• 한국발생생물학회지:발생과생식
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• 제16권2호
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• pp.113-120
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• 2012

정상 발정주기를 보이는 7주령부터 38주령의 생쥐를 대상으로 발정주기에 따른 자궁조직내 비만세포의 분포를 toluidine blue 염색법으로 조사하였다. 비만세포의 밀도는 생쥐의 연령 증가와 더불어 지속적으로 증가하다가, 30주령 이후 감소하는 경향을 보였다. 발정주기별 분포에서는 조사한 모든 연령의 생쥐에서 발정후기에 가장 높은 밀도를 나타내었고, 그 대부분은 자궁근층에서 발견되었다. 10주령의 생쥐를 대상으로 Alcian blue-safranin 이중염색법에 따라 비만세포의 유형별 상대분포를 조사한 실험에서는 발정주기의 전 시기에 걸쳐 점막형 비만세포의 비율이 결합조직형 및 혼합형 비만세포의 비율에 비해 상대적으로 현저히 높게 나타났으나, 발정기 이후 발정간기까지는 점막형비만세포의 상대 비율은 다소 감소하고, 다른 유형의 비만세포, 특히 혼합형 비만세포의 비율이 상대적으로 증가하는 경향을 보였다. Masson trichrome 염색법으로 조사한 교원섬유의 분포 양상은 비만세포의 분포 양상과 정확히 일치하게 발정후기에서 가장 높은 밀도를 나타내었다. 이상의 결과는 비만세포, 특히 점막형 비만세포가 교원섬유와 함께 발정주기에 따른 자궁의 점막 및 근육조직의 재구성에 중요한 역할을 하고 있음을 시사한다.

• 대한수의학회지
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• 제32권4호
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• pp.463-479
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• 1992

The morphological findings of the parotid, mandibualr and sublingual salivary glands of the Korean native goat have been investigated by the histological and histochemical observation using the light microscope. Tissues were fixed with 10% neutral buffered formalin and Bouin's solution, and embedded in paraffin. The tissue sections were stained with hematoxylin and eosin, Heidenhain's azocarmine-aniline blue, alcian blue, toluidine blue, periodic acid Schiff, aldehyde fuchsin, alcian blue-periodic acid Schiff and aldehyde fuchsin-alcian blue. Some sections were stained with the alcian blue after each teatment of diastase digestion, methylation, methylation-saponification, and neuraminidase digestion. The results were as follows ; 1. The major salivary glands were compound. tubuloacinar glands, and the parenchyma was composed of acini, intercalated ducts, striated ducts and excretory ducts. 2. The acini were composed of serous cells in the parotid gland, and mucous cells, serous cells and seous demilunes in the mandibular gland. The acini of the sublingual glands were composed of mucous cells and serous demilunes. 3. In histochemistry, the serous cells of the parotid gland contained neutral mucin and enzyme-liable silaic acid. 4. The serous cells and demilunes of the mandibular gland contained neutral mucin and enzyme-liable sialic acid, and the mueous cells contained sulfated mucin, enzyme liable sialic acid and neutral mucin 5. In the sublingual gland, the mucous cells contained sulfated mucin, enzyme-resistant sialic acid and neutral mucin, and the serous demilunes contained neutral mucin and enzyme-resistant sialic acid.

• 보존과학회지
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• 제37권4호
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• pp.391-401
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• 2021

Outdoor sculptures of modern art works are being damaged and deteriorated as they are exposed to the outdoor environment due to the nature of exhibition in the outdoor environment, but secure of basic data through the measures for conservation and advanced researches still remain in the early stage. The surface of "Conversion" which is exhibited in the Busan Cheonmasan Sculpture Park has been exfoliated and deteriorated due to outdoor exhibition for a long time, so systematic conservation and management of works are considered necessary. Prior to the conservation and management, this study conducted observation of cross section, analysis of inorganic components, FT-IR, Raman and Py-GC/Mass analysis to examine the nature and type of paints used for the work through a scientific analysis. As a result of analysis, paints used for the "Conversion" include paint mixed with silvery aluminium powder and white pigment, reddish paint mixed with toluidine red, bluish paint that mixed prussian blue and titanium white and mixture of phthalocyanine blue and titanium white. The result is expected to be used as basic data for selecting materials necessary for conservative treatment of and establishing a plan for conservative treatment of the "Conversion".

• 대한치과교정학회지
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• 제6권1호
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• pp.33-38
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• 1976

For the purpose of evaluating the response of the periodontal tissue in relation to the experimental tooth movement, orthodontic force was applied to 15 female rats. The animals were investigated histopathologically and histochemically by several staining methods. Findings were as follows: 1. The application of 60gm on the rat's molar caused mesial tipping movement and undermining resorption. 2. One day after the insertion of the appliance, the osteoblast was increased in activity but not in number. 3. The osteoclastic activity was decreased as soon as removing the appliance. 4. With the P.A.S. staining, osteoclasts Stained mildly in the initial stage and stained more intensely in the last stage. Osteoclasts revealed negative reaction in the initial stage and stained mildly in the last stage. 5. Slides stained with toluidine blue revealed no conclusive variations between osteoblasts and osteoclasts.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.531-535
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• 1994

APUD cells in the oviduct of the sheep at standing estrus were localized as paraneurons in the lamina propria sandwiched between this structure and the tunica muscularis by the method of masked metachromasia to toluidine blue after hot mineral acid hydrolysis. These were also confirmed by lead haematoxylin stain and argyrophilia. The oviduct was serialized into 66 zones. Cells were absent in the first and last 2 zones, and most parts of the isthmus. There was however abundant number of APUD cells in the ampulla which were fusiform shaped and were about $5{\mu}m$ width and also in the juncture, where the cells were of a smaller width ($3{\mu}m$) and were quite numerous reaching 180-200 in some zones. It is concluded that peptide secreting cells are numerous in the oviduct and that this may qualify the oviduct as an endocrine organ.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2002년도 추계학술대회초록집
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• pp.136-136
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• 2002

A rat (Rottus norvegicus) infected with C. hepatica was trapped incidentally. At necropsy, grossly yellowish-white nodules were scattered on the liver surface. Microscopically, granulomatous and fibrotic nodules containing eggs and/or adult worms of C. hepatica were detected in the liver. Septal fibrosis forming pseudolobules was observed as a diffuse change throughout the liver. In double staining with immunostaining of -SMA and toluidine blue, myofibroblasts and mast cells were generally observed within the fibrous septa with mast cells being along the myofibroblasts. In this case, we hypothesized that myofibroblast and mast cell might playa role in septal fibrosis of rat liver induced by C. hepatica infection.

• Journal of Chest Surgery
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• 제23권3호
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• pp.537-541
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• 1990

Early esophageal carcinoma is defined as a lesion wherein invasion is confined to the mucosa and submucosa without metastasis to lymph node or other organs. Postoperative 5-year survival rate for early esophageal carcinoma is much superior than advanced carcinoma. Unfortunately, because of the anatomic characteristic of esophagus and absence of specific early symptoms, detection is frequently belated, and advanced disease is present at the time of the initial diagnosis. We experienced 2 cases of early esophageal carcinoma. They complained no specific symptoms. The diagnosis was made by barium esophagogram, esophagofiberscopy with dye staining and endoscopic biopsy. We performed esophagectomy with esophagogastrostomy. All had good postoperative course without any complication. We concluded that the combined use of double contrast radiography, esopagofiberscopy aided by intraluminal staining with Toluidine blue or Lugol`s solution, and endoscopic biopsy is very important in the diagnosis of early esophageal carcinoma in high risk patient group.

• 대한융합한의학회지
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• 제5권2호
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• pp.17-22
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• 2023

Objectives: This study aimed to investigate the anti-atopic properties of Glechoma hederacea var. longituba extracts (GHE) in murine atopic dermatitis model. Methods: BALB/c mouse ear stimulated with oxazolone (OX) for 4 weeks, then 1% GHE was topically applied every two days for 3 weeks to mouse. Ear thickness was measured by a digital thickness gauge. The ears tissues were collected and subjected to hematoxylin-eosin (H&E) and toluidine blue (TB) staining. Results: Treatment with GHE successfully alleviated the symptoms of atopic dermatitis, such as erythema, horny substance, and swelling. The infiltration of lymphocytes and mast cells were significantly reduced following GHE treatment. Conclusion: Taken together, our results showed that GHE possessed the potential to be a novel immunomodulatory drug against atopic dermatitis.

• 생명과학회지
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• 제14권3호
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• pp.396-399
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• 2004

본 실험은 고체배지를 이용한 새로운 실험방법을 이용하여 건조 및 염 스트레스에 대한 식물체의 형태학적인 특성을 뿌리신장률, 근단구조, 물질생산 등의 측면에서 조사하였다. PEG 및 NaCl 처리조건에서 처리 농도가 증가할수록 벼 식물체의 뿌리신장이 현저하게 저하되었으며, PEC 및 NaCl 처리에 따른 벼 식물체의 뿌리는 methyl-lignin 축적에 의한 리그닌화가 진행되었으며, 수분결핍을 극복하려는 기작으로 표피세포를 변형시킨 근모의 발생이 관찰되었다. 또한 PEG 및 NaCl 처리에 의한 벼 식물체의 지상부와 지하부의 건물 생산량의 현저한 감소는 지상부보다 지하부에서 뚜렷하였으며, 그 결과 PEG 및 NaCl 처 처리농도를 증가시킴에 따라 TR율의 증가를 보였다. 상기의 결과로부터 건조 및 염 처리를 한 고체배지를 이용함으로써 벼의 경우 스트레스 처리 전 약 2주간 유묘를 생육시키는 양액재배에 비해 발아기의 각종 스트레스에 대한 식물체 반응의 관찰과 내성 특성 검정에 유용할 것으로 사료된다.

• 한국임상수의학회지
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• 제31권2호
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• pp.95-101
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• 2014

Goat-anti-canine IgE polyclonal antibody로 유발한 개 피부염에 대한 0.1% FK-506 연고의 항염효과를 평가하였다. 임상적으로 건강한 성견 5두를 사용하여 실험군은 대조군, 위약적용군, FK-506 적용군으로 구분하였다. 항염효과는 팽진의 크기, 피부생검 검사를 통한 비만세포와 호산구수를 측정하여 평가하였으며 비만세포는 Toluidine blue, 호산구는 Luna 염색법을 사용하였다. 염증유발 15분 후의 팽진의 크기는 FK-506 적용군에서 유의성 있게 감소하였다 (p < 0.01). 6, 12, 24 시간 후 염증유발부위에 대한 피부생검을 각각 실시하였다. 정상피부의 비만세포는 $12.32{\pm}3.27cells/mm^2$ 이었으며 12시간 후에 FK-506 적용군은 $5.79{\pm}0.86cells/mm^2$으로 대조군의 $1.89{\pm}0.49cells/mm^2$, 위약군의 $1.74{\pm}0.50$ 에 비해 유의하게 증가하였다(p < 0.01). 정상피부의 호산구는 $2.73{\pm}2.24cells/mm^2$ 이었다. 대조군, 위약 적용군, FK-506 적용군의 호산구 수는 12 및 24 시간 후 FK-506 적용군에서 대조군과 위약 적용군에 비해 유의하게 증가하였다(p < 0.01). 따라서 FK-506 연고는 급성염증과 아토피증상과 유사한 후기염증에서 항염효과 있는 것으로 사료된다.