• Journal of Plant Biology
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• 제39권2호
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• pp.93-98
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• 1996

In order to enhance the system of potato transformation and further regeneration, potato was transformed using the Agrobacterium tumefaciens harboring $\beta$-glucuronidase (GUS) gene. We found that a series fo modified medium ttained 100% shoot regeneration within 5 weeks after the preincubated explants on stage I medium were infected with Agrobacterium. Callus appeared at the cut edges of stem segments on stage II medium, mainly at the basal parts. Some explants started to form shoots after two to three weeks on stage III medium containing kanamycin (50 mg/L). When transferred to MS medium containing 200 mg/L kanamycin, 81% of the transformed shoots formed roots at the cut edge of the plantlets. In contrast, untrasformed shoots never rooted and became yellowish after few weeks under the same conditions. Southern and northern analysis indicated in vitro shoot regeneration on the callus derived from the potato explants, which were incubated with Agrobacteria. The regeneration cycle was shortened after the transformatin and finally the transformation efficiency was highly enhanced.

• KSBB Journal
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• 제21권6호
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• pp.489-492
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• 2006

분자육종의 목적을 위하여 Agrobacterium을 이용한 Brassica napus 식물의 유전적 형질전환은 폭 넓게 시행되어 왔다. B. napus cv. napus는 유지작물의 하나이면서 이 또한 Agrobacterium을 이용한 형질전환이 가능하다. 본 연구에서는 agroinfiltration방법을 이용시 유채유묘의 형질전환이 낯은 효율로 나타나고 있으며 이는 fluorometric GUS assay에 의하여 판단되었다. 대조적으로 유채유묘에 대하여 sodium hydrosulfite 용액을 agroinfiltration 과정 이전에 처리할 경우 형질전환율이 상당히 증가하는 것을 관찰할 수 있었다. RT-PCR에 의한 GUS유전자발현의 확인을 통하여 유채유도를 이용한 일시발현체계의 개발가능성을 제시하였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.209-212
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• 2002

Since Berkhoff proposed the mild-slope equation in 1972, it has widely been used for calculation of shallow water wave transformation. Recently, it was extended to give an extended mild-slope equation, which includes the bottom slope squared term and bottom curvature term so as to be capable of modeling wave transformation on rapidly varying topography. These equations were derived by integrating the Laplace equation vertically. In the present study, we develop a finite element model to solve the Laplace equation directly while keeping the same computational efficiency as the mild-slope equation. This model assumes the vertical variation of wave potential as a cosine hyperbolic function as done in the derivation of the mild-slope equation, and the Galerkin method is used to discretize . The computational domain was discretized with proper finite elements, while the radiation condition at infinity was treated by introducing the concept of an infinite element. The upper boundary condition can be either free surface or a solid structure. The applicability of the developed model was verified through example analyses of two-dimensional wave reflection and transmission. .

• 대한기계학회논문집A
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• 제31권3호
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• pp.331-337
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• 2007

Rotating cantilever beams can be found in several practical engineering applications such as turbine blades and helicopter rotor blades. For reliable and economic design, it is necessary to estimate the dynamic characteristics of those structures accurately and efficiently since significant variation of dynamic characteristics resulted from rotational motion of the structures. Recently, Differential Transformation Method(DTM) was proposed by Zhou. This method has been applied to fluid dynamics and vibration problems, and has shown accuracy, efficiency and convenience in solving differential equations. The purpose of this study, the free vibration analysis of a rotating cantilever beam, is to seek for the reliable property of DTM and confidence in the results obtained by this method by comparing the results with that of finite element method applied to linear partial differential equations. In particular, this study is worked by supposing optional T-function values because the equations governing chordwise motion are based on two differential equations coupled with each other. This study also shows mode shapes of rotating cantilever beams for various rotating speeds.

• ETRI Journal
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• 제38권5호
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• pp.807-817
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• 2016

A novel fast algorithm is suggested for a coding unit (CU) mode decision using pseudo rate-distortion based on a separated encoding structure in High Efficiency Video Coding (HEVC). A conventional HEVC encoder requires a large computational time for a CU mode prediction because prediction and transformation procedures are applied to obtain a rate-distortion cost. Hence, for the practical application of HEVC encoding, it is necessary to significantly reduce the computational time of CU mode prediction. As described in this paper, under the proposed separated encoder structure, it is possible to decide the CU prediction mode without a full processing of the prediction and transformation to obtain a rate-distortion cost based on a suitable condition. Furthermore, to construct a suitable condition to improve the encoding speed, we employ a pseudo rate-distortion estimation based on a Hadamard transformation and a simple quantization. The experimental results show that the proposed method achieves a 38.68% reduction in the total encoding time with a similar coding performance to that of the HEVC reference model.

• 대한기계학회논문집
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• 제16권5호
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• pp.883-890
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• 1992

본 연구에서는 Laplace 변환법에 의한 2차원 동적 문제의 경계요소 프로그램 을 작성하고, 간단한 모델을 해석하여 프로르램의 정도 및 그 유용성을 검토하고, 응 용문제로 동적하중을 받는 균열문제의 몇 가지 모델에 대하여 변위 외삽법으로 균열의 파괴 역학적 파라미터인 동적응력확대계수(dynamic stress intensity factor)를 구하 여 결과를 검토하여 보고자 한다.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.414-424
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• 2010

This report describes recent advances in tissue culture, genetic transformation of commercial rose (Rosa hybrida) and in development of new rose cultivars by molecular breeding. Rose is one of major cut-flowers in global horticulture industry. Successful progresses were made in development of new cultivars for pathogen resistant, environmental stress resistant and petal color modification by molecular breeding. New cultivars, however, has not reported yet in korea, although lots of progresses were achieved in each field of conventional breeding, tissue culture and genetic transformation. Cooperation in these research fields will promote screening of useful genes to have specific traits on rose and exploiting of processes to improve in the efficiency of tissue culture and genetic transformation of rose, therefore, we hopefully expect that new rose cultivars by molecular breeding will be released in the near future.

• 열처리공학회지
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• 제16권4호
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• pp.205-210
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• 2003

Various types of high strength steel sheets were usually used for improving the automobile safety and fuel efficiency by reducing the vehicle weight. The present study aimed to develop the TRIP (transformation induced plasticity) aided high-strength low carbon steel sheets by using a reverse transformation process. The 0.1C-4~8Mn steels were reverse-transformed by slow heating to intercritical temperature region and then furnace cooled to the room temperature. Granular type retained austenite was observed in 4Mn steel and lath type retained austenite was also observed in 6~8Mn steel. The results show that the 6Mn steel under reverse transformed at $625^{\circ}C$ for 6 hrs has maximum elongation up to 39%. The optimum strength-elongation combination was 3,888 ($kg/mm^2{\times}%$) when the 8Mn steel was reverse transformed at $625^{\circ}C$ for 12 h.

• 생명과학회지
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• 제15권6호
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• pp.1034-1036
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• 2005

배추 종자 및 유묘에 대하여 GUS발현 또는 hepatitis B surface antigen (HBsAg)발현 벡터를 지니는 Agrobacterium tumefaciens LBA4404 세포를 이용하여 진공침윤(agroinfiltration)에 의한 형질전환을 시도하였다. 특히 ELISA를 이용한 HBsAg발현의 정량적 분석에서 agroinfiltration방법은 형질전환효율이 매우 저조하게 나타났다. 그러나 차아염소산나트륨 용액을 발아 전 또는 발아 중인 배추종자에 처리한 후 agroinfiltration을 실시한 경우 형질전환 효율이 $2\~5$배 증가하였다. 따라서 차아염소산나트륨 등의 화학연마제에 의한 종자의 상처발생이 Agrobacterium의 감염을 용이하게 함으로써 배추유묘에서의 일시유전자발현을 증대시키는 것으로 제안되고있다.

• Journal of Plant Biotechnology
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• 제48권2호
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• pp.93-99
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• 2021

This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. "Jinju" to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 μM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3-butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.