• 한국식품저장유통학회지
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• 제14권2호
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• pp.194-200
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• 2007

전통발효식품의 기능성 및 저장성을 증진시킬 목적으로 전통된장으로부터 효소활성 및 항균활성이 우수한 CH-14 균주를 분리하여 그 특성을 검토하였다. 분리한 CH-14 균주는 그람양성, catalase 양성, oxidase 음성, 내생포자 및 편모를 가지는 간균으로 세포의 크기는 $0.5-0.7{\times}3.5-4.2{\mu}m$이었다. 최종 분리균을 Bergey's Manual of Systematic Bacteriology 및 Bergey's Manual of Determinative Bacteriology와 API 50 CHL Kit에 의한 당 발효실험의 결과를 토대로, Bacillus subtilis로 밝혀져 Bacillus subtilis CH-14로 명명하였다. 최적배지의 조성을 검토한 결과, 탄수원은 2% cellobiose, 질소원은 yeast extract와 peptone을 각각 0.5%씩 혼합 첨가하는 것이 효과적이었다. 무기염은 0.05%의 $MgSO_4{\cdot}7H_2O$가 적당하였다. 최적 생육온도 범위는 $30^{\circ}C-45^{\circ}C$이었으며, 초기 pH 범위는 pH 4.5-10.0이었다. 배양시간에 따른 항균물질의 생산성을 검토한 결과, 배양 24시간이 최대의 활성을 나타내었다. 다양한 종류의 유해미생물에 대한 최소저해농도(MIC)를 검토한 결과, E. coli와 P. mirabilis에 대해서는 5 mg/mL, S. aureus, S. enteritidis, V. parahaemolyticus에 대해서는 10mg/mL를 나타내었다.

• 한국수산과학회지
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• 제43권2호
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• pp.117-124
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• 2010

To examine the effective use of seaweeds, sea tangle (Laminaria japonica) was extracted with water and the resultant extracts were fermented with Saccharomyces cerevisiae. Four strains of S. cerevisiae were cultured in aqueous extracts from sea tangle. S. cerevisiae SC-2, which was isolated from a traditional Korean fermented food (Meju), was selected for further study based on the results of a sensory evaluation. No significant differences in proximate compositions, such as moisture, crude protein, crude fat, and crude ash, of the sea tangle extracts before and after fermentation were observed. The reducing sugar decreased as the fermentation period increased, and the contents of some free amino acids were also affected by S. cerevisiae SC-2 fermentation. However, the content of glutamic acid, which is a major taste compound in sea tangle extract, was not affected by fermentation for up to 36 hr by the SC-2 strain. To determine the antioxidant activity of fermented sea tangle extract (fermented for 36 hr by SC-2 strain), the radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and nitric oxide were investigated and xanthin oxidase inhibition assay was performed. The antioxidant activity increased by 8 to 35%. The greatest enhancement of antioxidant activity was seen in the superoxide radical scavenging assay with $100\;{\mu}g/mL$ of raw and fermented sea tangle extract. The anti-inflammatory activity of fermented sea tangle extract was also enhanced. The fermented sea tangle extract showed 34.2% inhibitory activity against nitric oxide synthesis versus 11.9% for raw sea tangle extract at $100\;{\mu}g/mL$ concentration. These results suggest that fermented aqueous extracts from sea tangle are a useful resources.

• 한국식품영양학회지
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• 제33권5호
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• pp.493-504
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• 2020

Gastrodia elata Bl. (GE) is a traditional herbal medicine used for the treatment hypertension and cerebrovascular disease. The purpose of this study was to investigate the antioxidant effect and blood pressure control ability of the GABA containing fermented Gastrodia elata Blume. The fermentation strain provided by Dr. Gi in Seoul National University is L. brevis GABA100, excellent strain of GABA production, which is involved in the ability to degrade gastrodin glycosides and activation of brain function. The Gastrodia elata Blume fermented by Lactobacillus brevis GABA 100 showed antioxidant activity (total phenolic contents, DPPH radical and ABTS anion scavenging activities) than unfermented Gastrodia elata Blume. In the spontaneously hypertensive rats (SHR), the concentration of fermented Gastrodia elata Blume was administered at 500, 1,000, and 2,000 mg/kg for 8 weeks. Systolic blood pressure decreased statistically significantly (p<0.05), especially 2 weeks after feeding fermented Gastrodia elata Blume. Also, it showed a significant decrease in low, medium, and high concentrations of fermented Gastrodia elata Blume at 4 weeks and 8 weeks. These results indicated that Gastrodia elata Blume fermented by the excellent strain of GABA production L. brevis GABA100 shows the antioxidant function and the effect of suppressing the increase in blood pressure.

• 한국생활과학회지
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• 제8권2호
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• pp.373-385
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• 1999

The purpose of this study was to investigate the difference between generations in awareness and acceptance of the commercial Korean traditional foods. the survey was carried out through questionnaire and the subjects were 316 middle school students and 299 parents of the other students in the same schools. Five middle schools in Chongju, Chungbuk were included. The results were as follow: 1) Students showed higher awareness than adults for cooked rices and much lower for gruels except pumpkin gruel. Difference between generations was existed in the acceptance for Yuk-gae-jang rice, plain rice, abalone porridge, pollack gruel and pinenut gruel. 2) Among soups, students were more aware of seaweed soup and rib-broth than adults and adults were more aware of bone broth than students. Students showed higher acceptance than adults for seaweed soup, Yuk-gae-jang, Gom-tang and rib broth, whereas adults showed higher acceptance than students for pollack soup. 3) Most of one bowl meals were highly known by both students and adults without any specific generation differences. Students liked the best sauteed rice cake and they like sauteed rice cake and Japchae(sauteed noodle with mixed vegetables) much more than adults. 4) There was no generation difference in the awareness of grilled and pan fried foods Grilled and pan fried foods were favored more by students than adults except grilled laver which was highly favored by both groups. 5) Generation difference was appeared in the awareness of Kimchi and salt fermented fishes, especially salt fermented fishes were much more known by adults than students. And also the acceptance of adults were higher than that of students for the most Kimchi and salt fermented fishes.

• 한국식품저장유통학회지
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• 제8권1호
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• pp.92-101
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• 2001

전통 발효 식품인 가자미 식 해로부터 cholesterol oxidase 생산성이 있는 균주를 분리하고 이들 분리된 균주들로 부터 여러 단계의 균주 선별시험을 통하여 cholesterol oxidate 생산성이 우수한 미생물을 선별하여 그 특성을 조사하였다. Cholesterol oxidase의 생산성이 가장 우수한 SFF34의 배양학적, 생리학적 특성 및 세포벽 지방산 조성을 조사하여 Bergey's Mannual of Systematic Bacteriology의 분류기준에 따라 동정한 결과 Bacillus sp. 또는 그 유연균으로 동정되어 분리균을 Bacillus sp. SFF34로 명명하였다. Cholesterol oxidase의 생산 조건을 검토한 결과 최적 배지 조성은 2.0% glucose, 0.5% yeast extract, 0.03% MgSO$_4$.7$H_2O$, 0.02% $K_2$HPO$_4$, 0.2% NH$_4$NO$_3$, 0.2% Cholesterol로 판명되었으며, 배양 조건은 3$0^{\circ}C$, 초기 pH 7.0, 진탕속도는 150rpm에서 24시간 배양시효소 생성이 가장 우수하였다 (2.42 U/ml).

• 한국식품과학회지
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• 제28권1호
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• pp.152-156
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• 1996

우리나라 고유의 전통 조미식품인 고추장의 품질개선과 담금 방법을 과학적으로 표준화하기 위하여 전국의 각 가정에서 담은 55점의 전통 고추장을 수집하여 맛성분 특성을 조사하였다. 고추장의 유리당은 glucose $(8.21{\pm}5.62%)$와 maltose $(6.95{\pm}7.27%)$가 대부분이고 fructose $(1.88{\pm}1.27%)$와 sucrose $(1.05{\pm}1.21%)$가 소량 존재하였다. 유기산은 succinic acid $(901.83{\pm}826.23\;mg%)$, citric acid $(484.16{\pm}242.89\;mg%)$, lactic acid $(381.63{\pm}367.88\;mg%)$ 함량이 많았고 acetic acid, oxalic acid, formic acid는 소량 존재하였다. 고추장 중의 총 유리 아미노산은 64.35 mg%이었으며, proline $(10.66{\pm}6.27\;mg%)$, glutamic acid $(9.27{\pm}10.97\;mg%)$, aspartic acid $(9.14{\pm}5.84\;mg%)$, lysine $(6.19{\pm}6.66\;mg%)$, serine $(5.72{\pm}3.79\;mg%)$함량이 많았다. 핵산 관련물질은 CMP가 $42.90{\pm}28.16\;mg%$으로 가장 많았고, 다음으로 hypoxanthine $(6.86{\pm}3.45\;mg%)$, IMP $(5.59{\pm}5.84\;mg%)$, inosine $(4.58{\pm}6.91\;mg%)$, GMP $(3.36{\pm}3.93\;mg%)$순이었으며 AMP나 UMP는 소량 존재하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.39-39
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• 2015

Korean traditional Nuruk has been developed with various materials and shapes according to geographical environments and climates of their origins. Nuruk is also known as kokja in Korea, reflecting the understanding that microorganisms such as wild fungi, yeasts, and lactobacillus bacteria are naturally inoculated and reproduced. The objective of this study is to identify the characteristics of traditional Nuruk through recreating traditional production methods detailed in ancient Korean documents. In the present study, a total of 58 different kinds of Korean traditional Nuruk were prepared, including 46 kinds of recreated products. Each Nuruk sample was evaluated for its enzymatic activities, including glucoamylase, protease, and glucanase. Their suitability for alcoholic beverage production were compared to each other. To isolate valuable microorganisms from Nuruk samples, alcoholic beverages produced using each sample were subjected to sensory evaluation to determine their taste. In addition, metabolite changes in traditional alcoholic beverages fermented with different kinds of Nuruk were analyzed through mass-based metabolomics approach. This study presents, for the first time, the traditional production methods written in ancient Korean documents using workable production methods supported by modern technologies. In addition, this study analyzed the characteristics of reproduced Nuruk. It could be utilized as a basis for studying traditional Korean traditional alcoholic beverages and their valuable microorganisms.

• 대한본초학회지
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• 제23권1호
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• pp.1-8
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• 2008

Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.

• 한국식품저장유통학회지
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• 제9권3호
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• pp.315-320
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• 2002

전통메주의 과다건조로 인한 품질저하 및 유해 곰팡이 오염으로 인한 위생적 문제를 개선하기 위하여 백국균(Roligosporus)을 종국 제조에 이용하여 "캡슐형 메주"를 착안하고 그 품질특성을 조사하였다. R oligosporus을 0.5, 1, 2% (w/w) 비율로 첨가하여 캡슐형 메주와 종국을 넣지 않고 성형한 대조구 I,II형을 10~15$^{\circ}C$에서 3일간의 자연 건조하였다. 27일간 실외에서 발효한 대조구 I형이라 하고, 대조구 II형과 캡슐 타입 메주는 $25^{\circ}C$에서 7일간의 1차 발효(습도 80%)와 20일간의 실외 2차 발효(2.7$^{\circ}C$, 12월)를 실시하여 제조하여 이들의 품질특성을 상호, 비교하였다. 수분함량은 대조구 메주보다 캡슐형 메주에서 2.88~7.55%까지 높은 함량 차이를 보였다. pH값과 적정산도는 유의적인 차이가 없었다. 아미노태 질소는 대조구 I형(347.2 mg%)보다 캡슐형 메주군(800.80, 816.0, 901.60 mg%)은 대조구 I형보다 2.2~2.6배의 높은 발효율을 보였다. 환원당 함량은 캡슐형 메주군이 2.78~3.13%로 대조구 I형은 2.10%, 대조구 II형은 2.31%에 유사한 값을 나타냈다. 대조구 I형의 색차계 색도의 명도(L)값은 대조구 II형 및 캡슐형 메주보다 높았다. 메주보다 높았다.

• Mycobiology
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• 제42권4호
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• pp.361-367
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• 2014

Makgeolli, also known as Takju, is a non-filtered traditional Korean alcoholic beverage that contains various floating matter, including yeast cells, which contributes to its high physiological functionality. In the present study, we assessed the levels of ${\beta}$-glucan and glutathione in various yeast strains isolated from traditional Korean Nuruk and selected a ${\beta}$-glucan- and glutathione-rich yeast strain to add value to Makgeolli by enhancing its physiological functionality through increased levels of these compounds. Yeast ${\beta}$-glucan levels ranged from 6.26% to 32.69% (dry basis) and were strongly species-dependent. Dried Saccharomyces cerevisiae isolated from Nuruk contained $25.53{\mu}g/mg$ glutathione, $0.70{\mu}g/mg$ oxidized glutathione, and $11.69{\mu}g/g$ and $47.85{\mu}g/g$ spermidine and L-ornithine monohydrochloride, respectively. To produce functional Makgeolli, a ${\beta}$-glucan- and glutathione-rich yeast strain was selected in a screening analysis. Makgeolli fermented with the selected yeast strain contained higher ${\beta}$-glucan and glutathione levels than commercial Makgeolli. Using the selected yeast strain to produce Makgeolli with high ${\beta}$-glucan and glutathione content may enable the production of functional Makgeolli.