• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.112-116
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• 2003

An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by Self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amino (-NH$_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au Substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The Surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 10$^2$CFU per mL.

• 한국재료학회지
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• 제24권10호
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• pp.532-537
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• 2014

In this study, highly sensitive hydrogen micro gas sensors of the multi-layer and micro-heater type were designed and fabricated using the micro electro mechanical system (MEMS) process and palladium catalytic metal. The dimensions of the fabricated hydrogen gas sensor were about $5mm{\times}4mm$ and the sensing layer of palladium metal was deposited in the middle of the device. The sensing palladium films were modified to be nano-honeycomb and nano-hemisphere structures using an anodic aluminum oxide (AAO) template and nano-sized polystyrene beads, respectively. The sensitivities (Rs), which are the ratio of the relative resistance were significantly improved and reached levels of 0.783% and 1.045 % with 2,000 ppm H2 at $70^{\circ}C$ for nano-honeycomb and nano-hemisphere structured Pd films, respectively, on the other hand, the sensitivity was 0.638% for the plain Pd thin film. The improvement of sensitivities for the nano-honeycomb and nano-hemisphere structured Pd films with respect to the plain Pd-thin film was thought to be due to the nanoporous surface topographies of AAO and nano-sized polystyrene beads.

• 자원환경지질
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• 제19권4호
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• pp.283-292
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• 1986

3각형 및 4각형 요소를 사용한 유한요소법으로 임의의 2차원 비저항 구조에서 점전류원에 대한 전위분포를 구하였다. 이 모델링기술은 종래의 차분법보다 지하의 이상물질이나 지표의 지형을 모델화할 때 보다 더 유연할 뿐만 아니라 보다 더 정확한 수치해를 준다. 또한 이 방법은 해의 정확도에 별 영향없이 격자점을 줄일 수 있기 때문에 계산시간이나 기억용량면에서 경제적이다. 본 논문에서는 이러한 특징을 보여줄 몇 가지 모델에 대한 계산 결과를 소개한다.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3269-3273
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• 2012

In this study, we report a new method for the high contrast imaging of biomolecular interactions at roller printed protein surfaces using thermotropic liquid crystals (LCs). Avidin was roller printed and covalently immobilized onto the obliquely deposited gold surface that was decorated with carboxylic acid-terminated self-assembled monolayers (SAMs). The optical response of LCs on the roller printed film of avidin contrasted sharply with that on the obliquely deposited gold surface. The binding of biotin-peroxidase to the roller printed avidin was then investigated on the obliquely deposited gold substrate. LCs exhibited a non-uniform and random orientation on the roller printed area decorated with the complex of avidin and biotin-peroxidase, while LCs displayed a uniform and planar orientation on the area without roller printed proteins. The orientational transition of LCs from uniform to non-uniform state was triggered by the erasion of nanometer-scale topographies on the roller printed surface after the binding of biotin-peroxidase to the surface-immobilized avidin. The specific binding events of protein-receptor interactions were also confirmed by atomic force microscopy and ellipsometry. These results demonstrate that the roller printing of proteins on obliquely deposited gold substrates could provide a high contrast signal for imaging biomolecular interactions using LC-based sensors.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.110-110
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• 2017

Biological systems offer unique principles for the design and fabrication of engineering platforms (i.e., popularly known as "Biomimetics") for various applications in many fields. For example, the lotus leaves exhibit unique surfaces consisting of evenly distributed micro and nanostructures. These unique surfaces of lotus leaves have the ability of superhydrophobic property to avoid getting wet by the surrounding water (i.e., Lotus effect). Inspired by the surface topographies of lotus leaves, the artificial superhydrophobic surfaces were developed using various micro- and nanoengineering. Here, we propose new platforms that can control hydrophilic and hydrophobic property of surfaces by mimicking micro- and nanosurfaces of various natural leaves such as common camellia, hosta plantaginea, and lotus. Using capillary force lithography technology and polymers in combination with biomimetic design principle, the unique micro- and nanostructures mimicking natural surfaces of common camellia, hosta plantaginea, and lotus were designed and fabricated. We also demonstrated that the replicated polymeric surfaces had different hydrophilic and hydrophobic properties according to the mimicking the natural leaf surfaces, which could be used as a simple, but powerful methodology for design and fabrication of controlled hydrophilic and hydrophobic platforms for various applications in the field of agriculture and biological engineering.

• 대한조선학회논문집
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• 제42권4호
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• pp.357-367
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• 2005

A numerical method is developed for the hydroelastic response of the Very Large Floating Structure considering the sea-bottom topography. The sea-bottom effects on the hydroelastic response of the floating structure is studied. The sea-bottom topography should be considered when the floating structure is constructed near the shore. To investigate the sea-bottom effects, four different sea-bottom topographies are considered in this study. finite-element method based on the variational formulation is used in the fluid domain, The pontoon-type floating structure is modeled as the Kirchhoff plate. The mode superposition method is adopted for the hydroelastic behavior of the floating structure.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2006년도 춘계학술발표회 논문집
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• pp.469-473
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• 2006

In general, a classification process between ground data and non-ground data, which include building objects, is required prior to producing a DEM for a certain surface reconstruction from LiDAR data in which the DEM can be produced from the ground data, and certain objects like buildings can be reconstructed using non-ground data. Thus, an exact classification between ground and non-ground data from LiDAR data is the most important factor in the ground reconstruction process using LiDAR data. In particular, building objects can be largely used as digital maps, orthophotos, and urban planning regarding the object in the ground and become an essential to providing three dimensional information for certain urban areas. In this study, an entropy theory, which has been used as a standard of disorder or uncertainty for data used in the information theory, is used to apply a more objective and generalized method in the recognition and segmentation of buildings from raw LiDAR data. In particular, a method that directly uses the raw LiDAR data, which is a type of point shape vector data, without any changes, to a type of normal lattices was proposed, and the existing algorithm that segments LiDAR data into ground and non-ground data as a binarization manner was improved. In addition, this study proposes a generalized building extraction method that excludes precedent information for buildings and topographies and subsidiary materials, which have different data sources.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2005년도 학술발표회(2)
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• pp.842-845
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• 2005

A method for the numerical simulation of two-dimensional free-surface flow resulting from the propagation of regular gravity waves over topography with arbitrary bottom shape is presented. The method is based on the numerical solution of the Euler equations subject to the fully nonlinear free-surface boundary conditions and the appropriate bottom, inflow and outflow conditions using a hybrid finite-differences and spectral-method scheme. The formulation includes a boundary-fitted transformation, and is suitable for extension to incorporate large-eddy simulation (LES) and large-wave simulation (LWS) terms for turbulence and breaking wave modeling, respectively. Results are presented for the simulation of the free-surface flow over two different bottom topographies, with constant slope values of 1:10 and 1:20, two different inflow wave lengths and two different inflow wave heights. An absorption outflow zone is utilized and the results indicate minimum wave reflection from the outflow boundary. Over the bottom slope, lengths of waves in the linear regime are modified according to linear theory dispersion, while wave heights remain more or less unchanged. For waves in the nonlinear regime, wave lengths are becoming shorter, while the free surface elevation deviates from its initial sinusoidal shape.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권4호
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• pp.227-232
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• 2003

A self-assembled monolayer of protein G was fabricated to develop an immunosensor based on surface plasmon resonance (SPR), thereby improving the performance of the antibodybased biosensor through immobilizing the antibody molecules (lgG). As such, 11-mercaptoundecanoic acid (11-MUA) was adsorbed on a gold (Au) support, while the non-reactive hydrophilic surface was changed through substituting the carboxylic acid group (-COOH) in the 11-MUA molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholide (EDAC). The formation of the self-assembled protein G layer on the Au substrate and binding of the antibody and antigen were investigated using SPR spectroscopy, while the surface topographies of the fabricated thin films were analyzed using atomic force microscopy (AFM). A fabricated monoclonal antibody (Mab) layer was applied for detecting E. coli O157:H7. As a result, a linear relationship was achieved between the pathogen concentration and the SPR angle shift, plus the detection limit was enhanced up to 10$^2$ CFU/mL.

• 한국전기전자재료학회논문지
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• 제17권12호
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• pp.1296-1300
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• 2004

SnO$_2$ is one of the most suitable gas sensor materials. The microstructure and surface morphology of films must be controlled because the electrical and optical properties of SnO$_2$ films depend on these characteristics. The effects of chemical mechanical polishing(CMP) on the variation of morphology of SnO$_2$ films prepared by RF sputtering system were investigated. The commercially developed ceria-based oxide slurry, silica-based oxide slurry, and alumina-based tungsten slurry were used as CMP slurry. Non-uniformities of all slurries met stability standards of less than 5 %. Silica slurry had the highest removal rate among three different slurries, sufficient thin film topographies and suitable root mean square(RMS) values.