• KSBB Journal
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• 제5권3호
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• pp.299-306
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• 1990

대장균과 코리네형 세균간의 shutle vector pECCGI과 pECCD2를 제작하고, plasmid pECCGI과 glycine배지에서 다양한 Corynebacterium glutamicum을 사용하여 전기장 충격법에 의한 형질전환에 있어서 여러 조건을 조사한 결과 세포 현탁액 40ul와 DNA 2ul의 혼합액 사용시 저항 600 obms, 전기장의 세기 12.5kv/cm, DNA양 10ng, 세포수 $4.5$\times$10^8$와 세포회수 시기를 1.0이하의 $A^6^0^0$으로 했을때 $10^6$transformants/ug of DNA의 형질전환 효율을 보였다.

• 한국소음진동공학회논문집
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• 제17권1호
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• pp.17-23
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• 2007

In this paper, the modified direct method employing beam transformation technique is proposed in order to efficiently calculate hydroelastic responses of floating structure. Since the proposed method expresses the displacements of three-dimensional structure with those of transformed beam which leads to small number of equations of motion, the method is numerically efficient compared to the conventional direct method. To verify the efficiency of the proposed method, a 500 m-long floating structure under wave loads is considered in numerical example. Displacements, bending moments, torsion moments and shear forces are calculated and computing time is examined. The results are also compared with those of the conventional direct method.

• 미생물학회지
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• 제36권2호
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• pp.109-111
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• 2000

Lactobacillus casei ssp. casei NCIB 4114 균주로부터 3,5kb의 플라스미드를 분리하여, 이 플라스미드를 함유하는 Escherichia coli-Lactobacillus 셔틀 백터(shuttle vector)들을 만들었다. tu틀 벡터들은 모두 electroporation에 의해 성공적으로 형질전환 되었다. Electroporation의 최적조건은 벡터DNA 1$\mu$g당 $2{\times}10^5$ 형질전환체의 효율이었고, 이들 벡터의 성공적 도입은 이들 벡터의 유산균에서의 음식등급 벡터로의 사용 가능성을 제시하였다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 추계학술대회논문집
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• pp.604-607
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• 2005

The free vibration of solid circular plates has been studied using the differential transformation method(DTM). The effects such as mass at edge and elastic restraints have been considered. In order to avoid the singularity problem at the solid circular center two regularity conditions were applied with respect to the number of circumferential nodal line. The non-dimensional natural frequencies of the general circular plates were obtained for various boundary conditions. The results obtained by this method were compared with previous works. DTM showed fast convergency, accuracy, efficiency and validity in solving vibration problem of general circular plates.

• International Journal of Naval Architecture and Ocean Engineering
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• 제11권1호
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• pp.597-605
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• 2019

This paper proposes a novel method for efficient prediction of joint distributions of heights and periods of nonlinear ocean waves. The proposed novel method utilizes a transformed linear simulation which is based on a Hermite transformation model where the transformation is chosen to be a monotonic cubic polynomial, calibrated such that the first four moments of the transformed model match the moments of the true process. This proposed novel method is utilized to predict the joint distributions of wave heights and periods of a sea state with the surface elevation data measured at the Gulfaks C platform in the North Sea, and the novel method's accuracy and efficiency are favorably validated by using comparisons with the results from an empirical joint distribution model, from a linear simulation model and from a second-order nonlinear simulation model.

• Mycobiology
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• 제49권2호
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• pp.95-104
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• 2021

Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus Aspergillus. The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.

• Journal of Plant Biotechnology
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• 제2권2호
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• pp.89-96
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• 2000

For large DNA fragment transformation in dicots and monocots, BIBAC2 vector system was applied to Arabidopsis thaliana and Oryza sativa L. cv. Jinmi as a model plant, respectively. For Arabidopsis, the Th1 gene in T23L3 BAC clone whose size is about 90 kb was used as the target gene source for transformation. Because T23L3 BAC clone was originally constructed in pBelloBAC11, the target gene was reconstructed into BIBAC2. As the results of reconstruction, 476 colonies were survived in selection medium containing 40 mg/L kanamycin. In colony hybridization analysis, 24 out of 476 colonies exhibited positive signals. In the pulsed-field gel electrophoresis analysis, 11 out of 24 positive clones exhibited the band at the location of 90 kb. In Southern hybridization, positive signal band at the location of 90 kb was observed in all 11 transformants. Using these verified clones, Agrobacterium-mediated transformation was applied to Arabidopsis thaliana th1-201 mutant for genetic complementation test. Twelve thousands T$_1$ seeds were harvested, and antibiotic selection test is being analyzed to verify whether these seeds were transformed. for rice, COR356 that contains 150 kb human genomic DNA in a BIBAC2 vector was used as the target gene. As the results of transformation, 151 out of 210 co-cultivated calli were survived in selection medium containing 5 mg/L hygromycin, and 45 out of 151 survived calli were regenerated into plants. Transformation efficiency was 21.6%. Progeny test using 71 seeds is being analyzed now. These results provide the potential that large DNA fragments can be transferred into both dicots and monocot by Agrobacterium-mediate d transformation system.

• 정보처리학회논문지D
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• 제13D권2호
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• pp.191-198
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• 2006

다양한 환경 속에서 기업이 업무의 효율성과 효과적인 업무처리를 해결하기 위해서 핵심정보시스템으로 워크플로우를 도입하고 있다. 최근에는 워크플로우 확장으로 비즈니스 프로세스 관리(BPM: Business Process Management)가 기업 소프트웨어 시장의 가장 중요한 부분을 차지하고 있다. 비즈니스 프로세스 관리에서 워크플로우는 핵심적인 역할을 수행하고 있지만, 워크플로우 연구의 대부분은 복잡한 업무의 정확한 실행 순서를 찾기 위한 시간적인 제약들에 집중되어 있다. 그런 이유에서 워크플로우 스케줄링의 대부분은 처리해야 할 다양한 자원의 모델링에 집중되었으며, 자원과 연관된 제약조건에서의 워크플로우 스케줄링에 대한 관심은 미흡한 편이다. 본 논문에서는 자원할당 제약조건에 따른 워크플로우 스케줄링에 적합한 CTR(Concurrent Transaction Logic) 기반의 변형 템플릿(Transformation Template)과 논리적 프레임워크를 제안한다. 변형 템플릿은 워크플로우 변형 템플릿(Workflow Transformation Template)과 제약조건 변형 템플릿(Constraint Transformation Template)으로 구성된다. 이 변형 템플릿은 기존의 워크플로우를 자원할당 제약조건 하에서 새로운 워크플로우를 논리적으로 표현하는데 용이하게 사용할 수가 있다.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.211-218
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• 2005

Genetic transformation was carried out by using biolistic gun method. The hypocotyl derived embryogenic calli (explants) of cotton (Gossypium hirsutum L.) cv. Cocker-312 were transformed with a recombinant pGreen II plasmid, in which both, bar (selection marker) and GUS (${\beta}$-glucuronidase) reporter genes were incorporated. Explants were arranged on osmoticum-containing medium (0.5M mannitol) 4 hours prior to and 16 hours after bombardment that was resulted into an increase about >80% for GUS stable expression. 3 days after bombardment, GUS assay was performed, which exhibited, $18.36{\pm}1.00$ calli showed blue spots. The transformed embryogenic calli were cultured on selection medium (@ 6 mg/L basta) for 3 months. The putative transgenic plants were developed via selective somatic embryogenesis (@1.50 mg/L basta); maximum $27.58{\pm}1.25$ somatic embryos were obtained while $17.47{\pm}1.00$ embryos developed into plantlets (@ 0.75mg/L basta). In five independent experiments, up to 7.24% transformation efficiency was recorded. The presence of the transgenes was analyzed by using PCR and southern hybridization analysis. The transgenic plants were developed with in 6-7 months, but mostly transformants were abnormal in morphology.

• International Journal of Aeronautical and Space Sciences
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• 제13권4호
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• pp.458-467
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• 2012

In this study, the linear viscoelastic response of a rectangular laminated plate is investigated. The viscoelastic properties, expressed by two basic spring-dashpot models, that is Kelvin and Maxwell models, is assumed in the range to investigate the influence of viscoelastic coefficients to mechanical behavior. In the present study, viscoelastic responses are performed for two popular equivalent single-layered theories, such as the first-order shear deformation theory (FSDT) and third-order shear deformation theory (TSDT). Compliance and relaxation modulus of time-dependent viscoelastic behavior are approximately determined by Prony series. The constitutive equation for linear viscoelastic material as the Boltzmann superposition integral equation is simplified by the convolution theorem of Laplace transformation to avoid direct time integration as well as to improve both accuracy and computational efficiency. The viscoelastic responses of composite laminates in the real time domain are obtained by applying the inverse Laplace transformation. The numerical results of viscoelastic phenomena such as creep, cyclic creep and recovery creep are presented.