• Journal of Nutrition and Health
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• 제27권1호
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• pp.79-89
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• 1994

The purpose of this study was to investigate the relationship between body fat and risk factors of chronic diseases in obese children and eventually to contribute to the prevention and treatment of childhood obesity. Anthropometric parameters such as height, weight, skinfold thickness, circumferences, body fat content were measured in 55 obese subjects of age 5-20. Blood pressure, serum components, daily food intakes were also considered. The average age of the subjects was 12.5$\pm$3.3yrs and the mean obesity index was 64.7$\pm$21.3%. The average percentage of body fat was 35.6$\pm$5.4% and the mean weight of lean body mass was 45.3$\pm$13.9kg. Mean total muscle weight of the subjects was estimated to be 34.11$\pm$1.3kg. The mean systolic and diastolic blood pressures of the obese were 134.8$\pm$15.8mmHg and 69.6$\pm$11.6mgHg respectively. The concentrations fo serum triglyceride, total cholersterol, HDL-cholesterol, LDL-cholesterol were estimated to be 246.0$\pm$136.4mg/dl, 257.0$\pm$54.3mg/dl, 48.2$\pm$13.1mg/dl and 158.9$\pm$53.5mg/이 respectively. The concentrations of serum GOT and GPT were 34.6$\pm$17.1U/L and 24.7$\pm$15.3U/L. The mean content of uric acid is serum was 6.2$\pm$1.9mg/dl. Of these 55 children, 82% has hyperlipidemia, 26% was abnormal liver function and 24% was hyperuricemia. One patient was diabetic. Eighty seven% of them have developed more than one complications.

• Applied Biological Chemistry
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• 제34권2호
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• pp.142-148
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• 1991

개화후 15일된 대두미숙종자를 배양하였을 때 callus의 형성에 적당한 온도는 $24{\sim}27^{\circ}C$였으며, NAA를 생장조절제로 사용하였을 때 embryogenic callus(EC)가 많이 형성되었다. 또한 EC를 계대배양하였을 때 BA 2mg/1 사용한 배지에서는 shoot가, IAA 2mg/1 사용한 배지에서는 root가 많이 형성되었으나, BA 2mg/1 IAA 2mg/1를 동시에 사용한 배지에서는 callus의 형성만 왕성할 뿐, 기관분화의 기미는 보이지 않았다. 배양체에 함유되어 있는 total lipid는 온도가 낮은 배양조건일수록 많았으며, callus의 생육이 왕성한 $24{\sim}27^{\circ}C$에서 glycolipid, phospholipid, free sterol의 함량이 다소 많아졌으며, embryogenic structure가 많이 생겨날 때에는 free sterol의 함량이 많았다. 배양체의 지방산 조성은 EC에서 불포화 지방산의 함량이 많았으며, 불포화도는 0.837이었다. 또한 sterol조성에서도 EC에서 cholesterol의 함량이 다른 배양체에서보다 현저히 높았다.

• 동의생리병리학회지
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• 제20권6호
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• pp.1561-1566
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• 2006

Sebum is secreted due to the effect of androgen, which start to be secreted at puberty. Androgens have profound effects on the physiology of the sebaceous gland, Using the human sebocyte cell line SZ95, we investigated the inhibitory effect of Chelidonii Harba (CH) on the subum production. Our results showed that numerous cytoplasmic lipid droplets were examined by Oil red staining and lipid droplets were increased markedly by testosterone. Cell viability was dose-dependently decreased by CH as compared with untreated cells, while total lipid content and cholesterol slightly were increased by CH. Testosterone significantly stimulated the synthesis of total lipid and the synthesis of specific sebaceous lipids such as cholesterol and triglyceride. Combined treatment with CH and testosterone resulted in a lower lipid synthesis than with testosterone alone. Especially cholesteol content was reduced by combined treatment with CH and testosterone. These results indicate that CH inhibits the testosterone-induced lipid synthesis in SZ95 cells and acts antagonistically to androgen at the cellular level.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.491-495
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• 1993

Adult male and female Setaria cervi worms were implanted in the peritoneal cavity of rabbits to develop microfilaraemia. These infected rabbits revealed non-significant rise in total protein, significant reduction in albumin content and albumin-globulin ratio and significant increase in globulin and total bilirubin. The levels of total cholesterol, blood urea nitrogen and urea in blood were elevated from day 20 to 40, 25 to 70 and 30 to 70 respectively whereas, uric acid remained high from day 25 to 50 and creatinine from 15th day to 50th day of worm implantation. Biochemical changes suggested the involvement of liver and kidney in the infected rabbits.

• 한국식품저장유통학회지
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• 제13권6호
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• pp.754-761
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• 2006

본 연구는 chitosan-ascorbate (CA) 첨가(0.1%)가 물김치의 숙성에 미치는 영향과 고콜레스테롤식이 흰쥐의 혈청지질에 미치는 효과를 조사하였다. CA첨가 물김치는 무첨가에 비하여 탁도가 낮고, 보존성이 높으며, glucosamine함량이 무첨가에 비하여 $1.84{\sim}2.90$배, SOD활성이 $59.00{\sim}64.24%$로 높았으며 2025 kDa의 chitosan으로 제조한 CA2025가 가장 양호하였다. 동물실험은 SD계 흰쥐로 정상식 이군(NC), 고콜레스테롤 식이군(HC), 고콜레스테롤 식이 김치국물 투여군(HC-KC), 고콜레스테롤 식이 CA 함유 김치국물 투여군(HC-CA2025), 고콜레스테롤 식이 CA2025 1/2농축액 투여군(HC-2CA2025)의 5개군으로 구분하여 5주간 사육하였다. 그 결과, HC-CA2025군과 HC-2CA2025군의 평균체중은 HC군에 비하여 각각 6.9%, 및 8.4%가 감소되었다. 혈청 총 cholesterol의 함량은 HC-CA2025 및 HC-2CA2025군이 각각 88.33 및 85.00 mg/dL로 HC군보다 17.45 및 20.56%가 감소되었다. HC-CA2025 및 HC-2CA2025군의 HDL-cholesterol 함량은 HC군에 비하여는 각각 7% 및 23%가 증가된 반면 LDL-cholesterol 함량은 각각 13% 및 26%가 감소되었고 동맥경화지수도 정상군에는 미치지는 못하나 현저하게 감소되었다. AST 및 ALT활성은 HC군에서 증가하는 경향을 보였으나, HC-KC, HC-CA2025, 및 HC-2CA205 군에서는 낮은 활성을 나타내었다. 따라서 CA2025는 물김치의 보존성과 품질을 향상시킬 뿐만 아니라 혈중지질 개선과 동맥경화지수를 떨어트리는 효과가 있어 산업적 활용성이 기대된다.

• 대한한방소아과학회지
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• 제16권1호
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• pp.81-103
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• 2002

This present study was carried out to investigate the effect of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis in streptozotocin induced hyperglycemic rats. Body weigh, serum levels of glucose, insulin, total cholesterol, triglyceride and urine levels of volume, glucose, protein were measured in streptozotocin induced hyperglycemic rats orally receiving extracts of Sanengjingamrotang and Saengjingamrotang plus Radix Trichosanthis for 4 days. The results were as follows: 1. The change of body weight, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable increase, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 2. The change of the content on serum glucose, the medication group of Saengjingamrotang conctntration have notable decrease, as compared to control group. 3. The change of the content on serum insulin, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable increase, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 4. The change of the content on serum total cholesterol, the medication group of saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, as compared to control group. 5. The change of the content on serum triglyceride, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis Concentration have notable decrease, as compared to control group. 6. The change of urine volume, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable decrease, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 7. The change of the content on urine glucose, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, as compared to control group. 8. The Change of the content of serum protein, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, s compared to control group. 9. The change of body weight, serum insulin and urine volume, the medication group of saenfjingamrotang plus Radix Trichosanthis concentration has more notable change than the medication group of Saengjingamrotang concentration, as compared to control group. According to above mentioned results, Saengjingamrotang and Saenfjingamrotang plus Radix Trichosanthis have the effect of decreased blood sugar, serum lipid levels and urine volume, protein, glucose in streptozotocin induced hyperglycemic rats, and so they were expected to be appled to the treatment of diabetes mellitus.

• 생명과학회지
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• 제22권10호
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• pp.1407-1414
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• 2012

갱년기의 여성에는 여러 폐경 증후들이 나타나는데, 특히 에스트로겐의 감소로 인한 혈중의 지질 조성의 변화 등으로 심혈관계 질환의 발병율이 높아지게 된다. 이러한 폐경기의 문제를 감소시키기 위하여 호르몬 보충요법이 사용되고 있으나 유방암, 관상동맥질환 등을 오히려 증가시키는 등의 보고가 있다. 따라서 해조류 중에서 갈조류인 대황 추출물을 이용하여 갱년기 유도 흰쥐의 혈중지질 농도, 혈소판 응집력 및 혈액 유동성을 통하여 그 효과를 검토하고자 하였다. 또한 동물실험에 이용하기 위한 추출물 제조 방법으로는 열수와 에탄올을 이용하는 것이 일반적이므로, 이러한 추출 방법으로 추출물을 제조한 후 항산화 활성을 측정하여 활성이 우수한 추출물을 본 실험에 이용하고자 하였다. 실험 결과 갱년기 유도로 인하여 증가된 혈청 중 중성지방 및 총콜레스테롤 함량은 대황 추출물 투여에 의해 저하되었으며, 특히 대황 추출물 200 mg/kg bw/day 투여는 중성지방 함량을 유의적으로 감소시키는 결과를 나타내었다. AI와 CRF는 난소를 절제한 OVX-CON군이 난소를 절제하지 않은 SHAM군 보다 유의적으로 증가하는 결과가 나타났으나, 대황 추출물의 투여에 의해 유의적으로 감소하였다. 또한 대황추출물의 항 혈전능에 미치는 영향을 검토하기 위해, 난소절제로 인하여 혈 중 지질 함량이 증가한 상태에서 washed platelet을 분리하고 콜라겐 2 ${\mu}g$으로 혈소판 응집을 유도하였다. 그 결과 대황 추출물을 투여한 군이 OVX-CON군에 비해 혈소판 응집을 억제하는 것으로 나타났다(Table 5). Amplitude (%)는 난소절제를 한 OVX-CON군이 난소절제를 하지 않은 SHAM군과 비교하여 증가하였으나, 대황 추출물 투여군인 OVX-EB50 및 OVX-EB200군 모두에서 감소하는 경향을 나타내었다. 난소절제로 인해 OVX-CON군이 비 난소절제군인 SHAM군과 비교하여 Slop이 증가하여 혈소판 응집이 촉진되었으나, 대황 추출물 투여에 의해 감소하였고 특히 OVX-EB50군에서 유의적으로 감소하여 혈소판 응집이 억제되었다. 응집시간을 나타내는 lag time (sec)은 난소절제에 의해 반응이 일어나는 시간이 짧아져, 혈소판 응집이 촉진되는 결과가 나타났다. 이에 반해 대황 추출물 투여군인 OVX-EB50 및 OVX-EB200군에서 응집시간이 지연되었으며, 특히 OVX-EB200군의 경우 정상군인 SHAM군과 같은 응집지연 효과를 보였다. 한편, 대황 추출물이 혈액의 유동성에 미치는 영향에 대한 실험 결과 난소절제에 의해 혈액의 점도가 증가하여 유동성이 저하되었으나, 대황 추출물 투여에 의해 유동성이 향상됨을 알 수 있었다. 따라서 대황 추출물이 외부자극으로 인한 혈소판 응집능이나, 점도의 증가로 인한 혈액의 유동성에 효과적인 것으로 보여진다. 따라서 이상과 같이 대황 추출물이 혈중 LDL-콜레스테롤, AI 및 CRF를 감소시키고, 혈액 유동성을 증가시키는 결과로부터 심혈관 질환의 위험율을 저하시킬 수 있는 소재가 될 것으로 기대가 되나, 앞으로 투여량의 범위 및 조절 등의 구체적인 연구가 요구된다.

• Journal of Nutrition and Health
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• 제27권10호
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• pp.967-978
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• 1994

This study was done to investigate the effect of age and dietary linolenic acid content and the linolenic acid/linoleic acid(LNA/LA) ratio on the lipid metabolism and formation of PGI2 and TXA2. The male Sprague-Dawley rats were fed 6 different with 0.2, 0.4, 0.6 of LNA/LA ratio within either 8% LNA(high LNA) or 4% LNA(low LNA) of fatty acid content for different feeding period(1, 4, 12 month). The dietary fat used were sesame oil, perilla oil, soybean oil and beef tallow. The concentration of serum total lipid, total cholesterol and HDL-C were increased with aging. Triglyceride concentration was decreased in 0.2 ratio of LNA/LA. The lipid content of liver showed similar tendency to that of serum. The ratio of PGI2/TXA2 was increased in 1 month rats and antithrombotic effect was reduced significantly with increasing age. The PGI2/TXA2 ratio was tended to be higher in diet of 0.2 and 0.4 LNA/LA ratio at high LNA level and in diet of 0.6 LNA/LA ratio at low LNA level. Especially PGI2/TXA2 ratio was increased linearly with rising LNA/LA ratio at low LNA level. It seemed that the LNA content and LNA/LA ratio had interaction to increase the antithrombotic effect bychanging TXA2 synthesis. And the dietary fatty acid related effect lowering the serum and liver lipid content, excepting triglyceride, was increased when dietary n3/n6 ratio was high(0.6) at both high and low n3 level. Therefore, it could not be recommended to consume large amount of n3 fatty acid or high ratio of n3/n6 to prevent cardiovascular diseases. These results suggested that the dietary fatty acid ratio of n3/n6 could be determined based on the n-3 content of dietary fat to reduce the risk of cardiovascular disease.

• Asian-Australasian Journal of Animal Sciences
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• 제29권10호
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• pp.1464-1469
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• 2016

This study was carried out to investigate the effect of dietary supplementation of red ginseng by-product (RGB) on the laying performance, blood biochemistry, and microbial population in laying hens. A total of 120 Hy-Line Brown laying hens (75 weeks old) were randomly allotted to 1 of 3 dietary treatments with 4 replicates per treatment. A commercial-type basal diet was prepared, and 2 additional diets were prepared by supplementing 5.0 or 10.0 g/kg of RGB to the basal diet at the expense of corn. The diets were fed to hens on an ad libitum basis for 4 weeks. There were no differences in feed intake, egg weight, and feed conversion ratio during 4 weeks of the feeding trial. However, hen-day egg production was significantly greater (p<0.05) for the RGB treatment groups than that for the basal treatment group. There were no differences in triglyceride, aspartate aminotransferase, and alanine aminotransferase during the 4-week feeding trial. However, RGB supplementation increased (p<0.05) the serum immunoglobulin G (IgG) and IgM content compared with basal treatment group. The total cholesterol was lower (p<0.05) in the RGB treatments groups than that in the basal treatment group. The intestinal Lactobacillus population was greater (p<0.05) for the RGB treatments groups than that for the basal treatment group. However, the numbers of Salmonella and Escherichia coli were not different among dietary treatments. During the entire experiment, there was no significant difference in egg quality among all the treatments. In conclusion, in addition to improving hen-day production, there were positive effects of dietary RGB supplementation on serum immunoglobulin and cholesterol levels in laying hens.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.28-42
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• 2006

Tannins are phenolic compounds that precipitate proteins and composed of a very diverse group of oligomers and polymers. Tannins are potential biological antioxidants, which are widely believed to be an important line of defense against oxidative damage and may participate in the prevention of cancer and cardiovascular disease. Persimmon(Diospyros kaki L.) has been cultivated in East Asia and is a good source of nutritional antioxidant vitamins, carotenoids and tannins. In general persimmon peel was regarded as a waste matter, although based on recent studies, the peel contains more carotenoids and polyphenols than pulp. Several investigation conducted in experimental animals have reported that dietary persimmon fruit and peel effectively lowered the levels of plasma total cholesterol and LDL-cholesterol. We conducted experiments to investigate in vitro antioxidative activities of persimmon peel powder (PP) and its soluble tannin extract (ST) and their dietary effects on productive performances and physiological responses in poultry. The PP and ST exhibited in vitro antioxidative activity in SOD - like activity model. The yolk color and eggshell color were significantly improved by the addition of PP and ST into layer diets. The contents of total cholesterol, triacylglycerol and phospholipid of liver in the groups fed diets containing PP and ST tended to be reduce as compared with those of control. With adding of PP and ST, Haugh unit was increased after 7 and 14 days of storage. In conclusion, PP and ST can be used as valuable feed additives for reducing hepatic lipid contents without harmful effects on overall productive performances and physiological responses in laying hens.