• 대한기계학회논문집A
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• 제31권12호
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• pp.1165-1172
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• 2007

The spacer grid set is a part of a nuclear fuel assembly. The set has a spring and the spring supports the fuel rods safely. Although material nonlinearity is involved in the deformation of the spring, nonlinearity has not been considered in design of the spring. Recently a nonlinear response structural optimization method has been developed using equivalent loads. It is called nonlinear response optimization equivalent loads (NROEL). In NROEL, the external loads are transformed to the equivalent loads (EL) for linear static analysis and linear response optimization is carried out based on the EL in a cyclic manner until the convergence criteria are satisfied. EL is the load set which generates the same response field of linear analysis as that of nonlinear analysis. Shape optimization of the spring is carried out based on EL. The objective function is defined by minimizing the maximum stress in the spring while mass is limited and the support force of the spring is larger than a certain value. The results are verified by nonlinear response analysis. ABAQUS is used for nonlinear response analysis and GENESIS is employed for linear response optimization.

• 대한기계학회논문집A
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• 제31권11호
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• pp.1059-1068
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• 2007

Nonlinear response structural optimization is performed using equivalent loads (NROEL). Nonlinear response optimization is extremely cost because many nonlinear analyses are required. In NROEL, the external loads are transformed to the equivalent loads (EL) for linear static analysis and linear response optimization is carried out based on the EL in a cyclic manner until the convergence criteria are satisfied. EL is the load set which generates the same response field of linear analysis as that of nonlinear analysis. The primitive from of theory has been published. In this research, the theory is investigated with large scale example problems. Four examples are solved by using NROEL. Conventional optimization with sensitivity analysis using the finite difference method (FDM) is also applied to the same examples. Moreover, response surface optimization method is applied to the last two examples. The results of the optimizations are compared. In nonlinear response optimization of large scale problems, hundreds (or even thousands) of nonlinear analyses are expected to satisfy the convergence criteria. However, in nonlinear response optimization using equivalent loads, only tens of nonlinear analyses are required. The results are discussed and the usefulness of NROEL is presented.

• Journal of Plant Biotechnology
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• 제40권1호
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• pp.49-54
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• 2013

C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.

• Ocean Systems Engineering
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• 제6권4호
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• pp.363-375
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• 2016

Large numbers of submarine pipelines are laid as the world now is attaching great importance to offshore oil exploitation. Free spanning of submarine pipelines may be caused by seabed unevenness, change of topology, artificial supports, etc. By combining Iwan's wake oscillator model with the differential equation which describes the vibration behavior of free-span submarine pipelines, the pipe-fluid coupling equation is developed and solved in order to study the effect of both internal and external fluid on the vibration behavior of free-span submarine pipelines. Through generalized integral transform technique (GITT), the governing equation describing the transverse displacement is transformed into a system of second-order ordinary differential equations (ODEs) in temporal variable, eliminating the spatial variable. The MATHEMATICA built-in function NDSolve is then used to numerically solve the transformed ODE system. The good convergence of the eigenfunction expansions proved that this method is applicable for predicting the dynamic response of free-span pipelines subjected to both internal flow and external current.

• 대한기계학회논문집A
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• 제27권2호
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• pp.268-275
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• 2003

Generally, structural optimization is carried out based on external static loads. All forces have dynamic characteristics in the real world. Mathematical optimization with dynamic loads is extremely difficult in a large-scale problem due to the behaviors in the time domain. The dynamic loads are often transformed into static loads by dynamic factors, design codes, and etc. Therefore, the optimization results can give inaccurate solutions. Recently, a systematic transformation has been proposed as an engineering algorithm. Equivalent static loads are made to generate the same displacement field as the one from dynamic loads at each time step of dynamic analysis. Thus, many load cases are used as the multiple leading conditions which are not costly to include in modern structural optimization. In this research, it is mathematically proved that the solution of the algorithm satisfies the Karush-Kuhn-Tucker necessary condition. At first, the solution of the new algorithm is mathematically obtained. Using the termination criteria, it is proved that the solution satisfies the Karush-Kuhn-Tucker necessary condition of the original dynamic response optimization problem. The application of the algorithm is discussed.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2000년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2000
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• pp.66-72
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• 2000

We have scanned the several seismic traces of earthquakes and blasts observed from the digital new type seismograph instruments of KMA from Jan. 2000 to Aug. 2000. From these data, good quality data which have high signal/noise ratio were selected and they were transformed into ascii data from binary data(mini-seed format). The hypo71 program and P-S was applied in order to determine the location of epicenter, origin time and the magnitude. From these data, the 18 earthquakes and 3 blasts, 207 seismic records consist of 359 directional components were calculated. Using theses ground acceleration data, acceleration, velocity, and displacement response spectrums of the structures were calculated and they could be represented in a picture by the form of tripartite response spectrum. In the result, response spectrums of the 359 directional components of the above seismic data records were obtained respectively.

• Interaction and multiscale mechanics
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• 제2권3호
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• pp.263-276
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• 2009

This paper is intended to investigate interaction response of a train running over a suspension bridge undergoing support settlements. The suspension bridge is modeled as a single-span suspended beam with hinged ends and the train as successive moving oscillators with identical properties. To conduct this dynamic problem with non-homogeneous boundary conditions, this study first divides the total response of the suspended beam into two parts: the static and dynamic responses. Then, the coupled equations of motion for the suspended beam carrying multiple moving oscillators are transformed into a set of nonlinearly coupled generalized equations by Galerkin's method, and solved using the Newmark method with an incremental-iterative procedure including the three phases: predictor, corrector, and equilibrium-checking. Numerical investigations demonstrate that the present iterative technique is available in dealing with the dynamic interaction problem of vehicle/bridge coupling system and that the differential movements of bridge supports will significantly affect the dynamic response of the running vehicles but insignificant influence on the bridge response.

• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.955-963
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• 2023

Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis-acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.

• Geomechanics and Engineering
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• 제15권3호
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• pp.919-925
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• 2018

Stress-strain responses of weak-to-strong carbonate rocks used for tunnel construction were studied. The analysis of applicability of exponential stress-strain models based on Haldane's distribution function is presented. It is revealed that these exponential equations presented in transformed forms allow us to predict stress-strain relationships over the whole pre-failure strain range without mechanical testing of rock samples under compression using a press machine and to avoid measurements of axial failure strains for which relatively large values of compressive stress are required. In this study, only one point measurement (small strain at small stress) using indentation test and uniaxial compressive strength determined by a standard Schmidt hammer are considered as input parameters to predict stress-strain response from zero strain/zero stress up to failure. Observations show good predictive capabilities of transformed stress-stress models for weak-to-strong (${\sigma}_c$ <100 MPa) heterogeneous carbonate rocks exhibiting small (< 0.5 %), intermediate (< 1 %) and large (> 1 %) axial strains.

• 한국작물학회지
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• 제47권2호
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• pp.116-122
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• 2002

Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK$_3$ LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.