• 한국자원식물학회지
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• 제11권2호
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• pp.188-194
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• 1998

This experiment was conducted to introduce phosphinothricin acetyl -transferase(PAT) gene, resistant to basta and non-selective herbidide, into tobacco(Nicotiana tabacum cv.BY4). For shoot formation,tobacco leaf disks were placed on the MS medium supplemented with 2.0mg/L BA and 0.1mg/L NAA. In this medium condition, tobacco leaf disces were cocultivated with A. tumefaciens MP90 containing NPT IIand PAT resistant to kanamycin and Basta, respectively. Shoots were obtained in the medium containing antibiotics, and those were transferred to rooting medium supplemented with 0.1mg/L NAA and antibiotics. The plants obtaining roots were transplanted into soil. Phenotype of transgenic tobacco plant was mostly as normal plant. However, about 5% was abnormal plant, which did not set seeds. PCR analysis and southern blot were performed to determine transformation. As the results, it was confirmed that PAT gene was stably integrated into tobacco genome.When herbicide, basta, was sprayed to the plants confirmed by PCR, the transgenic plants showed normal growth, whereas normal plants died. Therefore, the result of this experiment show that tobacco transformation for the resistance to basta, non-selective herbicide, was successful because PAT gene was stably integrated into tobacco.

• 한국경제지리학회지
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• 제11권3호
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• pp.389-411
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• 2008

본고는 지역 거버넌스의 변화 측면에서 다임러벤츠의 홈타운 독일의 슈투트가르(Stuttgart)와 피아트의 홈타운 이탈리아 토리노(Torino)의 자동차 클러스터가 경제적 위기에 대응하여 어떻게 재구조화를 추진했는지를 살펴보았다. 이러한 두 지역의 비교를 통해 자동차산업에서 수직 통합에서 수직 분산적 생산방식으로의 변동이 상이한 지역발전경로를 창출하는 미시적 동학을 분석하였다. 이러한 분석에 기반하여 최근의 자동차산업의 생산방식의 변화가 전세계적으로 수렴되는 것이 아니라 각 지역의 유산에 따라 그것은 상이한 경로를 보여주며, 이는 관련된 경제주체 간의 지역 거버넌스와 긴밀히 연계되어 있음을 강조하였다.

• 한국IT서비스학회지
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• 제18권3호
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• pp.75-93
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• 2019

Digital twin technology is one of the key technologies to strengthen the competitiveness of manufacturing industry from the viewpoint of digital transformation in the era of $4^{th}$ industrial revolution. In this research, the important role in using digital twin technology in product design, This paper summarizes and empirically verifies the technical characteristics of digital twins, a key concept in the digital transition of the manufacturing industry. In this study, the technology characteristics of digital twin which is key concept in the digital transformation of manufacturing industry are summarized and empirically validated which factors militate a critical role in the use of digital twin technology in product design which is key area of product development. As a result of analysis, datafication, intellectualization which are characteristics of digital twin technology and task characteristics of product design influence Task Technology Fit (TTF) and Task Technology Fit (TTF) influences Technology (UTAUT) And finally, performance expectancy, effort expectancy, social influence and facilitating conditions affect usage intention.

• 공학교육연구
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• 제25권5호
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• pp.44-54
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• 2022

The purpose of this study is to effectively implement the artificial intelligence education required in the digital transformation era. As we enter the era of the 4th industrial revolution, the demand for a great digital transformation in industry is essential, and the nurturing of manpower is presented as an indispensable relationship in the industrial field based on it. The integration of various new technologies that have emerged from the era of the 4th industrial revolution has the greatest purpose in realizing artificial intelligence technology. As the importance of digital competency in the top curriculum reorganization has been highlighted, artificial intelligence education is necessary even in the curriculum reorganization in 2022, and there is a demand in the educational field that it should be converted into a mandatory education in middle and high schools. Artificial intelligence education according to the demands of the times is to develop an artificial intelligence curriculum in universities by reestablishing systematic artificial intelligence education in universities, setting educational goals, and presenting the goals of artificial intelligence education by major. The main direction of this study is to present the relationship between artificial intelligence and each major in university education, develop a curriculum based on artificial intelligence for each major, and link artificial intelligence software for AI education customized for each major. We would like to present a process that can measure the learning outcomes of AI education.

• Applied Biological Chemistry
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• 제27권
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• pp.56-67
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• 1984

As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.77-85
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• 2004

Development of efficient plant regeneration and genetic transformation protocols (using the Particle Inflow micro-projectile Gun and the shoot-tips as target tissue) of Sorghum bicolor (L.) Moench in terms of expression of the reporter gene, $\beta$-glucuronidase(uidA) is reported here. Two Indian cultivars of sorghum were used in the study, viz. M-35-1 and CSV-15. Plant regeneration was achieved from one-week-old seedling shoot-tip explants via multiple-shoot-clumps and also somatic embryos. The multiple-shoot-clumps were produced on MS medium containing BA (0.5, 1.0 or 2.0 mg/$L^{-1}$), with biweekly subculture. Somatic embryos were directly produced on the enlarged dome shaped expansive structures that developed from shoot-tip explants (without any callus formation) when cultured on MS medium supplemented both with BA (0.5, 1.0 or 2.0 mg/$L^{-1}$) and 2,4-D (0.5 mg/$L^{-1}$). Whereas each multiple-shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing 1.0mg/$L^{-1}$ IBA and successfully transplanted to the glasshouse and grown to maturity with a survival rate of 92%. The plant regeneration efficiency of both the genotypes were similar. After the micro-projectile bombardment, expression of uidA gene was determined by scoring blue transformed cell sectors in the bombarded tissue by an in situ enzyme assay. The optimal conditions comprising a helium pressure of 2200 K Pa, the target distance of 11 cm with helium inlet fully opened and the use of osmoticum have been defined to aid our future strategies of genetic engineering in sorghum with genes for tolerance to biotic and abiotic stresses.

• 한국가금학회지
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• 제34권1호
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• pp.57-76
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• 2007

Marek's disease (MD) is caused by a ubiquitous, lymphotropic alphaherpesvirus, MD virus (MDV). MD has been a major concern in the poultry industry due to the emergence of increasingly virulent strains over the last few decades that were isolated in the face of comprehensive vaccination. MD is characterized by a variety of clinical signs, amongst them neurological symptoms, chronic wasting, and most notably the development of multiple lymphomas that manifest as solid tumors in the viscera and musculature. Much work has been devoted to study MD-induced oncogenesis and genes involved in this process. Among the many genes encoded by MDV, a number have recently been shown to affect the development of tumors in chickens, one protein directly causing transformation of cells (Meq) and another being involved in maintaining transformed cells (vTR). Other MDV gene products modulate and are involved in early lytic in vivo replication, thereby increasing the chance of transformation occurring. In this review, specific genes encoded by MDV that are involved in the initiation and/or maintenance of transformation were briefly summarized, and limits of current vaccination and new control strategies against MD, particularly how modem molecular biological methods may be used to improve strategies to combat the disease in the future, were discussed.

• 한국작물학회지
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• 제54권4호
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• pp.427-432
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• 2009

Seashore Paspalum (Paspalum vaginatum Swartz) is a warm season grass and indigenous to tropical and subtropical regions of coastal areas worldwide. The species is used as feed for cattle and horses and has been very successful for golf courses worldwide. One of the most outstanding characteristics of seashore paspalum is its tolerance to saline soils compared to other warm season turfgrasses. The development of new seashore paspalum cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to product for herbicide resistant seashore paspalum using Arobacterium-mediated transformation and this study is the first report on transformation and herbicideresistant transgenic plants in seashore paspalum. Embryogenic calli were induced from the seeded variety of pseashore paspalum. Embryogenic calli were transformed with Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA3301 with two genes encoding gusA and bar. Transformed calli and plants were selected on medium containing 3 mg/l PPT. PCR detected the presence of the gusA and bar gene, indicating both genes are integrated into the genome of seashore paspalum. A chlorophenol red assay was used to confirm that the bar gene was expressed. By application of herbicide BASTA, the herbicide resistance in the transgenic seashore paspalum plants was confirmed.

• 국제지역연구
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• 제12권3호
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• pp.3-23
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• 2008

본 연구는 28개 체제전환국을 대상으로 정책개혁, 산업구조변화 및 초기조건이 농업생산성에 미치는 효과를 분석하였다. 본 연구의 주요 연구 결과는 다음과 같다. (1) CEE 국가들이 아시아나 CIS의 체제전환국들에 비해 농업부문에서 상대적으로 높은 생산성 향상을 이루었으며, 평균적으로 아시아 및 CEE의 체제전환국이 CIS 국가들 보다 높은 생산성을 보이고 있다. (2) 체제전환국, 특히 CEE 국가들에서 농업부문의 생산성 향상은 주로 기술진보에 의한 것으로 나타났다. (3) 체제전환국에서 정책개혁과 산업구조전환은 농업부문 생산성 향상에 정의 효과를 보였다. (4) 초기조건은 체제전환국 농업부문 생산성 변화에 영향을 미치는 주요 요인의 하나이다.

• 지질학회지
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• 제54권6호
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• pp.657-675
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• 2018

하부 맨틀의 상부에서 관찰되는 섭입된 해양판의 겉보기 두꺼워짐은 과거 연구를 통해 슬랩 좌굴에 의한 것으로 제안되었다. 그러나, 맨틀의 상전이가 슬랩 좌굴에 미치는 영향을 정량적으로 평가하고 이를 규모 법칙으로 검증한 연구는 거의 이루어지지 못하였다. 이 연구에서는 상전이를 고려한 2차원 컴퓨터 섭입 모델링을 수행하여 상전이가 슬랩 좌굴에 미치는 영향에 대해 정량적으로 평가하고 규모 법칙으로 검증하였다. 실험 결과는 410 km 깊이에서 발생하는 감람석-와드슬레이아이트 상전이가 슬랩 좌굴의 발달에 중요한 영향을 미친다는 것을 보였다. 흡열 상전이는 상부 맨틀에서 섭입 슬랩의 침강을 가속시켜 660 km 깊이에 존재하는 불연속면에 빠르게 도달하게 한다. 그러나 660 km 깊이에 존재하는 링우다이트-페로브스카이트+마그네시오우스타이트 상전이는 슬랩 좌굴의 발달에 상대적으로 작은 영향을 미치는데 그 상전이가 섭입 슬랩의 하부 맨틀 침강을 지연시켜 전이대에 섭입한 슬랩을 누적시키기 때문이다. 그럼에도 불구하고 슬랩 좌굴은 규모 법칙을 20% 이내의 오차에서 잘 만족한다. 이처럼 슬랩 좌굴은 맨틀에서 발생하는 보편적인 현상으로써 자바-순다 및 동북 일본 섭입대에서 관찰되는 하부 맨틀의 상부와 전이대에서의 슬랩 좌굴을 잘 설명한다. 또한 백악기 시기 경상 분지가 겪은 주기적인 압축 및 인장이 슬랩 좌굴에 의한 가능성을 암시한다.