• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.6
• /
• pp.961-967
• /
• 2011

This study is about 'Ji-Qi-Shang-Chong(其氣上衝)' in Shang-han-lun("傷寒論")'s 15th text. Shang-han-lun is a basic text about pathology of Traditional Korean Medicine written by Zhang-Zhong-Jing(張仲景). In that text, there are so many cases of people having some symptoms, how to treat them, and which herb medicine to give them, and the side effects of wrong treatments. In those cases, there is symptom said 'Ji-Qi-Shang-Chong(其氣上衝)' in the 15th text. But there is no detailed description about that. So this study is aimed at studying exactly meaning of the 15th text's 'Ji-Qi-Shang-Chong(其氣上衝)' by comparing historical medical practitioners and analyzing with the bibliography, pathology, herb pharmacology, herbal medicine, pharmacology part. In the bibliographical analysis, this sentence has been transmitted from original Shan-han-lun written by Zhang-Zhong-Jing(張仲景). Former part of this sentence "太陽病, 下之後, 其氣上衝者, 可與桂枝湯". is most correspondent part with Zhong-Jing(仲景)'s. And there is correctional possibility about latter part.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.22 no.1
• /
• pp.82-88
• /
• 2008

We evaluated the potential protective activity of the traditional Korean medicinal herb, Corni fructus (CF), in an experimental model of hepatotoxicity induced by carbontetrachloride $(CCl_4)$. The CF exhibited a hepatoprotective activity against Chang cell. And The expression of cytochrome P450 2E1 (CYP2E1), measured by RT-PCR and western blot, was significantly decreased in the CF treated Chang cell. But $CCl_4$ and CF has no significant effect on 1A1 and 3A1 isoform of cytochrome P450. Based on these findings, it is suggested that hepatoprotective effects of CF possibly related to antioxidative effects and downregulation of CYP2E1 expression.

• The Journal of Korean Medical History
• /
• v.20 no.2
• /
• pp.93-97
• /
• 2007

In "Huangdineijing", detailed descriptions of the human physiology, pathology, causes of diseases, and symptoms can be found. The doctors who studied "Huangdineijing" interpreted "Huangdineijing" from many different points of view and such contents are being put to practical use even today. Some parts, however, are not translated clearly enough. Contents of the book of Fuqibing falls into this category. It cannot be explained why the description of Fuqibing is mentioned after Fuqibing. This study is an organization of the writer's opinion on Fuqibing.

• Journal of Korean Traditional Oncology
• /
• v.11 no.1
• /
• pp.105-118
• /
• 2006

Two of the essential processes required for metastasis are neoangiogenesis and tumor cell invasion of basement membranes (BM) and extracellular matrix (ECM). Recently, data showed that herbs removing blood stasis has an anti-angiogenic effects. Tonifying vital Qi and eliminating pathogenic factor was a basic modality in Oriental oncology. In this study, we investigated several Qi and Blood tonics for potent angiogenic inhibitors. Methanol extracts of samples inhibited the proliferation of ECV-304 at the concentration of 100 ${\mu}g/m{\ell}$. Zizyphi Fructus, Glycyrrhizae Radix, Angelicae Gigantis Radix decreased the gelatinolytic activity of MMP-9 from ECV-304, at the concentration of 100 ${\mu}g/m{\ell}$ in gelatin zymography. In in vitro invasion assay, herbs inhibited the invasion activity of ECV-304 by 53% of control (Ginseng Radix), 39% (Zizyphi Fructus), 36% (Angelicae Gigantis Radix), 25% (Glycyrrhizae Radix). Ginseng Radix inhibited the capillary-like tube formation of ECV-304 at the concentration of 160 ${\mu}g/m{\ell}$, Angelicae Gigantis Radix and Paeoniae Radix Alba inhibited at the concentration of 320 ${\mu}g/m{\ell}$. These results indicated that Ginseng Radix, Glycyrrhizae Radix, and Angelicae Gigantis Radix could be considered as potent angiogenic inhibitiors.

• Journal of Haehwa Medicine
• /
• v.23 no.1
• /
• pp.105-114
• /
• 2014

Objectives : In this study, the antioxidant activities of the 80% ethanol and hot water extracts of Euphorbia supina Rafinesque were investigated. Methods : We measured total phenol contents, flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The production of reactive oxygen species was measured in LPS-induced RAW 264.7 cells using flow cytometry system. Results : The content of phenol in the hot water extract was $65.529{\pm}0.462mg/g$ and $126.932{\pm}1.894mg/g$ in the 80% ethanol extract, and that of flavonoid in the hot water extract was $16.063{\pm}0.471mg/g$ and $29.159{\pm}1.963mg/g$ in the ethanol extract. The 80% ethanol extract also showed higher DPPH and ABTS radical scavenging activities ($90.8{\pm}1.0%$ and $92.5{\pm}0.7%$) than the hot water extract ($81.5{\pm}0.5%$ and $91.5{\pm}0.2%$). The production of reactive oxygen species(ROS) was reduced dose-dependently by 80% ethanol and hot water extract at concentration of 1, 10 and $100{\mu}g/m{\ell}$ of RAW 264.7 cells. Conclusion : According to these results, the 80% ethanol extract of Euphorbia supina Rafinesque has a good anti-oxidative effects than the hot water extract. Thus, the 80% ethanol extract of Euphorbia supina Rafinesque may serve as useful natural antioxidants.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.30 no.4
• /
• pp.236-241
• /
• 2016

The aim of the study is to evaluate immune-enhancing effects of Yeonsan Ogye. Various extract of Yeonsan Ogye (200 and 400 mg/kg/daily) was treated orally to Balb/c mice for 1 week, before acute inflammation was induced by LPS. After cytokine (IFN-γ, TNF-α, IL-6, and IL-1β) and immune cells (white blood cell, neutrophil, lymphocyte, and monocyte) level by serum and blood were counted. As a result, Oral treatment of Yeonsan Ogye extract to the Balb/c mice were significantly decreased cytokine level in serum, in comparison with control group. in addition, production of white blood cell and monocyte in blood was decreased and granulocyte was increased respectively, in comparison with control. Our results demonstrated that Yeonsan Ogye extracts seem to have significant immune-enhancing. Thus, Yeonsan ogye may be developed as a raw material for new health food and medicine to ease the symptoms related with inflammatory and immune.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.17 no.3
• /
• pp.771-776
• /
• 2003

The present study was conducted to evaluate the regulation mechanism of nitric oxide(NO) by water-extracted Cyperus rotundus (WCR) in RAW 264.7 macrophages. We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. WCR enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis by WCR, WCR alone had an effect on NO synthesis. It was found that the production of NO of RAW 264.7 cells stimulated with lipopolysaccharide (LPS) could be markedly inhibited by WCR. Inhibition of NO production was achieved by reducing inducible nitric oxide syntheses(iNOS) mRNA expression. The expression of IL-I gene by WCR was investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1 family(IL-1 α, IL-1β) expressions were induced by WCR. These finding suggested that regulation of NO production by WCR may be, at least in part, associated with the regulation of iNOS mRNA expression and IL-1 family gene expression.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.26 no.4
• /
• pp.446-454
• /
• 2012

In order to evaluate the efficacy of SHT against atopic dermatitis (AD), various immune related cytokines as well as histological comparison were performed in animal models, and the results are described. Clinical skin index of the SHT treated group decreased significantly in weeks 11 and 13, compared to the control group. Also, CD4+ immune cell ratio in the dorsal skin was significantly decreased to 69%, and both epidermal and dermal skin thickness was decreased. Serum IL-4, IL-5, IL-6, IL-13, and TNF-${\alpha}$, which are all important markers of inflammation, were decreased to 64%, 44%, 87%, 48%, and 45%, respectively. The expression of histamine, a chemical transmitter increasingly released during the progression of inflammation, was significantly decreased to 47%. The production of IgE immunoglobulin was significantly decreased to 16% compared to the control group. In conclusion, SHT pacifies the activation of T cells, leading to suppression of both Th2 cytokine overexpression and infiltration of immune cells into skin. As a result, relative thinning of both epidermis and dermis were observed. With the results obtained from in vitro studies, the immune modulatory effect of SHT in AD animal models was experimentally demonstrated. This study should provide solid information to construct EBM and for clinical practice.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.20 no.4
• /
• pp.896-901
• /
• 2006

Zingiberis rhizoma(ZB) has been used to treat a various condition and disease in traditional oriental medicine. The present study was conducted to evaluate the immunomodulatory effect of aqueous-extracted ZB(ZBE) on methotrexate (MTX)-induced immune suppression in mouse spleen cells. In spleen cell proliferation assay, ZBE enhanced mitogenic activity in mouse spleen cells. In RT-PCR, ZBE induced IL-2, IFNr and IL-6 cytokine gene expression in mouse spleen cells. In spite of MTX treatment, IL-2, IFNr and IL-6 gene expressions sustained in MTX treated spleen cells. CD45R/B220, pan B marker was slightly increased in ZBE treated mouse spleen cells. IL-6, B cell tropical cytokine, production was induced by ZBE-treated mouse spleen cells and IL-6 production was sustained on MTX-ZBE co-cultured cells. ZBE administration enhanced suNival of S-180 bearing mouse. These data indicate that ZBE has a protective effect of immune suppression caused by MTX, and ZBE may be enhance cellular and humoral function by regulate cytokine gene expression as well as the mitogenic effect on spleen cells.

• Journal of Haehwa Medicine
• /
• v.21 no.1
• /
• pp.23-31
• /
• 2012

Allopathic medicine has had a lot of limits though it has maintained the status of mainstream medicine, therefore these days there has been a growing interests in traditional medicine of East Asia. According to this trend, the integration between allopathic medicine and traditional medicine has become a hot issue and the need of mutual understanding on their physiology and pathology increases. However, understanding of traditional medical theory based on ying-yang, five phases, viscera and bowels is still difficult to be accepted to allopathic medical fields. Therefore, this research tried to investigate the similarity and differences of the structures between five viscera and organs. As a result, this research found that the investigation and understanding on viscera in traditional medicine or organs in allopathic medicine has in common in some degree. However, the integration and comparison between two medicines should be done cautiously because there still are the basal different points of understanding of human structure and function.