• Title/Summary/Keyword: tolerance analysis

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The Site-Directed A184S Mutation in the HTH Domain of the Global Regulator IrrE Enhances Deinococcus radiodurans R1 Tolerance to UV Radiation and MMC Shock

  • Zhang, Chen;Zhou, Zhengfu;Zhang, Wei;Chen, Zhen;Song, Yuan;Lu, Wei;Lin, Min;Chen, Ming
    • Journal of Microbiology and Biotechnology
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    • v.25 no.12
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    • pp.2125-2134
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    • 2015
  • IrrE is a highly conserved global regulator in the Deinococcus genus and contributes to survival from high doses of UV radiation, ionizing radiation, and desiccation. Drad-IrrE and Dgob-IrrE from Deinococcus radiodurans and Deinococcus gobiensis I-0 each share 66% sequence identity. However, Dgob-IrrE showed a stronger protection phenotype against UV radiation than Drad-IrrE in the D. radiodurans irrE-deletion mutant (ΔirrE), which may be due to amino acid residues differences around the DNA-binding HTH domain. Site-directed mutagenesis was used to generate a Drad-IrrE A184S single mutant, which has been characterized and compared with the ΔirrE mutant complemented strain with Drad-irrE, designated ΔirrE-E. The effects of the A184S mutation following UV radiation and mitomycin C (MMC) shock were determined. The A184S mutant displayed significantly increased resistance to UV radiation and MMC shock. The corresponding A184 site in Dgob-IrrE was inversely mutated, generating the S131A mutant, which exhibited a loss of resistance against UV radiation, MMC shock, and desiccation. qPCR analysis revealed that critical genes in the DNA repair system, such as recA, pprA, uvrA, and ddrB, were remarkably induced after UV radiation and MMC shock in the ΔirrE-IE and A184S mutants. These data suggested that A184S improves the ability against UV radiation and MMC shock, providing new insights into the modification of IrrE. We speculated that the serine residue may determine the efficiency of DNA binding, leading to the increased expression of IrrE-dependent genes important for protection against DNA damage.

OVI EMISSION LINE DETECTION LIMIT OF FAR-ULTAVIOLET IMAGING SPECTROGRAPH (과학위성 1호 탑재체 원자외선 분광기의 OVI 방출선 검출 한계)

  • 선광일;유광선;육인수;박장현;남욱원;한원용;선종호;민경옥
    • Journal of Astronomy and Space Sciences
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    • v.17 no.1
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    • pp.77-86
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    • 2000
  • There have been a few attempts to measure diffuse line emission between 900 $\AA$ and 1200 $\AA$, and only in a limited number of sight lines has it been detected. The main contributions to the equilibrium radiative cooling curve between $10^{4.5}K\;to\;10^6K$ are from the doublet of Ovi ${lambda}{lambda}1032;and;{lambda}{lambda}1038$ in the FUV spectral region. There are several bright airglow lines which could interfere with attempts to observe the OVI lines. The nearest lines HI 1025 $\AA$, OI 1027 $\AA$ have a combined intensity of about $10^{5.5}$ photons/s/$cm^2$/sr. In the present study, the detectability simulation of OVI doublet is performed using a Monte-Carlo technique and chi-square statistics. The analysis results are compared with the previous observations and with the predictions of several interstellar medium models, and are used to limit manufacturing and alignment errors of FIMS optical system.

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A Phenomenological Study on the Recovery Process of Elderly Women Living Alone in Suicide Crisis (자살위기를 벗어난 여성독거노인의 회복과정에 관한 현상학적 연구)

  • Lim, Jong-Rin;Kim, Joung-Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.8
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    • pp.304-314
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    • 2020
  • This study examined the experiences of the suicide crisis and recovery process by elderly women living alone to identify the essential meaning and components of life as a life to live again. In addition, the recovery of the research participants, as well as the phenomenon of such changes in thought and life, were examined. The results of this study were as follows. Data analysis yielded four components, eight sub-components, and 16 semantic unit reductions. There were four components of suicide attempts and recovery experiences of elderly women. They were "disconnection and disqualification," "repeated hardships," "road of prosperity in trouble," and "generous tolerance." The essential topics of these components have emerged not as individual but as holistic and contextually connectable topics. Finally, to summarize the results, the essential semantic structure of the suicide attempt and recovery experience of elderly women living alone is a result of a dysfunctional social structure and environment, which has placed pressure on the life of research participants. As an alternative method to overcome, they tried to solve the problem through the extreme practice of suicide attempts, but positive energy to live again due to emotional recovery factors recognized through suicide failures have made it possible to cope with the difficult reality.

Secure Jini Service Architecture Providing Ubiquitous Services Having Persistent States (유비쿼터스 서비스 상태지속을 지원하는 안전한 Jini 서비스 구조)

  • Kim, Sung-Ki;Jung, Jin-Chul;Park, Kyung-No;Min, Byoung-Joon
    • The KIPS Transactions:PartC
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    • v.15C no.3
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    • pp.157-166
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    • 2008
  • The ubiquitous service environment is poor in reliability of connection and also has a high probability that the intrusion against a system and the failure of the services may happen. Therefore, It is very important to guarantee that the legitimate users make use of trustable services from the viewpoint of security without discontinuance or obstacle of the services. In this paper, we point out the problems in the standard Jini service environment and analyze the Jgroup/ARM framework that has been developed in order to help fault tolerance of Jini services. In addition, we propose a secure Jini service architecture to satisfy the security, availability and quality of services on the basis of the analysis. The secure Jini service architecture we propose in this paper is able to protect a Jini system not only from faults such as network partition or server crash, but also from attacks exploiting flaws. It provides security mechanism for dynamic trust establishment among the service entities. Moreover, our secure Jini service architecture does not incur high computation costs to merge the user service states because of allocation of the replica based on each session of a user. Through the experiment on a test-bed, we have confirmed that proposed secure Jini service architecture is able to guarantee the persistence of the user service states at the level that the degradation of services quality is ignorable.

An analysis of determinants of purchase intension of individual pension using structural equation model (구조방정식모형을 이용한 개인연금 가입의향의 결정요인 분석)

  • Lee, Chanhee;Jung, Hongjoo
    • The Korean Journal of Applied Statistics
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    • v.29 no.3
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    • pp.409-424
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    • 2016
  • This study analyzed the causal relationship among factors that influence the purchase intensions for individual pensions which have a growing importance as a financial means after retirement. For this purpose, structural equation modeling based on the survey data (N = 928) was conducted to analyze psychological factors and financial factors comprehensively. According to empirical results of the structural equation, in the case of non-subscribers of individual pensions, potential longevity and coping efficacy influenced the purchase intensions for individual pensions fully mediated by fear and worry about longevity. Trust in insurers and risk tolerance directly influenced the purchase intensions for individual pensions. These show that trust in insurers is the most important factor in purchase intensions for individual pensions. Also, only trust in insurers give a direct impact on individual pensions in the case of individual pension subscribers. Longevity-related emotional side is expected to help in marketing activities that target potential customers signing individual pensions. In addition, trust in insurers has the greatest impact on new and additional subscriptions that represent critical efforts to improve trust in insurers.

KC-100 Full-scale Airframe Static Test (KC-100 전기체 정적 구조시험)

  • Shim, Jae-Yeul;Jung, Keunwan;Lee, Hanyong;Lee, Sang Keun;Hwang, Gui-Chul;Ahn, Seokmin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.42 no.1
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    • pp.67-75
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    • 2014
  • A full-scale static test for a composite structure small aircraft (KC-100) was conducted in the KARI. The test includes 15 full-scale test and 7 local test conditions. Test requirements with test schedule, test article with dummy structures, test load generation, test system, and equipment are introduced for the test. Test load data of the 1st test condition(U1) was analyzed to evaluate an accuracy of load control for the test. The analysis results show that load data obtained during test were within tolerance of Static Null Pacing Error(SNPE) and the error value of load control was 8.6N. The error of load controls for the full-scale static test using dozens of actuators was calculated by a method suggested by authors. Test data for all other test conditions is also shown in this paper. Finally, reactions measured from restraint system of the U1 test condition show that the reaction changes as load increment. The factors which may change the change of reactions for a full-scale static test are introduced in this study.

Non-point Source Critical Area Analysis and Embedded RUSLE Model Development for Soil Loss Management in the Congaree River Basin in South Carolina, USA

  • Rhee, Jin-Young;Im, Jung-Ho
    • Spatial Information Research
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    • v.14 no.4 s.39
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    • pp.363-377
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    • 2006
  • Mean annual soil loss was calculated and critical soil erosion areas were identified for the Congaree River Basin in South Carolina, USA using the Revised Universal Soil Loss Equation (RUSLE) model. In the RUSLE model, the mean annual soil loss (A) can be calculated by multiplying rainfall-runoff erosivity (R), soil erodibility (K), slope length and steepness (LS), crop-management (C), and support practice (P) factors. The critical soil erosion areas can be identified as the areas with soil loss amounts (A) greater than the soil loss tolerance (T) factor More than 10% of the total area was identified as a critical soil erosion area. Among seven subwatersheds within the Congaree River Basin, the urban areas of the Congaree Creek and the Gills Creek subwatersheds as well as the agricultural area of the Cedar Creek subwatershed appeared to be exposed to the risk of severe soil loss. As a prototype model for examining future effect of human and/or nature-induced changes on soil erosion, the RUSLE model customized for the area was embedded into ESRI ArcGIS ArcMap 9.0 using Visual Basic for Applications. Using the embedded model, users can modify C, LS, and P-factor values for each subwatershed by changing conditions such as land cover, canopy type, ground cover type, slope, type of agriculture, and agricultural practice types. The result mean annual soil loss and critical soil erosion areas can be compared to the ones with existing conditions and used for further soil loss management for the area.

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Preliminary Design of the NISS onboard NEXTSat-1

  • Jeong, Woong-Seob;Park, Sung-Joon;Moon, Bongkon;Lee, Dae-Hee;Park, Won-Kee;Lee, Duk-Hang;Ko, Kyeongyeon;Pyo, Jeonghyun;Kim, Il-Joong;Park, Youngsik;Nam, Ukwon;Park, Chan;Im, Myungshin;Lee, Hyung Mok;Lee, Jeong-Eun;Shin, Goo-Hwan;Chae, Jangsoo;Matsumoto, Toshio
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.2
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    • pp.102-102
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    • 2014
  • The NISS (Near-infrared Imaging Spectrometer for Star formation history) onboard NEXTSat-1 is the near-infrared instrument onboard NEXTSat-1 which is being developed by KASI. The main scientific targets are nearby galaxies, galaxy clusters, star-forming regions and low background regions in order to study the cosmic star formation history in local and distant universe. After the Preliminary Design Review, we have fixed major specifications of the NISS. The off-axis optical design with 15cm apertureis optimized to obtain a wide field of view ($2deg.{\times}2deg.$), while minimizing the sensitivity loss. The opto-mechanical structure of the NISS was designed to be safe enough to endure in the launching condition as well as the space environment. The tolerance analysis was performed to cover the wide wavelength range from 0.95 to $3.8{\mu}m$ and to reduce the degradation of optical performance due to thermal variation at the target temperature, 200K. The $1k{\times}1k$ infrared sensor is operated in the dewar at 80K stage. We confirmed that the NISS can be cooled down to below 200K in the nominal orbit through a radiative cooling. Here, we report the preliminary design of the NISS.

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An Analysis on the Properties of Beam Coupling by Using Gaussian Beam Propagation Theory (가우시언 빔 전송 이론을 이용한 빔 결합 특성 해석)

  • Han, Seog-Tae;Kang, Jin-Man;Lee, Jeong-Won;Je, Do-Hyung;Jung, Moon-Hee;Kim, Soo-Yeon;Wi, Seog-Oh
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.11
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    • pp.1324-1333
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    • 2010
  • In this paper, three kinds of beam coupling losses which occur in a quasi-optics circuit for millimeter wave receiver system have been intensively investigated. First, the beam coupling losses which are caused by mismatch of beam waists radii and their positions between those of one and the other have been evaluated. It shows that beam coupling losses due to mismatch of beam waists radii and their positions between two quasi-optics circuits can be minimized if beam waist radius is chosen as larger than 3 times the operation wavelength. Second, the beam coupling losses have been studied when the axis of propagation of one beam is tilted with respect to that of the other beam. It is noted that smaller beam waist radius results in greater tolerance to tilts and angular misalignments. Third, the beam coupling cases in which two beams are offset if their axes of propagation are parallel but one is displaced relative to the other have been investigated. It is confirmed that beam waists radii with larger than 3 times operation wavelength are less sensitive to lateral offsets.

The Effects of Body Mass Index on Baseline Hormonal Status and Glucose Metabolism in Women with Chronic Anovulation (비만 지표 (Body Mass Index)가 만성 무배란 여성의 혈중 기저 호르몬치와 포도당 대사에 미치는 영향)

  • Rhee, Jeong-Ho;Jeong, Eun-Jeong;Kim, Jong-In
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.1
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    • pp.67-76
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    • 2002
  • Objective: To assess the difference of baseline hormonal status and pathophysio logy, and confirm the risk factors for long term complication according to Body Mass Index in women with polycystic ovary syndrome. Materials and Methods: Serum level of LH, FSH, Estradiol, Prolactin, Testosterone, DHEA-S, fasting insulin were measured and 100 gm oral glucose tolerance test and endometrial biopsy were performed in total 75 chronic anovulation patients and 20 normal cycling infertility patients. 95 evaluated patients were divided into 3 groups including patients with chronic anovulation having BMI below 25, BMI beyond 25.1, normal cycling infertility patients, Group 1 (n=39), Group 2 (n=36), Group 3 (n=20), respectively. Statistical analysis was performed respect to relationship between BMI and measured hormone level, sum of glucose level during 100 gm OGTT, insulin resistance using t-test, ANOVA test, Post Hoc test, Mann-Whitney test. p<0.05 was considered as statistically significant. Results: Serum LH level and LH/FSH ratio was significantly higher in Group 1, compared than Group 2 or 3 (p<0.05), BMI and LH, LH/FSH ratio was negatively correlated (r=-0.351, r=-0.318). There was no significant difference according to BMI in FSH, testosterone, estradiol, prolactin, DHEA-S level. Fasting insulin and sum of glucose level during 100 gm OGTT were significantly higher in Group 2 compared than Group 1 or Group 3 (p<0.05), there was no significant difference between Group 1 and Group 3. Insulin resistance was more frequently identified in Group 2 compared than Group 1 (p=0.001). Conclusions: BMI and LH, LH/FSH ratio were negatively correlated, so clinical significance of LH, LH/FSH ratio in diagnosis of PCOS may be attenuated by increasing body weight. Overweight patients with chronic anovulation may be the risk group for developing insulin resistance, hyperinsulinemia, glucose intolerance, later type 2 DM. Hyperinsulinemia may operate mainly in overweight chronic anovulation patients in development of hyperandrogenism.