• 한국환경보건학회지
• /
• 제2권1호
• /
• pp.21-24
• /
• 1975

During April and May, 1973 rain water samples were collected five different times in nine selected areas of Seoul city. The pH factor and bacteria counts were measured. The following results were obtained. 1. The lowest pH was 6.18 at Moon Rae Dong and the highest was 6.30 at Jong Am Dong. This difference is not significant at the 0. 05 level. 2. The pH factor did not vary significantly with the time of sample collection. 3. Dae Bang Dong had the highest Total Plate Count of $1.9\times 10^3/ml$, Bo Moon Dong and An Kook Dong had the lowest Total Plate Count at $4.1\times 10^2/ml$. 4. The total plate count varied greatly according to the time of sample collection. This result can be used to remove are pollutants. 5. The Total plate Count is nearly inversely proportional to the length of time during which the sample is collected.

• 한국축산식품학회지
• /
• 제18권1호
• /
• pp.27-34
• /
• 1998

This study was carried out to evaluate of the microbial and sensory quality of ready-made hamburger and sandwich. Initial total plate count of hamburger for establishment A and B were 1.2$\times$102 cfu / g and 3.4$\times$102 cfu / g, respectively, and for establishment C was 7.9$\times$104 cfu / g. After 48 hour storage at 1$0^{\circ}C$, total plate count of hamburger for establishment A and B increased to 1.2$\times$104 cfu / g and 6.8$\times$103, respectively, and for establishment C increased to 1.2$\times$107 cfu / g. Initial total plate count of sandwich for establishment A and B were 3.2$\times$102 cfu / g 7.9$\times$102 cfu / g, respectively, and for establishment C was 1.1$\times$105 cfu / g. After 48 hour storage at 1$0^{\circ}C$, total plate count of hamburger for establishment A and B increased to 8.1$\times$103 cfu / g and 2.3$\times$104, respectively, and for establishment C increased to 4.4$\times$108 cfu / g. No E. coli, Salmonella, Vibrio, and Staphylococcus aureus were detected under simulated storage conditions. There was no significant changes in pH, acid value, and volatile nitrogen number under simulated conditions. In sensory evaluation of hamburger and sandwich, sensory score was lowered by increase of total plate count.

• 한국환경보건학회지
• /
• 제33권5호
• /
• pp.422-427
• /
• 2007

The purposes of this study were to investigate the concentration of total coliforms in sewage effluents during the period from August 2004 to October 2005. The removal efficiency range of multi-tube method and plate count method were $31.3{\sim}99.5%$ and $66.8{\sim}99.2%$, respectively. Though a correlation between the multi-tube method and the plate count method in the same sample is low, not only is an experimental procedure very simple, but the time required also is short. The seasonal correlation between methods showed more sensitive spring and summer than autumn and winter. So the study indicated plate count method can be used in rapid and reliance identification of total coliform more than the multi-tube method.

• 한국식품위생안전성학회지
• /
• 제17권2호
• /
• pp.55-60
• /
• 2002

In order to investigate the microbiological contamination of spring water, we performed the standard plate count, coliform and psychrotrophilic bacteria in 109 spring waters in Seoul. Of 109 spring waters, geometirc mean standard plate count was 0.19 CFU/ml, and the highest in Mt. Boolam, 4..43 CFU/ml and Mt. Dobong, 3.86 CFU/ml, but not detected in Mt. Woomyun and Mt. Cheonggye. Four spring waters have shown over 100 CFU/ml in standard plate count. The geometric mean psychrotrophilic bacteria was 49.2 CFU/ml, the most prevalent spring water was Mt. Nam, 125 CFU/ml, the lowest Mt. Woomyeun. Among a total of 109, coliform was detected from 21 spring waters(19.3%) and the geometric mean of coliform was 0.005 MPN/100ml. The isolated genera of coliform were 7 isolates of E. coli(33%), 5 Klebsiella(24%), 4 Enterobacter(19%), 3 Citrobacter(14%) and 2 Serratia(10%), respectively. The 22 spring waters(20.2%) failed to meet the standard limits of drinking water based on regulation in Korea. The inappropriate rate of standard plate count in spring water was 4.5%, that of coliform was 81.1% and both of them was 13.6%. There was the significant correlation between standard plate count and psychrotrophilic bacteria in spring water(r=0.95, p<0.01).

• 한국환경보건학회지
• /
• 제42권2호
• /
• pp.126-132
• /
• 2016

Objectives: This study aims to provide basic data regarding the bacterial total plate count in the atmospheric environment for related studies. Methods: Total plate count and the identification of culturable bacteria in the atmospheric environment in Incheon took place in 2015 using periodic survey. Correlationship analysis was performed between the number of culturable bacteria and environmental elements. In addition, an estimation of novel bacterial species was undertaken using the similarities and phylogenetic tree based on the 16S rRNA gene. Results: The total plate count of culturable bacteria was on average $176CFU/m^3$, and did not exceed $610CFU/m^3$ in the atmospheric environment. Periodic monthly measuring of total plate count was highest in June at $293CFU/m^3$, while the lowest was in July at $125CFU/m^3$. Furthermore, as a result of the identification of culturable bacteria, the genera Arthrobacter and Kocuria were dominant, while novel bacterial taxa that belong to the genera Chryseobacterium and Herbiconiux were separated. Conclusion: The total number of culturable bacteria from the atmospheric environment in Korea is on average $176CFU/m^3$. In addition, the genera Arthrobacter and Kocuria dominate. The presence of novel bacterial taxa are expected in the atmospheric environment, such as belonging to the genera Chryseobacterium and Herbiconiux.

• 한국환경보건학회지
• /
• 제36권2호
• /
• pp.141-147
• /
• 2010

Indicator bacteria of fecal pollution were enumerated and compared by various detection methods for influent and final effluent of a sewage treatment plant. Total coliforms were enumerated by four methods including most probable numbers, chromogenic enzyme substrate test, membrane filtration, and plate counts and were about $10^4$ for influent and $10^2{\sim}10^3\;CFU/ml$ for final effluent. Fecal coliforms ranged between $10^3$ and $10^4$ for influent and $10^2\;CFU/ml$ for effluent by chromogenic enzyme substrate test and membrane filtration. Fecal streptococci counts were 1-log less than fecal coliforms counts, $10^2{\sim}10^3$ for influent and $10^1\;CFU/ml$ for effluent. Total coliforms numbers by plate count both in influent and in effluent showed 1-log higher than by the other three methods. Statistical analysis revealed that numbers of total coliforms by plate count in final effluent had the highest average of correlation (r=0.778, p<0.01) compared with those by the other three methods. In addition, total coliforms numbers by plate count showed most significant correlation (r=0.835, p<0.01) with those by chromogenic test which is well-known as its highest recovery efficiency. These results suggest that the plate count would be the optimum detection method for total coliforms in wastewater treatment plants which are the only microbiological standard of final effluent from wastewater treatment plants in the Republic of Korea, considering economic aspects and difficulties in laboratories.

• 한국식품위생안전성학회지
• /
• 제19권4호
• /
• pp.209-216
• /
• 2004

우리나라 전통식품인 김치, 된장, 고추장, 간장. 탁주, 식혜, 수정과에 있는 미생물 분석을 위하여 건조필름법과 전통적인 미생물 분석법을 비교하였다. 일반세균 분석에는 plate count agar 법과 $Petrifilm^{TM}$ aerobic count plate 법을 비교하였고, 대장균군과 대장균의 분석에는 most probable number (MPN) 법과 $Petrifilm^{TM}$ coliform count plate 및 $Petrifilm^{TM}$ E. coli/colmfom count plate 법을 각각 비교하였으며, 효모와 곰팡이의 분석에는 potato dextrose agar 법과 $Petrifilm^{TM}$ yeast and mold count plate 법을 비교하였다. 황색포도상구균의 분석에는 coagulase 시험법과 $Petrifilm^{TM}$ Staph express fount plate 법을 비교하였다. 두 방법간의 상관계수는 일반세균이 0.974-0.998, 대장균군이 0.955-0.978, 대장균이 0.968-0.986, 효모와 곰팡이가 0.913-0.995, 황색포도상구균이 0.998-0.999로 두 방법간의 상관성이 매우 높았으며, 평균 미생물수에 있어서도 유의적인 차이가 없었다(p>0.05). 이러한 결과로부터 건조필름법이 기존의 전통적인 방법을 대체할 수 있는 미생물 분석법임이 확인되었다.

• 한국식품과학회지
• /
• 제26권6호
• /
• pp.764-769
• /
• 1994

본 실험은 원유내외 세균을 빠르고, 일관성있고, 신뢰성이 있는 평가 system을 얻기 위함이며, 원유 내외 총균수와 저온성균수, 대장균군수를 malthus의 detection time과 regression equation과 상관관계를 조사하였다. Conductance method는 종래의 plate count method보다빠르고 자동적이며, 노동력을 최대한 절감할 수 있다. 그 결과는 다음과 같다. 1. Conductance detection time을 (Y), total bacterial log count를 (X)라고 할 때 regression equation Y=18.27651-2.07550X, 상관계수는 -0.95(n=201)로 나타났다. 2. Conductance detection time을 (Y), total bacterial log counts를 (X)라고 할때 regression equation Y=9.32048-1.15598X, 상관계수는 -0.90(n=207)로 나타났다. 3. Conductance dotection time을 (Y), psychrotrophic bacterial log counts를 (X)라 할때 regression equation Y=29.96008-3,02487X 상관계수는 -0.90(n=201)로나타났다.

• 동아시아식생활학회지
• /
• 제15권4호
• /
• pp.419-430
• /
• 2005

In this study, to prove the effect of soybeans's electron donating ability and its addition on eel gouem, storage and sensory characteristics were examined. During the storage period, pH values of all gouem samples were decreased with initial increase except the control. BSB $3\%$ sample had the lowest pH value and YSB $10\%$ sample was the highest 6.71 in pH value at first storage day. The electron donating ability of soybeans were increased with the amount of addition. The electron donating ability of SBSB 20g sample was the highest, which was 2 ${\~}$ 3 times higher than that of YSB sample. L values of all the gouem samples had a tendency to decrease according to the storage period and amount of addition. All samples' a values were increased throughout the storage period, while b values decreased except the control and GNG sample. TBA and VBN values of all the samples were up and down throughout the storage period SBSB $10\%$ sample had the lowest in TBA value. The electron donating ability of all soybean samples and TBA value of eel gouem samples showed a negative correlation. Total plate count of bacteria was increased rapidly for initial storage period, but all samples showed a tendency of slow growth after the 8th day of storage. During all storage days, SBSB, BSB samples had a similar tendency of low total plate count growth. GNG $10\%$ sample showed the lowest in total plate count of bacteria at sixteenth day of storage. Sensory scores of all samples decreased with storage. For all the items of the sensory evaluation, SBSB $5\%$ sample got the highest score among all eel gouem samples. GNGSB sample showed the lowest in TBA value and total plate count of bacteria. And in the sensory evaluation, GNGSB sample got the highest scores for several items.

• 한국식품조리과학회지
• /
• 제19권5호
• /
• pp.591-595
• /
• 2003

The objective of this study was to evaluate the effects of ingredients on the change of Samgyetang quality during refrigerated storage. The Samgyetang was prepared with five treatments. The five treatments were chicken cooked alone (Tl), cooked with jujubi, ginseng and garlic (T2), cooked with jujubi (T3), cooked with ginseng (T4) and cooked with garlic (T5). The TBA values of the Samgyetang over 4 days of refrigerated storage were T1(0.89) > T3(0.74) T5(0.74) > T4(0.57) > T2(0.42). The total plate counts of the Samgyetang in the T2 and T3 treatments were lower than with the other treatments. The coliform counts of the Samgyetang in the T2 and T5 treatments were lower than with the other treatments. The results from this study showed that ginseng had an antioxidant activity, jujubi lowered the total plate count and garlic lowered the coliform count in refrigerated Samgyetang. Therefore, the addition of these ingredients maintains the quality of Samgyetang during refrigerated storage.