• 한국식품영양과학회지
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• 제32권3호
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• pp.325-337
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• 2003

한국인의 상용 가공식품 중 tram 지방산 함량이 많고 섭취빈도가 상대적으로 높은 41종을 선정하여, trans 지방산 이성체의 종류 및 함량을 GC/MS를 사용하여 분석하였다. HP-23 cis/trans FAME capillary column을 사용하였으며, 검출된 peak의 동정은 GC/MS spectrum을 검색하여 동일분자량 여부로 확인하였다. 마가린류는 trans 지방산 함량이 지방 100g당 4.75~25.21g으로 다양한 수준을 보였다. 마가린의 주요 trans 지방산 이성 체는 C18:1 $\Delta$9t로 모든 마가린에서 검출되었으며 1.10~14.53%의 범위였다. C18:2t의 총 함량은 2.02~7.91%의 범위로 다양하였으며, C18:3t는 0.33~l.39%의 수준이었다. 유지류의 trans 지방산 함량은 지방 100g당 5.40~16.558의 범위였으며, 대두유에서는 Cl8:1$\Delta$9t가 0.95%로 가장 높았으나, 옥수수유에서는 확인되지 않았으며, 쇼트닝은 3.1~5.1%로 1.6~4.3%인 라드보다 높은 수준이었다. 우유 및 유제품에서는 C16:1 $\Delta$9t, C18:1 $\Delta$9t, C18:2t 및 C18:3t가 골고루 분포되어 있었다. 양념류인 마요네즈에서는 C16:1$\Delta$9t가 확인되지 않았으나 4.93%의 trans 지방산이 검출되었다. 과자류 중 trans 지방산 함량이 현저하게 높은 팝콘(48%)에서 C16:l$\Delta$9t는 검출되지 않았으나 C18:1$\Delta$9t는 36%의 함량을 나타내었다. 빵류에서는 C18:1 $\Delta$9t가 trans 지방산 이성 체중 가장 높은 함량을 보였으며, 냉동피자의 C18:1 $\Delta$9t 함량은 30%를 초과하는 높은 함량을 보였다. 슈크림 빵은 높은 C18:2t 함량을 보였으며(8.26%), 불고기버거와 피쉬버거의 C18:2t trans 지방산 함량은 3.26%와 3.34%로 비슷한 수준이었다. 육가공품 및 튀김류 중 냉동 닭튀김과 냉동 감자튀김의 trans 지방산 함량이 15%~l9%의 수준으로 높았으며, C18:1$\Delta$9t과 C18:2t가 그 주된 성분이었다. 돼지고기구이 (목살)에서는 C18:1$\Delta$9t는 확인되지 않았으며, 냉동감자튀김에서는 C16:1 $\Delta$9t이 검출되지 않았다. 1회 분량 당 trans지방산 함량이 높은 식품은 팝콘, 냉동피자, 감자튀김과 닭튀김, 빵류 등의 순으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1363-1372
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• 2019

Objective: This study was designed to investigate the effect of diet supplementation with rubber seed oil and flaxseed oil on serum fatty acids profile, oxidation stability of serum and milk, and immune function of dairy cows. Methods: Forty-eight mid-lactation Holstein dairy cows were randomly assigned to one of four treatments for 8 wk, including basal diet (CON) or the basal diet supplemented with 4% rubber seed oil (RO), 4% flaxseed oil (FO) or 2% rubber seed oil plus 2% flaxseed oil (RFO) on a dry matter basis. Results: Compared with CON, all the oil groups increased the levels of trans-11 C18:1 (vaccenic acid), cis-9, trans-11 C18:2 (conjugated linoleic acid, CLA) and C18:3 (${\alpha}$-linolenic acid, ALA) in serum. Both the activity of glutathione peroxidase and catalase in serum and milk in oil groups were decreased, which were negatively correlated with the levels of cis-9, trans-11 CLA and ALA. The concentrations of proinflammatory factors (tumor necrosis factor ${\alpha}$ and interferon ${\gamma}$) in serum of oil groups were lower than that from the CON cows. Conclusion: These results indicate that diet supplementation with RO or FO could alter serum fatty acid profile and enhance the immune function of dairy cows. However, the negative effect on milk oxidation stability should be considered when feeding these n-3 polyunsaturated fatty acid-enriched oils in dairy production.

• 한국식품영양과학회지
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• 제29권5호
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• pp.957-964
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• 2000

부산지역 여고생의 1일 trans 지방산의 섭취 수준을 파악하기 위해 식사력조사와 식품섭취 빈도조사를 여름과 겨울 2회 실시하였다. 그 결과 간식빈도는 1일 2회가 38.0%로 가장 높았고, 간식시 선호하는 식품으로는 아이스크림이 46.4%로서 가장 높은 비율을 차지했으며, 비교적 우유와 유제품의 선호도가 높았다. 외식의 경우, 외식빈도는 1개월에 1번 외식한다고 응답한 사람이 가장 많았다(43.4%). 외식시 선호하는 삭품으로 돼지고기구이, 햄버거, 쇠고기구이가 각각 39.1%, 36 9%, 26.6%로서 높은 비율을 보였다. 야외에 패스트푸드는 여고생들이 선호하는 식품이며, 빵을 먹는 경우 마가린을 버터보다 더 선호하였다. Trans 지방산 섭취수준은 소득수준(p<0.05), 간식(p<0.001) 및 외식(p<0.05)의 빈도에 딸 유의적인 차이를 나타냈으며, 1일 평균 trans 지방산 섭취수준은 평균 $4.24{\pm}0.18g$이다. 우유와 유제품, 육류 및 가공품 등에 포함되어 있는 N-tFAs 성취수준은 086=0.00 g으로 1일 총 trans 지방산 섭취수준의 20.3%이며, H-tFAs 섭취 수준은 1일 총 trans 지방산 섭취수준의 79.7%에 해당하는 평균 $3.38{\pm}0.14\;g$이다. H-tFAs의 섭취수준이 N-tFAs의 수준보다 높았다(p=0.000). 본 연구의 결과, 우리나라 국민 전체의 trans 지방산 섭취수준은 외국에 비해 그다지 높지 않은것으로 추정되었으나 여고생들의 섭취 수준은 다른 계층에 비해 상당히 높은 편이다. 그리고, 현재의 식생활 패턴과 변화 추이를 볼 때 앞으로 trans 지방산 섭취수준은 상당히 높아질 것으로 예상된다. 따라서 가공식품들을 비롯한 각종 식품중의 trans 지방산 함량을 분석하여 기초 자료화하는 한편 여고생 뿐만 아니라 일반인들의 trans 지방산 섭취수준을 확립.제시해야 할 것으로 사료되어 이에 대한 연구가 계속 진행되어야 할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.189-194
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• 2013

The goal of this study was to evaluate the effects of lactation stage and individual performance on milk cis-9, trans-11 conjugated linoleic acid (CLA) content in dairy cows. In experiment 1, the milk cis-9, trans-11 CLA content from dairy cows in early ($0.33{\pm}0.014%$), middle ($0.37{\pm}0.010%$), and late stages ($0.44{\pm}0.020%$) showed significant differences (p<0.05); and the individual contents of the major fatty acids, especially cis-9, trans-11 CLA in cows of the same lactation were also variable. In the second experiment design as a validation test, our results once again showed that the individual contents of cis-9, trans-11 CLA were various, and a difference of about 2-fold (0.55% vs 0.95%) was observed, although the animals were offered same diet. These data demonstrated that lactation stage and individual performance have considerable effects on milk cis-9, trans-11 CLA contents.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1262-1270
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• 2008

The objective of this study was to investigate the effect of dietary supplementation with calcium salts of soybean oil fatty acids (CaSO) and linseed oil fatty acids (CaLO) on c9,t11-CLA production in ruminal fluid and milk fat from Holstein dairy cows. Rumen fermentation, lactational performances and fatty acid profiles in ruminal fluid and milk fat were also investigated. Twenty multiparous Holstein dairy cows were allotted randomly into two groups consisting of ten cows in each group according to calving date and average milk yield. The first group of cows was fed a control (without calcium salts) diet and a treatment as 1.0% of CaSO (on DM basis) for 30 days in each period. In the second group, cows were fed the same control diet and 1.0% of CaLO as a treatment in the same manner. The forage: concentrate ratio was 52:48, and diets were formulated to contain 17% crude protein (DM basis) for both groups. Ruminal pH, protozoal numbers and the concentration of total volatile fatty acids were unchanged, however, the ruminal ammonia-N decreased by feeding CaSO or CaLO treatment compared to the control diet. The vaccenic acid (trans-11 C18:1; VA) in rumen fluid increased (p<0.01) by 169% and 153%, and the c9,t11-CLA content of rumen fluid increased (p<0.01) by 214% and 210% in the CaSO and CaLO treatments, respectively, compared to the control diet. In milk fatty acids, the VA content increased by 130% and 132% in the evening and morning milking times, respectively, and the c9,t11-CLA content increased by 125% in both milking times for the CaSO supplementation than that of control diet. In the case of CaLO supplementation, the VA increased by 117% and 114%, and the c9,t11-CLA increased by 96% and 94% in the evening and morning milking times, respectively, compared to the control diet. The contents of VA and c9,t11-CLA of milk fatty acids were numerically higher in the evening milking time compared to the morning milking time for control and both treatments. Finally, these results indicated that the supplementation of CaSO or CaLO treatment increased the VA and the c9,t11-CLA in both ruminal fluid and milk fat of Holstein dairy cows.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.378-383
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• 2016

The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = $483{\pm}25.7kg$) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. $BW=486{\pm}27.5kg$) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p<0.05). The fatty acid composition (caprate, laurate, myristate, pentadecanoate, palmitate, palmitoleate, margarate, cis-11 heptadodecanoate, stearate, oleate, trans-vaccenate, linoleate, cis-11 eicosenoate, docosa hexaenoic acid, and docosa pentaenoic acid) of the beef showed no difference between the two groups (p>0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef.

• 한국식품조리과학회지
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• 제28권4호
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• pp.443-450
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• 2012

This study was investigated the effect of an oil-water fryer on quality properties of deep frying oil used for chicken. The acid and conjugated dienoic acid values of frying oil using an oil-water fryer were lower than those using a deep fat fryer, whereas the iodine value of frying oil using an oil-water fryer was higher than that using a deep fat fryer. The peroxide values of frying oil did not increase with frying number. Frying oil with an oil-water fryer had lower palmitic acid, stearic acid and oleic acid, and higher linoleic and linolenic acid contents than those with a deep fat fryer. The contents of trans-fatty acids in frying oil using a deep fat fryer and oil-water fryer after frying 110 chickens were 0.75% and 0.47%, respectively. The benzopyrene contents of frying oil using a deep fat fryer and an oil-water fryer after frying 110 chickens were 2.20 and $1.61{\mu}g/kg$, respectively.

• 한국식품영양과학회지
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• 제36권8호
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• pp.1010-1014
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• 2007

대전 및 충청 지역에서 판매되고 있는 즉석식품의 조지방 및 트랜스지방산의 함량을 조사하고자 찹쌀도넛과 꽈배기 각각 6종, 고구마 스틱 2종, 토스트 9종, 그리고 멀티플렉스 극장 팝콘 11종 등 총 41종을 수거하여 분석하였다. 조지방 함량은 멀티플렉스 극장 팝콘 13.74${\sim}$38.09%로 가장 높았으며, 고구마 스틱 20.82${\sim}$20.88%, 오징어 튀긴 9.37${\sim}$25.53%등의 순이었다. 수거한 시료는 유탕처리 식품이었으며, 멀티플렉스 팝콘은 전자렌지용 팝콘과 달리 트랜스지방산 함량이 매우 적게 분석된 것으로 보아 식물성 부분 경화 유지를 상요하지 않은 것으로 보인다. 트랜스지방산 함량은 대부분 1 g(g/100 g food) 미만으로 낮았으나, 멀티플렉스 극장 팝콘 중 한 시료만이 3.08 g(g/100 g food)으로 높게 나타났다.

• Natural Product Sciences
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• 제25권4호
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• pp.348-353
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• 2019

Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC₅₀ value of 3.4 μM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1425-1429
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• 2005

Megaspahera elsdenii YJ-4, which was previously isolated as a producer of trans-10, cis-12 CLA, was studied for its carbon source on the CLA production. M. elsdenii YJ-4, was incubated with glucose and lactose, and cultured in batch and continuous culture systems with linoleic acid at various pHs to investigate CLA production. Batch cultures of the ruminal bacterium, M. elsdenii YJ-4, were resistant to stearic acid and linoleic acid, and little growth inhibition was observed even when the fatty acid concentration in the culture was as much as 4 mg $ml^{-1}$. Stationary phase batch cultures (0.25 mg bacterial protein $ml^{-1}$) that had been grown on lactate and incubated with linoleic acid (0.20 mg $ml^{-1}$) produced approximately 12 ${\mu}g$ trans-10, cis-12 CLA mg $protein^{-1}$ and little cis-9, trans-11 CLA was detected. Some linoleic acid was converted to hydrogenated products (chiefly stearic acid), but these fatty acids were less than 5 ${\mu}g$ mg bacterial $protein^{-1}$. Stationary phase batch cultures that had been grown on glucose produced at least 3-fold less trans-10, cis-12 CLA than ones grown on lactate. Cells from lactate-limited continuous cultures produced less trans-10, cis-12 CLA than those from batch culture, but only if the pH was greater than 6.4. When the pH of the lactate-limited continuous cultures was lower than 6.4, trans-10, cis-12 CLA and hydrogenated products declined. Cells from glucose-limited continuous cultures produced less trans-10, cis-12 CLA and hydrogenated products than the cells that had been limited by lactate, but pH had little impact on this production. These results support the idea that M. elsdenii YJ-4 could be one of the major producers of trans-10, cis-12 CLA which causes cows to produce milk with a low fat content.