• 제목/요약/키워드: total bacteria

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Antibiotic sensitivity and resistance of bacteria from odontogenic maxillofacial abscesses

  • Kang, Sang-Hoon;Kim, Moon-Key
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제45권6호
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    • pp.324-331
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    • 2019
  • Objectives: This study investigated the types and antibiotic sensitivity of bacteria in odontogenic abscesses. Materials and Methods: Pus specimens from 1,772 patients were collected from affected areas during incision and drainage, and bacterial cultures and antibiotic sensitivity tests were performed. The number of antibiotic-resistant bacteria was analyzed relative to the total number of bacteria that were tested for antibiotic susceptibility. Results: Bacterial cultures from 1,772 patients showed a total of 2,489 bacterial species, 2,101 gram-positive and 388 gram-negative. For penicillin G susceptibility tests, 2 out of 31 Staphylococcus aureus strains tested showed sensitivity and 29 showed resistance. For ampicillin susceptibility tests, all 11 S. aureus strains tested showed resistance. In ampicillin susceptibility tests, 46 out of 50 Klebsiella pneumoniae subsp. pneumoniae strains tested showed resistance. Conclusion: When treating odontogenic maxillofacial abscesses, it is appropriate to use antibiotics other than penicillin G and ampicillin as the first-line treatment.

간척지토양(干拓地土壤)의 수도근권(水稻根圈)에서 협생질소고정(協生窒素固定)에 관(關)한 연구(硏究) -제(第)1보(報) 수도(水稻) 및 자연생잡초(自然生雜草) 근조직내(根組織內) 협생질소고정균(協生窒素固定菌)의 분리동정(分離同定) (Associated Nitrogen Fixation in the Rhizosphere of Rice in Saline and Reclaimed Saline Paddy Soil -1. Enumeration of aerobic heterotrophic bacteria associated in histosphere of grasses and rice)

  • 이상규;서장선;고재영
    • 한국토양비료학회지
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    • 제20권1호
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    • pp.69-76
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    • 1987
  • The aerobic heterotrophic bacteria in the histosphere associated with grasses (Gramineae, Caryphyllaceae, Crucifereae) and rice cultivars in saline and reclaimed saline paddy soils were varied with species and rice cultivars. The fraction of aerobic heterotrophic $N_2$-fixing bacteria to the total aerobic heterotrophic bacteria were averaged to eighteen percent in the histosphere of grasses and rice. Acetylene reducing activity of these bacteria were ranged from 1 to 24 n mole/tube/hr. Most of the bacteria strains were predominated of hydrogen utilizing bacteria. The majority of these bacteria were closed to Pseudomonas, Azospirillum, Klebsiella and Agrobacter.

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Evaluation of Microbiological Contamination of Water Purifiers at Two Universities in Chungcheong Region

  • Jin Young Yun
    • 대한의생명과학회지
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    • 제29권4호
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    • pp.256-262
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    • 2023
  • The purpose of this study is to investigate microbial contamination in water purifiers from two universities (A and B) in Chungcheong region and to evaluate about the harmfulness of the isolated bacteria to the human. The degree of microbiological contamination of six water purifiers at university A was investigated three times from July 2018 to September 2019, and nine water purifiers at university B were investigated in 2023. The isolated bacteria were biochemically identified using an API kit and Vitek-2 system, and then the bacteria were identified to the species level using MALDI-TOF MS. In addition, the possibility of human infection of the isolated bacteria was evaluated through a literature search. In July 2018 and September 2019, the number of bacteria isolated inside the faucet was below the acceptable standard for hot water, but exceed for cold water in all water purifiers. In January and September 2019, bacteria exceeding the acceptable standards were isolated nine times from the cold water of six water purifies (a total of 12 water purifiers). Bacteria identified by MALDI-TOF MS included anaerobic bacteria (Clostridium novyi, Clostridium themopalmarium etc.), Gram-positive bacilli (Microbacterium testaceum, Arthrobacter woluwensis etc.), and Gramnegative bacilli (Acinetobacter nosocomialis, Comamonas kerstersii etc.), which are difficult identify by biochemical methods. In conclusion, bacteria exceeding the acceptable standard were isolated from the cold water of most of the water purifiers. Most of the isolated bacteria were low-pathogenic bacteria from natural environment, but opportunistic bacteria that can cause infection in humans were also isolated from some water purifiers.

실내공기 중 생물학적 오염물질에 관한 국내 연구 동향 (Trend in Study of Biological Pollutants in Indoor Air Quality in Korea)

  • 허은혜;원동환;문경환
    • 한국환경보건학회지
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    • 제38권4호
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    • pp.300-310
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    • 2012
  • Objectives: Indoor air quality problems in public spaces have recently become a very important issue in South Korea, because many spend a long time every day indoors. In this study, an extensive literature review was performed on the subject of suspended bacteria and fungi in public facilities. The objective of this study was to evaluate the extent and types of suspended bacteria and fungi in indoor air in South Korea. This study will provide direction to the research in the field of indoor air. Methods: Article reviews was conducted using a search engine and the following keywords; 'bacteria', 'total suspended bacteria', 'fungi' and 'indoor air' from published articles since 2000. Results: Most of the places of measurement were schools, kindergartens, childcare centers and general hospitals. The highest concentrations of total suspended bacteria and fungi were found in schools and kindergartens. Some public facilities exceeded the standard of 800 CFU/$m^3$. There were few studies on other suspended bacteria: Gram Negative Bacteria, Staphylococcus and Klebsiella pneumoniae. Conclusions: It appears that more studies with intensive controls are needed. It was found that schools and kindergartens were most susceptible to microbial indoor air pollution. These results may be able to suggest an indoor standard for suspended bacteria and fungi in South Korea.

어린이집 급식실 정수기의 미생물학적 오염 평가 (Prevalence of Microbiological Contamination on Water Purifiers at Lunchroom in Child Care Center)

  • 윤미혜;김중범;오혁수
    • 한국식품조리과학회지
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    • 제28권5호
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    • pp.599-604
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    • 2012
  • In this study, the prevalence of microbiological hazard on water purifiers at lunchroom in child care center was investigated. A total of 49 water purifiers and their purified cold water were sampled to test about the total aerobic bacteria, coliform bacteria, Bacillus cereus, Staphylococcus aureus, and Salmonella spp. Total aerobic bacteria was detected over 2.0 log CFU/mL in 6 out of 49 purified cold water (12.2%), ranged from 2.0 to 2.4 log CFU/mL, and the average number of total aerobic bacteria was showed to be 3.3 log CFU/drain spout. The drain spout turned out to be a major contaminant in water purifier and needs to be improved. Coliform bacteria were also detected in 7 out of 49 cold faucets (14.3%) and 7 out of 49 drain spouts (14.3%), but not detected in purified cold water. All samples were not contaminated with the pathogens tested in this study, except for B. cereus, which was contaminated on 2 out of 49 cold faucets (4.1%) and 4 out of 49 drain spouts (8.2%). All of B. cereus isolates produced enterotoxin, such as heamolysin BL enterotoxin (HBL) or non-heamolytic enterotoxin (NHE). The HBL was detected in 5 out of 6 B. cereus isolates (83.3%), including B. cereus PCF-11 and B. cereus PDS-30 isolate only produced NHE (16.7%). These results showed that the sanitary conditions of cold faucets and drain spouts should be improved promptly.

Grazing on Bacteria and Algae by Metazoans in the Lake-river Ecosystem (River Spree, Germany)

  • Kim, Hyun-Woo;Joo, Gea-Jae;Walz, Norbert
    • 생태와환경
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    • 제41권1호
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    • pp.111-115
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    • 2008
  • Direct effects of zooplankton grazing activities on the natural assemblage of bacterioplankton and algae were evaluated at monthly intervals, from June to October of 2000, in the middle part of the River Spree, Germany. We quantified bacterioplankton, algae, zooplankton abundance and measured carbon ingestion rates (CIRs) by zooplankton according to two zooplankton size classes: (i) micro zooplankton (MICZ), ranging in size from 30 to $150{\mu}m$ and including rotifers and nauplii, excluding protozoans and (ii) macrozooplankton (MACZ), larger than $150{\mu}m$ and including cladocerans and copepods. CIRs were measured using natural bacterial and algae communities in the zooplankton density manipulation experiments. Algae biomass (average${\pm}$SD: $377{\pm}306{\mu}gC\;L^{-1}$, n=5) was always higher than bacterial biomass ($36.7{\pm}9.9{\mu}gC\;L^{-1}$, n=5). Total zooplankton biomass varied from 19.8 to $137{\mu}gC\;L^{-1}$. Total mean biomass of zooplankton was $59.9{\pm}52.5{\mu}gC\;L^{-1}$ (average${\pm}$SD, n=5). Average MICZ biomass ($40.2{\pm}47.6{\mu}gC\;L^{-1}$ n=5) was nearly twofold higher than MACZ biomass ($19.6{\pm}20.6{\mu}gC\;L^{-1}$ n=5). Total zooplankton CIRs on algae (average${\pm}$SD: $56.6{\pm}26.4{\mu}gC\;L^{-1}\;day^{-1}$) were $\sim$fourfold higher than that on bacteria $(12.7{\pm}6.0{\mu}gC\;L^{-1}\;day^{-1})$. MICZ CIRs on bacteria $(7.0{\pm}2.8{\mu}gC\;L^{-1}\;day^{-1})$ and algae $(28.6{\pm}20.6{\mu}gC\;L^{-1}\;day^{-1})$ were slightly higher than MACZ CIRs. On average, MICZ accounted for 55.6 and 50.5% of total zooplankton grazing on bacteria and algae, respectively. Considering the MICZ and MACZ CIRs, the relative role of transferring carbon to higher trophic levels were nearly similar between both communities in the lake-river ecosystem.

시중에서 유통되는 가쓰오부시의 미생물학적·화학적 위해요소분석 및 안전성 평가 (Risk Analysis and Safety Assessment of Microbiological and Chemical Hazards in Katsuobushi Products Distributed in the Market)

  • 송민규;김소희;김진수;이정석;허민수;박신영
    • 한국수산과학회지
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    • 제55권4호
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    • pp.431-436
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    • 2022
  • For the safety assessment of microbiological and chemical hazards in katsuobushi, fifteen samples of katsuobushi were purchased from supermarkets. The contamination levels of total viable bacteria, coliforms, Escherichia coli, and nine pathogenic bacteria [Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens, Enterohemorrhagic E. coli (EHEC), Yersinia enterocolitica and Campylobacter jejuni/coli] were quantitatively or qualitatively assessed. Additionally, the heavy metals (total and methyl mercury) content, radioactivity (131 I, 134 Cs+ and 137 Cs) were quantitatively assessed. Microbial and chemical analyses were performed using standard methods in Korean food code. The contamination level of total viable bacteria was 2.70 (1.18-4.42) log CFU/g. Coliforms, E. coli and S. aureus were not detected in any samples. Other eight pathogenic bacteria were negative in all samples. The contamination levels of total and methyl mercury were 0.366 (0.227-0.481) and 0.120 (0.002-0.241) mg/kg, respectively. In addition, radioactivity was not detected in any samples. The results will be helpful in revitalizing domestic use and boosting exports of katsuobushi because the microbiological and chemical safety of katsuobushi has been assured. Furthermore, the results may be used as a basis for performing chemical and microbial risk assessments of katsuobushi.

의암땜 하류수역의 분변성 오염세균류의 분포 (Occurrence Of Fecal Pollution Bacteria In The Water Of Lake Eui-Am)

  • 최상;김건치
    • 한국해양학회지
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    • 제5권2호
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    • pp.59-64
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    • 1970
  • 지표를 스치며 많은 분,지류가 모여 이루어지는 각 하천수에는 적지않은 수량의 세균류가 혼입된다. 이것들은 결국 바다로 유입되어 연안 동,식물에 섭취되거나 또는 부착되어 또다시 우리의 식생활에 이입되는 순환을 거듭하고 있고, 이러한 과정에서 각종 질병의 원인이 유발되는 수가 적지 않다. 이러한 관점에서 환경수의 세균오염은 우리의 보건위생면에서, 가정용수 또는 산업용수의 이용면에서 매우 중요한 의의를 갖는 것이라고 하겠다.

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Sensory, Physicochemical and Microbiological Changes in Water-cooked Salted Duck during Storage at 4℃

  • Li, Yanliang;Yao, Dongrui;Wang, Daoying;Xu, Weimin;Zhu, Yongzhi;Jin, Bangquan
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권7호
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    • pp.960-964
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    • 2010
  • Water-cooked salted ducks were tray-packaged and stored under refrigeration ($4{\pm}1^{\circ}C$) in order to evaluate the quality changes during storage. pH, total volatile basic nitrogen (TVB-N), sensory and microbiological analysis were determined at 0, 2, 4, 6, 8, 9 and 10 days of storage. pH value and TVB-N (mg N/100 g) varied from $6.47{\pm}0.16$ to $6.69{\pm}0.10$ and from $5.90{\pm}0.93$ to $13.42{\pm}2.46$, respectively. Sensory results indicated that ducks were unacceptable at the 10th day of storage. The predominant spoilage bacteria at the end of the shelf-life were Brochothrix thermosphacta, lactic acid bacteria (LAB) and minor components were Enterobacteriaceae, members of Micrococci, yeasts and moulds. Pseudomonads were also detected. Both total bacteria and the various spoilage ones, overall, increased from the initial sampling to the final day.

젓갈류의 원료에 따른 세균학적 안전성 평가 (Evaluation of Microbiological Safety of Commercially Salt-fermented Fishery Products by Raw Materials)

  • 심길보;박큰바위;윤나영;안병규;인정진;한형구;이우진
    • 한국수산과학회지
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    • 제54권6호
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    • pp.1045-1051
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    • 2021
  • Eighty-nine different types of commercially salt-fermented fishery products comprising various raw materials were analyzed for total aerobic bacteria, number of coliform bacteria, fecal coliform, and Escherichia coli. The food-poisoning bacterial content of the samples was investigated using next-generation sequencing. The mean mass of total aerobic bacteria in Jeotgal was 6-1.8×109 CFU/g, and that in Aekjeot and Sikhae was 4-2.2×105 CFU/mL and 1.9×105-8.4×108 CFU/g, respectively. Coliform bacteria were detected in 9 (28.1%) of 32 Jeotgal samples; 15 (46.8%) of 32 seasonal Jeotgal samples; and in 5 (55.5%) of 9 Sikhae samples. Fecal coliform and E. coli were not detected in 86 of the 89 samples. Yersinia enterocolitica was detected only in Galchi jeot (salt-fermented hairtail) (1 type) and not in other Jeotgal samples. These results contribute to our knowledge regarding the bacterial stability of salt-fermented fishery products.