• Proceedings of the KSME Conference
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• 2000.11b
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• pp.272-277
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• 2000

The heat transfer characteristics of confined turbulent slot jet impingement on the flat plate with square rods(turbulence promoter) have been experimentally investigated at different nozzle configuration. The effects of jet Reynolds number (Re=3900, 5800, 7800, 9700), dimensionless slot-to-plate distance(H/B=4, 6, 8) and clearance(c) between square rods and the plate were examined. Measurement of heat transfer rate were conducted using naphthalene sublimation technique. When square rods were inserted over the heat transfer surface, heat transfer rate was slightly increased in the wall jet region and the sharp-edged orifice nozzle was heigher than squared orifice nozzle.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.119-124
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• 2000

The heat transfer characteristics of confined turbulent slot jet impingement on the flat plate with square rods(turbulence promoter) have been experimentally investigated. The effects of jet Reynolds number (Re=3900, 5800, 7800, 9700), dimensionless slot-to-plate distance(H/B=4, 6, 8) clearance(c) between square rods and the plate, and the length(d) of a side of the square rod were examined. Measurement or heat transfer rate were conducted using naphthalene sublimation technique. When square rods were inserted over the heat transfer surface, heat transfer rate was slightly increased in the wall jet region.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.541-547
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• 1999

Constructions of a transfer vector and a recombinant baculovirus using the thymidine kinase gene of the Herpes simplex virus type 1 strain F (HSV -1) were carried out. Newly cloned transfer vector, pHcgXIIIB, was constructed by insertion of the glycoprotein gX gene signal peptide sequence of Pseudorabies virus into the baculovirus vector pHcEV-IV. The gX sequence was inserted just downstream from the promoter for the polyhedrin gene of the Hyphantria cunea nuclear polyhedrosis virus (HcNPV). HSV-1 thymidine kinase(tk) gene (1.131 kb) was used as a candidate gene for transferring into the baculovirus expression system. The tk gene was inserted into a BamHI site downstream from the gX sequence-promoter for the polyhedrin gene in the pHcgXIIIB transfer vector and was transferred into the infectious lacZ-HcNPV expression vector. Recombinant virus was isolated and was named gX-TK-HcNPV. The recombinant virus produced a 45 kDa gX-TK fusion protein in Spodoptera frugiperda cells, which was confirmed by Western blot analysis. Microscopic examination of gX-TK-HcNPV-infected cells revealed normal multiplication. Fluorescent antibody staining indicated that the gX-TK fusion protein was present in the cytoplasm. These results indicated that the transfer vector successfully transferred the gX-tk gene into the baculovirus expression system.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.4
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• pp.1481-1490
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• 1996

This study is intend to improve flow pattern within evaporator, which is low quality and low mass flux, by installing narrow horizontal annular crevice so that enhance heat transfer coefficient. The motive, which made to study heat transfer enhancement by using narrow annular crevice, came from capillary phenomena and pumping force of generating vapor on refrigerant boiling. Tests were run about 5 models of turbulence promoter with CFC-12, in the range of evaporating temperature (15.deg. C), mass flux (50 to 100 kg/m$\^$2/s), heat flux (3.4 to 6.7 kW/m$\^$2/), quality (0.1 to O.5). It is observed that flow pattern within evaporator is changed closely to semi-annular flow or annular flow, of which refrigerant liquid is reached to the upper side of tube by using narrow annular crevice. When the narrow annular crevice is installed in the evaporator tube, local heat transfer coefficient is generally more improved than that of smooth tube. That fact is according to observed result of flow pattern. It is learned that narrow annular crevice has more efficiency at a low mass flux. At the TP-5, enhancement of heat transfer rate is about 170% compare to that of smooth tube on a low mass flux (50 kg/m$\^$2/s), and it is about 134% on a high mass flux (100 kg/M$\^$2/S), so that we know that it is on a very high condition.

• Journal of Energy Engineering
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• v.21 no.2
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• pp.187-193
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• 2012

In this study, it was experimentally investigated the effect of the clearances distance between the rod and the impinging plate on characteristics of the thermal flow. For the heat transfer enhancement of wall jet region, the right triangle and the square rods were arranged in front of the impinging plate with various clearance distances. As results, the heat transfer enhancement rate of potential core region at H/B=2 was higher than that of transition region at H/B=10. In this experiment range, the maximum heat transfer enhancement rate was about 46 % higher at the square rod with H/B=2 and C=1mm compared with the flat plate. The heat transfer enhancement rate of the right triangle rod was on average about 3 to 8 % higher than that of the square rod, regardless of the clearance.

• Journal of Ceramic Processing Research
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• v.19 no.5
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• pp.372-377
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• 2018

MgO or gadolinium-doped ceria (GDC, $Ce_{0.9}Gd_{0.1}O_{2-{\delta}}$) was added as a promoter to improve the oxygen transfer kinetics of $MgMnO_3$ oxygen carrier material for chemical looping combustion. Neither MgO nor GDC reacted with $MgMnO_3$, even at the high temperature of $1100^{\circ}C$. The average oxygen transfer capacities of $MgMnO_3$, 5 wt% $MgO-MgMnO_3$, and 5 wt% $GDC-MgMnO_3$ were 8.74, 8.35, and 8.13 wt%, respectively. Although the addition of MgO or GDC decreased the oxygen transfer capacity, no further degradation was observed during their use in 5 redox cycles. The addition of GDC significantly improved the conversion rate for the reduction reaction of $MgMnO_3$ compared to the use of MgO due to an increase in the surface adsorption process of $CH_4$ via oxygen vacancies formed on the surface of GDC. On the other hand, the conversion rates for the oxidation reaction followed the order 5 wt% $GDC-MgMnO_3$ > 5 wt% $MgO-MgMnO_3$ >> $MgMnO_3$ due to morphological change. MgO or GDC particles suppressed the grain growth of the reduced $MgMnO_3$ (i.e., (Mg,Mn)O) and increased the specific surface area, thereby increasing the number of active reaction sites.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.1366-1371
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• 2008

Four simulation models of plastic plate heat exchangers are designed and simulated. The flat plate type heat exchanger is designed as the reference model in order to evaluate how much thermal performance increases. The turbulence promoter type heat exchanger is fabricated with cylindrical-type vortex generators and rib-type turbulence promoters. The corrugate type is obtained from the conventional stainless steel compact heat exchangers, which are called the herringbone-type compact heat exchangers. The dimple type heat exchanger has a number of dimples on its surface. In this study, the flow and heat transfer characteristics of the plastic plate heat exchanger are investigated using numerical simulation and compared with experimental results. The flows are assumed as a three-dimensional, incompressible and turbulent model. The standard k-$\varepsilon$ model is used as the turbulent flow modeling, the SIMPLE algorithm is used to treat the coupling between pressure and velocity, and first order upwind scheme is used for discretization of momentum, turbulent and energy. The computational analysis and experimental results both show that the friction coefficient and Nu number is highest in the corrugate type.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.1
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• pp.152-161
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• 2004

The thermal load is a very important problem to be solved in many industrial systems including the electronic equipment. Impinging Jets have been known to provide a large heat transfer rates on surface for many years. The turbulence enhancement of fluid flow is requested for the efficiency elevation of heat transfer. A study on flow fields by rods attached to the wall surface as a promoter of turbulence enhancement has been carried out. The exact analysis on chracteristics of impinging jet field is requested to obtain the optimum design of the impinging jet system. By visualizing the flow field and processing the high digital image by computer PIV can afford exact data on the velocity vector kinetic energy and turbulence intensity in the complex turbulence field. In this study. three kinds of rod shape such as square. triangle. and semicircle are selected as the turbulence promoter. Nozzle diameters are 10mm. 17mm. and 23mm. And the analysis of the flow characteristics due to the above rods is carried out at Re No. 2.000, 3.000. and 4,000 by PIV measurement. It is clarified that the rod setup is very useful to obtain the turbulence enhancement and the turbulence intensity according to the shapes of rod appears large in order of the shapes of rod such as square 〉 triangle 〉semicircle.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.37-43
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• 1998

The highest transformation frequency was observed when cotyledonary and hypocotyl explants of flowering cabbage (Brassica oleracea var. acephala DC) 'Eunbae' were cultured on shoot induction medium without kanamycin for 1 day, then cocultured with Agrobacterium tumefaciens LBA4404;;pGA1036 harboring tomato inhibitor II promoter and $\beta$-glucuronidae (GUS) fusion gene for 3 days. These explants were transferred to MS medium containing 20 mg/L kanamycin, 500 mg/L carbenicillin, and 1 mg/L BA. The explants were subsequently subcultured every 2 weeks. Incorporation of the GUS gene into flowering cabbage was confirmed by PCR analysis of DNA. Southern blot analysis showed that ECL-labeled GUS gene was hybridized to the expected amplified genomic DNA fragment of about 366 bp from transgenic flowering cabbage. Histochemical analysis based on the enzymatic activity of the GUS protein indicated that PI-II promoter activity was sysmatically associated with vascular tissue in wonded as well as in non-wounded leaves, petioles and stems, but not in roots. Partial wounding with razor blade showed not systemic induction but partial induction.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1719-1727
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• 2014

In the present study, whispovirus immediate early 1 promoter (ie-1) was used to initiate surface expression of the hemagglutinin (HA) protein of Egyptian H5N1 avian influenza virus (AIV) by using the baculovirus expression vector system. The HA gene and whispovirus ie-1 promoter sequence were synthesized as a fused expression cassette (ie1-HA) and successfully cloned into the pFastBac-1 transfer vector. The recombinant vector was transformed into DH10Bac competent cells, and the recombinant bacmid was generated via site-specific transposition. The recombinant bacmid was used for transfection of Spodoptera frugiperda (Sf-9) insect cells to construct the recombinant baculovirus and to induce expression of the HA protein of H5N1 AIV. The recombinant glycoprotein expressed in Sf-9 cells showed hemadsorption activity. Hemagglutination activity was also detected in both extra- and intracellular recombinant HAs. Both the HA and hemadsorption activities were inhibited by reference polyclonal anti-H5 sera. Significant expression of the recombinant protein was observed on the surface of infected insect cells by using immunofluorescence. SDS-PAGE analysis of the expressed protein revealed the presence of a visually distinguishable band of ~63 kDa in size, which was absent in the non-infected cell control. Western blot analysis confirmed that the distinct 63 kDa band corresponded to the recombinant HA glycoprotein of H5N1 AIV. This study reports the successful expression of the HA protein of H5N1 AIV. The expressed protein was displayed on the plasma membrane of infected insect cells under the control of whispovirus ie-1 promoter by using the baculovirus expression vector system.