• Journal of People, Plants, and Environment
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• v.23 no.4
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• pp.465-473
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• 2020

Background and objective: Aronia melanocarpa, called black chokeberry, is a natural product belonging to the family rosaceae, and is known to contain polyphenolic antioxidants including cyanidin-3-galactoside, cyanidin-3-arabinoside, cyanidin-3-xyloside, and cyanidin-3-glucoside Because of the abundance of anthocyanins, Aronia has been studied to be used in various industries. Methods: Aronia melanocarpa extract was treated 24 hours a day to RAW 264.7 cells with inflammations induced by LPS. After extracting total RNA, the amount of inflammatory cytokine expression was measured using RT-PCR. After processing the Aronia liposome using Aronia extract and the layer-by-layer electrostatic deposition method in keratinocyte cells at the same time, we checked the synthesis of Hyaluronic acid enhanced through the formation of Aronia liposome using ELISA. Results: The treatment of Aronia extract in inflammation-induced RAW 264.7 cells conducted to check the anti-inflammatory efficacy of Aronia extract inhibited inflammatory cytokines including TLR4, TNF-α, IL-1β, COX-2, and iNOS and increased the mRNA expression of HAS2 genes related to moisturizing. Based on the anti-inflammatory and moisturizing effect of Aronia extract, the Aronia liposome technology was introduced to Aronia extract to produce Aronia liposome. Conclusion: The liposome formation of Aronia extract is expected to be used as a functional material in treating various inflammatory skin diseases by controlling the moisture content of the corneocytes by increasing the expression rate of genes associated with the synthesis of hyaluronic acid, while retaining the efficacy of its components.

• The Journal of Engineering Geology
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• v.32 no.4
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• pp.571-583
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• 2022

On 7 August 2020, a large-scale catastrophic landslide was triggered by extreme rainfall at Osan village, Gokseong County, South Jeolla Province, South Korea. The initiation mechanism of the Gokseong landslide was different from those typical landslides that occurred in South Korea. Despite the relatively low elevation and slope degree, the landslide had a long runout distance of about 640 m over a total vertical distance of 90 m. A detailed field investigation and chemical analysis were conducted to understand the possible mechanisms for the high-speed and long-runout behavior of the landslide. The terrain controlled the motion behavior of the landslide and the seepage was observed at the whole landslide body. The clay-rich soils covered on granite bedrock of the landslide deposition area from the rice paddy field to the landslide crown. The results of this study may provide basic data for further research on the mechanisms for landslide initiation and propagation.

• Childhood Kidney Diseases
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• v.26 no.2
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• pp.80-85
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• 2022

Purpose: To determine the prevalence, clinical manifestations, and outcomes of renal involvements in pediatric Alagille syndrome (ALGS). Methods: A total of 21 patients diagnosed with ALGS at age under 18 years who visited Samsung Medical Center from March 1999 to March 2022 were enrolled. ALGS was diagnosed either by clinical manifestations, targeted JAG1 sequencing, and/or liver biopsy. Medical records including sex, age, renal manifestations, urinalysis, serum creatinine, JAG1 sequencing, and ultrasonography were retrospectively reviewed. Results: The male to female ratio was 9:12. The mean age of patients at confirmative diagnosis of ALGS was 18.4 months. Sanger sequencing was performed for 17 patients. Sixteen of 21 patients (76.1%) showed JAG1 mutations. Renal involvement was found in 10 patients (47.6%). The most common type of anomaly was renal dysplasia (40%). One patient having renal dysplasia was pathologically confirmed with glomerular lipid deposition. Two patients (20%) manifested nephrocalcinosis/nephrolithiasis. Among eight renal-involved patients who survived, four (50%) progressed to chronic kidney disease stage 3. Two of these chronic kidney disease patients were diagnosed with hepatorenal syndrome. The other four patients had renal functions preserved, including two without any interventions and two who underwent urological interventions. Conclusions: The current study revealed a high prevalence of renal involvement in Korean pediatric ALGS with diverse phenotypes.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.897-906
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• 2006

This study was conducted to determine effects of different supplementation periods (2, 3 and 4 months) of spent composts of Se-enriched mushrooms (Se-SMC) on plasma glutathione peroxidase (GSH-Px) activity and selenium deposition of finishing Hanwoo steers for the optimal supplementing period determination in order to produce Se-fortified Hanwoo beef. In the present study, 30 Hanwoo steers were allotted to treatments in six groups of five steers per pen. Treatments were separated into control and Se-SMC for each supplementation period. Dietary selenium contents were 0.1 and 0.9 ppm for control and Se-SMC treatments, respectively. At the end of each supplementation period, steers by periods were slaughtered to collect hind leg and liver samples for their selenium analyses. Blood samples were taken to analyze whole blood Se concentration and plasma GSH-Px activity at the last day of each supplementation period. Dry matter intakes were unaffected by Se-SMC and supplementation periods. In addition, average daily gain was not different between control and Se-SMC treatments and among supplementation periods. There was no difference for total body weight gain between control and Se-SMC treatments within each supplementation period. The supplementation of Se-SMC significantly (P<0.001) increased whole blood Se concentration, but whole blood selenium concentration was not affected by the supplementation period. Furthermore, plasma GSH-Px activity showed similar trend as shown in the pattern of whole blood Se concentration, but no difference by supplementation periods was observed. Selenium contents in hind legs significantly (P<0.05) increased with increasing supplementation periods, and also they were significantly (P<0.001) higher for Se-SMC supplementation groups in comparison to controls. However, there was no difference for selenium contents of hind legs between three and four months supplementation. Selenium contents in livers tended to slightly increase with increasing supplementation periods with no significant difference, but they were significantly (P<0.01) higher for Se-SMC supplementation groups compared with controls within the same period. The results indicated that the optimal Se-SMC supplementation period for the selenium deposition in Hanwoo steers might be around two or three months when we considered selenium contents in hind legs and livers.

• The Journal of Internal Korean Medicine
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• v.29 no.4
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• pp.1083-1099
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• 2008

Objective : We aimed to identify the effects of Bogimakseong-bang(BGMSB) treatment on cBSA-induced MN in a mouse model. Methods : We divided 20 mice into 4 groups. The normal group (NR) had no treatment. We used cBSA to induced MN to the other 3 groups. One group (CT) was treated with cBSA (7mg/kg i.p) only. The second (BG-250) was treated with cBSA (7mg/kg i.p) and BGMSB extract (250mg/kg, p.o). The third group (BG-500) was treated with cBSA(7mg/kg i.p) and BGMSB extract (500mg/kg, p.o). After cBSA and BGMSB extract treatment for 4 weeks, proteinuria, serum albumin, total cholesterol, serum creatinine, BUN, total nucleated spleen cell number and total infiltrated kidney cell number of all groups were measured. CD3e+/CD19+ and CD4+/CD8 cells ratio of peripheral blood, kidney and spleen of all groups were analyzed. $IL-1{\beta}$ and $TNF-{\alpha}$, IL-6, IgG, IgM, and $IFN-{\gamma}$ levels of all groups were gauged. Histological analysis of kidney tissue and immunohistochemical staining (CD4 CD8) of kidneys were observed. Results : Proteinuria significantly decreased and serum albumin increased in groups treated with cBSA and BGMSB extract compared with the control. Total cholesterol decreased but not significantly. CD3e+/CD19cells ratio of peripheral blood decreased. CD3e+/CD19+ and CD4+/CD8 cells percentage of kidney and spleen showed no significant change. Level of $IL-1{\beta}$, $TNF-{\alpha}$ and IL-6 significantly decreased. and $IFN-{\gamma}$ increased but has not significantly. Concentration of IgG and IgM significantly decreased compared with control. Thickness of GBM decreased on histological analysis of kidney. Deposition of CD4 and CD8 decreased on immunohistochemical staining of kidney. Conclusions : According to the above result, BGMSB had a significant effect for treating MN which is cBSA-induced.

• Journal of Ginseng Research
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• v.35 no.3
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• pp.360-367
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• 2011

In this study, we investigated the effects of total ginseng saponin (TGS) on the cutaneous wound healing process using histological analysis. A total of 24 ICR mice, 5-weeks-old, were used for all in vivo experiments. Mice were divided into control and TGS-treated groups and four equidistant 1-cm full-thickness dorsal incisional wounds were created. The wounds were extracted at days 1, 3, 5, and 7 post-injury for histomorphometrical analysis including wound area and contracture measurements, keratinocyte migration rate, and calculation of infiltrating inflammatory cells. The results showed that the wound area was smaller and keratinocyte migration rate was higher in the TGS-treated group than that of the control group from days 3 to 7. Inflammatory cells in the TGS-treated group at days 1 and 3 were reduced compared to the control group. Wound contraction in the TGS-treated group was greater than in the control group on days 3 to 5, and collagen deposition in the TGS-treated group was higher than in the control group during wound healing. The results indicate a beneficial effect of TGS when used to treat skin wounds.

• The Journal of Korean Medicine
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• v.29 no.4
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• pp.68-82
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• 2008

Objective: The purpose of our study was to show the effects of Hwalhyulmaksung-bang (Huoxiemoxing-fang, HHMSB) treatment on cBSA-induced in MN Mouse Model. Methods: We divided the 20 mice into 4 groups. One group, named NR, was not treated. The second group, named CT, was treated with cBSA (7mg/kg i.p) only. The third group, named HH-250, was treated with cBSA (7mg/kg i.p) and HHMSB extract (250mg/kg, p.o). The fourth group, named HH-500, was treated with cBSA (7mg/kg i.p) and HHMSB extract (500mg/kg, p.o). 4 weeks after cBSA, proteinuria, serum albumin, total cholesterol, serum creatinine, BUN, total cell number of spleen and kidney of all groups were measured. CD3e+/CD19+ and CD4+/CD8 cells ratio of peripheral blood, kidney and spleen of all groups were analyzed. $IL-1{\beta}$ and TNF-$\alpha$, IL-6, IgG, IgM, and IFN-$\gamma$ levels of all groups were gauged. Histological analysis of kidney tissue and immunohistochemical staining (CD4, CD8) of kidney were observed. Results: The level of proteinuria significantly decreased and serum albumin increased in the group treated with cBSA and HHMSB extract compared with the control. Total cholesterol decreased but not significantly. CD3e+/CD19cells ratio of peripheral blood is decreased, but CD4+/CD8cells ratio has no significancy. CD3e+/CD19+ and CD4+/CD8 cells percentage of kidney and spleen has no significancy. Level of $IL-1{\beta}$, IL-6 is significantly decreased, and IFN-$\gamma$ is significantly increased on HHMSB compared with control. Total IgG level significantly decreased on HHMSB compared with the control. Thickness of GBM decreased on histological analysis of kidney. Deposition of CD4 and CD8 decreased on immunohistochemical staining of kidney. Conclusions: We conclude that Hwalhyulmaksung-bang treatment may could be a useful remedy agents for treating Membranous Neuropathy(MN) induced by cationized bovine serum albumin.

• Korean Journal of Environmental Agriculture
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• v.28 no.1
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• pp.15-19
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• 2009

Ammonia in atmosphere has a negative effect on the natural ecosystems, such as soil acidification and eutrophication, by wet and dry deposition. Livestock manure, compost, and fertilizer applications to arable land have been recognised as a major source of atmospheric ammonia emissions. The objective of this study was to evaluate the efficiency of compost application techniques in reducing ammonia loss in upland soil. The reductions in ammonia emission were 70 and 15% for immediate rotary after application (IRA) and rotary at 3 day after application (RA-3d) in comparison with surface application (SA). Total ammonia emissions for 13 days, expressed as % ammonia-N applied with compost, were 42, 35.7, and 12.7% for SA, RA-3d, and IRA treatments, respectively. The ammonia emission rate fell rapidly 6 h after application and 61 % of total ammonia emission occurred within the first 24 h following surface application. The lime application along with compost significantly enhanced the total ammonia emission. Total ammonia emission for 22 days were 40.1, 31.4, and 27.7 kg/ha for immediate incorporation in soil after lime and compost application, lime incorporation in soil following 3 days after compost surface application, and compost incorporation in soil following 3 days after lime surface application, respectively. Therefore, lime and livestock manure compost application at the same time was not recommended for abatement of ammonia emission in upland soil.

• Journal of Wetlands Research
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• v.13 no.2
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• pp.329-341
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• 2011

This study was conducted with the national river, Munsancheon, which is located in Paju-si, Gyeonggi-do. The sediment discharge of Munsancheon was directly measured to analyze the sediment characteristics, and the results were used in the numerical model to predict the long-term river bed variation. The flow-total sediment discharge relation was derived using the measured total sediment discharge, and the results were compared with the total sediment discharge that was calculated using the existing prediction formula to derive a proper sediment discharge prediction method. In the actual measurements, the total annual sediment discharge was 5,478 ton/year, and the specific sediment discharge was 29.23 ton/$km^2$/year. The Ackers & White formula resulted in the values very close to the actual measurements. With the actual sediment discharge, geographical and hydrologic data as the input variables, HEC-6 and GSTARS models were comparatively analyzed. The test results showed that the HEC-6 model is suitable for the reliable prediction of the long-term river bed variation. Accordingly, the model was used for the long-term river bed variation prediction in this study. In the case of Munsancheon, deposition was continued in the downstream area and erosion occurred in the upstream area on the whole. It was expected that the stream would be stabilized in the river bed condition of 20 years later. The river bed variation was within 1 m, which was at the significance level. In the downstream area that is influenced by tide, however, the accumulation was continuously increasing within the section 2,000-7,000 m from the outlet. It seems that this should be considered in establishing the river management plans.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.1
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• pp.46-52
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• 2009

Deposition of atmospheric N that is depleted in $^{15}N$ has shown to decrease N isotope ratio ($^{15}N/^{14}N$,expressed as ${\delta}^{15}N$) of forest samples such as tree rings, foliage, and total soil-N. However, its effect on ${\delta}^{15}N$ of mineral soil-N which is biologically active N pool has never been tested. In this study, ${\delta}^{15}N$ of mineral N($NH{_4}^+$ and $NO_3{^-}$) in forest soils from organic and two depths of mineral soil layers (0 to 20 cm and 20 to 40cm depth) of Pinus densiflora stands located at two distinct areas (rural and industrial areas) in southern Korea was analyzed to investigate if there is any difference in ${\delta}^{15}N$ of mineral N between these areas. We also evaluated potential N loss of the study sites using ${\delta}^{15}N$ of mineral N. Across the soil layers, the ${\delta}^{15}N$ of $NH{_4}^+$ ranged from +8.9 to +24.8‰ in the rural area and from +4.4 to +13.8‰ in the industrial area. Soils from organic layer (+4.4‰) and mineral layer between 0 and 20 cm (+13.8‰) of industrial area showed significantly lower ${\delta}^{15}N$ of $NH{_4}^+$ than those of rural area (+8.9 and +24.3‰, respectively), probably indicating the greater contribution of $^{15}N$-depleted $NH{_4}^+$ from atmospheric deposition to forest in the industrial area than in the rural area. Meanwhile, ${\delta}^{15}N$ of $NO_3{^-}$ was not different between the rural and industrial areas, probably because ${\delta}^{15}N$ of $NO_3{^-}$ is more likely to be altered by the N loss that causes $^{15}N$ enrichment of the remaining soil N pool. Compared with the ${\delta}^{15}N$ of soil mineral N reported by other studies (from -10.9 to +15.6‰ for $NH{_4}^+$ and -14.8 to +5.6‰ for $NO_3{^-}$), the ${\delta}^{15}N$ observed in our study was substantially high, suggesting that the study sites are more subject to the N loss. It was concluded that $NH{_4}^+$ rather than $NO_3{^-}$ can conserve the ${\delta}^{15}N$ signature of atmospheric N deposition in forest ecosystems.