• 한국항만학회지
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• 제12권1호
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• pp.75-86
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• 1998

To design a coastal structure in the nearshore region, engineers must have means to estimate wave climate. Waves, approaching the surf zone from offshore, experience changes caused by combined effects of bathymetric variations, interference of man-made structure, and nonlinear interactions among wave trains. This paper has attempted to find out the effects of two of the more subtle phenomena involving nonlinear shallow water waves, amplitude dispersion and secondary wave generation. Boussinesq-type equations can be used to model the nonlinear transformation of surface waves in shallow water due to effect of shoaling, refraction, diffraction, and reflection. In this paper, generalized Boussinesq equations under the complex bottom condition is derived using the depth averaged velocity with the series expansion of the velocity potential as a product of powers of the depth of flow. A time stepping finite difference method is used to solve the derived equation. Numerical results are compared to hydraulic model results. The result with the non-linear dispersive wave equation can describe an interesting transformation a sinusoidal wave to one with a cnoidal aspect of a rapid degradation into modulated high frequency waves and transient secondary waves in an intermediate region. The amplitude dispersion of the primary wave crest results in a convex wave front after passing through the shoal and the secondary waves generated by the shoal diffracted in a radial manner into surrounding waters.

• Molecules and Cells
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• 제37권4호
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• pp.330-336
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• 2014

Thymosin beta4 (TB4) has multiple functions in cellular response in processes as diverse as embryonic organ development and the pathogeneses of disease, especially those associated with cardiac coronary vessels. However, the specific roles played by TB4 during heart valve development in vertebrates are largely unknown. Here, we identified a novel function of TB4 in endothelial-mesenchymal transformation (EMT) in cardiac valve endocardial cushions in zebrafish. The expressions of thymosin family members in developing zebrafish embryos were determined by whole mount in situ hybridization. Of the thymosin family members only zTB4 was expressed in the developing heart region. Cardiac valve development at 48 h post fertilization was defected in zebrafish TB4 (zTB4) morpholino-injected embryos (morphants). In zTB4 morphants, abnormal linear heart tube development was observed. The expressions of bone morphogenetic protein (BMP) 4, notch1b, and hyaluronic acid synthase (HAS) 2 genes were also markedly reduced in atrio-ventricular canal (AVC). Endocardial cells in the AVC region were stained with anti-Zn5 antibody reactive against Dm-grasp (an EMT marker) to observe EMT in developing cardiac valves in zTB4 morphants. EMT marker expression in valve endothelial cells was confirmed after transfection with TB4 siRNA in the presence of transforming growth factor ${\beta}$ ($TGF{\beta}$) by RT-PCR and immunofluorescent assay. Zn5-positive endocardial AVC cells were not observed in zTB4 morphants, and knockdown of TB4 suppressed TGF-${\beta}$-induced EMT in ovine valve endothelial cells. Taken together, our results demonstrate that TB4 plays a pivotal role in cardiac valve formation by increasing EMT.

• 한국정보과학회논문지:데이타베이스
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• 제31권4호
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• pp.362-372
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• 2004

분산 지리정보시스템을 구현하는데 가장 어려운 점 중의 하나는 공간데이타베이스의 불일치이다. 특히, 여러 가지 이유로 발생하는 분산 공간 데이타베이스 사이의 위치 불일치는 공간 질의의 결과를 부정확하게 만든다. 하나의 간단한 해결책은 가장 중요한 사이트에 따라 각 사이트의 공간데이타베이스에 있는 위치 데이타를 보정하는 것이다. 그러나, 이러한 방법은 각 사이트의 자율성이 중시되어야 하는 경우에는 실용적이지 않다. 본 논문에서는 위치 데이타가 불일치하는 여러 개의 공간데이타베이스에 대하여 공간질의를 처리하는 방법을 제시한다. 본 논문에서 제시하는 방법은 각 지역 공간데이타베이스를 수정하지 않고 자율성을 보장하는 방식으로 공간질의를 처리할 수 있도록 한다. 본 논문에서 제안하는 방법은 탄성변형방법을 이용하면서, 각 질의의 위치를 동적으로 변환하는 방법에 기초하고 있다. 이 방법의 정확성은 수학적으로 증명이 되었으며, 실험으로도 확인하였다. 더욱이, 이 방법의 유용성 검증을 위하여 상용 데이타베이스시스템을 이용하여 구현하였다.

• Journal of Microbiology
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• 제37권4호
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• pp.256-262
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• 1999

Latent membrane protein 1 (LMP1) of the Epstein-Barr virus (EBV) is an integral membrane protein with six transmembrane domains, which is essential for EBV-induced B cell transformation. LMP1 functions as a constitutively active tumor necrosis factor receptor (TNFR) like membrane receptor, whose signaling requires recruitment of TNFR-associated factors (TRAFs) and leads to NF-${\kappa}B$ activation. NF-${\kappa}B$ activation by LMP1 is critical for B cell transformation and has been linked to many phenotypic changes associated with EBV-induced B cell transformation. Deletion analysis has identified two NF-${\kappa}B$ activation regions in the carboxy terminal cytoplasmic domains of LMP1, termed CTAR1 (residues 194-232) and CTAR2 (351-386). The membrane proximal C-terminal domain was precisely mapped to a PXQXT motif (residues 204-208) involved in TRAF binding as well as NF-${\kappa}B$ activation. In this study, we dissected the CTAR2 region, which is the major NF-${\kappa}B$ signaling effector of LMP1, to determine a minimal functional sequence. A series of LMP1 mutant constructs systematically deleted for the CTAR2 region were prepared, and NF-${\kappa}B$ activation activity of these mutants were assessed by transiently expressing them in 293 cells and Jurkat T cells. The NF-${\kappa}B$ activation domain of CTAR2 appears to reside in a stretch of 6 amino acids (residues 379-384) at the end of the carboxy terminus.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권3호
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• pp.233-240
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• 2011

Purpose: To apply a computer assisted navigation system to orthognathic surgery, a simple and efficient measuring algorithm calculation based on affine transformation was designed. A method of improving accuracy and reducing errors in orthognathic surgery by use of an optical tracking camera was studied. Methods: A total of 5 points on one surgical splint were measured and tracked by the Polaris $Vicra^{(R)}$ (Northern Digital Inc Co., Ontario, Canada) optical tracking system in two cases. The first case was to apply the transformation matrix at pre- and postoperative situations, and the second case was to apply an affine transformation only after the postoperative situation. In each situation, the predictive measuring value was changed to the final measuring value via an affine transformation algorithm and the expected coordinates calculated from the model were compared with those of the patient in the operation room. Results: The mean measuring error was $1.027{\pm}0.587$ using the affine transformation at pre- and postoperative situations and the average value after the postoperative situation was $0.928{\pm}0.549$. The farther a coordinate region was from the reference coordinates which constitutes the transform matrixes, the bigger the measuring error was found which was calculated from an affine transformation algorithm. Conclusion: Most difference errors were brought from mainly measuring process and lack of reproducibility, the affine transformation algorithm formula from postoperative measuring values by using of optic tracking system between those of model surgery and those of patient surgery can be selected as minimizing the difference error. To reduce coordinate calculation errors, minimum transformation matrices must be used and reference points which determine an affine transformation must be close to the area where coordinates are measured and calculated, as well as the reference points need to be scattered.

• 미생물학회지
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• 제41권4호
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• pp.239-245
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• 2005

Transformation-associated recombination (TAR) 클로닝 법은 복잡한 게놈으로부터 염색체 내의 특정부위나 유전자를 선택적으로 분리할 수 있다. 이 방법은 목적 유전자에 근접한 작은 게놈DNA 염기서열 정보를 필요로 한다. 이 기술은 효모의 spheroplast transformation을 시키는 동안 목적으로 하는 유전자의 5' 또는 3' 서열을 포함하고 있는 TAR vector와 게놈DNA사이에서 일어나는 상동성재조합에 의해 이루어진다. 본 연구에서는 plasmid 모델시스템을 이용하여 target hooks 내에 존재하는 비상동성 염기서 열이 상동성재조합에 미치는 영향을 조사하였다. plasmid에 존재하는HIS3유전자와 변형시킨 his3-TRP1-his3 단편 사이의 상동성재조합의 효율은 $Ura^+$ 형질전환체의 형질분석에 의해 이루어졌다. $Ura^+$ 형질전환체의 수는 7종류의 서로 달리 변형된 his3-TRP1-his3 단편들을 사용하였을 매 거의 동일하게 나타났다. 그러나 $Trp^+His^+$ positive recombinants의 빈도는 변형된 his3-TRP1-his3 단편 내에 비상동성 영역에 부정확한 간격을 지닐 때 현저한 감소를 나타내었다. 이러한 결과로서, 부정확한 간격이 target hook과 substrate DNA 사이에 일어나는 상동성재조합을 방해하는 것으로 사료된다. 그러므로 이종간의 상동유전자를 클로닝 할 때에는 target hook내의 비상동성 염기서열이 존재한다면 이것이 정확한 간격을 지니는지 여부를 중요란 요인으로 고려해야 한다.

• 한국농공학회지
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• 제18권1호
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• pp.4071-4078
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• 1976

The authors analyzed the seepage by means of the following mathmatical solutions of the Laplace Equations on the given boundary conditions. The boundaries of the flow region are of two types i) impervious boundaries (${\Phi}$=constant), and ii) reservoir boundaries (${\Phi}$=constant). The corresponding w plane, bounding the flow region, is the rectangle in Fig. 8-a. As the z plane and w plane are both polygons, by means of the Schwarz-Christoffel transformation the flow region in each of these planes can be mapped con for mally onto the same half of an auxiliary t plane, there by yielding, say, the functions z=f1(t) and w=f2(t). Then, either by eliminating the variable t or by using t as a parameter, the function w=f(z) can be established.

• 한국해양공학회지
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• 제7권1호
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• pp.81-91
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• 1993

Studies on the flow pattern due to reclamation in the Myunggi-Nocksan region are carried out based on field observations and numerical experiments. Serial time series measurement of current, temperature and salinity were conducted for 1 tidal period at intervals of 1 hour from surface to bottom on station located at 128.deg. 54' 44" E, 35.deg. 01' 04" N in April and June 1992. Surface current opposite to the subsurface current causes turbulent mixing to make homogeneous salinity pattern in vertical section. Reclamation has little effect on the wave pattern and flow patterns are also nearly similar except on the nearby region of reclamation where flow speed somewhat weakened.

• 한국재료학회지
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• 제23권6호
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• pp.329-333
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• 2013

The structural phase transformations of $0.7Pb(Mg_{1/3}Nb_{2/3})O_3-0.3PbTiO_3$ (PMN-0.3PT) were studied using high resolution x-ray diffraction (HRXRD) as a function of temperature and electric field. A phase transformational sequence of cubic (C)${\rightarrow}$tetragonal (T)${\rightarrow}$rhombohedral (R) phase was observed in zero-field-cooled conditions; and a $C{\rightarrow}T{\rightarrow}$monoclinic $(M_C){\rightarrow}$ monoclinic ($M_A$) phase was observed in the field-cooled conditions. The transformation of T to $M_A$ phase was realized through an intermediate $M_C$ phase. The results also represent conclusive and direct evidence of a $M_C$ to $M_A$ phase transformation in field-cooled conditions. Beginning from the zero-field-cooled condition, a $R{\rightarrow}M_A{\rightarrow}M_C{\rightarrow}T$ phase transformational sequence was found with an increasing electric field at a fixed temperature. Upon removal of the field, the $M_A$ phase was stable at room temperature. With increasing the field, the transformation temperature from T to $M_C$ and from $M_C$ to $M_A$ phase decreased, and the phase stability ranges of both T and $M_C$ phases increased. Upon removal of the field, the phase transformation from R to $M_A$ phase was irreversible, but from $M_A$ to $M_C$ was reversible, which means that $M_A$ is the dominant phase under the electric field. In the M phase region, the results confirmed that lattice parameters and tilt angles were weakly temperature dependent over the range of investigated temperatures.

• 분석과 대안
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• 제2권2호
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• pp.93-110
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• 2018

Due to its speciality, Johannesburg has drawn wide attention from the international academia, which the existing urban theory cannot easily explain. This paper focuses on the spatial pattern of Johannesburg, and finds that it is highly fragmented: first North-South Division due to apartheid and gold mining, secondly, the continuous sprawl due to population densification, and thirdly formation of city-region due to the fill-in spatial policy. The fragmented spatial pattern undermines the social integration and weakens the potential economic sustainability, which make the balanced multi-core spatial pattern inevitable under the influence of the international planning thought. The Johannesburg's spatial transformation comes not only from social integration, but also from the development vison of world-class status of an African city. In reality, since the collapse of apartheid, the municipal government has released series of strategic planning in different executive phase. These different plannings unanimously focus on the world class status although since 2008, the social inclusiveness is more emphasized. Thus, Johannesburg implements some spatial policies, as promoting the corridor of freedom, identifying the key nodes in the city then developing with high density, implementing the in-filling policy, managing the urban growth boundary, effectively preserving the natural system. However, the industrial structure in Johannesburg is already quite high-ended which cannot produce many jobs and also require much investment in advanced infrastructure. Thus, the strategic goals of world-class status of an African city and social inclusiveness which really need widely shared public services to some extent are in tension. After evaluation, we can see that spatial transformation is quite limited. Obviously spatial transformation largely depends on the social-economy. The population is still in flow. In this sense Johannesburg must energetically support the employment-based industry, effectively control the spatial sprawl and carry out institutional innovation which further incentive investment, gradually build an integrated regional governance. In general, how Johannesburg combines globalization and its own condition is still worth thinking in both theory and practice.