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http://dx.doi.org/10.5352/JLS.2005.15.6.1034

Sodium Hypochlorite Solution As a Chemical Wounding Agent for Improving Agrobacterium-mediated Chinese Cabbage Seed Transformation  

Shin Dong-Il (Department of Biotechnology, Catholic University)
Park Hee-Sung (Department of Biotechnology, Catholic University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 1034-1036 More about this Journal
Abstract
Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.
Keywords
sodium hypochlorite; agroinfiltration; transient expression;
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