• Analytical Science and Technology
• /
• v.28 no.6
• /
• pp.460-466
• /
• 2015

An isotope dilution liquid chromatography tandem mass spectrometry was developed as a primary method for the quantitative analysis of cholesterol in infant formula. Cholesterol-d₄ was used as an internal standard and spiked into the infant formula sample. In order to release cholesterol out of cholesteryl ester, which is cholesterol bound to fatty acids in infant formula, saponification was carried out. Saponification conditions were optimized with heating temperature, reaction time and the concentration of KOH. The optimum conditions were as follows; heating temperature was 70 ℃, reaction time was 180 min and the concentration of KOH was 0.8 mL of 8 M KOH for about 0.1 g infant formula sample. Extraction of cholesterol out of sample solution was carried out with hexane uisng liquid-liquid extraction. Chromatographic analysis was carried out using Phenomenex Kinetex C₁₈ column. Mobile phase was 0.1% acetic acid in methanol/water (v/v, 99/1) and flow rate was 0.3 mL/min. Cholesterol and cholesterol-d₄ were monitored at mass transfer m/z 369/259 and 373/263 respectively. Reproducibility of the method was evaluated to be 0.23% of the measurement result. The expanded uncertainty of the measurement result of cholesterol in infant formula was approximately 1.9% at a 95% confidence level. NIST standard reference material having certified values of cholesterol in infant formula, was analyzed in order to verify this method. The ID-LC/MS/MS results were well agreed with the certified values of NIST SRM within the uncertainty.

• KSBB Journal
• /
• v.11 no.1
• /
• pp.22-29
• /
• 1996

Immobilized bioprocess was carried out for continuous production of cyclosporin A (CyA) produced intracellularly as a secondary metabolite by a filamentous fungus, Tolypocladium inflatum. Immobilization procedure for entrapping conidiospores of the producer was significantly simplified by use of a modified immobilization technique. A newly-designed immobilized perfusion reactor system (IPRS) showed good process benefits as demonstrated by the role of the high density immobilized cells as an efficient biomass generator, continuously supplying highly active CyA-producing free cells (1.0g/$\ell$/hr) even at very high dilution rate ($0.1hr^{-1}$). IPRS bioprocess was possible since efficient decantor system developed in our laboratory separated the sloughed-off free cells from the immobilized biomass effectively, thus overcoming wash-out phenomenon frequently encountered in continuous free cell cultures. Furthermore the released-free cells remaining in the bulk solution did not appear to cause substrate mass transfer limitation which was often experienced in suspended mycelial fungal cell fermentations. The primary reason for this was that the suspension broth of the IPRS mainly consisted of roundshaped short mycelial fragments and conidiospores, still remaining Newtonian even at high cell density. In parallel with IPRS bioprocess development, other key factors to be considered necessarily for significant increase in CyA productivity would be strain improvement and medium optimization for the immobilized cells.

• Journal of Korean Society of Steel Construction
• /
• v.14 no.4
• /
• pp.557-566
• /
• 2002

This paper summarized the results of a full-scale cyclic seismic testing on four reduced beam section (RBS) steel moment connections. Specifically, these tests addressed a bolted web versus a welded web connection and strong versus medium panel zone (PZ) strength as key test variables. Specimens with medium PZ strength were designed to promote balanced energy dissipation from both PZ and RBS regions, in order to reduce the requirement for expensive doubler plates. Both strong and medium PZ specimens with welded web connection were able to provide sufficient connection rotation capacity required of special moment-resisting frames. On the other hand, specimens with bolted web connection performed poorly due to premature brittle fracture of the beam flange at the weld access hole. Unlike the case of web-welded specimens, specimens with cheaper bolted web connection could not transfer the actual plastic moment of the original (or unreduced) beam section to the column. No fracture occurred within the beam groove welds of any connection in this testing program. If fracture within the beam flange groove weld is avoided by using quality welding procedure as in this study, the fracture issue tends to move into the beam flange base metal at the weld access hole. Supporting analytical study was also conducted in order to understand the observed base metal fracture from the engineering mechanics perspective.

• Analytical Science and Technology
• /
• v.6 no.1
• /
• pp.1-8
• /
• 1993

The purpose of this study is to investigate the elution behavior of monosubstituted phenols and benzenes in micellar liquid chromatographic system, $C_{18}$ column-anionic surfactant, sodium dodecyl sulfate(SDS). The partition coefficients between the micellar pseudophase-water and modified stationary phase-water are calculated by the relationship between solute retention and micellar mobile phase(SDS) composition. The free energy of transfer of solute from water to micelle is also calculated from these values. There is a direct correlation between the hydrophobicity parameters in MLC and corresponding partition data for 1-octanol-water, which indicates that the hydrophobicity of molecules plays an important role in the partition for both systems and that quantitative structure activity relationships(QSAR) are available from studies on micellar partition. The other purpose of this study is to investigate methylene selectivity of alkyl homologous series through correlation between retention and the number of carbons. The correlation between hydrophobicity parameters in MLC and 1-octanol-water partition data was also observed when n-propanol was as a modifier in the mobile phase.

• The Journal of Engineering Geology
• /
• v.17 no.3
• /
• pp.419-424
• /
• 2007

The contents were investigated by the monitoring survey from the soils and tailings caused by numerous abandoned mines in Korea. Cause heavy metals due to abandoned metal mines are raising significant environmental problems. But it is an important key such as a leaching and a transfer mechanism to evaluate contamination levels caused by abandoned mines. In this study the column test was carried in order to calculate a leaching level from soils and tailings. It was demonstrated that the leaching of Pb, Cd and Mn was expressed with similar behaviors and that of As and Cu was expressed with similar behaviors. For Zn, the leaching behavior was shown a serious leaching level with 40 mg/kg during the 45days. This was explained by Zn high contents of soils Zn in a natural world and ORP conditions where the leaching of Zn was occurred easily. Hence it was necessary that the survey of ORP was a key as well as total contents for the management of abandoned metal mines. We could estimate the chemical forms of heavy metals using the physical index such as ORP and pH and reduce the risk from heavy metals caused by abandoned metal mines.

• Clean Technology
• /
• v.24 no.1
• /
• pp.50-54
• /
• 2018

In this work, N-methyl-2-pyrrolidone (NMP) was added into diethylenetriamine (DETA) aqueous solution for high $CO_2$ loading via phase splitting of absorbents during $CO_2$ absorption. Immiscible two phases were formed in the range of more than 30 wt% of NMP in 2 M DETA + NMP + water absorbents because of low solubility of DETA-carbamate in NMP solution. As the composition of NMP in the absorbents increased, the difference of $CO_2$ loading between each phase increased and the volume of bottom phase decreased. In $CO_2$ absorption in packed column by 2 M DETA + NMP + water absorbents, the absorption rate decreased in the range of more than 40 wt% of NMP. It is due to the increasing of mass transfer resistance in liquid film of absorbents at the high concentration of NMP. DETA + NMP + water absorbent is expected as the promising one for reducing the regeneration energy of absorbents according to volume reduction of $CO_2-rich$ phase.

• Journal of Korean Society of Steel Construction
• /
• v.15 no.6 s.67
• /
• pp.683-691
• /
• 2003

Recently, the steel has been increaseingly used as an integrated part of high-rise buildings, which often composed of steel structures, steel reinforced concrete structures and composite structures. The steel base is designed to transfer the stresses induced from steel column to the reinforced concrete footing through the base plate. However, in the design of steel structures and steel reinforced concrete structure, it is generally difficult to evaluate the bearing strength of the steel base subjected to large axial force. Furthermore, the material used in steel base is quite different from those used in other connections and a load transferring mechanism of steel base is very complicated in nature. Therefore, a special attention must be placed in design and construction of steel base. In generally, the bearing strength test and research of the steel base subjected to concentrated load are carried out. But, in the design of the structures, uniaxial eccentric load is loaded to the steel base of the steel structures. In this research, the bearing strength and the me of failure considering eccentric loads and eccentric length, were experimented when eccentric load is loaded to the steel base of steel structures. Based on the test results, a basic design reference is suggested for a reasonable design of steel structures, steel reinforced concrete structures and composite structures.

• Korean Journal of Remote Sensing
• /
• v.24 no.6
• /
• pp.535-549
• /
• 2008

Vicarious calibration for the satellite sensor relies on simulated TOA (Top-of-Atmosphere) radiances over various targets. In this study, TOA visible radiance was calculated over ocean targets which are located in five different regions over the Indian and Pacific ocean, and its possible use for the satellite sensor calibration was examined. TOA radiances are simulated with the 6S radiative transfer model for the comparison with MODIS/Terra and SeaWiFS measurements. Geometric angles and sensor characteristics of the reference satellites were taken into account for the simulation. AOT (Aerosol Optical Thickness) from MODIS/Terra, pigment concentrations from Sea WiFS, and ozone amount from OMI measurements were used as inputs to the model. Other atmospheric input parameters such as surface wind and total column water vapor were taken from NCEP/NCAR reanalysis data. The 5-day averaged radiances over all targets show that the percent differences between simulated and observed radiances are within about ${\pm}5%$ in year 2005, indicating that the calculated radiances are in good agreement with satellite measurements. It has also been shown that the algorithm can produce the SeaWiFS radiances within about ${\pm}5%$ uncertainty range. It has been suggested that the algorithm can be used as a tool for calibrating the VIS bands within about 5% uncertainty range.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.2
• /
• pp.67-75
• /
• 2019

The diaphragm used for CFT columns has a small amount of steel to be used, but has a disadvantage that welding is difficult and openings are required because the steel tube and four sides must be welded. The improved diaphragm to be examined in this study was cut into four corners by cutting the center hole for concrete filling. In the improved diaphragm, the width of the center hole is the same as that of the previous diaphragm, but the width of the diaphragm contacting the steel tube is reduced, thereby reducing the welding length by about 70% compared to the previous diaphragm. The in-plane strain of each specimen was analyzed when the same load was applied to the interior diaphragm through a simple tensile test. Using the general FEM program(ANSYS 19.2), the analysis was performed under the same conditions as the actual simple tensile test, and the load transfer between the improved diaphragm and the previous diaphragm was compared. When the width of the diaphragm is equal to or smaller than the flange width, stress is concentrated from the end of the diaphragm, and when the flange width is larger, stress is concentrated at the center.

• Economic and Environmental Geology
• /
• v.54 no.4
• /
• pp.483-494
• /
• 2021

This study was carried out to investigate the feasibility of reducing clean cover soil using a flooded column test in arsenic-contaminated farmland reclamation of abandoned coal mine area that shows generally low or about worrisome level (25 mg/kg) of Korea soil environment conservation act unlike abandoned metal mine. During the monitoring period of soil solution for 4 months, chemical properties (pH, EC, ORP, Fe, Mn, Ca, and As) in each layer (clean soil cover and contaminated/stabilized soil) showed different variation. This result revealed that soil solution in stabilized or contaminated soil rarely affected that in cover soil. Whether stabilized or not, arsenic concentrations in the rice roots grown in the soil covers with the thickness of 40 cm decreased by 98% in compared with the that grown in the control soil. In case of the soil covers with 20 cm thickness on stabilized soil, it decreased by 80% and this was 22 percentage point higher than when the soil of lower layer was not stabilized. Thus, reducing clean cover soil could be possible in contaminated farmland soil reclamation if appropriate stabilization of contaminated soil is carried.