• Proceedings of the Microbiological Society of Korea Conference
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• 2000.10a
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• pp.85-91
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• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. It secrets large quantities of proteins or enzymes into the culture medium which makes this organism appealing for the production of heterologous proteins. Recently Electric field-mediated transformation method, electroporation, has been applied to fungal transformation. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, decreasing the culturing time and non-protoplast transformation for the increment of transformation efficiency. Transformants were obtained with electroporation in optimal condition 2,500 voltage, 1,540 ohm and 0.50 capacitance. More than 1,000 transform ants were obtained with 6-10 hrs cultured mycelia without enzyme treatment, called non-protoplast transformation.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.278-285
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• 2008

An efficient transformation method for soybean [Glycine max (L.) Merr.] using meristematic tissues of germinating seeds has been established. The embryonic axes were excised from germinating seeds of Korean soybean cultivar, Iksannamulkong and 0.5-2 cm long segment containing meristematic tissues were prepared by cutting hypocotyl region. The explants were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector with the bar gene as a selectable marker gene and a ${\beta}-glucuronidase$ (GUSINT) reporter gene, and then co-cultured for 7 days on co-cultivation medium (CCM). The meristematic tissues were cultured on shoot induction medium (SIMP6) supplemented with 0.4 mg/l $N_6-benzylaminopurine$ (BAP) and 0.1 mg/l indolebutyric acid (IBA) in the presence of 6 mg/l L-phosphinotricin (PPT) for 2 weeks and the surviving explants were transferred to shoot elongation medium (SEMP6). Transformation was confirmed by Southern blot analysis and the transformation efficiencies ranged from 1.48 to 2.07%. The new modified transformation method was successfully implemented for obtaining several transgenic lines with SMV-CP gene. It is expected that this method could efficiently be used for the transformation of recalcitrant soybean cultivars.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.107-117
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• 2020

Flower industry is now growing due to the development of economy in many countries. Simultaneously, needs from consumers in flower market are varied widely. To satisfy the needs from consumers and deal with a variety of diseases from a lots of pathogens as well as climate change, new elite flower cultivars should be released in flower market. For this purpose, conventional and biotechnological techniques can be employed to make good cultivar. Therefore, this review describes the general overview of flower breeding techniques including cross-hybridization, mutation breeding and genetic transformation systems. Also, breeding systems for ornamentals derived from plant tissue culture such as embryo culture, in vitro fertilization, ovary/ovule culture and haploid production were reviewed. Furthermore, in this study recent development of the generation of new flower cultivars using marker-assisted breeding, plant transformation including particle bombardment and Agrobacterium tumefaciens as well as genome-editing technology were described. This review will be contributed to the development and releasement of new flower cultivars with horticulturally useful traits in the future.

• International Journal of Advanced Culture Technology
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• v.10 no.3
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• pp.377-392
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• 2022

With the development of information and Internet technology, traditional museums have been long followed the trend of integrating innovative technological elements into the changed museums. It is necessary that the museums seize the opportunity of the trend transforming into smart museums, the key is to grasp the characteristics and laws of the Internet era, and use Internet thinking to explore the future development path. However, there are few studies on Internet thinking among the existing results. On the other hand, most of the relevant actual case studies still focus on the micro-level, which has obvious limitations. This paper will start from the current situation and trend, focus on the Palace Museum as a case study object, and discuss the problems and characteristics, so as to put forward the thinking about the development of smart museums in four aspects to explore the optimal path of transformation for smart museums.

• Journal of National Security and Military Science
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• s.4
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• pp.381-418
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• 2006

In present, advanced countries in the world are seen concentring on Military Transformation to create new military capability as there is a shift in Warfare Paradigm. Rapid development of technology enables military system, operational concept and organization innovated. As a result, Revolution in Military Affairs(RMA) that dramatically increased warfighting capability is pursued. Especially, advanced information technology constructs system of systems with abilities of battle visualization, information sharing in battlefield, long-range strike capability that are interoperable and combined. Corresponding to this, engagement methods and organizations are being progressively developed. The purpose of this thesis is overview of newly developing Warfare Paradigm and analysis of tendency of Military Transformation that advanced countries are pursuing preparation to this. Above all, specific aspects and contents of the Warfare Paradigm which emerged by information age were summarized. Subsequently, Military Transformation which provides the basic concepts and principles to the advanced countries in the world for the creation of new military capability was discussed. In conclusion, the tendency of Military Transformation by U.S.A, Japan, China, and Russia surround the Korean peninsular as the major power in military was carefully observed.

• Journal of the Korean Home Economics Association
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• v.44 no.12
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• pp.167-178
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• 2006

The purpose of this study is to analyze the transformed fashion design based on the principles of visual design. Wucius Wong classified the kinds of transformation techniques as internal variation, external variation, extension, superimposition, transfiguration, dislocation, and distortion, which create and modify the shape. And the kinds of shape grammar for transformation are rule deletion, rule addition, and rule transformation, which are applied to interpretate for the change of wearing, the specialization & development of clothes, and the avant-garde styles in subculture groups. The formative meanings of the transformed fashion design are multi-transformation with complexity, formal beauty pretending confusion, and the basis for new trend.

• Journal of the Korean Society for Heat Treatment
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• v.11 no.1
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• pp.35-45
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• 1998

The effects of alloying elements and the conditions of reverse transformation studied treatment on the formation of retained austenite in 0.15C-6%Mn-(Ti, Nb) steels has been studied. The addition of Ti and Nb to 0.15C-6%Mn steel shows no effect on the formation of retained austenite. In case of reverse transformation treatment at various temperatures, the shape of retained austenite was lath type, growing toward the longitudinal and thickness direction with increasing the heat treatment temperatures. The retained austenite formed by the reverse transformation treatment at higher temperature has a lot of stacking faults induced by the internal stress. The retained austenite was stabilized chemically by enrichment of C and Mn in the vicinity of a untransformed austenite and the chemical stability of retained austenite was decreased with increasing the heat treatment temperature and the holding time. It was effective to heat treat at $650^{\circ}C$ in order to obtain over 30vol.% of retained austenite, but more desirable to heat treat at $625^{\circ}C$ for a long time, considering the amount and quality of retained austenite.

• Natural Product Sciences
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• v.14 no.4
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• pp.269-273
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• 2008

Microbial transformation of isoxanthohumol (1), a prenylated flavanone from hops, has resulted in the production of a pair of glucosylated derivatives. The structures of these compounds were elucidated to be (2S)-5-methoxy-8-prenylnaringenin 7-O-${\beta}$-D-glucopyranoside (2) and (2R)-5-methoxy-8-prenylnaringenin 7-O-${\beta}$-Dglucopyranoside (3) based on the spectroscopic analyses.

• International Journal of Advanced Culture Technology
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• v.8 no.1
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• pp.82-88
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• 2020

In the face of recent digital and digital transformation, companies and industries are trying to be agile to adapt and respond to change. Agile paradigm is spreading beyond the boundaries of existing applications such as IT-related projects and software development. In this regard, this study, we analyzed the diffusion of agile paradigm by text mining abstracts of research papers from 2001 to 2019. In addition, we discussed agile transformation in the Fourth Industrial Revolution. Through this study, we confirmed that we are studying agile transformation in various fields such as business environment, corporate organizational culture, manufacturing industry, and supply chain. The results of this study will contribute to understanding the meaning and role of agile as a basic paradigm for digital transformation in the Fourth Industrial Revolution.

• Journal of the Korean Society for Heat Treatment
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• v.12 no.4
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• pp.313-319
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• 1999

The reverse transformation behavior was investigated by DSC analysis in thermomechanically processed Fe-30%Ni-0.35%C alloy. Upon increasing the heating rate from $5^{\circ}C/min$ to $80^{\circ}C/min$, the As point of the ausformed martensite was not changed and the As point of the marformed martensite decreased at reverse transformation. The Af points showed to be constant with increasing the heating rate both in the ausformed martensite and in the marformed martensite. With increasing the deformation degree, the As points of the ausformed martensite and the marformed martensite increased and the Af points appeared to be constant, structures. The enthalpy changes increased with increasing the heating rate, but decreased with increasing the deformation degree in both structures.