• 제목/요약/키워드: total viable count

검색결과 196건 처리시간 0.021초

치즈를 첨가한 김치의 발효 특성 (Fermentation Characteristics of Kimchi Supplemented with Cheese.)

  • 배인휴;최성희;최희영
    • 한국미생물·생명공학회지
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    • 제30권4호
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    • pp.415-419
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    • 2002
  • 본 연구는 배추김치 제조시 김치의 단백질원인 젓갈 대신 치즈를 첨가하였을 때 김치의 발효적, 이화학적 및 관능적 품질 특성을 조사하였다. 젓갈이 첨가된 대조구와 치즈가 첨가된 김치 모두 담근 후 2일까지 빠른 속도로 미생물이 증식되었으나, 담근 후 3일부터는 치즈 첨가 김치가 대조군 김치에 비하여 높은 생균수를 보였으며, 치즈 첨가량이 많을수록 더욱 더 높았다 pH는 대조구와 치즈 첨가 김치 모두 담근 후 하루동안 일시적으로 약간 상승하다가 2일째부터 급격히 저하되어 3일째에는 pH4.33-4.18로 가장 맛있다고 일반적으로 평가되는 김치의 pH 범위를 보였으며, 치즈 첨가량이 많을수록 pH가 다소 낮은 경향이었다. 발효속도는 대체로 치즈 첨가량이 많을수록 빨랐으며 특히 발효 3일 이후에는 7% 치즈 첨가군이 발효속도가 가장 빨랐다. 일반성분을 분석한 결과 치즈를 첨가한 시험구에서는 수분이 대조구보다 다소 낮게 나타났고 조단백질과 조지방이 다소 높은 경향을 보였다. 담근 후 3일 된 김치의 관능검사를 실시한 결과 젓갈을 넣은 대조구 김치보다 치즈를 3%, 5% 첨가한 김치가 외관, 향미, 질감, 맛 등 관능에서 대체적으로 좋은 점수를 받았다

Effect of Swiss Chard (Beta vulgaris var. cicla) as Nitrite Replacement on Color Stability and Shelf-Life of Cooked Pork Patties during Refrigerated Storage

  • Shin, Dong-Min;Hwang, Ko-Eun;Lee, Cheol-Won;Kim, Tae-Kyung;Park, Yoo-Sun;Han, Sung Gu
    • 한국축산식품학회지
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    • 제37권3호
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    • pp.418-428
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    • 2017
  • In this study, the effects of pre-converted nitrite from Swiss chard powder (PS) on the color stability and shelf-life of cooked pork patties during refrigerated storage for 28 d were investigated. Nitrite was added at a concentration of approximately 120 ppm. Five treatments were formulated as follows: Control (120 ppm nitrite), T1 (2% PS), T2 (2% pre-converted nitrite from celery powder; PC), T3 (1% PS + 60 ppm nitrite), and NC (nitrite-free). The T1 and T3 samples had higher nitrosoheme pigment contents, which were associated with the redness of the samples (p<0.05). T1 resulted in the highest redness value (p<0.05). The redness and yellowness of the cooked pork patties increased with increasing PS levels. The pH of the samples subjected to all treatments decreased with progress of the storage period (p<0.05). The pH of the T1 and T3 samples treated with PS was lower (p<0.05) than that obtained with other treatments, as PS has a lower pH value. The treatments in which PS was added were most effective for reducing the level of thiobarbituric acid reactive substances (TBARS) and the residual nitrite content relative to the control. T1 resulted in the highest flavor, off-flavor, and overall acceptability scores during storage (p<0.05). The total viable bacterial count for all treatments was below 1 Log CFU/g, and E. coli and coliform bacteria were not detected during storage. Therefore, these results suggested that pre-converted nitrite from Swiss chard powder is a potential replacement for nitrite in meat products.

Changes in Quality Characteristics of Pork Patties Containing Multilayered Fish Oil Emulsion during Refrigerated Storage

  • Jo, Yeon-Ji;Kwon, Yun-Joong;Min, Sang-Gi;Choi, Mi-Jung
    • 한국축산식품학회지
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    • 제35권1호
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    • pp.71-79
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    • 2015
  • This study was performed to determine the effect of multilayered fish oil (FO) emulsion without or with trans-cinnamal-dehyde on pork patties. Multilayered FO (-primary, -secondary, -tertiary) emulsions were prepared using a layer-by-layer deposition technique with Tween 20, chitosan, and low methoxyl pectin, and were added to pork patties at the same concentration. Pork patties were then stored for 20 d in a refrigerator ($5^{\circ}C$) to study changes in quality. The results showed that the pH value of all samples significantly decreased but cooking loss increased during storage (p<0.05). However, water-holding capacity and moisture content showed no remarkable difference between treatments and storage periods (p>0.05). All pork patties containing multilayered FO (treated samples) showed higher values for lightness and significantly lower values for yellowness compared to control pork patties (untreated sample). Lipid oxidation was higher in treated pork patties than in control pork patties during storage. In addition, lipid oxidation and total viable bacterial count in pork patties decreased as the number of coating layers increased. However, hardness, cohesiveness, and springiness of all samples showed no significant change during storage (p>0.05) as compared to fresh pork patties. Furthermore, these did not remarkable change with addition of trans-cinnamaldehyde in all pork patties. From our results, we suggest that FO emulsion did not affect the texture characteristics of fresh pork patties, indicating that it could be used to improve the quality of pork patties by contributing high-quality fat such as unsaturated fatty acids.

돼지후지육 첨가 넙치(Paralichthys olivaceus) 스테이크의 제조 및 품질 특성 (Processing and Quality Properties of Olive Flounder Paralichthys olivaceus Steak Added with Pork Leg)

  • 윤문주;이재동;강경훈;박시영;주종찬;김정균
    • 한국수산과학회지
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    • 제48권6호
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    • pp.849-856
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    • 2015
  • This study was performed to obtain basic data regarding the development of fish steak products using olive flounder and pork leg. Olive flounder and pork leg were ground separately in a chopper. The methods used for processing were as follows. Chopped olive flounder (100 g) and other ingredients (bread crumbs, 13 g; onion, 12 g; garlic, 4 g; egg wash, 18 g; salt, 0.05 g; pepper, 0.05 g) were mixed in a chopper. The mixture was molded into a steak shape ($12{\times}7cm$) and roasted in an oven at $180^{\circ}C$ for 12 min (OF). FP consisting of a mixture of olive flounder (70 g) and pork leg meat (30 g) and OP consisting of pork leg meat alone (100 g) were processed according to the same procedure as described for OF. Various factors (viable bacterial count, chemical composition, pH, salinity, hardness value, color value, total amino acid content, free amino acid content, fatty acid composition, mineral content) were measured, and sensory evaluation was conducted. Based on the results of the sensory evaluation and hardness value, OP was deemed to be the most desirable, followed in order by FP and OF. There was a slight but significant difference between OP and FP.

해수산 무지개송어(Oncorhynchus mykiss) 프레임 육포의 위생적 및 영양적 특성 (Nutritional and Microbiological Characterization of Fish Jerky Produced Using Frame Muscle of the Sea Rainbow Trout Oncorhynchus mykiss)

  • 김민우;김용중;김민주;이수광;박선영;최병대;허민수;김진수
    • 한국수산과학회지
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    • 제49권3호
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    • pp.263-269
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    • 2016
  • Large quantities of by-products—such as fish frame, head, skin and viscera—are generated during seafood processing, and these by-products are not utilized extensively. Therefore, sea rainbow trout Oncorhynchus mykiss (SRT) frame muscle (FM) jerky was prepared by grinding SRT-FM, followed by mixing with seasoning, forming and drying. The nutritional and microbiological characteristics of the SRT-FM jerky were then investigated. The proximate composition of the SRT-FM jerky was 19.1% moisture, 38.7% crude protein, 7.9% crude lipid and 4.4% ash. The viable bacterium count of the SRT-FM jerky was 3.9 log CFU/g, and Escherichia coli and Staphylococcus aureus were not detected. The total amino acid content of SRT-FM jerky was 37.3 g/100 g, and the major amino acids were aspartic acid, glutamic acid, leucine and lysine. Based on the recommended daily intake of fish jerky (100 g), the most abundant mineral was potassium. The fatty acid composition of the SRT-FM jerky was 26.2% saturated acid, 34.5% monoenoic acid and 39.3% polyenoic acid, and the major fatty acids were 16:0, 18:1n-9, 18:2n-6, 20:5n-3 and 22:6n-3. These results suggest that SRT-FM jerky has high nutritional value.

굴 패각 가루와 함초 가루를 첨가하여 제조한 갓김치의 품질특성과 저장성 (Quality Characteristics and Storage Properties of Gat Kimchi added with Oyster Shell Powder and Salicornia herbacea Powder)

  • 정복미;정선진;김은실
    • 한국식품조리과학회지
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    • 제26권2호
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    • pp.188-197
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    • 2010
  • In this study, the quality characteristics and storage properties of gat kimchi added with oyster shell powder and Salicornia herbacea powder were investigated during a storage time of 80 days at $5^{\circ}C$. After storage for 80 days, the average calcium contents were significantly higher in the kimchi containing the oyster shell powder and Salicornia herbacea powder than the control. In addition, the average hardness value was significantly higher in the OS4 group(oyster shell powder at 4% and Salicornia herbacea powder at 2%) than the control group, as well as the OS10 group(oyster shell powder at 10% and Salicornia herbacea powder at 2%) during storage for 80 days. The hunter b value of the OS4 group was significantly lower than the control group. During fermentation, gat kimchi containing the oyster shell powder and Salicornia herbacea powder had a higher pH and a lower acidity value than the control group. In terms of sensory evaluation, there were no significant differences between the control and calcium-added kimchi during fermentation. After 40 days of storage, the OS4 group showed a lower total viable count, as well as lower lactic acid bacteria, yeast and E. coli, as compared with the control and OS10 groups. Based on the microbial load, the gat kimchi containing the oyster shell powder showed limitations in terms of shelf life.

Candida albicans의 Amphotericin B 및 Ketoconazole에 대한 감수성과 성장기와의 상호관계 (Growth Phase in Relation to Amphotericin B and Ketoconazole Susceptibilities of Candida albicans)

  • 고춘명;김수기
    • 대한미생물학회지
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    • 제22권4호
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    • pp.435-443
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    • 1987
  • A total of 30 strains of Candida albicans were examined for susceptibility to amphotericin B and ketoconazole using Sabouraud's dextrose broth, Kimmig broth and Supplemented yeast nitrogen base broth media. Furthermore, the growth curve and colony forming units were checked for use of stationary-phase cells and 2-hour incubation cells in the absence of atifungal agents. The viable counts were determined periodically during incubation by standard plate count techniques. The minimum inhibitory concentrations of amphotericin B for use of stationary phase cells were as follows: SDB, $0.09{\sim}0.97mcg/ml$(0.39mcg/ml); Kimmig broth, $0.19{\sim}0.39mcg/ml$(0.42 mcg/ml) and SYNB, $0.19{\sim}0.39mcg/ml$mcg/ml(0.23mcg/ml). In ketoconazole, MICs were value SDB, $3.12{\sim}25.0mcg/ml$(12.5mcg/ml); Kimmig broth, $12.5{\sim}25.0mcg/ml$ (22.5mcg/ml) and SYNB, $3.12{\sim}12.5mcg/ml$(6.71mcg/ml). The MICs of amphotericin B(0.2mcg/ml cone.) for use of 2-hour incubation cells in absence of AMB were, SDB, $0.04{\sim}0.39mcg/ml$(0.11mcg/ml); Kimmig broth, $0.09{\sim}0.39mcg/ml$(0.18mcg/ml) and SYNB, $0.09{\sim}0.19mcg/ml$(0.14mcg/ml) and in KTZ, the value of MICs were SDB, $3.12{\sim}25.0mcg/ml$(12.22mcg/ml); Kimmig broth, $0.78{\sim}25.0mcg/ml$(11.01mcg/ml) and SYNB, $1.56{\sim}12.5mcg/ml$(3.90mcg/ml). The two-log reductions in CFU per milliliter observed when 2 hour preincubation cells were treated with 0.2mcg/concentrations of AMB and 25.0mcg/ml of KTZ. However, AMB treated cells were restored to growth activity, it suggested that the AMB has no active antifungal activity.

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김치용 배추 절임 염수의 재사용 가능성 평가 (Evaluation of Brine Recycling on Salting of Chinese Cabbage for Kimchi Preparation)

  • 신동화;홍재식;오진아;안용선
    • 한국식품위생안전성학회지
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    • 제15권1호
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    • pp.25-29
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    • 2000
  • 김치 공장에서 배추 절임시 사용하는 염수의 재사용 가능성을 확인하기 위하여 염지 후 배출되는 염수의 염농도를 재조정하여 6회까지 재 사용하면서 염수의 성분과 미생물 변화 및 매회 절임 배추로 담근 김치의 품질을 평가하였다. 배추 절임 후 염수의 염 농도는 1.35~2.49%감소하였고 재 사용횟수에 따라 절임 후 염수의 pH는 4회까지 유의적 변화를 보였고 절임 전 염수의 pH는 4회 까지는 통계적 유의차가 없었으며 산도도 절임 횟수에 따라 증가하는 경향을 보였다. 염수 중 미생물수는 1회 사용시 1.58$\times$10에서 4회 사용시 2.25$\times$$10^{7}$ 으로 최고에 달하여 10배정도 상승하였다. 염수 중 염을 제외한 순 가용성 고형분은 6회 사용시 0.93%로 미미하게 증가하는 경향이었고 비타민 C함량은 6회사용 후 0.55 mg%로 재사용 횟수에 따라 상승하는 경향을 보였다. 6회까지 염수를 재 사용하여 절임한 배추로 담근 김치의 pH와 산도, 그리고 관능적 품질은 유의적 차이를 보이지 않아 염수의 재 사용가능성을 확인하였다.

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대두요구르트 제조에 의한 대두의 품종별 비교 (Comparison of Cultivars of Soybean by Soy Yoghurt Production)

  • 차성관;최병권;김길환
    • 한국식품과학회지
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    • 제22권3호
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    • pp.357-362
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    • 1990
  • 19 품종의 대두로부터 제조된 두유에 L. acidophilus KCTC 2182를 접종하여 24시간 발효시킨 후 품종별 특성을 비교 검토하였다. 대두요구르트의 조단백질 함량은 $2.58{\sim}2.91%$로 평균 2.74%이었으며 조지방은 $1.34{\sim}1.85%$로 평균 1.49%이었다. 대두로부터 대두요구르트로 이행된 각 성분의 회수율은 고형분이 $52.14{\sim}61.54%$로 평균 56.17%이었으며 단백질의 수율은 $59.22{\sim}67.50%$로 평균 63.32%, 지방의 수율은 $65.80{\sim}86.02%$로, 평균 72.57% 수준이었다. 대두요구르트의 pH값은 $4.53{\sim}5.03$이었고, 적정산도는 $0.20{\sim}0.41%$이었으며, 생균수는 $3.83{\times}10^8{\sim}3.22{\times}10^9/ml$이었다. 분리된 유청의 양은 두유 100ml당 $1.4{\sim}8.21ml$이었다.

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고추가루의 저장성(貯藏性)과 방사선 처리효과(處理效果) (Storage Stability and Irradiation Effect of Red Pepper Powder)

  • 이정혜;최언호;김형수;이서래
    • 한국식품과학회지
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    • 제9권3호
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    • pp.199-204
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    • 1977
  • 경기도 이천(利川)에서 수확한 고추(새마을 금장고추 2호)를 건조제분하여 평형수분(平衡水分)을 측정하였으며 125, 250, 500, 1,000 krad의 Co-60 감마선을 조사(照射) 후 저장 중 capsaicin, capsanthin 및 미생물 수의 변화를 조사하여 다음과 같은 결과를 얻었다. 1) $25^{\circ}C$에서 고추의 평형수분함량은 상대습도 43.7, 53.3, 64.4, 75.4, 84.3%에서 각각 13, 18, 25, 28, 37%이었다. 2) 제분도(製粉度)가 낮을수록, 즉 고추가루의 입경(粒徑)이 클수록 평형수분함량이 낮아 장기저장에 유리하였다. 3) 고추가루중의 생존(生存)미생물 수는 g당 약 $10^8$개나 되었으며 혼합미생물의 $D_{10}$ 값은 210 krad이었다. 4) 수분, capsanthin은 500, 1,000 krad 조사선량(照射線量)에서 저장중 변화가 거의 없었으나 capsaicin은 방사선조사(照射)에 의하여 부분적으로 파괴되었다.

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