• KSBB Journal
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• 제25권4호
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• pp.401-407
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• 2010

We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).

• 한국식품영양학회지
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• 제11권1호
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• pp.20-25
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• 1998

건조과정 중의 amine의 함량 변화는 초기건조 온도의 상승으로 DMA와 TMA의 함량이 건조 9일째까지 급격히 증가하였고, 인공건조의 경우가 자연건조보다 DMA, TMA 함량이 낮게 나타났다. TMAO의 경우, 건조과정 중 분해되어 함량이 감소되어 DMA, TMA 함량과 역상관계를 보였다. DMA와 TMA의 생성은 건조과정에서 생성되고 있으며 유통과정에서 더욱 많이 발생된 것으로 사료되므로 유통에 따른 유해성 문제점을 심각하다고 판정되므로 유통방법 개선이 필요하다고 생각된다.

• 한국식품과학회지
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• 제1권1호
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• pp.78-84
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• 1969

An attempt has been made to investigate the possible occurrence of aflatoxins, a group of micotoxin which are toxic metabolites produced by Aspergillus flavus, and aflatoxin like substances in fungal fermented soybean products such as meju and soybean paste. Chloroform extracts from 15 samples which has been defated with petroleum ether are subjected to separations with thin-layer chromatography on silica gel washed with methanol prior to film coating, improves the chromatographic separation and ultraviolet absorption spectral identification. In addition to fluorescencing spot having an Rf value which is same as aflatoxin $B_1$, many of the fluorescencing spots have been occurred in every sample examined. Each fluorescencing spot is scratched and the ethanol extracts are subjected to further separation with thin-layer chromatography. Each fluorescence substance is eluted with ultra violet transparent absolute ethanol and the ultraviolet absorption spectra are checked. None of the absorption curve of eluates shsow accordance with the curve of aflatoxin. 2, 4-Dinitrophenyl hydrazine reagent, Tollen's reagent and ninhydrin reagent are applied on the chromatogram- The data show that aflatoxin is not present in any of the fungal preparations examined.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 추계학술대회 논문집 광주전남지부
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• pp.91-95
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• 2006

In whole world consciousness of environment maintenance have increased very quickly for the end of the 20th century. To use and disuse toxic substances have been controled at the field of industry. Also the field of lighting source belong to environmental control. And in the future the control will be strong. In radiational mechanism of fluorescence lamp mercury is the worst environmental problem. In radiational mechanism of fluorescence lamp mercury is the worst environmental problem root. In the mercury free lighting source system the Xe gas lamp is one type. And the Ne:Xe mixing gas lamp improvements firing voltage of Xe gas lamp. Purpose and subject of this study are understand, efficiency, ideal of Ne:Xe plasma which mercury free lamp. Before ICP was designed, basic parameters of plasma, which are electron temperature and electron density, were measured and calculated by langmuir probe data. Property of electron temperature and electron density were confirmed by changing ratio of Ne:Xe.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1994년도 교수 연수회(환경)
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• pp.405-411
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• 1994

Hazardous wastes released into the general environment are of concern to the public and to public health authorities. In response to this concern, the Comprehensive Environmental Response, Compensation, and Liability Act, as amended (commonly called Superfund), was enacted in 1980 to provide a framework for environmental, public health, and legal actions concerning uncontrolled releases of hazardous substances. The Agency for Toxic Substances and Disease Registry (ATSDR) was created by Superfund to address the public health issues of hazardous wastes in the community environment. Two key Agency programs, Public Health Assessments and Toxicological Profiles, are designed to assess the risk to human health of exposures to hazardous substances that migrate from waste sites or through emergency releases (e.g., chemical spills). The Agency's public health assessment is a structured process that permits ATSDR to identify which waste sites or other point sources require traditional public health actions (e.g.. human exposure studies, health studies, registries, health surveillance, health advisories). The ATSDR qualitative public health assessment complements the U.S. Environmental Protection Agency's quantitative risk assessment. For Superfund purposes, both assessments are sitespecific. ATSDR's toxicological profiles are prepared for priority hazardous substances found most frequently at Superfund sites. Each profile presents the current toxicologic and human health effects information about the substance being profiled. Each profile also contains Minimal Risk Levels (MRLs), a type of risk assessment value. This paper covers ATSDR's experience in conducting public health assessments and developing MRLs, and it relates this experience to recommendations on how to improve chemical risk assessments.

• 환경위생공학
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• 제6권1호
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• pp.7-25
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• 1991

Until now, pure drinking water grnerally menas the water without taste, odor, general bacteria, coliform, and other exotic substance. Such a definition has been changing recently due to the finding of numerous other inorganic and organic substances unknown to us. 10 years ago, major causes of death were infectious agents and parasites contained in water, but recently, it has become apparent that some substances contained in drinking water cause cancer and heart diseases. We must drink about 2L of water everyday in order to maintain healthy condition. Waters used for drinking include tap water, well water, spring water, filtered water, etc., but the quality of drinking water has more polluted due to the industrial development and population increase. For example, industrial waste waters from industrial plants pollute the water supply sources ; toxic substances contained in the waste waters pollute the ground water sources by penetrating the geological strata, and municipal, livestock, public building waste waters also pollute the water supply sources. Sometimes, the polluted surface waters were announced to be polluted by various kinds of orgainc substance, and it is reported that the pollution of ground water by orga nic substances has few in number but high in its concectration comparing with those of surface water. As the water quality pollution level increases, so the amount of disinfectant also increase. For example, chlorine solution, one of widely used disinfectants, creates trihalomethane(THM), a carcinogen, and halogen compounds. According to Oliver, through chlorine disinfection process, humine substance and chlorine create bolatile organic halide and nonvolatile organic halide by chemical reaction. There are tens or hundreds filtering devices, but filtering principles and maintenance metjhods are different, so their efficiency tests are needed. According to Smith, the effeciency tests aginst over 30 Ameican filtering devices show that 10 devices can remove 85% of volatile organics and further studies on filtered waters are underway. In consideration of important impacts of polluted drinking water on national health, authors studied the state of water quality pollution against tap water used as drinking water, filtration device passed water, ground water, and conserved drinking water ; tested the efficiency of filtration devices for tap water ; tried to sep up the detection method by using ion chromatography based on negative ion and positive ion by using single column, and attemped the simple filtration method for general households.

• 한국균학회지
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• 제14권1호
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• pp.61-70
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• 1986

Sterigmatocystin bears a close structural relationship to aflatoxin $B_1$ and is a carcinogenic compound that has been shown to affect various species of experimental animals. Reaction and toxicity of sterigmatocystin in the artificial gastric juice were investigated. Sterigmatocystin was degraded in artificial gastric juice and extracted by the method of A.O.A.C. After cleaned up by silica gel column chromatography, this substance was detected and characterized by thin layer chromatography, UV, IR and mass spectra. It showed $R{\mathcal{f}}$ 0.4 and brick-red color by TLC. Especially, in the mass spectrum of it, fragment peak at m/e 327 was due to the loss of the $-CH_3$ and $-H_2O$, fragment peak at m/e 341 was due to the loss of the $H_2O$ and $-H^+$, and fragment peak at m/e 239 was due to the loss of the 2-chloro-tetrahydrofuran and methyl group from the parent molecule. Therefore, a degraded substance of sterigmatocystin reacted in artificial gastric juice (Sub. K) was estimated with additional formation of hydrochloric acid. In four-day-old chicken embryos, the mean lethal dose $(LD_{50})$ was $140\;{\mu}g/egg$, and 90 to 100% of the embryos were killed with 1 mg/egg. This $LD_{50}$ $140\;{\mu}g/egg$ compared with an $LD_{50}$ $14.69\;{\mu}g/egg$ for sterigmatocystin (acute toxicity) showed the substance to be much less toxic than sterigmatocystin.

• 한국환경농학회지
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• 제33권4호
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• pp.314-320
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• 2014

BACKGROUND: Run off from agricultural sites contaminates water bodies with nitrogen which is toxic and causes eutrophication when excessively accumulated. Hence, the interest in monitoring nitrogen toxicity in aquatic environment has been continuously increasing. METHODS AND RESULTS: To detect a real time toxicity of various nitrogen compounds, we applied biomonitoring method (biosensor) based on sulfur-oxidizing bacteria (SOB). The toxicity biomonitoring test was conducted in semi-continuous mode in a reactor filled with sulfur particles (2~4 mm diameter) under aerobic condition. Relative toxicity was simply determined by measuring the change in electrical conductivity (EC). Various nitrogenous compounds at different concentrations were evaluated as a potential toxic substance. Nitrite was found to be very toxic to SOB with a 90% inhibition even when the concentration as low as 3 mg/L. However, nitrate and ammonia have any inhibitory effect on SOB's activity. CONCLUSION: The biosensor based on SOB responded sensitively to nitrite even at substantially low concentrations. Therefore, it can be used as a reliable biological alarm system for rapid detection of contaminants due to its simplicity and sensitive nature.

• 한국산업보건학회지
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• 제21권2호
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• pp.82-89
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• 2011

Methylcyclohexane is frequently used in industrial sites (2,592tons/year) as rubber adhesives, ink, paint thinners, organic solvents, and so on. However, there are limited data on the toxic evaluation of methylcyclohexane. This study aims to predict the hazards and neurological effects of methylcyclohexane using SD rats in order to prevent health disorders of workers. The OECD Guideline for Testing of Chemicals (OECD, 2001) was used as a reference during the tests. For 13 weeks (once a day, five days per week) 0, 10, 100 and 1,000mg/kg/day of methylcyclohexane was injected to SD rats to observe any changes in the body or organ weight, hematology, histopathology, mobility, blood pressure, and neurotransmitter. As a result, some male and female SD rats injected with 1,000mg/kg/day of methylcyclohexane died. On the other hand, surviving rats showed significant changes such as hematological changes involving the decrease in the number of red blood corpuscles, and the decrease or increase in the weight of the lungs, kidneys, spleens, and livers (p< 0.05, p<0.01). Also histopathological lesions were observed in the hearts and kidneys. In the test for the effect on the nervous system, SD rats injected with 100mg/kg/day of methylcyclohexane had higher blood pressure levels compared to the control group. However, no abnormal effects was observed in the mobility, serotonin, neurotransmitter, and the biopsy of the brain and coronary arteries. The study results revealed that the livers, hearts, and kidneys were affected by methylcyclohexane. The absolute toxic dose of methylcyclohexane is 1,000mg/kg/day, NOAEL is 100 mg/kg/day, and it is not a toxic substance to the nervous system.

• 생약학회지
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• 제50권3호
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• pp.205-218
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• 2019

This study was conducted to investigate the toxicity symptoms and approximate lethal dose (ALD) of Oplopanax elatus (Nakai) Nakai hydrothermal extract powder by single oral dose toxicity and 4 weeks of repeated oral dose determination. The Sprague-Dawley (SD) male and female rats were treated with 1,250 (low- dosage group), 2,500 (medium- dosage group) and 5,000 (high- dosage group) mg/kg. In the single oral dose toxicity test, no dead animals and toxic symptoms were observed during the experiment. And there were no related with anomalies in normal weight changes and autopsy results. In the four-week repeated oral dose determination test, no death animals and toxicity symptoms were observed during the experiment, and there were no abnormal results in weight changes, feed and negative intake measurements. Results of eye examination, urinalysis, hematological values and serum biochemical values, gross findings and absolute organ were not of singularity. These result demonstrated that no toxic symptoms were observed by the test substance Oplopanax elatus (Nakai) Nakai hydrothermal extract powder under this test condition, and the non-toxic content is determined to be 5,000 mg/kg/day.