• Title/Summary/Keyword: top-grafting

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Effects of eggplant rootstocks on root-knot nematode(Meloidogyne arenaria, race 2)

  • Ryu, Young-Hyun;Kim, Dong-Geun
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.267-270
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    • 2011
  • Root-knot nematodes cause a significant damage on fruit yield and quality of green house growing crops. To asses the effect of eggplant rootstock, Torvum vigor', TaibyouVF' and 'Daitaro' were grafted on eggplants(Solanum melongena cv. Chookyang) and planted in root-knot nematode infested microplot in green house and compared their fruit yield, quality and plant growth with non-grafted control. Eggplant grafted with Torvum vigor had the highest fruit yield and top growth and followed by Daitaro. Non-grafted eggplant had lower yield but had higher root weight because of heavy root-knot nematode infection. Rootstock grafting in eggplant farming is a good alternative technique in root-knot nematode infested green houses without compromising fruit yield and can be applied instantly as organic farming practice.

Rooting and Seedling Growth of Hot Pepper (Capsicum annuum L.) Cuttings as Affected by Rootone Treatment and Pinching (적심 여부와 발근촉진제 처리가 고추묘의 발근 및 생장에 미치는 영향)

  • Lee, Hee-Ju;Lee, Sang-Gyu;Lee, Jung-Myung
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.393-400
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    • 2009
  • In order to find out the feasibility of producing grafted red pepper (Capsicum annuum L.) seedlings with root-removed rootstocks, rooting response and subsequent seedling growth was also evaluated. Pinching the top of pepper seedlings for cutting significantly reduced the rooting of cuttings in 10 commercial hot pepper cultivars. Normal rooting was obtained from all commercial cultivars even though the rooting response varied considerably among cultivars. Poor rooting was observed in 'Gukbo' and 'Chungyang'. Rootone treatment significantly promoted rooting in all tested cultivars and the decrease of rooting response caused by pinching could be fully recovered by rootone treatment. Among the ten commercial rootstocks developed for hot pepper grafting, 'Tantan' showed the best rooting response followed by 'Konesian Hot' and 'Wonkwang 1'. Most of these rootstocks, however, showed higher rooting response as compared to the popular commercial cultivars, 'Manit', 'Chungyang', and 'Nokkwang'. Pasting of IBA at cut surface promoted the rooting of pepper cuttings, but the effect was not quite pronounced as compared to rootone treatment.

Effect of Rootstock on Rooting and Early Yield of Stenting-propagated Cut Roses

  • Park, Yoo Gyeong;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.11-17
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    • 2015
  • The study was conducted to investigate the effect of rootstock on growth and early yield of stenting-propagated cut roses (Rosa hybrida Hort.) 'Pink Aurora' and 'Yellow King'. The scions, prepared as single-node cuttings, each with a five-leaflet leaf, were grafted onto cuttings of Rosa indica 'Major', Rosa multiflora 'Chille Wonye No. 1', Rosa multiflora 'K-1', or Rosa multiflora 'Burr' as the rootstock. The rootstock cuttings were removed of all leaves and buds before grafting. The base of scion and the top of rootstock were held together and simultaneously cut at a $45^{\circ}$ angle for ease of grafting. Scion-rootstock unions were stuck in rockwool cubes and placed on a misted glasshouse bench for rooting before being transplanted into a rockwool slabs for cultivation. Rooting was the greatest in the 'Pink Aurora' and 'Yellow King' grafted on the rootstock Rosa indica 'Major'. In 'Pink Aurora', stem length, stem diameter, five-leaflet leaves per stem, and stem fresh weight of the harvested cut flowers were not affected by the rootstock. The greatest total yield of 'Pink Aurora' was obtained in plants grafted onto the Rosa indica 'Major' rootstock. Overall growth of 'Yellow King' was the greatest in plants grafted onto Rosa multiflora 'Burr' rootstock, although total yield was not affected by the rootstock. These results suggest that Rosa indica 'Major' is the most effective rootstock not only for rooting, but also for early yield and growth for stenting propagation of these cut roses.

Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography (RP titanium cap과 rhBMP-2를 이용하여 형성된 신생골의 미세전산화단층촬영을 이용한 평가)

  • Han, Man-Seung;Jung, Seung-Gon;Kim, Bang-Sin;Yang, Ji-Woong;Kook, Min-Suk;Park, Hong-Ju;Ryu, Sun-Youl;Oh, Hee-Kyun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.36 no.6
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    • pp.466-472
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    • 2010
  • Introduction: This study examined the effect of recombinant human bone morphogenetic protein (rhBMP)-2 and $\beta$-tricalcium phosphate ($\beta$-TCP) on new bone formation in a rabbit calvarium using a rapid prototype titanium cap (RP Ti cap). Materials and Methods: Eight New Zealand white rabbits were used in this study. Hemispherical RP Ti caps (10 mm in diameter) were implanted subperiosteally on the rabbit calvaria. $\beta$-TCP was filled in the RP Ti cap in the control group, and rhBMP-2 soaked $\beta$-TCP was used in experimental group. The rabbits were sacrificed 2 and 4 weeks after the operation. The volume and pattern of newly formed bone was analyzed by micro computed tomography (CT). Results: Macroscopically, there were no abnormal findings in any of the animals. The micro CT images revealed new bone from the calvaria that expanded gradually toward the top of the titanium cap, particularly along the inner surface of the titanium cap in the experimental group at 4 weeks after grafting. There was no significant difference in new bone volume ratio between the control and experimental groups at 2 weeks after grafting. There was a statistically significant difference in the new bone volume ratio between the experimental ($14.1{\pm}1.8\;%$) and control ($7.2{\pm}1.5\;%$) groups at 4 weeks after grafting (P<0.01). Conclusion: The RP Ti cap can effectively guide new bone formation and rhBMP-2 can induce the new bone formation.

Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig

  • Kim, Hyunji;Son, Daegu;Choi, Tae Hyun;Jung, Samhyun;Kwon, Sunyoung;Kim, Junhyung;Han, Kihwan
    • Archives of Plastic Surgery
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    • v.40 no.1
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    • pp.11-18
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    • 2013
  • Background To minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type. Methods The AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After $3{\times}3cm$ full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively. Results More rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group. Conclusions These observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing.

Preparation of Poly(vinylidene fluoride)-g-poly(methacrylic acid) Composite Nanofiltration Membrane

  • Kim, Yong-Woo;Choi, Jin-Kyu;Koh, Joo-Hwan;Kim, Jong-Hak
    • Korean Membrane Journal
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    • v.9 no.1
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    • pp.57-63
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    • 2007
  • Amphiphilic graft copolymer from poly(vinylidene fluoride) (PVDF) was synthesized using atom transfer radical polymerization (ATRP) for composite nanofiltration membranes. Direct initiation of the secondary fluorinated site of PVDF facilitates grafting of tert-butyl methacrylate (tBMA). Amphiphilic PVDF-g-PMAA graft copolymer with a 51:49 wt ratio was obtained by hydrolyzing poly(tert-butyl methacrylate) (PtBMA) to poly(methacrylic acid) (PMAA). Wide angle X-ray scattering (WAXS) and differential scanning calorimetry (DSC) confirmed the decrease of crystallinity of PVDF upon graft copolymerization. Composite nanofiltration membranes were prepared from PVDF-g-PMAA as a top layer coated onto PVDF ultrafiltration (UF) support membrane. The morphology and hydrophilicity of membranes were characterized using scanning electron microscopy (SEM) and contact angle measurement. The rejections of composite membranes were 80.2% for $Na_2SO_4$ and 28.4% for NaCl, and the solution flux were 9.5 and $14.5\;L/m^2\;h$ at 1.0 MPa pressure.

Clinical Evaluation of Simultaneous Implants Placement Following Augmentation of the Maxillary Sinus with Deproteinized Bovine Bone (탈단백 우골을 이용한 상악동 거상술 후 즉시 임플란트 식립에 대한 임상적 평가)

  • Kim, Hyun-Kuk;Kim, Jin-Wook;Kim, Chin-Soo
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.33 no.3
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    • pp.249-255
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    • 2011
  • Purpose: Placement of endosseous implants in the atrophic maxilla is often restricted because of the lack of supporting bone. In this article, augmentation of the maxillary sinus floor with deproteinized bovine bone to enable insertion of endosseous implants is described. The technique is aimed at providing a cortical layer on top of the graft to ensure a reliable seal of the maxillary sinus and to achieve optimal stability of the bone graft in case of simultaneously placement of dental implants. Methods: The procedure was used in 200 patients (839 implants), using deproteinized bovine bone. The mean follow-up was 28.5 months. No inflammation of the bone grafts nor of the maxillary sinus occurred. The patients received implant supported overdentures or bone-anchored bridges. Results: The survival rate of implant restoration of this study was 97.6%. The total average of marginal bone loss in radiographs was $0.20{\pm}0.38$ mm. Insufficient primary stability, bony quality, and infection were thought to be associated factors in the failed cases. Conclusion: This study documented that deproteinized bovine bone, when used as a grafting material for augmentation of the sinus floor, may lead to proper osseointegration of a endosseous implant.

Occurrence of Apple stem grooving virus in commercial apple seedlings and analysis of its coat protein sequence

  • Han, Jae-Yeong;Park, Chan-Hwan;Seo, Eun-Yeong;Kim, Jung-Kyu;Hammond, John;Lim, Hyoun-Sub
    • Korean Journal of Agricultural Science
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    • v.43 no.1
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    • pp.21-27
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    • 2016
  • Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these 'S' isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.

Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

  • Khan, Iftikhar Ali;Akhtar, Khalid Pervaiz;Akbar, Fazal;Hassan, Ishtiaq;Amin, Imran;Saeed, Muhammad;Mansoor, Shahid
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.47-52
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    • 2016
  • Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and ${\beta}C1$ gene and insertion of 27 nt in the middle of ${\beta}C1$ ORF. This study may help in investigating molecular basis of resistance in G. arboreum.

The Content Analysis of Clothing Design part in the middle-school Textbook of Technology and Home Economics① (중학교 기술·가정① 교과서 복식디자인 분야 내용 분석)

  • Park, Eun-Hee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.19 no.3
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    • pp.49-61
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    • 2017
  • The purpose of this study was to analyze the organizational framework and contents of the clothing design section of the middle school textbook of Technology & Home-economics(1) of the revised curriculum in 2009. The scope this study lies in external characteristics such as framework, contents and the like from the Home Economics area of textbooks by seven publishers chosen from those that were authorized by the Ministry of Education, Science and Technology in 2012. The result showed that the seven textbooks have the same top Unit 'Juvenile Life' and mid-Unit 'Clothing and Self-expression' but that smaller units were different and diverse in content. In the small unit 'Clothing and Self-expression' of the clothing design section, contents are structured to be put into practice by understanding and exercising the concept; the contents are offered as design theories, clothes design, clothing, and activities/investigation. Hanbok (Korean traditional clothes) clothing was proposed in all textbooks in an order of the right way for Hanbok clothing, names of Hanbok, kinds of Hanbok and the order of wearing Hanbok. Accordingly, this study found the seven textbooks were constructed corresponding to the revised curriculum 2009 for the clothing design section of Technology & Home-economics(1). This study suggests students can build their own insight for viewing society, the country and the world as well as appreciate their own importance in establishing consciousness if they go forward in solving the various problems they face in home life by grafting practical reasoning.

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