• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2000년도 수정란의 위생적 처리·검사 및 특수가축의 수정란이식 기술 심포지움
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• pp.64-75
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• 2000

During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period(1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor(hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.116-116
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• 2002

Further development of reconstructed embryos may be dependent upon the synchronization of donor nucleus and recipient cytoplasm at cell fusion, To control the synchronization of donor and recipient cells, the enucleated MII arrested oocytes are artificially stimulated prior to embryo reconstruction. Destruction of cyclin B results in the exit of cells from M-phase of cell cycle. This study was designed to investigate the effects of single or combined stimulation affected cyclin B1 mRNA and protein levels in mouse oocytes. The oocyte activation was induced by 7% ethanol or 10$\mu\textrm{g}$/$m\ell$ Ca-ionophore without (single) or with (combined) 10$\mu\textrm{g}$/$m\ell$ cycloheximide. Competitive quantitative PCR for cyclin Bl mRNA and western blot analysis for cyclin B1 protein was preformed in mouse oocytes. Cyclin B1 mRNA level was significantly reduced in single (P<0.05) and combined (P<0.05) stimulation groups. However, this level did not change in non-activated group and increased in intact group. Cyclin B1 protein level was also significantly reduced in both single (P<0.05) and combined (P<0.05) stimulation groups. In conclusion, single and combined stimulation induces the degradation of cyclin B1 mRNA and protein after activation in enucleated mouse oocytes.

• 한국통신학회논문지
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• 제27권10C호
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• pp.980-986
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• 2002

하나의 클럭원으로 동기 되는 고속의 데이터 통신 시스템에서, 데이터와 클럭 사이의 다른 라우팅 경로나, 부품들의 다른 전달지연시간 또는 외부 잡음에 의한 데이터나 클럭의 불안정한 위상과 같은 여러 이유들로 인해 데이터를 잃어버릴 수가 있다. 본 논문에서는 이렇게 잃어버린 데이터를 탐지하고 원래의 데이터로 복원하여 보상 출력하는 기능을 갖는 디지털 회로를 제안하고 구현을 기술한다. 특히, 이러한 보상회로는 광 분야등과 같이 고속의 데이터 전송을 위한 통신 시스템에서 강한 안정성을 가지며 BER개선에 상당히 크게 영향을 준다. 이 회로는 Verilog HDL로 구현이 되었으며 통신 및 데이터 전송관련 디지털 ASIC구현에 기본적으로 응용이 가능하다.

• 한국컴퓨터정보학회논문지
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• 제6권4호
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• pp.94-99
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• 2001

패킷 전송망에서의 멀티미디어 데이터 전송은 송수신 단말간에 다양하게 나타날 수 있는 가변적 지연으로 인해 수신지에 도착하는 데이터 패킷간 지연 시간의 차이인 지터와 손실이 발생하므로 수신지 데이터들의 동기화를 이루지 못하게 된다. 특히, 근래의 인터넷과 같은 망의 사용이 급격히 증가함에 따라 나타나는 스파이크(spike)성 지연〔8〕에 대해서는 적절한 동기화 조치가 취해지지 못하고 있다. 본 논문에서는 스파이크성 지연으로 인한 손실을 줄이고 수신지 데이터들의 동기화를 위해 동기 구간을 조정하여 통신망의 급격한 변화에 잘 적응할 수 있도록 하는 적응형 동기화 알고리즘을 제안한다. 또한 임의의 패킷망에서 전형적으로 나타나는 지연 분포 특성을 이용하여 재생 시간을 결정하는 방법을 제안하고, 패킷 손실률과 지연 시간에 대해 성능을 평가한다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.194-199
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• 2007

Effects of recombinant bovine somatotropin (bST) on plasma hormonal concentration, embryo quality, and pregnancy rate were examined during the superovulation and synchronization treatment in donor and recipient cows. Hanwoos (Korean native beef cattle) were treated with controlled internal drug release (CIDR) combined with bST (CIDR+bST) or without bST (CIDR) as donor cows. The embryos recovered from donors were transferred into Holstein recipient heifers treated with bST (CIDR+bST) or without bST (CIDR) for synchronization. The correlation between IGF-I and P4 showed a positive pattern in the CIDR+bST group (r=0.44, p<0.01), but a negative pattern was shown in the CIDR group (r = -0.59, p<0.02) at day 7 of estrous cycles. Although the number of recovered, transferable, and degenerated embryos was not different, quantities of grade 1 (excellent) embryos in CIDR+bST group were significantly higher than those of the CIDR group (p<0.01). The pregnancy rate was higher in the CIDR+bST recipient group compared to CIDR group (p<0.05), when the embryos were recovered from the donors treated with CIDR. However, the pregnancy was maintained highly in both recipient groups, when the embryos were produced by CIDR+bST treated donors. It can be concluded that bST administration combined with CIDR is an effective method for superovulation and synchronization treatment to stabilize plasma hormonal levels, to obtain excellent quality of embryos, and to get higher pregnancy rate.

• 한국수정란이식학회지
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• 제26권2호
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• pp.105-109
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• 2011

Ovulation synchronization (ovsynch) has proved to increase the number of insemination in cattle by overcoming the problems of heat detection. The aim of this study was to do ovsynch in water buffaloes where heat detection is a major reproductive problem and to determine the conception rates after timed artificial insemination (TAI). Twenty cyclic buffaloes at ${\geq}$ 60 days postpartum were selected by examining 24 unobserved estrus buffaloes based on milk progesterone assay (progesterone concentration ${\geq}$ 1.0 ng/ml) from the Mymensingh district of Bangladesh. Ovsynch treatment regimen was started irrespective of the stage of estrous cycle. Gonadorelin (500 ${\mu}g$) was injected intramuscularly at Day 0 followed by Alfaprostol (8 mg) at Day 7. A second injection of Gonadorelin was given at Day 9 and TAI was done with frozen semen from Mediterranean buffalo bulls at 16~20 hours of the second Gonadorelin injection. Milk progesterone ELISA at Day 10~12 post AI confirmed ovulation in 16 out of 20 (80%) buffaloes (progesterone concentration ${\geq}$ 1.0 ng/ml). High progesterone concentration (${\geq}$ 1.0 ng/ml) at Day 10~12 and Day 22~24 of AI showed pregnancy in six out of 20 (30%) buffaloes. Pregnancy was further confirmed by ultrasonography at Day 40 in these six buffaloes. In conclusion, ovsynch followed by TAI could be applied in cyclic buffaloes for overcoming the estrus detection problems; however, more studies are needed to increase the conception rate.

• 한국정보전자통신기술학회논문지
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• 제8권6호
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• pp.470-477
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• 2015

본 논문은 통신 프로토콜의 적합성 시험시 하위 시험기와 상위 시험기간의 직접적인 상호 작용이 불가능한 분산 시험 환경에서 외부 동기화와 입출력 운용비용을 고려해야 할 경우 적용될 수 있는 최적화된 동기적 시험 항목 생성을 위한 새로운 방법을 제시한다. 이 방법은 세 가지 단계의 절차로 구성되어 있는데, 첫 번째 단계는 시험 구현물의 각 상태로부터 나오는 모든 입출력 짝들의 유일성을 기술하는 상태인식 표를 생성한다. 둘 째 단계에서는 이 상태인식 표로부터 각 상태의 동기화된 상태인식 열을 구성한다. 마지막 단계에서는 시험 구현물의 시험에지들과 각 상태의 동기화된 상태인식 열들을 이용하여 최적화된 동기적 시험열들을 생성한다. 본 논문의 방법은 원거리 시험기들을 사용하는 프로토콜 시험 구조에서 기 결정된 시험 항목을 적용할 때 시험 대상과 이들 시험기들간에 발생할 수 있는 동기화의 유용한 해결 기법으로 사용될 수 있다.

• 한국수정란이식학회지
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• 제18권3호
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• pp.187-193
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• 2003

본 연구는 한우에 GnRH ＋ PGF$_2$$\alpha$＋GnRH (Ov-synch)를 처리하여 배란동기화를 시켰으며, 2차 GnRH 투여후 배란시간, 수태율, 계절별 수태율, 산차별 수태율을 조사하였으며, 시험축은 2산 이상의 개체를 무작위로 선발하여 실험에 공시하였으며, 배란동기화 처리후 1회 인공수정을 실시하고 수태율을 환산하였다. 호르몬 처리방법으로는 GnRH ＋ PGF$_2$$\alpha$ ＋ Gn-RH(Ov-synch)법을 이용하였으며, 배란시간의 조사는 초음파를 이용하여 2차 GnRH 투여 후 24시간부터 31시간까지 2시간 간격으로 난소를 촬영하여 배란 여부를 조사하였다. 1. 배란동기화 처리후 24시간부터 31시간까지 2시간 간격으로 난소의 상태를 확인한 결과 28∼30시간 사이에 80%(20/25)로 가장 많이 배란된 것으로 나타났다. 2. 수태율에 있어서는 계통별 1회 수정 수태율은 고급육 계통이 48.1%(38/79)로 다소 높게 나타났다. 3. 산차에 따른 수태율은 1∼2, 3∼4산차에서 각각 44.3, 55%로 나타났다. 4. 계절별로는 여름보다는 봄과 가을에서 47.3%로 다소 높은 경향이였다.

• 한국수정란이식학회지
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• 제29권4호
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• pp.321-325
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• 2014

The present study was performed on farm animals to test the effectiveness of progesterone-releasing intravaginal device (Cue-Mate$^{(R)}$ 1.56 g) and injection of prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) for synchronization estrus in Hanwoo cattle. The cattle were at random stage of the estrus cycle. The cows were artificially inseminated at day 7 after Cue-Mate withdrawal, using commercial semen from Korean native bulls. There was a season effect on the estrus synchronization rate. It was higher in spring (94.3%) followed by winter (93.3%), autumn (90.4%) and summer (67.2%). In summary, The results of this study revealed that season has influences on estrus behavior of cattle with no significant effect on pregnancy rate. In summary, we suggest summer reproductive management to alleviate the effects of heat stress. It should be based on intensive cooling combined with hormonal treatment. Given that different subgroups of cows benefit differently from the treatments, selective hormonal administration should be considered.

• 한국수정란이식학회지
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• 제2권1호
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• pp.33-35
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• 1987

This study was carried out to investigate the seasonal effect on the recovery rate of embryos in donors and on the conception rate in recipients following embryo transfer. The results obtained from this experiment were summarized as follows: 1. The ovulation point in winter and summer was 28.6 and 28.6, respectively. There was no difference in ovulation point between two seasons. More embryos recovered in the winter (27,0) than the summer (20.9). 2. The number of CL, unruptured follicle, hemorrhagic follicle, young born and pregnancy rate in the winter were 6.0,4.8,1.5,1.8 and 75%, and those in the summer were 2.9,5.7, 3.8, 2.2 and 46.7%, respectively. The rate of synchronization of recipients in the winter showed better results than that in the summer.