• Title/Summary/Keyword: tooth stain

Search Result 47, Processing Time 0.029 seconds

EARLY HISTO(PATHO)LOGIC CHANGES AND RECOVERY OF TOOTH AND PERIODONTAL TISSUE IN INTRUSION OF PREMOLAR ON YOUNG ADULT DOGS (유성견의 소구치 압하시 초기 치아 및 치주 조직의 조직학적 변화와 재생에 관한 연구)

  • Ahn, Byeong-Kyo;Cha, Kyung-Suk;Lee, Jin-Woo
    • The korean journal of orthodontics
    • /
    • v.28 no.2 s.67
    • /
    • pp.297-310
    • /
    • 1998
  • The purpose of this study was to investigate the initial tissue change, to repair on the teeth & surrounding tissue under the intrusive orthodontic forces by use of elastic chain, through the microscopic findings. For this study, three young adult mongrel dogs were used, and were divied into three group : the control group was deliveried only casting crown and the experimental group 1 was equipped with energy chain during 1 week and experimental 2 group was deliveried using energy chain during 1 week and 3 weeks observation. All experimental groups and control groups were sacrificed to make the samples for microscopic findings on premolar teeth. All samples were examed and compared the histologic changes through the microscopic with H-E stain. The obtained results were as follows. 1. In hematoxylin-eosin stain of the control group, the periodontal ligament was constant width from apical third to cervical third of the root, and the periodontal fiber arrangement was horizontal or oblique in cervical third, oblique in middle and apical third of the root. 2. In Masson Trichrome stain of the control group, osteoblast and osteoclast appeared in cervical third of root, and bone resorption and new bone formation was observed in middle and apical third of the root. 3. In experimental 1, osteoclasts were increased highly, and hyperemia of blood vessels and new bone formation and bone resorption by reversal line in apical third of the root were seen. PDL width was increased apprarently from crest to apex of the root and more in apical third. 4. In experimental 2, osteoclasts and hyperemia of blood vessels were more increased than control material in apical third of the root. PDL width was increased more than control group in root apex, and was seen less than experimental 1. PDL arrangement was similar to experimental 1 and was mixed only in root apex. Therefore, in premolar intrusion of the young adult dog, there were increased osteoclast, hyperemia and dilation of blood vessel, resorption of alveolar bone and cementum and different arrangement of PDL in initial tissue change. There was not observed complete repair after remove intrusive force.

  • PDF

THE EFFECTS OF POROUS REPLAMINEFORM HYDROXYAPATITE AND DECALCIFIED FREEZE DRIED BONE ON THE REGENERATION OF THE ALVEOLAR BONE IN THE PERIODONTALLY INVOLVED EXTRACTION SOCKETS OF DOGS (Porous Replamineform Hydroxyapatite와 Decalcified Freeze Dried Bone이 치주질환 이환 발치와의 치유에 미치는 영향)

  • Son, Hyo-Sang;Cho, Kyoo-Sung;Chai, Jung-Kiu;Kim, Chong-Kwon
    • Journal of Periodontal and Implant Science
    • /
    • v.23 no.2
    • /
    • pp.315-330
    • /
    • 1993
  • The ultimate goal of periodontal treatment has been to facilitate regeneration of diseased periodontal tissues, destroyed by inflammatory periodontal disease. Various implant materials have been used to restore the alveolar bone defects. Of the various materials, porous replamineform hydroxyapatite (PHA) has good biocompatibility when placed in a bone tissue, and maintains alveolar ridge for a long period. Decalcified freeze dried bone(DFDB) has been widely used in alveolar bone defects because of its conformity and high osteogenic potential. The purpose of this study was to evaluate the effects of PHA and DFDB on the regeneration of the alveolar bone between fresh extraction sockets and periodontally involved extraction sockets. Experimental periodontitis was induced by the ligation of orthodontic elastic threads after surgically creating periodontal defects on the premolars on the right side of 2 adult dogs for 8 weeks. Following the extraction of each tooth, PHA and DFDB were inserted in the extraction sockets. In control group 1, PHA was inserted in the fresh extraction sockets, and in control group 2, DFDB was inserted. In experimental group 1, PHA was inserted in the periodontally involved extraction sockets, and in experimental group 2, DFDB was inserted. After 20 weeks, the specimens were prepared and stained with Hematoxylin-Eosin stain for the light microscopic evaluation. The results of this study were as follows. 1. No inflammation associated with implant materials was evident in any of the groups. 2. DFDB was completely resorbed, PHA was remained in the extraction sockets in the control and experimental groups. 3. In control group 1 and experimental group 1, extraction sockets were not completely filled with new bone. However, original forms of alveolar crests were maintained in control group 2 and experimental group 2. 4. In control group 1 and exprimental group 1, PHA particles surrounded with many giant cells were well tolerated by the fibrous connective tissues in the coronal part of the socket, In the inferior part of the socket, PHA particles were incorporated into the new bone. In both control group 2 and experimental group 2, DFDB was replaced by newly remodeled bone. 5. No differences of degree of new bone formation were evident between control and experimental groups.

  • PDF

EFFECT OF ELECTRICAL STIMULATION ON BONE FORMATION IN THE EXTRACTION SOCKET OF RAT (전기자극이 치조골 치유과정에 미치는 영향)

  • Yang, Seung-Han;Lee, Man-sup;Park, Joon-bong;Herr, Yeek
    • Journal of Periodontal and Implant Science
    • /
    • v.31 no.4
    • /
    • pp.669-687
    • /
    • 2001
  • On the basis of the evidence that electrical stimulation could promote healing and regeneration of bone, this study was performed to investigate the effects of electrical stimulation on rat extraction socket, and to evaluate the potential of clinical application of electrical stimulation. Forty rats were used and divided into control groups(l0)and the experimental groups(30) in this study. The maxillary 1st molar were extracted in both groups. In experimental group, electrical stimulation was given at the current intensity of lmA(Test-1), l0mA(Test-2), 25mA(Test-3) each day. At 1,3,5,7 days after the tooth extraction, rats in both groups were serially sacrificed. And the specimens were prepared with Hematoxylin-Eosin stain for the light microscopic evaluation. The results of this study were as follows; 1. At 1 day after the extraction, the periodontal ligament was found in the extraction socket wall. The formation of blood clot with dense infiltration of inflammatory cells in control group and there were less inflammatory cells in test group. 2. At 3 day after the extraction, the cells and collagen of the periodontal ligament were so actively proliferated and synthesized that invaded into the connective tissue of the extraction sockets in the control group. There were the formation of new bone in the basal & lateral portion of socket wall in test -2 and -3. 3. At 5 days after the extraction, there were no formation of new bone in control group. But the more electrical stimulation was applied, the more formation of new bone in test group. 4. At 7 days after the extraction, the extraction sockets were almost filled with trabecular bone in each group. Bone maturarity was remarkable in test-3. 5. The electrical stimulation at l0mA and 25mA was more effective in the bone formation at 5 and 7 days after the extraction. From the above results, electrical stimulation could promote the extraction socket wound healing, and be utilized in the clinical application of the residual ridge expansion.

  • PDF

Regulation of cementoblast differentiation and mineralization using conditioned media of odontoblast (상아모세포의 조건배지를 이용한 백악모세포의 분화와 석회화 조절)

  • Moon, Sang-Won;Kim, Hye-Sun;Song, Hyun-Jung;Choi, Hong-Kyu;Park, Jong-Tae;Kim, Heung-Joong;Jang, Hyun-Seon;Park, Joo-Cheol
    • Journal of Periodontal and Implant Science
    • /
    • v.36 no.2
    • /
    • pp.385-396
    • /
    • 2006
  • For the regeneration of periodontal tissues, the microenvironment for new attachment of connective tissue fibers should be provided, At this point of view, cementum formation in root surface plays a key role for this new attachment. This study was performed to figure out which factor promotes differentiation of cementoblast Considering anatomical structure of tooth, we selected the cells which may affect the differentiation of cementoblast - Ameloblast, OD11&MDPC23 for odontoblasts, NIH3T3 for fibroblsts and MG63 for osteoblasts. And OCCM30 was selected for cementoblast cell line. Then, the cell lines were cultured respectively and transferred the conditioned media to OCCM30. To evaluate the result, Alizarin red S stain was proceeded for evaluation of mineralization. The subjected mRNA genes are bone sialoprotein(BSP), alkaline phosphate(ALP) , osteocalcin(OC), type I collagen(Col I), osteonectin(SPARC ; secreted protein acidic and rich in cysteine). Expression of the gene were analysed by RT-PCR, The results were as follows: 1. For alizarin red S staining, control OCCM30 didn't show any mineralized red nodules until 14 days. But red nodules started to appear from about 4 days in MDPC-OCCM30 & OD11-OCCM30. 2. For results of RT-PCR, ESP mRNAs of control-OCCM30 and others were expressed from 14 days, but in MDPC23-OCCM30 & OD11-OCCM30 from 4 days. Like this, the gene expression of MDPC23-OCCM30 & OD11-OCCM30 were detected much earlier than others. 3. For confirmation of odontoblast effect on cementoblast, conditioned media of osteoblasts(MG63) which is mineralized by producing matrix vesicles didn't affect on the mineralized nodule formation of cementoblasts(OCCM30). This suggest the possibility that cementoblast mineralization is regulated by specific factor in dentin matrix protein rather than matrix vesicles. Therefore, we proved that the dentin/odontoblast promotes differentiation/mineralization of cementoblasts. This new approach might hole promise as diverse possibilities for the regeneration of tissues after periodontal disease.

COINCIDENCE BETWEEN RADIOGRAPHS AND CLEARING SAMPLES ON THE ROOT CANAL SYSTEMS OF SINGLE HOOTED PREMOLARS (단근 소구치의 근관계에 관한 방사선 사진과 투명표본 간의 일치도)

  • Hwang, Ho-Keel;Kang, Ho-Min;Seo, Kang
    • Restorative Dentistry and Endodontics
    • /
    • v.30 no.6
    • /
    • pp.461-469
    • /
    • 2005
  • The aim of this study was to compare the root canal systems of maxillary and mandibular premolars that had a single root using radiographs and clearing samples. 142 single rooted premolars were selected and mesio-distal and bucco-lingual views were radiographed using intra-oral dental standard films. Four equally trained examiners classify the root canal types from the developed radiographs. After opening the tooth for access, it was stored in $5\%$ NaOCl to dissolve the pulp tissue. Indian ink was then injected into the pulp cavity to stain the pulp tissue. It was cleared in methyl salicylate after being decalcified with $5\%$ nitric acid for 48 hours, and the root canal type was evaluated at a magnification of $\times$ 20 using a stereomicroscope. The results are as follows ; There were statistically significant differences between the radiographs and clearing samples of the root canal types among examiners (p < 0.05). There might be differences in the root canal types among examiners when the same radiograph is used. Therefore, considering the difficulty in estimating the root canal types, clinicians need to be careful when interpreting radiographs before root canal therapy.

Histologic changes of tooth and periodontal tissues applying to contraction & intrusion force for the maxillary four incisors of dogs (성견 상악 4절치의 Contraction과 압하시 치아 및 주위 조직의 조직학적 변화에 관한 실험 연구)

  • Kim, Young-Kuk;Cha, Kyung-Suk;Lee, Jin-Woo
    • The korean journal of orthodontics
    • /
    • v.29 no.5 s.76
    • /
    • pp.535-549
    • /
    • 1999
  • The Purposes of this study were to investigate the initial tissue changes on the teeth and surrounding tissues under contraction and intrusive force by contraction UTA. A control and experimental dogs, 10-months in age, were studied. Contraction and intrusive force(60gm) were applied at upper four incisors by contraction UTA. Experimental dogs were sacrificed at 2 weeks and 4 weeks after force application, respectively. In this study, 2 experimental groups were designed by the duration of force applied(E1, E2). The specimens were taken around the upper first and second incisor in each groups and were prepared for the H-E and MT stain for light microscopic observation. From the results of the study, the following conclusions may be drown. : 1. In control group, the periodontal ligament width was constant from apical third to cervical third of the root and periodontal fiber arrangement was horizontal or oblique in cervical third, oblique in middle third and apical third. In alveolar bond, smooth appearance was shown with osteoblast. 2. In experimental group 1, in proportion to force was concentrated at labial middle third and apical third of root of the upper first and second incisors, root of these tooth tipped labially and intruded at a time. 3. In experimental group 2, periodontal ligament width and arrangement was similar to control and observed strong calcified response at the labial middle third of root. But, alveolar bond resorption and cementum resorption were as before seen at labial middle third and apical third of root that force was concentrated.

  • PDF

The effect of various foods on the color stability of artificial teeth (여러가지 음식물에 따른 인공치의 색 안정성에 관한 연구)

  • Lee, Youngil-Il;Cho, In-Ho;Lee, Joon-Seok
    • The Journal of Korean Academy of Prosthodontics
    • /
    • v.47 no.1
    • /
    • pp.82-90
    • /
    • 2009
  • Statement of problem: Recently, as patients' expectation and interest for esthetics are increasing, concerns of esthetic restoration for removable dentures as well as fixed prosthodontics are also increasing. And the color stability of artificial teeth will affect a long term success rate of the denture. But the stain or discoloration of these artificial teeth as well as denture resin has caused esthetic problem. Purpose: This study was designed to evaluate the influence on color stability of artificial teeth when soy sauce, red pepper paste and coffee which many Koreans have eaten were applied. Material and methods: For artificial teeth type(Endura $Anterio^{(R)}$, Physio $Duracross^{(R)}$, Trubyte $Biotone^{(R)}$) selected for the study, 10 specimens each were soaked into individual beakers of soy sauce, red pepper paste, coffee and distilled water. And $L^*$, $a^*$, $b^*$ value were measured for evaluation of the color difference (${\Delta}E^*$) with spectrophotometer on the 1 day, 1 week, 2 weeks, 4 weeks and 8 weeks after immersion. Results: 1. ${\Delta}E^*$ value of artificial teeth which were soaked in soy sauce and coffee was various according to soaking periods. However there was significant difference between Trubyte $Biotone^{(R)}$ and Physio $Duracross^{(R)}$ in red pepper paste regardless of soaking period(P<.05). 2. Except for 8 weeks of Endura $Anterio^{(R)}$, 4 weeks and 8 weeks of Physio $Duracross^{(R)}$, artificial teeth soaked in red pepper paste regardless of the type had significant difference of ${\Delta}E^*$ value compared with other groups (P<.05). 3. $a^*$, $b^*$ value of Endura $Anterio^{(R)}$ and Trubyte $Biotone^{(R)}$ which were soaked in red pepper paste had significant difference compared with the value of other group(P<.05). Conclusion: Red pepper paste had the greatest effect on color difference of artificial tooth. Physio $Duracross^{(R)}$ showed relatively less color difference than Trubyte $Biotone^{(R)}$ and Endura $Anterio^{(R)}$. But as ${\Delta}E^*$ difference were all less than 3.3, these artificial resin teeth seemed appropriate for clinical use.