at three sites due to the cold water temperatures in winter. Totally, 28 species were found to be translocated to different water systems not connected with known original habitats, most translocations occurring in the Nakdong River and the Yeongdong region. Among the translocated species, Opsariichthys uncirostris amurensis, and Erythroculter erythropterus were dominantly distributed in the mid- to lower reaches of Nakdong River where they may be harmful to the native fish assemblages due to their strong predation. The construction of weirs in connection with the "4 River Project" generated changes in fish assemblages before, during and after the project, resulting in altered habitat condition. Especially, a decrease in the number of endemic species and an increase in the abundance of lentic species were seen. Human-induced global warming will allow exotic species requiring high water temperatures to adapt to the environmental conditions of the Korean peninsula, which may enhance the diversity of native fishes and create changes in distribution. For the establishment of realistic and efficient management and protection strategies for Korean freshwater fish, SEAEH, which produces fundamental and quantitative data for Korean streams, is an important and necessary tool.

• Korean Journal of Ichthyology
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• v.30 no.2
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• pp.119-124
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• 2018

Diversity of fish fauna and species list collected from the Imha-Dam Reservoir of the Nakdonggang River system were reviewed based on the published materials from 1992 to 2016. As a result of the present study, 46 freshwater fish species/subspecies belonging to 36 genera, 11 families, 4 orders were reported in the Imha-Dam Reservoir. Of them, Cyprinidae occupied 58.7% (27 species), Cobitidae 10.9% (5 spp.), Gobiidae 6.5% (3 spp.), and Siluridae, Centropomidae, Centrarchidae were 4.3% (2 spp.) in the number of species, respectively. The dominant species in the number of individuals was Erythroculter erythropterus (40.1%, 9,333 inds.), and the subdominant species was Opsariichthys uncirostris amurensis (9.8%, 2,281 inds.). Among 46 species/subspecies, 17 species (37.0%) were identified as endemic species to Korea. Three species were endangered fish species by the Ministry of Environment of Korea, five translocated species, and three exotic species were reported.

• Korean Journal of Environmental Biology
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• v.33 no.3
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• pp.314-329
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• 2015

The fish fauna was investigated from 2010 and 2013 at main stream and tributaries in the Seomjin River which has no estuary dike. The collected fishes were identified into 37,751 individuals of 107 species of 81 genera belonging to 41 families. Among the collected fishes the primary freshwater fishes were 57 species (Relative Abundance: 53.3%), the secondary fishes were 10 species (RA: 9.3%) and the marine fishes were 40 species (RA: 37.4%). The dominant species was Zacco platypus (RA: 28.7%), and the subdominant species was Zacco koreanus (RA: 15.0%). The endemic species were 21 species (RA: 31.3%). The endangered fishes were Acheilognathus somjinensis (Level I), Lampetra reissneri (Level II) and Microphysogobio koreensis (Level II). The exotic species were Carassius cuvieri, Lepomis macrochirus and Micropterus salmoides. We supposed that Odontobutis interrupta in the Seomjin River is translocated species.

• Journal of Plant Biology
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• v.37 no.3
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• pp.293-299
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• 1994

Developing rice endosperm cells display two morphologically distinct rough endoplasmic reticulum (ER) membranes, the cisternae ER (C-ER) and theprotein body ER (PB-ER), the latter delimiting the prolamine protein bodies. We (Li et al., 1993) have recently shown that the storage protein mRNAs are not randomly distributed on these ER types; the C-ER is enriched for glutelin mRNAs, whereas the PB-ER harbors predominantly prolamine transcripts. To address whether these ER types have differnet capacities to translate these mRNAs and translocate their proteins into the lumen, a microsomal fraction enriched in C-ER vesicles was prepared from devleoping rice seeds. When present in an in vitro translatin system, the microsomes were able to proteolytically remove the signal peptide and internalize both preproglutelin and preprolamine within the microsomal vesicles. Of the two species, preprolamine was more effectively translocated and processed. These results suggest that the C-ER has the capacity to recognize and bind both storage protein mRNAs during protein synthesis. Moreover, efficient translocation and processing of glutelin requires additional factors that are deficient or absent in the in vitro system.

• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.19-28
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• 1999

To investigate the physiological and biochemical differences between susceptible and tolerant species of Echinochloa species to the foliar applied cyhalofop-butyl and pyribenzoxim herbicides, herbicidal response, the rate of absorption, translocation and metabolism were studied. Among 148 individuals of Echinochloa species collected from 41 sampling sites in Korea which were classified into 7 geographical regions, based on morphological characteristics of plant and seed type, 46 lines were used for further studies. From them, E. crus-galli var. praticola collected from Hwanggan and E. crus-galli var. crus-galli collected from Namyangju were selected as the most susceptible species to cyhalofop-butyl and pyribenzoxim, respectively. Meanwhile, E. oryzicola(from Cheju) and E. crus-galli var. crus-galli(from Asan) were selected as tolerant species to cyhalofop-butyl and pyribenzoxim, respectively. Application of radio-labelled herbicides on the 1st leaf to both susceptible and tolerant Echinochloa species exhibited that the applied $^{14}C$-Cyhalofop-butyl and $^{14}C$-pyribenzoxim were more easily absorbed and translocated into stem tissues than root. Absorption of $^{14}C$-Cyhalofop-butyl increased rapidly at 1 h after application and reached the maximum at 12 h after application. However, the absorption rate of $^{14}C$-pyribenzoxim was not changed. Two metabolites from cyhalofop-butyl-treated plants and one metabolite from pyribenzoxim-treated plants were separated by TLC. The amount of metabolite 1 in cyhalofop-butyl-treated tolerant species was significantly higher than that in the susceptible one, suggesting differences in detoxification ability between susceptible and tolerant species.

• Korean Journal of Ichthyology
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• v.31 no.2
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• pp.90-100
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• 2019

This study was carried out from March to November, 2013 to investigate the fish community structure and distribution characteristics of Phoxinus phoxinus and Rhynchocypris kumgangensis appearing in Gihwacheon Stream, Pyeongchang. A total of 6 families and 19 species of fish emerged during the survey period. The dominant species were R. kumgangensis (Individuals 44.7%, Biomass 30.2%) and P. phoxinus (Individuals 27.7%, Biomass 15.9%). Endemic species were 10 species including Pseudopungtungia tenuicorpus, Coreoleuciscus splendidus, Hemibarbus mylodon, Gobiobotia brevibarba, Microphysogobio longidorsalis, R. kumgangensis, Zacco koreanus, Korecobitis rotundicaudata, Iksookimis koreensis and Coreoperca herzi. Endogenous species were P. tenuicorpus, G. brevibarba and P. phoxinus. Hemibarbus mylodon was a natural monument. Oncherhynchus mykiss was exotic species and translocated species was Oncorhynchus masou masou. The dominant species, R. kumgangensis, appeared at all survey sites. Except for St.6, the b value was more than 3.0 and the slope of K value showed positive correlation. P. phoxinus appeared in St. 3~St. 6 and St. 3 and St. 4 only showed stable populations.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.5 no.2
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• pp.141-149
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• 2004

The aim of this study was to test the validity of the hypothesis that the karyotypes of four species of Lepilemuridae were formed spontaneously from their ancestral hybrid karyotype. Hypothetical ancestral haploid Karyotype of Lepilemuridae is composed of 18 autosomes and X chromosomes. Lepilemur mustelinus karyotype has four tandem fused chromosomes and one Robertsonian translocated chromosome pairs. Lepilemur septentrionalis septentrionalis karyotype has only two pairs of translocated chromosomes. We reconstruct and suggest ancestral karyotype of LMU(ancLMU) and LSS(ancLSS), from which all four studied species were derived. Hybrids of ancLMU and ancLSS were formed and produce differently fused equilibrated gametes via circular form arrangement during gametogenesis. Five unit of trivalent homologous chromosome pairs were engaged in a circular form to give new gamete corresponding to the karyotype of L. dorsalis, orientation of one unit of trivalent was inversed in the circle to gave new gamete corresponding to the karyotype of L. leucopus. Seven homologous chromosome pairs were engaged in circular form to give haploid karyotype of Lepilemur ruficaudatus. Only one homologous chromosome pair is dissociated and the other chromosome pairs rearranged in the circle to form haploid karyotype of Lepilemur edwardsi. The new gametes could be produced from these circular forms. When the new gamete fertilized with the same type of gamete, The new homozygote is produced as existing L. dorsalis, L. leucopus, L. edwardsi and L. ruficaudatus. These results support the theory that new species could be formed in hybrid population through activated chromosome fusion, chromosome rearrangement in circular from at zygotene stage and production of equilibrated gametes to form homozygote new species.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.268-275
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• 2006

ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide by the action of the protoporphyrinogen IX oxidase (Protox IX). A study was conducted to determine photodynamic herbicidal effect of ALA on seedling growth of rice (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) under dry and wet conditions. ALA effect on early plant growth of rice and barnyardgrass was greatly concentration dependant, suggesting that it promotes plant growth at very low concentration and inhibits at high concentration. No significant difference in herbicidal activity of biologically and synthetically produced ALAs on plant lengths of test plants was observed ALA exhibited significant photodynamic activity regardless of PSDIP and its duration. Significant shoot growth inhibition by ALA soaking treatment exhibited apparently, indicating that ALA absorbed through root system was translocated into shoot part of plants. ALA reduced plant heights of rice and barnyardgrass seedlings by 6% and 27%, respectively, showing more tolerant to ALA in rice under wet condition. Leaf thickness was reduced markedly by ALA with increasing of ALA concentration, due to mainly membrane destruction and severe loss of turgidity in mesophyll cells, although the epidermal was little affected. It was observed that photodynamic herbicidal activity of ALA applied by pre-and post-emergence application exhibited differently on plant species, and that the activity of ALA against susceptible plants was highly correlated with growing condition.