• 한국식품영양과학회지
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• 제34권1호
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• pp.36-41
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• 2005

본 연구는 고콜레스테롤을 급여한 흰쥐 에 키토산 올리고당의 첨가 수준을 달리한 식이가 흰쥐의 지방대사에 미치는 영향을 알아보고자 수행하였다. 체중이 174.7$\pm$6.3 g인 Sprague-Dawley종 수컷 흰쥐에게 고콜레스테롤혈증을 유발시키기 위하여 콜레스테롤을 식 이 무게의 0.5%를 급여하고 키토산 올리고당을 식이 무게의 1.0%와 2.0%로 하여 총 3군으로 나누어 4주간 사육하였다. 식이섭취량과 체중증가량은 식이에 의한 영향이 없었다. 혈청 중 총 콜레스테롤, 중성지방은 대조군에 비해 2.0% 키 토산 올리고당을 첨가한 군에서 유의적으로 그 농도가 크게 감소하였으며 HDL-콜레스테롤/총콜레스테롤 비는 키토산 올리고당 첨가에 의해 높아지는 경향을 보였다. 간 조직 중 TBARS 함량은 키토산 올리고당을 첨가하였을 경우 유의적으로 낮게 나타났으며 SOD와 catalase의 활성은 키토산 올리고당을 첨가한 군이 대조군에 비해 유의적으로 낮았다. 이상의 결과를 볼 때 키토산 올리고당은 체내 지방대사에 있어 총콜레스테롤과 LDL-콜레스테롤, 중성지방 및 TBARS 함량을 낮추고 HDL-콜레스테롤/총 콜레스테롤 비는 높이는 효과를 나타냈으며, 또한 생체내 고콜레스테롤에 의한 산화적 스트레스로부터 간을 보호하는 것을 알 수 있었다.

• 동의생리병리학회지
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• 제20권2호
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• pp.372-376
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• 2006

In order to study the effects of Cervi pantotrichum on hyperlipidemia, we divided the rats into three groups (normal group, control group & sample group) and perfomed the experimental research. Hyperlipidemia rat was induced by olive oil $1m{\ell}$(involved cholesterol 0.05g)/1200g for 10 days. The sample group was administerd the extract of Cervipantotrichum 10.8mg/200g for 5 days. and control group was administerd equal dose of saline. And then we measured the amount of serum total cholesterol, triglyceride, HDL-cholesterol, and phospholipid. Cervi pantotrichum showed decreasing effects on total cholesterol, LDL-cholesterol and phospholipid level, and increasing effects on HDL-cholesterol level in serum significantly. According to the above results, Cervi pantotrichum showed significant decreasing effects on hyperlipidemia, and it is considered that it is appropriate to apply for hyperlipidemia.

• Nutritional Sciences
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• 제9권2호
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• pp.74-81
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• 2006

The present study examined the effect of soy isoflavones on lipid metabolism in growing female rats. Rats were randomly assigned to three different groups and provided experimental diets for 9 weeks. The experimental groups were classified into 1) a control group, 2) a soy protein isolate group: soy (+)) group and 3) a soy protein concentration group: soy (-)) group. Diets contained either casein or one of two soy proteins with (soy (+)) or without isoflavones (soy (-)). Serum triglyceride concentration showed no significant differences among the experimental groups. Serum total cholesterol concentration was significantly lower in both the soy (+) and soy (-) groups than in the control group and LDL-cholesterol concentration was significantly lower in the soy (+). Serum HDL-cholesterol concentration was significantly higher in the control group than in the soy protein groups but the HDL-cholesterol share rate in total cholesterol tended to be lower in the control group than in the soy protein groups, insignificant as it was. Hepatic IDL receptor mRNA level was significantly increased in the soy (+) group when compared to the other two groups to be 20% higher than the control group. In conclusion, soy protein isolate, soy protein rich with isoflavones reduced serum total cholesterol and LDL-cholesterol concentration and increased hepatic IDL receptor mRNA expression in growing female rats. Therefore, it is considered that the intake of soy isoflvones during puberty can be advantageous in terms of the long-tenn control of serum lipid.

• 한국가정과학회지
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• 제7권2호
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• pp.1-11
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• 2004

The purpose of this study was designed to observe the effects of the feeding Cynanchum wilfordii extract on the improvement of the blood glucose, lipid components in the serum of dietary hyperlipidemic and streptozotocin(STZ) -induced diabetic rats(S.D. strain, ♂) fed the experimental diets for 5 weeks. Concentrations of total cholesterol, atherosclerotic index, LDL, LDL-Cholesterol, free-cholesterol. cholesteryl ester, TG, PL and blood glucose in serum were significantly higher in the cholesterol administration groups((group 2(cholesterol+water), 4(cholesterol+Cynanchum WIlfordii 3.5g% extract)) than those in the control group (group1 , basal diet+water). But the concentrations of total cholesterol. atherosclerotic index, LDL, LDL- cholesterol. free-cholesterol, cholesteryl ester, TG, PL and blood glucose in serum were remakably lower in the group 4 than those in the group 2. In the STZ(55mg/kg B.W.)-induced diabetic groups((group 3(STZ, IP.)+water), 5(STZ(IP.)+Cynanchum WIlfordii 3.5g% extract? the serum total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free-cholesterol. cholesteryl ester, TG, PL and blood glucose concentrations actions were rather lower in the group 5 than those in the group 3. In the ratio of HDL -cholesterol concentration to total cholesterol and HDL-cholesterol concentration, Cynanchum wilfordii extract administration groups were higher percentage than III the groups 2 and 3. The activities of aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase (ALP) and lactate dehydrogenase(LDH) in serum were rather lower in the Cynanchum wllfordii extract administration groups (group 4,5) than in the cholesterol diet group(group 2) and STZ-induced diabetic group (group 3). From the above research, the physiological activity substances in Cynanchum wllfordii were effective on the improvement of the blood glucose, lipid compositions in serum of dietary hyperlipidemic and STZ-induced diabetic rats. And particularly, physiological activity substance in Cynanchum wilfordii was more effective therapeutic regimen for the control of metabolic derangements in adult disease.

• Journal of Nutrition and Health
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• 제24권5호
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• pp.399-407
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• 1991

The purpose of this study was to estimate the intake of individual fatty acid and eventually to contribute to the establishment of dietary guidelines and recommendations of fatty acids for the Koreans. The subjects consisted of female college students aged 20 to 29 years old. Their dietary intake was assessed twice. in summer and in winter, by means of a 24-hour dietary recall method. Food models and other measuring tools were also used. Concentrations of serm total triglyceride, total cholesterol and HDL-& LDL-cholesterol were measured. The subjects consumed 12.3g of polyunsaturated fatty acids, 14.3g of monounsaturated fatty acids and 14.99g of saturated fatty acids per day The ratios of polyunsaturated/saturated fatty acids( P/S) and polyunsaturatedimonounsaturatedi saturated fatty acids (P/M/S) taken by the subjects were 0.8/1.0 and 0.8/l.0/l.0, respectively. The ratio of w61w3 fatty acids was found to be 8.3/l.0. All of these values seem to fall in the desirable range. The percentages of total calorie from carbohydrate. fat and protein were 59.4%, 23.4% and 17.2%, respectively. The concentrations of serum total triglyceride, total cholesterol. HDL-cholesterol and LDL-cholesterol were 59.3ms/dl, 192.7mg/dl, and 59.0mg/dl, and 121.9mg/dl., respectively.

• Journal of Preventive Medicine and Public Health
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• 제33권1호
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• pp.69-75
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• 2000

Objectives : This study was conducted to evaluate the risk factors associated with cardiovascular disease. Methods : By the questionnaire, the informations of education level, monthly income, alcohol consumption and smoking habit were obtained. Height, weight and blood Pressure(BP) were measured by a trained nurse. The level of lasting blood sugar(FBS), total cholesterol, hight-density lipoprotein(HDL) cholesterol and triglyceride were tested by enzyne method about a group of 2888 males and 1696 females ages ever 20 who had never taken the medication for hypertension. Statistical analysis, ANOVA, stepwise multiple regression, correlation analysis were peformed using SAS package program. Results : There were significant differences among age groups in systolic and diastolic blood pressure, body mass index(BMI), FBS, triglyceride and cholesterol except HDL-cholesterol. The risk factors associated with systolic and diastolic BP significantly in male were BMI, age, alcohol intake, but in female BMI, age. Smoking habit had a significant negative correlation with BP in both sex. In the group of mild hypertension ($\geq140\;mmHg\;or\geq90\;mmHg$ defined by JNC-VI) and hypertension ($\geq160\;mmHg\;or\;\geq95\;mmHg$ defined by WHO), the percent of diabetes $(FBS\geq140\;mg/dl)$, hypercholesterolemia $(\geq240mg/dl)$, and hypertriglyceridemia $(\geq200\;mg/dl)$ were significantly higher compared with normal group in male and female. BMI, and alcohol consumption had a positive correlation with FBS, total cholesterol and triglyceride. Smoking had a positive correlation with cholesterol and triglyceride but negative correlation with HDL-cholesterol in male. The educational attainment had a negative correlation with BMI, FBS, total cholesterol and triglyceride in female. Conclusions : This study suggested that drinking, smoking, and BMI and lower educational level were associated with hypertension, hyperlipidemia and diabetes, but further evaluation was needed by prospective intervention study.

• 한국식품과학회지
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• 제31권3호
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• pp.809-814
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• 1999

각 지방 식이를 달리 처리하여 물과 대두 추출물을 섭취한 흰쥐의 혈청 지질 함량에 미치는 영향을 조사하기 위해 체중 $100{\pm}10g$의 Sprague-Dawley계 수컷 흰쥐 98마리를 콜레스테롤 비투여군, 표준지방식이, 동물성 지방식이, 식물성 지방식이, 고지방식이에 각각 1% 콜레스테롤을 첨가하여 물과 대두 추출물을 달리 섭취하여 처리한 실험군을 14개군으로 분류하여 4주 동안 사육하였다. 체중 증가량은 각 지방식이를 달리 하여 물만 섭취한 군과 대두 추출물 처리를 달리하여 섭취한 군들 사이에서 대두 추출물 군이 약간 증가하였으나 유의적인 큰 차이는 없었다. 혈청 중 중성지질과 콜레스테롤 함량에서 물만 섭취한 군에 비하여 대두추출물군에서 낮은 함량을 나타냈다. HDL-, LDL-cholesterol은 고지방식이와 동물성 지방식이에서 대두콩 추출물의 투여후 혈중 지질 개선 효과가 뚜렷하게 관찰되었고, 특히 식물성 지방 식이와 대두 추출물을 같이 투여한 군은 상승 작용을 가져와 혈중 지질 증가 억제 효과가 컸다.

• 한국응용과학기술학회지
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• 제2권1호
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• pp.67-75
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• 1985

The present study was designed to compare the effect of soybean oils and corn oils on serum levels of total cholesterol, HDL-cholesterol and free cholesterol in cholesterol-fed rats. The results are as fellows: 1. The average body weight gain in soybean oil diet group was lower than that of the control group. 2. The food efficiency ratio in soybean oil diet group was lower than corn oil diet group. 3. The serum levels of total cholesterol in soybean oil diet group than that in corn oil diet group. 4. The serum levels of free cholesterol in soybean oil diet group was lower than that in corn oil diet group. 5. The serum levels of HDL-cholesterol in soybean oil diet group than that in corn oil diet group. 6. The serum levels of VLDL-, LFL-cholesterol in soybean oil diet group was lower than that in corn oil diet group.

• Nutritional Sciences
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• 제1권1호
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• pp.8-15
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• 1998

In order to independently examine the effects of long-chain saturated fatty acids and dietary cholesterol levels on plasma and hepatic cholesterol concentrations, six different diets were fed to male Mongolian gerbils (14 gerbils pes grcup) for an 8-week period. Purified diets contained 36% energy as fat (each saturated fatty acid tested comprised about 20% of the total fat energy) and 0.06% (w/w) cholesterol, corresponding to typical human consumption patterns in Western diets. Fat blends were formulated with natural fat sources. To determine the effects of different saturated fatty acids on plasma and liver cholesterol levels, four of the six diets contained constant levels of all nutrients except for the amounts of lauric acid (12 0), myristic acid (14 : 0), palmitic acid (16 0), and stearic acid (18 : 0). Dietary cholesterol effects were tested using 16 : 0-enriched diets containing 0, 0.006, and 0.06% (w/w) cholesterol. None of the plasma lipids were influenced by fatty acid treatment, including triglycerides, plasma total-, VLDL+LDL-, and HDL-cholesterol. However, hepatic esterified cholesterol concentrations were increased in the palmitic and stearic arid diet groups compared to the lauric and myristic acid diet group. The molar ratios of hepatic EC/FC were the highest in the palmitic acid diet (12.2 $\pm$0.6) and the lowest in the myristic acid diet (6.4$\pm$0.2). Dietary cholesterol significantly (p<0.001) increased the plasma total cholesterol which was due to the increase of both HDL-and VLDL+LDL-cholesterol. In the absence of dietary cholesterol and compared to other species, the gerbil exhibited a high molar ratio of hepatic EC/FC, which was funker elevated by dietary cholesterol feeding (0.06%). The results from this study indicate that hepatic cholesterol concentrations are sensitive to both low levels of dietary cholesterol and saturated fatty acid chain length and also, that plasma cholesterol concentrations are sensitive to low levels of dietary cholesterol .

• Journal of Nutrition and Health
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• 제29권8호
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• pp.881-888
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• 1996

The purpose of this study was to determine whether vitamin B6(B6) deficiency affects fuel utilization and blood cholesterol profile with exercise-training. Twenty-four rats were fed a B6 deficient(-B6) diet or a control (+B6) diet for 5 weeks and either exercised(EX) or nonexercised (NE). EX rats were exercised on treadmill(10$^{\circ}$, 0.5-0.8km/h) for 20 minutes everyday. Glucose(GLU), glycogen (GLY), protein(PRO), trglyceride(TG), free fatty acid(FFA), total cholesterl(TC), HDL-cholesterol(HDL-C) and LDL-choleterol(LDL-C) were compared in plasma(P), liver(L) and skeletal muscle(M) of rats. There was a vitamin effect on the level of P-GLU, P-TG, M-TG, L-GLY, L-PRO and an exercise effect on the level of P-PRO, P-FFA, M-PRO, L-GLY, L-TG, P-TC, P-HDL-C, P-LDL-C. Compared to +B6 rats were lower and there were no differences in P-GLU, P-FFA, P-TG. M-GLY, L-TG, P-TC and P-HDL-C. In EX group, the level of P-TG was higher and M-PRO was lower in -B6 rats. There were no differences in M-GLY, L-TG, P-TC and P-HDL-C. These results suggest that a lowered intake of vitamin B6 may impair the adaptation of animals to fuel metabolism related to a decrease of fatty acid oxidation and attenuates the exercise-traning effect on blood lipid profile.