• 대한한의학방제학회지
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• 제19권2호
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• pp.1-10
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• 2011

Objectives : Since its enactment in 1987, the 56 standard prescriptions covered by insurance have remained unchanged from its original version. In this study, we tried to discover most frequently used herbal combinations by analyzing prescriptions used in actual clinical settings. Methods : We have built Structured Query Language to analyze herbal combination and progressed this analysis through analyzing the frequencies of medicinal herb combinations in medical prescription slips. Results : We have found out that traditional Korean medical doctors use about 13 herbs in a prescriptions and usually use 253 kinds of herb. And We have found out the most frequently used herbal combination. Conclusions : In this study, We can suggest new method to decide what do we need on insurance prescriptions.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.102-102
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• 2018

Protaetia Brevitarsis Seulensis(white grub) has been traditionally used as medicinal stuff to treat blood stasis, occlusion of menstruation, tetanus and liver cancer in Asian countries (Korea, Japan, China, Taiwan, India and Myanmar). Especially, Donguibogam, which is traditional korean medicinal book, described the white grub as traditional medicine to treat hepatic diseases and vascular disorders. The white grub has been considered as highly nutritional food. The major constituents of white grub are rich in protein, healthy fats, iron, calcium. Recent studies announced that white grub has hepatoprotective effect and anti-microbacterial effect. However, the immuno-enhancing effect of white grub extracts in RAW 264.7 macrophage cells has not been studied yet. In this study, the various concentrations of white grub extract were examined to find immuno-enhancing effects on RAW 264.7 cells. Cytotoxicity was determined by MTT assay and immuno-enhancing effect of white grub extract was investigated by measuring nitric oxide (NO) production compared with only lipopolysaccharide (LPS) treatment. White grub extracts (0.001 - 10 mg/ml) did not show cytotoxicity. Additionally, white grub extracts (0.001 - 1mg/ml) had Immuno-enhancing effect on RAW 264.7 cells compared with only LPS treated group. These results might be provided proof to develop beneficial immuno-enhancing material for human health.

• 대한본초학회지
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• 제27권3호
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• pp.39-55
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• 2012

Objectives : Recently there have been encouraging results, from a western perspective, in the cancer research field regarding the anticancer effects of herbal medicine. This paper was aimed to review herbal medicine playing its anticancer role in terms of apoptosis, inflammation control, differentiation and telomerase. Methods : New studies for tang, medicinal herb itself or effective ingradients of medicinal herb showing anti-cancer effectiveness were reviewed and summarized in terms of pharmacological action. Results : Ethanol extracts of $Spatholobus$ $suberectus$ greatly inhibited cancer cell growth inducing cell apoptosis and cytotoxic effects. $Scutellaria$ $baicalensis$ may be responsible for its anticancer activity showing inhibition of $PGE_2$ synthesis via suppression of COX-2 expression. Saikosaponins isolated from $Bupleurum$ induced the differentiation of C6 glioma cells, cancer cells, into astrocytes, normal cells. Acetone extract of $Bupleurum$ $scorzonerifolium$ inhibited proliferation of human lung cancer cells via inducing apoptosis and suppressing telomerase activity. Conclusions : Herbal medicine inhibited cancer cell growth inducing cell apoptosis and cytotoxic effects. Inflammation persisting for a decade eventually elevates the risk of cancer sufficiently that it is discernible in case control epidemiological studies. Differentiation therapy is defined as a therapy to treat cancers by inducing differentiation of the stem cells. Telomerase expression is a hallmark of cancer. Nearly the complete spectrum of human tumors has been shown to be telomerase positive.

• 한방안이비인후피부과학회지
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• 제35권2호
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• pp.28-48
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• 2022

Objectives : The purpose of this study is to investigate the trend of traditional Chinese medicine for sudden sensorineural hearing Loss(SSHL) in Chinese journals. Methods : Chinese National Knowledge Infrastructure(CNKI) and Wanfang med online were used to search randomized controlled trials(RCTs) on SSHL which were published from January, 2017 to December, 2021. Results : Among Chinese studies, Tonifying and Replenishing medicinal(補益藥) and Exterior-releasing medicinal(解表藥) were the most frequently used medicine in treating SSHL. Acori Graminei Rhizoma(石菖蒲) was the most frequently used herb excluding Glycyrrhizae Radix(甘草). All studies reported that using Traditional Chinese Medicine alone or combining western medicne(WM) is more effective for treating SSHL than using WM alone, although one study reported the result is not statistically significant. Conclusions : In analysis of selected studies, co-treatment of traditional chinese medicine and WM can be more effective way for treating SSHL than using WM alone. Side effects of treating SSHL can also be reduced by co-treatment of traditional chinese medicine and WM.

• 한국미생물·생명공학회지
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• 제33권3호
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• pp.236-239
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• 2005

Ethyl acetate extracts of traditional medicinal herbs were screened for their antifungal activity against Malassezia furfur through the liquid growth inhibition method with 96 well plate and the paper disk agar diffusion assay. Among the 91 kinds of herbal solvent extracts, borneolum (borneo-camphor, No. 56) was finally selected as the best antifungal effective medicinal herb. The MIC value of No. 56 against M. furfur was 0.3 mg/ml ($8.2{\times}10^6$ CFU/ml) and it was three times more efficient than midazole which is the known antifungal agent in clinical medicines.

• 고문화
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• 64호
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• pp.47-74
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• 2004

필자는 민속의약에 대한 연구가 일천한 현실에서 이에 대한 이해를 도울 목적으로 350년의 역사와 전통을 갖는 대구약령시(大邱藥令市)를 중심으로 전통의약과 관련한 유$\cdot$무형 전승문화의 존재양상을 유물과 자료, 지식과 기능, 행위전승 등의 측면으로 나누어 고찰해 보고자 하였다. 대구약령시의 유$\cdot$무형 전승문화는 약령시의 고유기능 수행과 관련하여 오래 전부터 전해져 내려온 유형적 문화요소와 기능$\cdot$지식$\cdot$행위양식$\cdot$규범 등의 무형적 은화요소를 총칭한다. 약령시 전승문화는 약령시 사람들의 생업활동과 관련된 물질전승과 기능 지식전승, 행위전승 등으로 대별된다. 물질전승은 한약재의 관리와 유통, 질병 치료를 포함하여 약령시 유지에 필요했던 각종의 물질적 증거들(material evidences)로서 약령시전시관과 한방 관련업소 등에 전시 및 소장되어 있다. 지식$\cdot$기능전승은 한약재를 정선(세척$\cdot$절단$\cdot$건조$\cdot$선별$\cdot$작근) 하여 저장$\cdot$유통시킴은 물론 약으로 제조하거나 치료하는 등의 관련 지식과 기능을 의미한다. 이는 대부분 업소에 오래 동안 종업원으로 근무하면서 형성되는 전인적 인간관계에 기초한 도제시스템에 의해 전승되어 榮다. 대구약령시에는 유구한 역사와 전통 및 전문 인력의 밀집과 수세대에 걸친 비전(秘傳) 등으로 많은 비방과 탁월한 약재 감별지식이 전승되고 있다. 약령시 행위전승은 약령시의 생활경험 및 문화사적 의의와 가치를 발양하고 계승하기 위해 반복되는 행위의 여러 표상들을 제의적$\cdot$기예적$\cdot$오락적 차원으로 정형화한 것으로서 약령시축제와 약령제가 대표적이다. 이상의 사실처럼, 대구약령시는 민속의약 전통에 대한 맥락적 이해를 가능하게 해주는 중요한 삶의 공간이자 민속의약의 저수지이기도 하다. 따라서 약령시 사람들의 생활경험과 기억을 포함하여 관련 물증들을 시$\cdot$공간적 차원에서 수집$\cdot$분류$\cdot$해석하고 효율적으로 보존$\cdot$계승$\cdot$발전시킬 수 있는 방안을 강구할 필요가 있다.

• Journal of Applied Biological Chemistry
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• 제57권2호
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• pp.113-122
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• 2014

A5E is complex of several medicinal herb ethanol extracts. The aim of this study is investigating the anticancer effect for non-small cell lung cancer. The antitumor effects of A5E on NCI-H460 were examined by regulation of cell proliferation, apoptosis, cell cycle arrest, mitochondrial membrane potential (${\Delta}{\Psi}_m$), and apoptosis-related protein. Cell proliferation was measured by MTS assay. Apoptosis induced by A5E was confirmed by Annexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) staining, and cell cycle arrest was measured by PI staining. NF-${\kappa}B$ translocation was detected by immunofluorescence and MMP (${\Delta}{\Psi}_m$) was measured by JC-1 staining. The expression of extrinsic pathway molecules such as FasL and FADD were elevated, and procaspase-8 was processed by A5E. In addition, intrinsic pathway related molecules were altered. The Bcl-2 and Bcl-xl levels decreased, Bax increased, and cytochrome C was released. In addition, the mitochondrial membrane potential collapsed, and caspase-3 and poly-(ADP-ribose) polymerase were processed by A5E. Moreover, A5E affected the cellular survival pathway involving phosphatidylinositol 3-kinase (PI3K)/Akt and NF-${\kappa}B$. PI3K and Akt were downregulated, also NF-${\kappa}B$ expression was decreased, and nuclear translocalization was inhibited by A5E. These results suggested that A5E delays proliferation, inhibit cell cycle progression and induce apoptosis in human lung cancer cell. We conclude that A5E is a potential anticancer agent for human lung carcinoma.

• Natural Product Sciences
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• 제26권1호
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• pp.1-9
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• 2020

Flavonoids are mainly contained in the vegetables and medicinal herbs. Until now, over 5,000 kinds of flavonoid have been identified and their biological activities have been reported. Among them, we are interested in oroxylin A and spinosin because of their specific structures having bulky group at C-6 of ring A. Oroxylin A is contained in the Scutellaria baicalensis and exhibits cognitive enhancing activity as a GABA_A receptor antagonist, which is different from those of mainly contained in the S. baicalenis, baicalein or wogonin. Spinosin is isolated from Zizyphus jujuba var. spinosa and mainly studied as a hypnotic or anxiolytic agent because of traditional knowledge about its original herb. As far as we know, the cognitive function of spinosin was first identified by our group. In this review, we discuss how such flavonoids exert their pharmacological activities associated with cognitive function based on the receptor binding study and behavioral studies. Traditional knowledge and reverse pharmacology may be addressed in the research field of phytochemical pharmacology and useful to unveil the secret of phytochemicals.

• 대한예방한의학회지
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• 제23권1호
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• pp.49-59
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• 2019

Objective : We searched and collected the various ways of taking medicine in Naegyeong, Dongeuibogam and studied the possibilities to apply the traditional ways of taking medicine to the current way of taking medicine. Method : We collected all the ways of taking medicine in Naegyeong and classified the ways according to the relative importance, urgency, pathosis, time of disease, and, the conditions of patients. Result : Medicinal forms are decoction, pills, trituration, and thin porridge. Various kinds of water and the prepared rice forms were used. A single herb was boiled and its water was used to take the medicine. Also, liquor and honey were used to take medicine. More than two herbs or special prescriptions were boiled and the extract water was taken. The same medicine was taken by different boiled water according to the condition and age of a patient, time, acute or chronic illness, and, severe or mild disease. Conclusion : There are a lot of pills and trituration prescriptions in Naegyeong, Dongeuibogam. Water, various rice preparations, and several herbs are used to take these prescriptions. The reason is that these ways of taking medicine promote the medicinal effect and fast treatment to maximize the medicinal effects. From now on, the in-depth and mutilple studies are needed based on this research.

• Applied Biological Chemistry
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• 제37권3호
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• pp.175-181
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• 1994

사삼, 길경, 작약, 당귀, 천궁, 황기 등 6종의 약초를 이용하여 전래의 방법으로 약용주를 제조하고 발효기간 중 총산, 환원당, 전당, pH, 품온, 알코올함량을 측정하였다. 발효기간은 초기, 중기, 말기로 뚜렷하게 구분되었다. 초기는 $1{\sim}4$일간으로서 모든 변화가 급격히 일어났고, 중기는 $5{\sim}7$일로 모든 변화가 완만하였으며, 말기는 $8{\sim}10$일로 거의 변화가 없었다. 총산은 발효초기에는 급격히 증가하였으나 후기에는 거의 변화가 없었다. 환원당, 전당 및 pH는 모든 시험구에서 발효초기에 급격히 감소하다가 후기에는 완만하게 감소하였다. 약용주의 알코올함량은 전기간에 걸쳐서 일반발효주보다 $0.3{\sim}1.5%$ 정도 낮았으며, 발효 종료일에 사삼주, 길경주, 작약주, 당귀주, 황기주, 천궁주 및 발효주의 알코올 함량은 각각 12.6, 12.9, 12.3, 12.4, 13.4, 12.6 및 13.6%를 나타내었다. 이러한 처리구별 차이는 원료, 제조방법, 약초양과 약초의 종류에 따른 것으로 생각된다. 관능검사 결과 전체적인 품질은 황기주가 가장 우수했고, 그 다음으로 천궁주, 사삼주, 길경주, 당귀주, 작약주 순으로 나타났다.