KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Selection of Inhibitor-resistance Yeast and its Application to Bioethanol Production in the Hydrolysate of Rape Stem

유채대 가수분해물에서 inhibitor 저항성 효모선별과 이를 이용한 bioethanol 생산

  • Yeon, Ji-Hyeon (Department of Biotechnology, Chungju National University) ;
  • Kim, Hye-Ji (Department of Biotechnology, Chungju National University) ;
  • Oh, Sung-Ho (Division of Biomaterials Engineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (Division of Biomaterials Engineering, Kangwon National University) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Chungju National University)
  • 연지현 (충주대학교 바이오산업학과) ;
  • 김혜지 (충주대학교 바이오산업학과) ;
  • 오성호 (강원대학교 생물소재공학) ;
  • 이현용 (강원대학교 생물소재공학) ;
  • 정경환 (충주대학교 바이오산업학과)
  • Received : 2010.06.01
  • Accepted : 2010.08.23
  • Published : 2010.08.31
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Abstract

We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).

Keywords

References

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