• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.117-120
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• 2000

본 연구는 4가지 종류의 재조합 발광성 미생물을 이용하여 내분비계 장애물질로 알려진 여러 가지 물질에 대한 cellular toxicity를 유전자 손상, 단백질 손상, 산화적 손상, 생물막 손상으로 구별하여 확인하였다. 4가지 발광성 미생물의 반응성에 따라 내분비계 장애물질의 독성 형태를 규명할 수 있었고, 생명체에 미치는 독성 정도를 확인할 수 있었다. 또한 유전자 손상을 탐지할 수 있는 DPD2794의 경우 유전자 손상을 일으키는 형태에 따라 두 그룹으로 보다 세분화가 가능하였다. 따라서 본 연구결과를 바탕으로 내분비계 장애물질이 호르몬 교란으로 인한 피해뿐만 아니라 cellular toxicity로 인한 피해 역시 입힐 수 있는 것으로 확인하였고, 이들 발광성 박테리아를 이용하여 그 독성 형태를 정확하게 파악할 수 없었던, 유해 물질들의 분류를 위한 screening method로의 개발 역시 가능할 것이다.

• 한국응용곤충학회지
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• 제28권1호
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• pp.32-36
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• 1989

Cypermethrin과 pirimicarb도태 저항성계통 복수아흑진딧물(Myzus persicae Sulzer)에 대한 acephate, cypermethrin, demeton-S-methyl, pirimicarb 상호 혼합시의 연합독작용을 검토한 결과는 다음과 같다. Cypermethrin 도태 저항성계통에 Cypermethrin+acephate, cypermethrin+demeton-S-methyl, cypermethrin_pirimicarb를 처리하였을 때, 혼합비에 따른 정도의 차이는 있었으나. 독립작용 또는 길항작용을 보였다. Pirimicarb 도태 저항성계통에 pirimicarb+acephate, pirimicarb+cypermethrin을 각각 8:2, 2:8 혼합처리하였을 때 최대의 협동결과를 보였으나, pirimicarb+demeton-S-methyl의 경웨는 혼합비에 관계없이 길항작용을 나타내었다.

• 한국초지조사료학회지
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• 제32권4호
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• pp.325-334
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• 2012

It has been known that endophyte (Neotyphodium coenophialum) is beneficial to tall fescue (Schedonorus phoenix Scop.) because the mutualistic endophyte is able to confers tolerance against abiotic and biotic stresses to tall fescue. However, this fungal endophyte produces toxic alkaloid resulting in negative effects on animal performance. Recently, Non-toxic endophyte have been developed and inserted into tall fescue to avoid detrimental effect on animal but remaining positive influence on tall fescue. In order to keep this beneficial impact, it is essential to have endophyte infected tall fescue through vertical transmission from maternal plants to seeds. Little research has been carried out on endophyte transmission. To get basic information related to endophyte transmission, experiment was conducted to examine the effect of plant genotype on endophyte transmission under water stresses. Overall endophyte concentration in seeds was higher than that in panicles and endophyte concentration in seeds and panicles relied on plant. This study revealed that drought is not a critical component to control the endophyte transmission from maternal plants to seeds. Plant genotype is an important factor controlling the endophyte transmission from plant to seed.

• 한국물환경학회지
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• 제22권1호
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• pp.145-149
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• 2006

Textile and pigment wastewater samples collected from an industrial complex showed toxic effect on Daphnia magna. For textile wastewater, 48 h acute toxicity of effluent was not detected while toxic unit (TU) of influent was 1.79. The toxicity of influent was completely disappeared by gamma-ray treatment at 10 kGy or by suspended solids (SS) removal. In case of pigment wastewater, both influent and effluent were toxic to D. magna though the effluent satisfied current water quality standards. Gamma-ray treatment had little effect on the toxicity reduction of pigment wastewater since the toxicity was mainly caused by metal ions, in particular, Cr(VI). This work suggests the bioassay technique for monitoring adverse effects of wastewater should be introduced, and also shows the usefulness of gamma-rays as an advanced treatment technique for textile wastewater.

• 대한의생명과학회지
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• 제16권2호
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• pp.96-104
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• 2010

In this study, the effects of HP4060, a pomegranate extract, on Sprague-Dawley (SD) female rats were investigated. SD rats used in the experiment were divided into 3 groups: a control group, 100 mg HP4060/kg rat powder fed group, and 25 mg HP4060/kg rat liquid fed group. After 20 days of administration, the changes of the brain neurotransmitters were measured. The data showed that the concentration of the serotonin and the norepinephrine were increased, whereas that of the epinephrine was decreased in HP4060 administered groups. In addition, the improving effect of HP4060 on depression symptom of menopause women were shown by increased immobility time of the SD rates in a separate experiment. The uterus weight of HP4060 fed groups were also shown to be increased. In order to monitor toxic effect of HP4060, glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) levels were measured, and the results showed that no significant difference in GOT and GPT levels among experimental groups implying no significant toxic side effects of HP4060. According to these results, it seems clear that HP4060 may improve symptoms of hot flush and depression caused by menopause without significant level of toxic effects.

• 한국환경과학회지
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• 제4권2호
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• pp.201-214
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• 1995

Design approach of upflow Anaerobic Sludge Blanket(UASB) process based on the biological kinetic parameters are known to be very difficult since the characteristics of the granular slut비e depends on the type of wastewater and size distribution of the granular sludge also depends on the upflow velocity in the UASB reactors. Furthermore, industrial wastewater containing toxic substances has been treated by UASB process without the clear knowledge of toxic effects on the granular slut형e. Hence, the present research was aimed on the intensive evaluation of biological kinetic parameters of the granular sludge in UASB reactor with and without toxic substance of 2, 4-dichlorophenol in order to present the basic design measures for UASB process design. The results could be summarized as follows. The biological kinetic parameters(k and Ks) considerably varied with the granular size of the sludge. Generally, 연e k and ks values of the granular sludge increased with the particle size of the granule. The biological kinetic parameters(k and Ks) of the granular sludge obtained from batch test were not applicable to design purpose of UASB process due to substrate diffusional limitation into the granular sludge in the completely mixed UASB reactors. The toxic effects on k and Ks greatly varied with the granular sixte. And as the toxicant concentration increased, the k value decreased while the Ks value increased. Inhibition constant(hi) for k with the toxicant of 2, 4-dichlorophenol varied from 0.5 to 2.3 depending on the granular sizes while the inhibition constant(Ki) for Ks varied from 20.7 to 80.1, showing the mixed inhibition.

• Animal cells and systems
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• 제5권2호
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• pp.139-143
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• 2001

Alzheimer's disease is the most common form of dementia and no cure is known so far. Extensive genetic works and in vitro experiments combined with clinical observations link amyloid $\beta$--protein (A$\beta$-) to the pathogenesis of Alzheimer's disease (AD). It was hypothesized that $A\beta$- becomes toxic when it adopts a fibrillar conformation. Recently, non-fibrillar form of $A\beta$- was observed and the potential role in the pathogenesis of AD became an interesting subject. In this study, the cytotoxicity of non-fibrillar $A\beta$- and fibrillar $A\beta$- was compared on oxidative stress, membrane damage, or nucleosome break down. Non-fibrillar $A\beta$- was not toxic in peripheral nervous system-derived cells but significantly toxic in central nervous system-derived cells while fibrillar $A\beta$- was non-selectively toxic in both cell culture. The neurotoxicity of non-fibrillar $A\beta$- was reproduced in semi-in vivo culture of mouse brain slice. In conclusion, non-fibrillar $A\beta$- could be more relevant to the selective neurodegeneration in Alzheimer's brains than fibrillar $A\beta$- and further research needs to be done for identification of the cause of AD.

• 한국화재소방학회논문지
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• 제20권2호
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• pp.1-7
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• 2006

본 연구는 파스콘 트로프의 화재시 발생되는 연기의 유독성 평가를 위해 KS F 2271에 의한 연기유해성 시험 및 NES 713방법에 의해 연소가스를 분석하고, 독성지수를 확인해 봄으로서 연소시 인체에 미치는 영향을 간접적으로 확인해 보고자 한다. 실험결과 파스콘 트로프는 KS F 2271인 건축물의 내장재료 및 구조의 난연성 시험기준에 적합한 것을 확인하였다. NES 713 방법에 의한 파스콘 제품에서 발생하는 연소가스는 이산화탄소와 일산화탄소가 전부임을 알 수 있으며, 연소가스의 독성지수는 0.944로 나타났다. 이러한 수치는 제품의 연소시 발생되는 연소가스를 30분 이상 노출되어야 위험한 농도에 이룰 수 있다는 것을 의미한다. 난연성능 요구시 연기유독성 평가나 연기의 발생량을 규제하지 않고 있는 국내의 실정에 비추어 볼 때 파스콘 제품의 연소가스 독성지수는 비교적 낮은 수치이다.

• Archives of Pharmacal Research
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• 제8권4호
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• pp.267-272
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• 1985

Pyrantal pamoate's anthelmintic activity is due to its action as a neuromuscular blocking agent. It is generally well tolerated. Transient rises in SGOT levels have been reported in the drug-treated patients. Decreased levels of serum alkaline phosphatase post treatment were found in yound dogs. The present study was performed to investigate the possible toxic effects of pyrantal pamoate in pregnant mice progenies. The drug was given in different doses to these mothers in the first, second and third trimester. Serum alkaline phosphatase, SGOT and SGPT of one or two month old offspring were monitored. SGOT levels showed an increase in some doses in one and two month old offspring where alkaline phosphatase showed a decrease in some doses in one and two month old offspring. The latter effect may be due to osteoblastic alkaline phosphatase inhibition. The effect on SGOT levels, however, was difficult to explain, but may be due to a toxic effect on liver cells or cardiac muscles.

• 한국물환경학회지
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• 제23권5호
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• pp.766-775
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• 2007

The use of toxic chemicals was extended as the industry in Korea has grown dramatically during the last three decades. However, list of toxic substances and limit concentrations in the water environment are not consistent within management of ambient water, drinking water and industrial effluent. This article suggests the systematic framework to classify toxic substances in the water environment and deriving their effluent limits. The most important factor for decision-making to classify toxic substances is whether their concentrations in the water environment are higher than the reference concentrations, estimated by considering human health risk and ecological risk. Using a risk-based reference concentration, the ambient water quality criterion, it is possible to derive the regulatory limit concentrations of toxic substances in drinking water and in industrial effluent. The goal concentrations in the effluent, which guarantee the human and ecological safety, should be determined with scientific investigation, balancing environmental benefit and economical effect, considering availability of treatment technology and identifying characteristics of wastewater from different industries.