• Title/Summary/Keyword: tomato leaf mold

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Antifungal Activities of Bacillus thuringiensis Isolates on Barley and Cucumber Powdery Mildews

  • Choi, Gyung-Ja;Kim, Jin-Cheol;Jang, Kyoung-Soo;Lee, Dong-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.17 no.12
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    • pp.2071-2075
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    • 2007
  • Fourteen Bacillus thuringiensis isolates having both insecticidal activity and in vitro antifungal activity were selected and tested for in vivo antifungal activity against tomato late blight, wheat leaf rust, tomato gray mold, and barley powdery mildew in growth chambers. All the isolates represented more than 70% disease control efficacy against at least one of four plant diseases. Specifically, 12 isolates exhibited strong control activity against barley powdery mildew. Under glasshouse conditions, four (50-02, 52-08, 52-16, and 52-18) of the isolates also displayed potent control efficacy against cucumber powdery mildew. To our knowledge, this is the first report of B. thuringiensis isolates that have disease control efficacy against powdery mildew of barley and cucumber as well as insecticidal activity.

Occurrence of Major Diseases in Vegetable Growing under the Furnihsed Condition in Southern Part of Korea (남부 시설원예지대의 주요병 발생생태에 관한 연구)

  • Choi Jin-Sik;Park Chang-Seuk
    • Korean journal of applied entomology
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    • v.21 no.3 s.52
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    • pp.153-158
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    • 1982
  • The study was conducted to obtain the basic information on the diseases epidemics of vegetable crops grown in Namji, Jinju, Gimhae and Suncheon under the extremely varied and specified conditions, plastic film house. The disease survey was conducted from the end of April, 1951 to April 1982. Leaf mold and late blight were serious foliar disease in tomato during the seedling stage, especially when the infected seeds were sown. The diseases increased rapidly 35 days after seeding. In both continuous cultivation of cucumber and rotation with upland crops, incidence of Fusarium wilt was severe while incidence of the disease was negligible in cultivations after paddy rice or grafting on pumpkin. Downy mildew of cucumber was severe in Jinju and Suncheon area, however, it was not so serious in Namji area where the growing season of cucumber was unfavorable for the maximum disease incidence. Cucumber mosaic virus disease was prevalent in the areas surveyed and the disease incidence was increased rapidly after June. Powdery mildew prevailed at the early stage of cucumber growth after transplanting in Namji area. Root rot and wilting caused by Phytophthora capsici was as the most destructive disease in pepper grown under the vinyl house, especially in Namji and Jinju area where the pepper has been cultivated intensively. The Phytophthora attacked most parts of young plants during the winter time and then induced crown rot on the adults plants. Cultivation of pepper in vinyl house was almost impossible because of the Phytophora disease by the end of June. Virus diseases to tomato plants were prevalent throughout the surveyed area and the damage was also severe. In Jinju and Gimhae area leaf mold and late blight showed high infection rate in tomato during the harvest time.

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Synthesis of combinatorial library of $\beta$-ketoacetoanilide chlorides and their antifungal activity against main plant pathogens ($\beta$-Ketoacetoanilide 염화물의 조합 라이브러리 합성 및 주요 식물병원균에 대한 항균활성)

  • Hahn, Hoh-Gyu;Nam, Kee-Dal;Bae, Su-Yeal;Yang, Bum-Seung;Lee, Seon-Woo;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.8 no.1
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    • pp.8-15
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    • 2004
  • A synthesis of new $\beta$-ketoacetoanilide chloride derivatives and anti fungal activity of these compounds library against 6 typical plant pathogens were described. Reaction of ketene dimer with chlorine followed by treatment of aniline derivatives gave 89 kinds of the corresponding $\beta$-ketoacetoanilide chlorides through combinatorial synthetic technology using Carousel Reaction Stations. Evaluation of antifungal activity (in vivo) of this chemical library against rice blast, rice sheath blight, tomato aray mold, tomato late blight, wheat leaf rust and barley powdery mildew was carried out. In general, $\beta$-ketoacetoanilide chlorides which present a substituent at 4 in phenyl group(para) of the compounds showed selective control activity against tomato late blight caused by Phytophthora infestans.

Biocontrol Efficacy of Formulated Pseudomonas chlororaphis O6 against Plant Diseases and Root-Knot Nematodes

  • Nam, Hyo Song;Anderson, Anne J.;Kim, Young Cheol
    • The Plant Pathology Journal
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    • v.34 no.3
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    • pp.241-249
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    • 2018
  • Commercial biocontrol of microbial plant diseases and plant pests, such as nematodes, requires field-effective formulations. The isolate Pseudomonas chlororaphis O6 is a Gram-negative bacterium that controls microbial plant pathogens both directly and indirectly. This bacterium also has nematocidal activity. In this study, we report on the efficacy of a wettable powder-type formulation of P. chlororaphis O6. Culturable bacteria in the formulated product were retained at above $1{\times}10^8$ colony forming units/g after storage of the powder at $25^{\circ}C$ for six months. Foliar application of the diluted formulated product controlled leaf blight and gray mold in tomato. The product also displayed preventative and curative controls for root-knot nematode (Meloidogyne spp.) in tomato. Under laboratory conditions and for commercially grown melon, the control was at levels comparable to that of a standard commercial chemical nematicide. The results indicated that the wettable powder formulation product of P. chlororaphis O6 can be used for control of plant microbial pathogens and root-knot nematodes.

Temporal Dynamics of Triflumizole Residue in Tomato Leaves as Affected by Temperature and Its Effects on Cladosporium fulvum (토마토 잎에 집적된 Triflumizole 잔류량의 온도에 따른 경시적 동태와 잎곰팡이병균에 대한 약호)

  • 오연이;박은우;조일규;강창성;김성기;양장석
    • Korean Journal Plant Pathology
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    • v.12 no.3
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    • pp.307-315
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    • 1996
  • 토마토 잎에 집적된 triflumizole 잔류량 동태에 미치는 온도 영향과 Cladosporium fulvum에 대한 triflumizole의 살균효과를 구명하였다. 8주간 키운 토마토 식물체에 triflumizole을 분무 살포한 후 식물생장용 항온실에서 키우면서 4가지 온도 조건을 처리하였다. 주기적으로 토마토 잎을 채취하여 HPLC 방법을 이용하여 triflumizole의 잔류량을 조사하였다. Triflumizole 잔류량의 경시적 동태는 약제살포후 적산온도를 독립변수로 사용하는 지수적 감소 몸형으로 나타낼수 있었으며, 이는 triflumizole의 잔류성이 온도 영향을 크게 받는다는 것을 뜻한다. 경기도 농촌진흥원에 위치한 토마토 비닐하우스에서 수행한 실험에서 얻은 자료를 이용하여 평가한 결과 이 모형의 타당성이 인정되었다. Triflumizole의 약효에 대한 in vitro 실험 결과, 이 살균제는 C. fulvum의 균사 생장과 포자형성을 뚜렷이 억제하였으며, 잎곰팡이병 방제를 위해서는 토마토 잎에 집적된 triflumizole 잔류량이 최소한 10 ppm 이상이 되어야 할 것으로 추측된다. 본 연구에서 연구된 지수적 감소 모형은 토마토 잎에 집적된 triflumizole 잔류량을 추정하는데 활용될 수 있으며, 실용적인 측면에서 볼 때, 토마토 잎곰팡이병을 효과적으로 방제하는데 필요한 triflumizole의 최소 잔류량을 식물체에 유지하기 위하여 triflumizole을 살포해야 할 시기를 결정하는데 활용될 수 있다.

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Newly Recorded Problematic Plant Diseases in Korea and Their Causal Pathogens

  • Kwon, Jin-Hyeuk
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.25-27
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    • 2003
  • Since 1993, a total of 50 problematic plant diseases unrecorded in Korea were surveyed in Gyeongnam province. Totally 34 new host plants to corresponding pathogens investigated in this study were 5 fruit trees, 9 vegetables, 12 ornamental plants, 3 industrial crops, and 5 medicinal plants. Among the newly recorded fruit tree diseases, fruit rot of pomegranate caused by Coniella granati and Rhizopus soft rot of peach caused by Rhizopus nigricans damaged severely showing 65.5% and 82.4% infection rate. Among the vegetable diseases, corynespora leaf spot of pepper caused by Corynespora cassiicola and the crown gall of pepper caused by Agrobacterium tumefaciens, powdery mildew of tomato caused by Oidiopsis taurica were the most severe revealing 47.6%, 84.7%, and 54.5% infection rate in heavily infected fields, respectively. In ornamental plants, collar rot of lily caused by Sclerotium rolfsii, gray mold of primula caused by Botrytis cinerea, soot leaf blight of dendrobium caused by Pseudocercospora dendrobium, sclerotinia rot of obedient plant caused by Sclerotinia sclerotiorum showed 32.7 to 64.8% disease incidence. On three industrial plants such as sword bean, broad bean, and cowpea, eight diseases were firstly found in this study. Among the diseases occurring on broad bean, rust caused by Uromyces viciae-fabae and red spot caused by Botrytis fabae were the major limiting factor for the cultivation of the plant showing over 64% infection rate in fields. In medicinal plants, anthracnose of safflower caused by Collectotrichum acutatum was considered the most severe disease on the plant and followed by collar rot caused by Sclerotium rolfsii.(중략)

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In vivo Antifungal Activity Against Various Plant Pathogenic Fungi of Curcuminoids Isolated from the Rhizomes of Curcuma longa

  • Cho, Jun-Young;Choi, Gyung-Ja;Lee, Seon-Woo;Lim, He-Kyoung;Jang, Kyung-Soo;Lim, Chi-Hwan;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.94-96
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    • 2006
  • In a search for plant extracts with potent in vivo antifungal activity against various plant pathogenic fungi, the methanol extract of the Curcuma longa rhizomes effectively controlled the development of rice blast catised by Magnaporthe grisea and tomato late blight caused by Phytophthora infestans. Three curcuminoids such as curcumin, demethoxycurcumin, and bisdemethoxycurcumin were purified from the methanol extract of C. longa rhizomes as antifungal principles. Among the three curcuminoids, demethoxycurcumin was the most active to both rice blast and tomato late blight, followed in order by curcumin and bisdemethoxycurcumin. However, they all exhibited no or little in vivo antifungal activity against other fungal pathogens causing rice sheath blight (Corticium sasaki), tomato gray mold (Botrytis cinerea), wheat leaf rust (Puccinia recondita), or barley powdery mildew (Blumeria graminis f. sp. hordel).

Screening for Antifungal Endophytic Fungi Against Six Plant Pathogenic Fungi

  • Park, Joong-Hyeop;Park, Ji-Hyun;Choi, Gyung-Ja;Lee, Seon-Woo;Jang, Kyoung-Soo;Choi, Yong-Ho;Cho, Kwang-Yun;Kim, Jin-Cheol
    • Mycobiology
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    • v.31 no.3
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    • pp.179-182
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    • 2003
  • A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 11 locations in Korea. Their antifungal activities were screened in vivo by antifungal bioassays after they were cultured in potato dextrose broth and rice solid media. Antifungal activity against plant pathogenic fungi such as Magnaporthe grisea(rice blast), Corticium sasaki(rice sheath blight), Botrytis cinerea(tomato gray mold), Phytophthora infestans(tomato late blight), Puccinia recondita(wheat leaf rust), and Blumeria graminis f. sp. hordei(barley powdery mildew) was determined in vivo by observing the inhibition of plant disease development. Twenty(11.7%) endophytic fungi fermentation broths were able to control, by more than 90%, at least one of the six plant diseases tested. Among 187 liquid broths, the F0010 strain isolated from Abies holophylla had the most potent disease control activity; it showed control values of more than 90% against five plant diseases, except for tomato late blight. On the other hand, fourteen(7.5%) solid culture extracts exhibited potent disease control values of more than 90% against one of six plant diseases. The screening results of this study strongly suggested that metabolites of plant endophytic fungi could be good potential sources for screening programs of bioactive natural products.