Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Synchronization Rate, Size of the Ovulatory Follicle, and Pregnancy Rate after Synchronization of Ovulation Method in Hanwoo

한우에 Ov-synch 처리시 배란시기와 수태율 구명에 관한 연구

  • Published : 2003.12.01
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Abstract

Recently a protocol was developed that precisely synchronizes the time of ovulation in Hanwoo. Cows were treated with GnRH on Day 0, PGF$_2$$\alpha$ 7 d later, GnRH 2 d later, and then time-inseminated approximately 24 h after this second treatment with GnRH. Ovarian morphology was monitored cows by trans-rectal ultrasonography 6.5MHz linear transrectal probe(Sonovet - 600., Medison co. Korea) from 24 hr to 31 hrs after second GnRH injection. The result obtained summarized as follows - 1. Induced ovulation were 24 to 31hr after the second GnRH injection, but high induced ovulation was 28hr. 2. Conception rate with HML(High meat lin) and HIL(High milk lin) treatment were 48.1％(38/79) and 43.9％(40/91), respectively. 3. Conception rate of 1∼2 parity and 3∼4 parity was 44.3％ and 55％, respectively. 4. Conception rate of spring, autumn was more increased, 47.3％ than summer.

본 연구는 한우에 GnRH ＋ PGF$_2$$\alpha$＋GnRH (Ov-synch)를 처리하여 배란동기화를 시켰으며, 2차 GnRH 투여후 배란시간, 수태율, 계절별 수태율, 산차별 수태율을 조사하였으며, 시험축은 2산 이상의 개체를 무작위로 선발하여 실험에 공시하였으며, 배란동기화 처리후 1회 인공수정을 실시하고 수태율을 환산하였다. 호르몬 처리방법으로는 GnRH ＋ PGF$_2$$\alpha$ ＋ Gn-RH(Ov-synch)법을 이용하였으며, 배란시간의 조사는 초음파를 이용하여 2차 GnRH 투여 후 24시간부터 31시간까지 2시간 간격으로 난소를 촬영하여 배란 여부를 조사하였다. 1. 배란동기화 처리후 24시간부터 31시간까지 2시간 간격으로 난소의 상태를 확인한 결과 28∼30시간 사이에 80%(20/25)로 가장 많이 배란된 것으로 나타났다. 2. 수태율에 있어서는 계통별 1회 수정 수태율은 고급육 계통이 48.1%(38/79)로 다소 높게 나타났다. 3. 산차에 따른 수태율은 1∼2, 3∼4산차에서 각각 44.3, 55%로 나타났다. 4. 계절별로는 여름보다는 봄과 가을에서 47.3%로 다소 높은 경향이였다.

Keywords

References

  1. Bartolome JA, Archbald LF, Morresey P, Hernandez J, Tran T, Kelbert D, Long K, Risco CA and Thatcher WW. 2000. Comparison of synchronization of ovulation and induction of estrus as therapeutic strategies for bovine ovarian cysts in the dairy cow. Theriogenology, 53: 815-825 https://doi.org/10.1016/S0093-691X(99)00276-9
  2. Bartolome J, Hernandez J, Sheerin P, Luznar S, Kelbert D, Thatcher WW and Archbald LF. 2003. Effect of pretreatment with bovine somatotropin (bST) and/or gonadotropin-releasing hormone (GnRH) on conception rate of dairy cows with ovarian cysts subjected to synchronization of ovulation and timed insemination. Theriogenology, 59:1991-1997 https://doi.org/10.1016/S0093-691X(02)01286-4
  3. Bastidas P and Randel RD. 1987. Seasonal effects on embryo transfer results in Brahman cows. Theriogenology, 28(4):531-540 https://doi.org/10.1016/0093-691X(87)90258-5
  4. Frick PM, Guenther JM and Wiltbank MC. 1998. Efficacy of decreasing the dose of GnRH used in a protocol for synchronization of ovulation and timed ai in lactating dairy cows. Theriogenology, 50:1275-1284 https://doi.org/10.1016/S0093-691X(98)00226-X
  5. Geary TW, Whittier JC, Hallford DM and MacNeil MD. 2001. Calf removal Improves conception rates to the Ovsynch and CO-Synch protocols. J. Anim. Sci., 79(1):1-4 https://doi.org/10.2527/2001.7911
  6. Lemastea JW, Yelich JV, Kempfer JR, Fullenwider JK, Barnett CL, Fanning MD and Selph JF. 2000. Effectiveness of GnRH plus prostaglandin $PGF_2a$ for estrus synchronization in cattle of Bos indicus breeding. J. Anim. Sci., 79(2): 309-16
  7. Murugavel K, Yaniz JL, Santolaria P, Lopez-Bojar M and Lopez-Gatius F. 2003. Luteal activity at the onset of a timed insemination protocol affects reproductive outcome in early postpartum dairy cows. Theriogenology, 60: 583-593 https://doi.org/10.1016/S0093-691X(03)00047-5
  8. Momcilovic D, Archbald LF, Walters A, Tran T, Kelbet D, Risco C and Thatcher WW. 1998. Reproductive performance of lactating dairy cows treated with gonadotrophin-releasing hormone (GnRH) and/or prostaglandin $F_2a(PGF_2a)$ for synchronization of estrus and ovulation. Theriogenology, 50:1131-1139 https://doi.org/10.1016/S0093-691X(98)00214-3
  9. Neglia G, Gasparrini B, Palo RD, Rosa CD, Zicarelli L and Campanile G. 2003. Comparison of preganancy rates with two estrus synchronization protocols in Italian Mediterranean Buffalo cows. Theriogenology, 60:125-133 https://doi.org/10.1016/S0093-691X(02)01328-6
  10. Pursley JR, Mee MO and Wilbank MC. 1995. Synchronization of ovulation in dairy cows using $PGF_2a$ and GnRH. Theriogenology, 44:915-923 https://doi.org/10.1016/0093-691X(95)00279-H
  11. Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA and Anderson LL. 1997. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J. Dairy. Sci., 80:295-300 https://doi.org/10.3168/jds.S0022-0302(97)75937-X
  12. Pursley JR, Silcox RW and Wiltbank MC. 1998. Effect of time of artificial insemination on pregnancy rates, calving rates, pregnancy loss and gender ratio after synchronization of ovulation in lactating dairy cows. J. Dairy. Sci., 81:2139-2144 https://doi.org/10.3168/jds.S0022-0302(98)75790-X
  13. Shea BF, Janzen RE and McDennand DP. 1984. Seasonal variation in response to stimulation and related embryo transfer procedures in alberta over a nine year period. Theriogenology, 21(1):186-195 https://doi.org/10.1016/0093-691X(84)90316-9
  14. Stevenson JS, Kobayashi Y and Thompson KE. 1999. Reproducive performance of dairy cows in various programmed breeding systems including ovsynch and combinations of gonagonadotropin- releasing hormone and prostaglandin F2a. J. Dairy. Sci., 82:506-515 https://doi.org/10.3168/jds.S0022-0302(99)75261-6
  15. Yavas Y, Johnson WH and Walton JS. 1999. Modification of follicular dynamics by exogenous FSH and progesterone, and the induction of evaluation using hCG in postpartum beef cows. Theriogenology, 52:949-963 https://doi.org/10.1016/S0093-691X(99)00185-5