To determine the true digestible phosphorus (TDP) requirement of growing pigs, two experiments were designed with the experimental diets containing five true digestible P levels (0.16%, 0.20%, 0.23%, 0.26% and 0.39%) and the ratio of total calcium to true digestible P (TDP) kept at 2:1. In Experiment 1, five barrows (Duroc${\times}$Landrace${\times}$Yorkshire) with an average initial body weight of 27.9 kg were used in a $5{\times}5$ Latin-square design to evaluate the effect of different dietary P levels on the digestibility and output of P and nitrogen. In Experiment 2, sixty healthy growing pigs (Duroc${\times}$Landrace${\times}$Yorkshire) with an average body weight (BW) of 21.4 kg were assigned randomly to one of the five dietary treatments (12 pigs/diet), and were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. The results indicated that the true digestibility of P increased (p<0.05) linearly with increasing dietary TDP level below 0.26%. The true P digestibility was highest (56.6%) when dietary TDP was 0.34%. Expressed as g/kg dry matter intake (DMI), fecal P output increased (p<0.05) linearly with increasing P input. On the basis of g/kg fecal dry matter (DM), fecal P output was lowest for Diet 4 and highest (p<0.05) for Diet 5. The apparent digestibility of crude protein (CP) did not differ (p>0.05) among the five diets, with the average nitrogen output of 12.14 g/d and nitrogen retention of 66% to 74% (p>0.05), which suggested that there was no interaction between dietary P and CP protein levels. During the 28-d experimental period of Experiment 2, the average daily gain (ADG) of pigs was affected by dietary TDP levels as described by Eq. (1): $y=-809,532x^4+788,079x^3-276,250x^2+42,114x-1,759$; ($R^2=0.99$; p<0.01; y = ADG, g/d; x = dietary TDP, %), F/G for pigs by Eq. (2): $y=3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2=0.99$; p<0.01; y = F/G; x = dietary TDP, %), and Total P concentrations in serum by Eq. (3): $y=-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ (R2 = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest F/G (1.07) and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet at a total Ca to TDP ratio of 2:1.
A growth trial (Expt. 1) and an in vitro fermentation experiment (Expt. 2) were conducted to determine the response of growth performance, cecal fermentation characteristics and in vitro gas production to incremental levels of substitution of digestible fiber for lignified fiber in the diet of weaned rabbits. Three diets, formulated by substituting soyhulls (SH; used as digestible fiber source) for soybean straw (used as lignified fiber source) at substitution levels of 0, 25 and 50%, were used in a factorial design. In the growth trial (Expt. 1), increasing levels of SH substitution resulted in a quadratic increase in daily body weight gain rate (p<0.04) and feed conversion efficiency (p<0.02), but in a numerical decrease in dietary DM intake (p=0.15). When SH were included in the diet at 25% substitution level, rabbits had the highest rate of liveweight gain and feed conversion efficiency. As SH substitution level increased, pH values and ammonia-N of cecal contents linearly (p<0.001) decreased, but total VFA concentration linearly (p<0.03) increased. With incremental levels of SH substitution, the percentage of acetate and butyrate linearly (p<0.05) reduced, but the percentage of propionate and minor acids linearly (p<0.03) increased. Increasing the SH substitution levels tended to increase incidence of diarrhea. In the in vitro fermentation experiment (Expt. 2), regardless of origin of substrates fermented, increasing SH substitution level resulted in increased maximal gas production (p<0.001) and shortened gas production lag time, but had no effect on gas production rate (p>0.2). These observations suggest that incrementally feeding SH to rabbits could stimulate their cecal microbial activity, allowing cecal fermentation to shift towards favoring fiber digestion. In conclusion, digestible fiber from soyhulls may partially substitute for more lignified fiber, soybean straw, without having an adverse effect on cecal fermentative and microbial activity and growth performance. For growing rabbits, about 73% of total dietary NDF should be supplied by effective NDF, the remainder could come from digestible NDF, such as soyhulls.
Song, Yowook;Kim, Dong Woo;Kim, Jeongtae;Fiaz, Muhamad;Kwon, Chan Ho
한국초지조사료학회지
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제37권2호
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pp.101-107
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2017
Corn is basal forage for livestock species in Republic of Korea but it lacks protein and needs nitrogenous fertilizer. This study was designed with main objective to achieve optimum growth, yield & nutritive value of forage for livestock through implementing corn-soybean intercropping strategy at 17 different places under Korean condition. Two treatments; corn as monocrop (control treatment) and corn-soybean intercrop were compared under Randomized Block Design from $28^{th}$ May to $8^{th}$ October, 2015. Each treatment had three replicates in each block, whereas seventeen different places were considered as blocks. Data were analyzed through SAS-9.1.3 software. Difference between two treatment means was tested through T-test. Findings depicted that intercropping pattern could not influence (P>0.05) corn plant & ear height, corn lodged stalk No. and corn stalks number. However, corn-soybean intercropping enhanced (P<0.05) forage productivity in terms of total fresh yield ($16.4{\pm}0.7^b$ vs. $19.9{\pm}0.7^atons\;ha^{-1}$), total dry matter yield ($5.38{\pm}0.25^b$ vs. $6.41{\pm}0.31^atons\;ha^{-1}$) and total digestible nutrients yield ($3.94{\pm}0.17^b$ vs. $4.59{\pm}0.21^atons\;ha^{-1}$). Dry matter percentage in corn stalks and corn ears was not different (P>0.05) between two treatments. It was concluded that corn-soybean intercropping strategy was promising technique in enhancing forage productivity though positive symbiotic relation between two crops.
This study was carried out to investigate the effects of seeding dates on the yield and feed value of whole-crop barley cultivated in paddy soil. The field experiment was conceived as a randomized block design performed in triplicate with seeding dates of Oct. 19 (T1 as treatment 1), Oct. 26 (T2 as treatment 2), Nov. 2 (T3 as treatment 3), Nov. 9 (T4 as treatment 4), and Nov. 16 (T5 as treatment 5) as treatments. The barley grown in all treatments was harvested on May 21 of the following year. Plant length, fresh yield, dry matter yield, and total digestible nutrient (TDN) yield were higher (p < 0.05) in barley with early seeding dates, whereas crude protein and ether extract were higher (p < 0.05) with late seeding dates. Crude fiber and neutral detergent fiber were highest in the T3 treatment and lowest in the T1 treatment. There was no significant difference between acid detergent fiber and TDN among the different seeding dates. Total mineral contents were higher in the order T1 > T2 > T3 > T4 > T5 (p < 0.05), whereas the total amino acid content increased significantly (p < 0.05) with a delay in seeding date. Free sugar contents (fructose, glucose, and sucrose) were higher with early seeding dates (p < 0.05). Collectively, the results obtained in this study indicate that it is favorable to seed soon after harvesting rice to increase dry matter and TDN yields and mineral and free sugar contents of whole-crop barley in the midlands of Korea.
Meat and bone meal is a valuable protein and mineral source in diets of production animals and contributes to the protein, energy and mineral component of diets. The aim of the present study was to more accurately characterise the apparent ileal amino acid digestibility of meat and bone meals produced in New Zealand and evaluate routine in vitro assays used in practise to measure meat and bone meal quality. A total of 94 commercial meat and bone meals from 25 New Zealand rendering plants over a two and a half year period were analysed for proximates, gross energy, gross amino acid content (incl. hydroxyproline, hydroxylysine and lanthionine), apparent ileal amino acid digestibility, pepsin nitrogen digestibility, protein solubility and bone content. The mean crude protein content of the 94 meat and bone meal samples was 56.8% with a range of >35% units and a coefficient of variation of 9.8%. The mean crude fat and ash content were 10.0 and 28.4% respectively. These latter components showed a large range (16 and 43%, respectively) with coefficients of variation above 22%. Amino acid digestibility between samples was highly variable with lysine and sulphur amino acids digestibility ranging between 45.8-89.0 and 38.2-85.5%, respectively. Pearson correlation coefficients are presented between crude protein content and individual gross amino acids, crude protein content and individual digestible amino acid content, and pepsin N digestibility and individual digestible amino acid content. There was a significant relationship between the digestible amino acid nitrogen content and the crude protein content while pepsin nitrogen digestibility was not correlated to ileal amino acid nitrogen digestibility (r=-0.06). Meat meals with a high protein content had relatively low hydroxyproline and hydroxylysine levels something that was attributed to the levels of collagen from bone. The data indicated that lanthionine (formed upon heat treatment of cysteine with a hydroprotein) is not a good indicator of the heat treatment employed to meat and bone meals. Step-wise multiple regression equations to predict the apparent digestible content of amino acids from rapid in vitro assays are presented. The most selected variables included ash and crude fat content. In general the equations derived for the essential amino acids had a higher degrees of fit (R2) compared to the non-essential amino acids. The R2 for the essential amino acids ranged from 0.43 for histidine and 0.68 for leucine. These equations provide a means of more rapidly estimating the apparent ileal digestible amino acid content (protein quality) of meat and bone meal using standard analyses.
De Oliveira, Gisele Cristina;Moreira, Ivan;De Souza, Ana Lucia Pozzobon;Murakami, Alice Eiko;Parra, Angela Rocio Poveda;De Oliveira Carvalho, Paulo Levi;Borile, Maicon Danner
Asian-Australasian Journal of Animal Sciences
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제24권7호
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pp.982-992
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2011
Three experiments were carried out to determine the nutritional value and to verify the pig performance in growing and finishing phases (30 to 90 kg) fed on diets containing common corn (CC), high-lysine corn (HLC) and high-oil corn (HOC). In the total digestibility trial (Exp. I) 12 barrows were used. Values of digestible energy (DE) and metabolizable energy (ME) as-fed basis for CC, HLC and HOC, were: 3,396 and 3,275 kcal/kg; 3,248 and 3,139 kcal/kg; 3,445 and 3,308 kcal/kg, respectively. In order to determine the apparent and true ileal digestibility coefficients of amino acids, as well as the values of true digestible amino acids of the CC, HLC and HOC, an ileal digestibility trial was done (Exp. II) with T-cannulated barrows ("T" simple). The treatments consisted of three diets, with one of them as the sole source of protein (CC, HLC and HOC). In the performance experiment (Exp. III), 36 crossbred pigs, allotted in a completely randomized design with three treatments and 12 replications were used. Treatments consisted of three diets: 1 - CC; 2 - HLC and 3 - HOC. It was observed no difference for performance and carcass variables among the corns with different nutritional profiles. Results of the three experiments highlighted the importance of segregating corns in their real chemical and energetic composition as well as the values of true digestible amino acids for formulating diets for growing and finishing pigs.
The gross composition, gross amino acid content, apparent ileal amino acid digestibility and apparent ileal digestible amino acid content from 64 commercially produced meat and bone meals were statistically analysed. The samples were produced by 22 plants over a 2.5 year period with eight plants using batch dry rendering and 14 plants using low temperature rendering. A linear model with method and time of year (period) as fixed effects, plant within method as a random effect and sheep percent as a covariate was fitted to the composition data. The majority of the variation in the gross composition, amino acid digestibility and digestible amino acid content was explained by differences between plants using the same method. Neither rendering season nor origin of the raw materials contributed significantly to the observed variation in meat and bone meal protein quality. Rendering method (low temperature or batch rendering) had a significant effect on the variation observed in gross fat content, gross energy content, pepsin nitrogen digestibility, protein solubility and total lanthionine content. The digestibility of a number of amino acids and the apparent digestible content of arginine, cysteine, aspartic acid, proline and hydroxyproline were also significantly affected by rendering method. On average, batch dry and low temperature rendering systems produce meat and bone meals of similar nutritional quality. The variation between plant and within plant, however, is large, indicating that purchasing meat and bone meal from the same plant does not guarantee a consistent quality.
Jiao, Ting;Lei, Zhaomin;Wu, Jianping;Li, Fei;Casper, David P.;Wang, Jianfu;Jiao, Jianxin
Animal Bioscience
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제34권11호
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pp.1776-1783
/
2021
Objective: This project aimed to evaluate the effects of both different additives and filling methods on nutritive quality, fermentation profile, and in situ digestibility of whole plant corn silage. Methods: Whole plant corn forage harvested at 26.72% dry matter (DM) was chopped and treated with two filling methods, i) fill silos at one time (F1), ii) fill silos at three times (F3), packing samples into one/three silo capacity at the first day, another one/three capacity at the second day, then one/three at the third day, three replicates. For each replicate, samples were treated with three additives, i) control (CTRL, no additive), ii) Sila-Max (MAX, Ralco Nutrition Inc., Marshall, MN, USA), and iii) Sila-Mix (MIX, Ralco Nutrition Inc., USA). With three replicates of each secondary treatment, there were nine silos, 54 silos in total. Each silo had a packing density of 137.61 kg of DM/m3. All silos were weighed and stored in lab at ambient temperature. Results: After 60 d of ensiling, all items showed good silage fermentation under MAX filled one time or three times (p<0.01). Higher silage quality for all additives was obtained at filling one time than that filled three times (p<0.01). The highest DM and lowest DM loss rate (DMLR) occurred to MAX treatment at two filling methods (p<0.01); Digestibility of acid detergent fiber, neutral detergent fiber (NDF), and curde protein had the same results as silage quality (p<0.01). Yield of digestible DM and digestible NDF also showed higher value under MAX especially for filling one time (p<0.05). Conclusion: All corn silages showed good fermentation attributes (pH<4.0). The forage filled one time had higher silage quality than that filled three times (p<0.01). MAX with homofermentative lactic acid bacteria enhanced the lactic acid fermentation, silage quality and nutrient digestibility, and so improved the digestible nutrient yield.
Ku, Yun;Ingale, Santosh Laxman;Kim, Jin Soo;Lee, Su Hyup;Kim, Young Hwa;Chae, Byung Jo
Journal of Animal Science and Technology
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제55권4호
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pp.263-272
/
2013
This experiment was conducted to investigate the effects of soybean meal (SBM) of different origin (Korea, India or Brazil) on performance, fecal microflora and economics of grower pigs. A total of 144 grower pigs (initial BW, 10.4 kg) were randomly allotted to six treatments on the basis of BW. Pig's diets containing SBM were formulated based on the total amino acid (TAA) or true ileal digestible amino acid (TIDAA) levels. Pigs fed domestic SBM diets had greater (p<0.05) overall ADG, ADFI and final body weight and apparent total tract digestibility (ATTD) of DM, GE and CP (phases I, II and III) than those fed diets containing SBM originated from India or Brazil. Moreover, greater (p<0.05) ADG, ADFI, feed /gain (F/G), and ATTD of DM, GE and CP were observed in response to diets formulated on a TIDDA basis when compared with those formulated on a TAA basis. The fecal microflora was not affected (p>0.05) by dietary treatments. The overall feed cost per kg body weight gain was less (p<0.05) for diets formulated using domestic SBM than for those containing SBM from India, while the feed cost per kg body weight gain was less (p<0.05) for diets formulated prepared on a TIDAA than a TAA basis. These results indicate that domestic SBM has better quality than SBM imported from Brazil or India and better performance was obtained when diets were formulated based on the true ileal digestible amino acid (TIDAA) content.
A CRD experiment with thirty growing cross bred calves were assigned at random to three treatments rations. 1) $T_0$, 0% Urea + 20% M. O. cake, 2) $T_1$, 1% Urea + 10% M. O. cake and 3) $T_2$, 2% Urea + 0% M. O. cake to develop a rice straw based ration for ruminants. Sweetish odour and yellowish colour were observed in good recovered silage. Organic matter varied from 87.45% to 89.63% whereas crude protein varied from 14.0% to 14.5% in each treatment. No significant differences were found among the nutrient composition of the ration. The dry matter in take (DMI) and dry matter digestibility was higher in $T_0$ (0% Urea) than those of ration containing 1% ($T_1$) and 2% Urea ($T_2$). The organic matter digestibility decreases with increasing doses of urea. The crude protein & nitrogen-free-extract digestibility were found higher in the ration $T_1$ containing 1% urea whereas crude fibre digestibility and available metabolizable energy (ME) were higher in $T_0$ containing no urea as compared to $T_1$ and $T_2$. Total digestible nutrient (TDN) decreases with the increase of urea level. The highest feed efficiency was found in $T_0$ having no urea and lowest was in $T_2$. The animals gained in weights from each ration. Highest gain in weight was found in $T_0$ ration, then followed $T_1$, and $T_2$. This is due to natural protein available in M. O. cake only. It is concluded that supplemetation of urea or M. O. cake with readily available energy source as molasses upto 20% of total dietary dry matter in a complete ration may increase the intake of low quality fibrous roughage only when nitrogen and mineral are not limiting factor.
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