• 제목/요약/키워드: total bacterial counts

검색결과 346건 처리시간 0.037초

마쇄홍고추 첨가가 김치의 품질 특성에 미치는 영향 (Effects of Mashed Red Pepper on the Quality Characteristics of Kimchi)

  • 황인국;김하윤;황영;유선미;정헌상;이준수;김혜영
    • 한국식품영양과학회지
    • /
    • 제40권12호
    • /
    • pp.1769-1775
    • /
    • 2011
  • 본 연구에서는 고춧가루 대비 마쇄홍고추 첨가량을(0, 25, 50, 75 및 100%) 달리하여 제조한 김치의 숙성과정 중 품질 특성을 비교 분석하였다. 김치의 일반성분은 수분함량의 경우 마쇄홍고추 첨가량이 증가할수록 86.88~88.55%로 증가하였고, 조단백질, 조지방 및 조회분 함량은 각각 2.05~1.90, 0.35~0.29 및 3.00~2.81% 감소하였다. 숙성 중 총균수 및 젖산균수 변화는 숙성 초기에 급격히 증가한 후 서서히 증가하는 경향을 보였으며, 시료 간 큰 차이를 나타내지 않았다. 김치의 pH와 총산도는 담금 직후 각각 5.67~5.88 및 0.18~0.21%에서 숙성 2일째 4.26~4.72 및 0.50~0.70%로 시료 간 차이를 보이며 급격히 변화하다가 그 후에는 변화폭이 적었다. 환원당 함량 변화는 숙성 2일째에 급격히 감소한 후 완만하게 감소하였으며, 마쇄홍고추 첨가량이 증가할수록 감소하는 경향을 나타냈다. 관능평가 결과 마쇄홍고추를 첨가한 김치는 고춧가루로 제조한 김치와 유사한 품질 특성을 보였고, 50%와 75% 마쇄홍고추 첨가 김치에서 비교적 우수한 관능 특성을 보이는 것으로 판단된다.

Urea Treated Corncobs Ensiled with or without Additives for Buffaloes: Ruminal Characteristics, Digestibility and Nitrogen Metabolism

  • Khan, M.A.;Iqbal, Z.;Sarwar, M.;Nisa, M.;Khan, M.S.;Lee, W.S.;Lee, H.J.;Kim, H.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제19권5호
    • /
    • pp.705-712
    • /
    • 2006
  • Influences of urea treated corncobs (UTC) ensiled with or without different additives on ruminal characteristics, in situ digestion kinetics, nutrient digestibility and nitrogen metabolism were examined in a $5{\times}5$ Latin square design using five ruminally cannulated buffalo bulls. Five iso-caloric and iso-nitrogenous diets were formulated to contain 30% dry matter (DM) from concentrate and 70% DM from 5% UTC ensiled without any additive (U) or with 5% enzose (EN), 5% acidified molasses (AM), 5% non-acidified molasses (NM) and 5% acidified water (AW), respectively. These diets were fed to buffalo bulls at 1.5% of their body weight daily. Ruminal $NH_3$-N concentration at 3 hours (h) post feeding was significantly higher in bulls fed U, NM and AW diets, however, at 6, 9 and 12 h post feeding it was significantly higher in bulls fed EN and AM diets. Ruminal total volatile fatty acids (VFA) and acetate concentrations were significantly higher with EM and AM diets compared with other diets at 3, 6, 9 and 12 h post feeding. Ruminal pH at 6 and 9 h post feeding was higher with EN and AM diets; however; it was notably lower with these diets at 3 h post feeding. Total ruminal bacterial and cellulolytic bacterial counts were higher in bulls fed EN and AM diets than in those fed the other diets. In situ ruminal DM and NDF degradabilities and total tract digestibilities were significantly higher with UTC ensiled with enzose and acidified molasses than those ensiled without any additive or other additives. Nitrogen balance was significantly higher in bulls fed EN and AM diets than those fed U, AW and NM diets. The UTC ensiled with enzose or acidified molasses resulted in better digestibility and N utilization than those ensiled without any additive, with non-acidified molasses and acidified water in buffaloes.

인천지역 유통 계란의 신선도 및 영양성분 함량 조사 (Investigation for freshness and nutritive components of the eggs sold in Incheon)

  • 홍성희;라도경;윤가리;정윤정;남지현;정은하;이정구;이성모
    • 한국동물위생학회지
    • /
    • 제35권2호
    • /
    • pp.119-128
    • /
    • 2012
  • This study was performed to investigate the freshness and nutritive components of eggs from Incheon area. The eggs of 64 brands collected from 7 hypermarkets and 6 brands collected from farms were examined to test the Haugh unit (HU), egg yolk index and specific gravity. HU and specific gravity were higher in farm eggs than in hypermarket eggs (P<0.05), and there was no difference in the egg yolk index. Among hypermarket eggs, HU and egg yolk index were higher in the graded eggs than in non-graded eggs (P<0.05), and specific gravity was not different between them. HU and egg yolk index of the eggs collected in March were higher than those in August (P<0.05). To monitor the bacterial contamination, total coliforms, total bacterial counts (TBC) and Salmonella enteritidis were examined. Salmonella enteritidis and total coliforms were not detected from all eggs. TBC of farm eggs were less than $10^1$ CFU/mL in all samples and TBC of hypermarket eggs were less than $10^1$ CFU/mL in 187 samples, $10^1{\sim}10^2$ CFU/mL in 3 samples and $10^3{\sim}10^4$ CFU/mL in 2 samples, respectively. Antimicrobial residues were not detected from all eggs tested. The vitamin E contents in the vitamin E enriched eggs from 6 brands hypermarket were 1.98~22.96 mg/yolk100 g ($14.04{\pm}8.81$ mg/yolk100g), and those of 5 brands among them were higher than the average of ordinary eggs. In one brand egg, vitamin E content was lower than the average of ordinary eggs. The vitamin E contents of ordinary eggs were 1.75~16.36 mg/yolk100 g (average $5.57{\pm}2.76$ mg/yolk100 g) and there were no price differences between vitamin E enriched eggs and ordinary eggs. In the contents of vitamin E, docosahexaenoic acid (DHA) and cholesterol, there were no significant differences according to the price. These results suggests that egg production, distribution, and management system should be improved for supplying fresh eggs to consumers at reasonable prices.

유통중인 파프리카, 딸기 및 토마토의 생물학적 위해요소 분포 조사 (Biological Hazard Analysis of Paprikas, Strawberries and Tomatoes in the Markets)

  • 유용만;윤영남;;차광호;이영하
    • 한국식품위생안전성학회지
    • /
    • 제24권2호
    • /
    • pp.174-181
    • /
    • 2009
  • 생활수준의 향상으로 건강과 웰빙에 대한 관심이 늘면서 즉석섭취(ready-to-eat) 농산물의 수요가 증가하고 있다. 이들 농산물중 파프리카, 딸기, 토마토 등은 우리나라의 대표적인 수출 농산물로서 안전성 확보가 중요하다. 본 연구는 대전시내 2개 백화점과 2개 대형할인점에서 유통 중인 파프리카, 딸기 및 토마토를 대상으로 총호기성균, 대장균군, 식중독 유발 세균 및 기생충의 오염 수준을 배지 배양법, multiplex PCR, 상업용키트 및 현미경으로 조사하였으며, 또한 세척방법별 세균 감소율을 평가하였다. 유통중인 파프리카, 딸기 및 토마토의 평균 총호기성균 수는 $1.3{\times}10^4{\sim}1.8{\times}10^5$CFU/g, 평균 대장균군의 수는 $1.4{\times}10^3{\sim}9.6{\times}10^3$CFU/g 범위이었으며, 세균 오염 정도는 매장별로 유의한 차이를 보이지 않았다. 총호기성균 및 대장균군의 오염 정도는 딸기에서 가장 높았으며, 딸기에서 미확인 기생충 충란이 발견되었다. 조사한 농산물에서 가장 흔히 분리되는 세균은 Enterobacter cloacae 이었으며, 식중독 유발 세균중 Staphylococcus aureus가 딸기에서 발견 되었으나 그 외의 식중독 유발 세균은 분리되지 않았다. 딸기를 대상으로 세척방법별 세균 감소율을 조사한 결과 수돗물로 5회이상 세척시 일반세균의 80%이상을 제거할 수 있었으며 야채용 세제를 사용하거나 초음파 세척시 세균 감소율이 증가하였다. 이상의 결과로 보아, 파프리카, 딸기 및 토마토의 세균 및 기생충의 오염 정도를 조사한 결과 딸기에서 가장 많은 생물학적 오염을 보였으며, 이들 농산물들의 생물학적 오염 정도는 구입 매장별로 유의한 차이를 보이지 않았다.

Evaluation of Antioxidative Activity of Various Levels of Ethanol Extracted Tomato Powder and Application to Pork Patties

  • Kim, Hyeong Sang;Chin, Koo Bok
    • 한국축산식품학회지
    • /
    • 제37권2호
    • /
    • pp.242-253
    • /
    • 2017
  • This study was performed to evaluate antioxidant activity of tomato powder extracted by various concentrations of ethanol (0, 25, 50, 75, 100%) and to evaluate the physicochemical properties and antioxidant activities of pork patties with ethanol extracted tomato (EET) powder. No differences in the contents of total of individual phenolic compounds including gallic acid and catechin, were observed among the treatments (p>0.05). Among the various concentrations, 50% and 75% EET powder showed the highest free radical scavenging and iron chelating activities (p<0.05). Lipid peroxidation was retarded in linoleic acid emulsion with the addition of 50% and 75% EET powder (0.1%). Based on the model study, five pork patties were actually manufactured; control patty, reference patty with 0.01% of butylated hydroxytoluene, patty with 1% of water extracted tomato (WET), and patties with 0.5 and 1.0% of EET. Addition of 1% WET and EET decreased pH value, and increased redness values of pork patties, as compared to the control (p<0.05). Pork patties with WET (1.0%) and EET (0.5% and 1.0%) had lower 2-thiobarbituric acid reactive substances values compared with control patties after 7 d of storage (p<0.05). Pork patties containing EET powder showed lower total bacterial and Enterobacteriaceae counts than control patties (p<0.05). In conclusion, WET and EET (50%) could be used as a natural antioxidant and antimicrobial agent in meat products.

눈꽃동충하초(Paecilomyces japonica)를 첨가한 증편의 미생물학적 품질특성 및 저장성 (Effect of Paecilomyces japonica on the Microbiological Quality and Shelf-life of Jeungpyun)

  • 박찬성;최미애;박금순
    • 한국식품조리과학회지
    • /
    • 제20권6호
    • /
    • pp.561-567
    • /
    • 2004
  • The purpose of this study was to investigate the effect of Paecilomyces japonica mycelia(PJM) on pH, titrable acidity and microbiological qualify of Jeungpyun(fermented rice cake). Jeungpyun prepared with $0\~\%$ of PJM stored at $5^{\circ}C\;and\;20^{\circ}C$ for 4 weeks and 7 days respectively. Before fermentation of Jeungpyun dough, viable cells of total bacterial counts(TBC), yeasts and lactic acid bacteria(LAB) were $6.0\~9.8\times10^6,\;5.3\~9.0\times10^6,\;5.4\~8.5\times10^6\;CFU/g$, respectively. During the fermentation of dough, viable cells of TBC, yeasts and LAB increased $0.3\~0.4$ log cycle and pH was decreased whereas acidity increased as the progress of fermentation. Total viable cells in Jeungpyun before storage were $5.0\times10^1\;CFU/g$. During storage of Jeungpyun, TBC, yeasts and LAB of control group increased 2.6, 2.4, 2.1 log cycle at $5^{\circ}C$ and 4.8, 4.6, 4.5 log cycle at $50^{\circ}C$, respectively, when reached at maximum level. Major microflora of Jeungpyun was composed of yeasts and LAB during fermentation of dough and storage at $5^{\circ}C\;and\;20^{\circ}C$. Addition of PJM, inhibited the growth of microorganisms, the changes of PH and titrable acidity of Jeungpyun during storage at both of $5^{\circ}C\;and\;20^{\circ}C$. From these results, the addition of PJM extended the shelf-life of Jeungpyun during storage at $5^{\circ}C\;and\;20^{\circ}C$.

들깻잎을 첨가한 토마토 소스의 품질특성 및 기호도 조사 (Quality Characteristics and Sensory Evaluation of Tomato Sauce with Added Perilla Leaf)

  • 김세한;공석길;박대순
    • 한국식품영양학회지
    • /
    • 제26권4호
    • /
    • pp.766-771
    • /
    • 2013
  • Tomato sauce is widely known as a health food, In this study perilla leaf was added to a tomato sauce and the resulting functional improvements were assessed. The moisture of the tomato sauce WITH 2% added perilla leaf was 89.52%, the highest observed likewise, sample pH also significantly increased with 2% perilla leaf, to the highest pH of 4.71, (p>0.001). Lightness L value of the control group was the highest at 40.70, and the redness a value and yellowness b value were highest in, the control group, at 15.77, and 17.70, respectively. The highest salinity was foung with 2% perilla leaf, 0.96, and the sugar content with 2% perilla leaf, was 9.57. The reducing sugar was highest in the control with 63.08. The change in the total bacterial counts was measured during 9 days of storage at $4^{\circ}C$, When perilla leaf was added to, the tomato sauce, no microorganisms were detected until 0~1 days of storage. After five days of storage, microorganisms were detected in the group with 2% perilla leaf. The control group ($1.8{\times}10^5$ CFU/ml) had the highest levels of microorganisms. As the amount of perilla leaf, the total viable count of microorganisms detected was less. In the acceptability test results, the color 6.9 of the 1.5% treatment group, received the highest evaluation. Flavor and taste of the 2% perilla leaf treatment group were highly appreciated at 6.1 and 6.6, respectively. The ranking of overall acceptability was 1.5%>1%>0.5%>10%>2% of perilla leaf; 2% perilla leaf was the most underrated. Based on these results, up to 1.5% perilla leaf could be added to tomato sauce and the commercial potential could be very high.

Dietary lysophospholipids supplementation inhibited the activity of lipolytic bacteria in forage with high oil diet: an in vitro study

  • Kim, Hanbeen;Kim, Byeongwoo;Cho, Seongkeun;Kwon, Inhyuk;Seo, Jakyeom
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제33권10호
    • /
    • pp.1590-1598
    • /
    • 2020
  • Objective: The objective of this study was to evaluate the effects of lysophospholipids (LPL) supplementation on rumen fermentation, degradability, and microbial diversity in forage with high oil diet in an in vitro system. Methods: Four experimental treatments were used: i) annual ryegrass (CON), ii) 93% annual ryegrass +7% corn oil on a dry matter (DM) basis (OiL), iii) OiL with a low level (0.08% of dietary DM) of LPL (LLPL), and iv) OiL with a high level (0.16% of dietary DM) of LPL (HLPL). An in vitro fermentation experiment was performed using strained rumen fluid for 48 h incubations. In vitro DM degradability (IVDMD), in vitro neutral detergent fiber degradability, pH, ammonia nitrogen (NH3-N), volatile fatty acid (VFA), and microbial diversity were estimated. Results: There was no significant change in IVDMD, pH, NH3-N, and total VFA production among treatments. The LPL supplementation significantly increased the proportion of butyrate and valerate (Linear effect [Lin], p = 0.004 and <0.001, respectively). The LPL supplementation tended to increase the total bacteria in a linear manner (p = 0.089). There were significant decreases in the relative proportions of cellulolytic (Fibrobacter succinogenes and Ruminococcus albus) and lipolytic (Anaerovibrio lipolytica and Butyrivibrio proteoclasticus) bacteria with increasing levels of LPL supplementation (Lin, p = 0.028, 0.006, 0.003, and 0.003, respectively). Conclusion: The LPL supplementation had antimicrobial effects on several cellulolytic and lipolytic bacteria, with no significant difference in nutrient degradability (DM and neutral detergent fiber) and general bacterial counts, suggesting that LPL supplementation might increase the enzymatic activity of rumen bacteria. Therefore, LPL supplementation may be more effective as an antimicrobial agent rather than as an emulsifier in the rumen.

Bacterial Inoculant Effects on Corn Silage Fermentation and Nutrient Composition

  • Jalc, D.;Laukova, Andrea;Pogany Simonova, M.;Varadyova, Z.;Homolka, P.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제22권7호
    • /
    • pp.977-983
    • /
    • 2009
  • The survival and effect of three new probiotic inoculants (Lactobacillus plantarum CCM 4000, L. fermentum LF2, and Enterococcus faecium CCM 4231) on the nutritive value and fermentation parameters of corn silage was studied under laboratory conditions. Whole corn plants (288.3 g/kg DM) were cut and ensiled at $21^{\circ}C$ for 105 days. The inoculants were applied at a concentration of $1.0{\times}10^{9}$ cfu/ml. Uninoculated silage was used as the control. The chopped corn was ensiled in 40 plastic jars (1 L) divided into four groups (4${\times}$10 per treatment). All corn silages had a low pH (below 3.55) and 83-85% of total silage acids comprised lactic acid after 105 days of ensiling. The probiotic inoculants in the corn silages affected corn silage characteristics in terms of significantly (p<0.05-0.001) higher pH, numerically lower crude protein content and ratio of lactic to acetic acid compared to control silage. However, the inoculants did not affect the concentration of total silage acids (acetic, propionic, lactic acids) as well as dry matter digestibility (IVDMD) of corn silages in vitro. In the corn silages with three probiotic inoculants, significantly (CCM 4231, CCM 4000) lower n-6/n-3 ratio of fatty acids was detected than in control silage. Significant decrease in the concentration of $C_{18:1}$, and significant increase in the concentration of $C_{18:2}$ and $C_{18:3}$ was mainly found in the corn silages inoculated with the strains E. faecium CCM 4231 and L. plantarum CCM 4000. At the end of ensiling, the inoculants were found at counts of less than 1.0 log10 cfu/g in corn silages.

밀폐용기와 지퍼백에 저장한 잣(Pinus koraiensis)의 품질변화 연구 (Quality Changes of Pine Nuts(Pinus koraiensis) Stored in Airtight Container and Zipper Bag)

  • 김성수;박지영;박수일;이윤석;김재능
    • 한국포장학회지
    • /
    • 제14권2호
    • /
    • pp.57-63
    • /
    • 2008
  • The effects of airtight containers and zipper bags on the freshness extension of pine nuts (Pinus koraiensis) were studied at 4 and $25^{\circ}C$. Changes in total microbial growth, 2-thiobarbituric acid(TBA), color, and sensory quality were evaluated during storage. Total bacterial counts of the samples continuously increased over 28 days for airtight containers and 56 days for zipper bags, and then decreased at 70 days because the lack of air could affect the inhibition of the aerobic microbial growth. However no significant differences were observed between airtight containers and zipper bags. TBA values continuously increased with storage time, but there were no significant differences between the airtight containers and zipper bags during storage. Color changes and sensory evaluation analyses showed that the data revealed similar results for both packaging systems over the storage time. Therefore, it has been concluded that airtight containers and zipper bags could be used as a good packaging system to extend the shelf life of pine nuts by controlling the microbial growth, but the packaging systems of the airtight containers and the zipper bags was not significantly affect the quality parameters for 2-thiobarbituric acid(TBA), color, and sensory quality.

  • PDF