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Selection of Inhibitor-resistance Yeast and its Application to Bioethanol Production in the Hydrolysate of Rape Stem  

Yeon, Ji-Hyeon (Department of Biotechnology, Chungju National University)
Kim, Hye-Ji (Department of Biotechnology, Chungju National University)
Oh, Sung-Ho (Division of Biomaterials Engineering, Kangwon National University)
Lee, Hyeon-Yong (Division of Biomaterials Engineering, Kangwon National University)
Jung, Kyung-Hwan (Department of Biotechnology, Chungju National University)
Publication Information
KSBB Journal / v.25, no.4, 2010 , pp. 401-407 More about this Journal
Abstract
We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).
Keywords
hydrolysate of rape stem; bioethanol; inhibitor cocktail; Sacchromyces cerevisiae; Pichia stipitis;
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Times Cited By KSCI : 4  (Citation Analysis)
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