• The Korean Journal of Mycology
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• v.28 no.1
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• pp.41-45
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• 2000

The histological studies were conducted to investigate the symbiotic relationships between Gastrodia elata and Armillaria mellea by using light and electron microscopes. The fungus, A. mellea, penetrated into the cortex of G. elata, in which endomycorrhizal mycelia in the cortical cells appeared to be dissolved and digested, and seemed to be consequently used as nutritional sources for G. elata growth. Staining of infected tissues revealed that protein- and fat-like substances were localized in the cells. The nuclei of cells infected by the fungal mycelia were hypertrophied 1.5 to 2 times as those without the fungal infection.

• Korean journal of applied entomology
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• v.27 no.4
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• pp.219-224
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• 1988

Rearing of Spodoptera litura fed on arfificial diet basically formulated with kindby bean powder was much promisible for production of S. litura nuclear polyhedrosis virus(SINPV) than that on kidney bean leaves. The $LC_{50}$ of SINPV against 3rd and 5th instar were $4.48{\times}10^{3}$ PIB/ml and $4.52{\times}10^{4}$ PIB/ml, respectively. The $LC_{50}${/TEX} of SINPV varied from 5.8 days to 7.7 days at higher inocula in the testes. The larval growth of 1st and 3rd instar larvae fed on virus were much delayed and the larval were killed within 4th instar in the former and within 5th instar in latter. S. litura larvae were very susceptible to multinucleocapsid nuclear polyhedsosis virus (MNPV) of Autographa californica, S. littoralis and Trichoplusia ni.

• Korean Journal of Medicinal Crop Science
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• v.1 no.2
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• pp.158-161
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• 1993

This experiments were carried out determine planting date for highest Yields and select an covering material for enhancement of environments in Cassia tora L.The highest Yields by regressions estimate produced 352 kg/10a at May 18. of coure, it is possible to plant at June and early-July in spite of slight decreas of Yields, therefore Cassia tora L. is benefit to establish cropping system with barley, wheat or other crops. The covering materials for highest yielding was Black-white nonporous PE(low density) and it out yielded about 53% than conventional cultivations. Cassia tora L. is possible to produce Without agricultural medicines because of little occurence of decrease except occuring of Damping off(Rhizoctonia solanikuhn) at early planting.

• Korean Journal of Environmental Agriculture
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• v.21 no.1
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• pp.24-30
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• 2002

Ginseng (Panax ginseng C. A. Meyer) has been cultivated on raised beds with shading condition for 4 to 6 years because of its physiological characteristics. This shading condition maintains relatively stable soil environments protecting from rainfall and direct sunlight. Therefore, pesticides in ginseng growing soil are exposed to far different environment from the general cropping field. To study the behavior of pesticides under this condition, the effect of covering on the persistency of Procymidone in ginseng cultivating soil was investigated by using several shade materials. The most important factor affecting Procymidone persistency in soil under covering was water leakage rates of shade materials.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.15-18
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• 2002

Callus and shoot formation of leaf explants from in vitro propagated shoots and field grown plants depending on the position of leaf, and four boxthron cultivars were investigated. Callus formation of explants from both in vitro shoot and field grown plants as easily achieved at the cut surfaces of explants but the callus formed from leaf of in vitro shoots was hardened as the duration of culture was proceed. Calli were effectively induced from leaves detached from the middle position of both in vitro and in vitro plants on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA, and the growth of calli were better in field grown leaves than in vitro grown leaves. Shoot formation were effectively induced from leaves detached from the upper position in vitro plants, and the middle parts of in vitro plants on MS medium containing 0.01 mg/L (NAA with 0.2 mg/ BA. There was difference on the frequency of shoot formation among four different cultivars; 'Jindojaerae' was the best for shoot formation followed by 'Cheonyang', 'Younghagukija' and 'Cheongyangjaerae'.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.87-93
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• 1982

Dihaploid lines derived from the another of the $F_1$ of single cross, SC72 $\times$ Hicks (Nicotiana tabacum L.) were estimated the variation, heritablities, genetic correlations and path-co-efficients on agronomic characters and chemical compositions. Dinaploids exhibited reduced vigor and growth when comparecd with the mid-parent value but had increased nicotine contents, and the range of dihaploids characters were deviated more than that of their parents. Heritabilities of days to flowering, leaves per plant, nicotine. contents, leaf size and plant height appeared high value, and the yield, value per kg. and reducing sugar contents were low. There were positive genetic correlations between yield, and value per kg., leaf width, leaves per plant and reducing sugar contents and negative genetic correlations appeared between nicotine contents and the other characters except for index of leaf shape. Path-coefficient analysis of genotypic correlation coefficients showed that leaves per plant and leaf width lave a high direct effect, and plant height and days to flowering have a high indirect effect on yield, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.366-375
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• 1984

In order to confirm the effect of climatic consumption index (C C I) on the number of spikelets and yield of rice, 3 levels of shading rates such as 0, 25, 50% of full light were treated during the tillering stage, reproductive growth stage and ripening period, respectively, in a phytotron controlled with day/ night temperature of 20/10$^{\circ}C$ and 30/20$^{\circ}C$, and field at Crop Experiment Station, Suwon, Korea. The results are as follows: 1. As solar radiation decreased during the reproductive growth stage in 30/20$^{\circ}C$ or field condition, the number of spikelets per panicle was decreased due to the decrease of the number of differentiated secondary rachis branches and spikelets as well as the increase of the number of degenerated secondary rachis branches and spikelets. 2. Our results showed slight negative correlation between C C I of the reproductive growth stage and number of panicles per square meter and number of differentiated secondary rachis branches. On the other hand, there was highly significant positive correlation between C C I of the reproductive growth stage and the number of degenerated secondary rachis branches and spikelets, and negative correlation in number of differentiated spikelets. 3. The shading during the reproductive growth stage did not affect on the percentage of ripened grains and 1000 grains weight of hulled rice, whereas those were decreased with shading during the ripened period. 4. Influence of shading in each growing stage on the yield was severe in the order of ripened period, reproductive growth stage, tillering stage. 5. Respiration rate in Jinheung was higher than that of Tongil at low temperature, but reversed above 30$^{\circ}C$. Respiratory coefficients (Q$\sub$10/) of Tongil and Jinheung were 2.74 and 1.96, respectively. Respiration/ photosynthesis ratio in Jinheung was higher than that of Tongil at low temperature, while higher in Tongil above 32$^{\circ}C$. 6. Transportation of $\^$14/C was restricted at 20/10$^{\circ}C$ in Tongil, however, there was no differences at 30/20$^{\circ}C$ in both Tongil and Jinheung. The influence of shading on the transportation of $\^$14/C did not affect at 20/10$^{\circ}C$, but it was hampered with shading at 30/20$^{\circ}C$ in both varieties.

• Journal of Ginseng Research
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• v.23 no.2 s.54
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• pp.93-98
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• 1999

In order to evalulate the qualities of red ginseng extract and decrease precipitate fonnation in ginseng drink, red ginseng extract was hydrolized with ${\beta}-amylase$ and transglucosidase. $5.2\%$ isomaltose was produced as isomaltooligosaccharides and glucose content was increased in the enzyme treated ginseng extract. Contents of ginsenoside $R-b_1\;and\;R-b_2$ were decreased, whereas ginsenoside-Rd was increased by the enzyme treatments. The growth of 3 strains of bifidus spp. and 4 strains of lactobacillus spp., beneficial intestinal bacteria, were enhanced by adding of the enzymatically hydrolized ginseng extract. Sweetness and sourness were increased, however, bitterness and astringency were decreased in the hydrolized ginseng extract. The fonnation of precipitates in hydrolized red ginseng extract of $pH\;3.0\~4.5$ were significantly decreased in the storage condition of $40^{\circ}C$ for 1 month compared to that of control.

• Korean Journal of Organic Agriculture
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• v.25 no.4
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• pp.737-747
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• 2017

This experiment was carried out to determine the optimal concentration of agricultural organic materials using sulfur and oil for the insect pest control in pepper and cherry tomato cultivation. The control value of aphids and Oriental tobacco budworm (OTB) was examined one day after spraying with sulfur preparation (SP) (0.33~0.17%), oil preparations (OP) (2.00~0.33%), SP+OP, OP+ginkgo leaf extracts (GLE), SP+OP+GLE on the "Super Manidaa"pepper. The aphid control in pepper was complete by applications of SP+OP (0.25+1.00%) in the early growth stage and the control value was above 98.1% by the application of OP+GLE (1.00+1.00 %), SP+OP+GLE (0.25+1.0+1%), SP+OP+GLE (0.25+1.0+0.5%) in the middle to late growth stage while showing 0% in the control treatment. The OTB was completely controlled by the 3 times application with the high concentration of SP+OP (0.25+1.00%) in pepper cultivation. This result indicates that the oil and the sulfur preparations should be applied at low concentration before insect pests do not appeared, and then sprayed at the high concentration after they appear at pepper plant. The greenhouse whitefly in 'Minichal' tomatoes was completely controlled by three times application of SP (0.25~0.33), OP (1.0~2.00%). and all the treatment of SP+OP. However, continuous control with intervals of 1~3 days was considered favorable in the tomato plant. By the periodical control with agricultural organic materials using sulfur and oil, the greenhouse whitefly, which is a high-temperature insect pest, several moths of OTB did not occur at all. In conclusion, SP+OP (0.17%+0.33%) treatment was the most economical combination to control the aphid, OTB, and greenhouse whitefly in pepper and tomato cultivation when considering operating cost. In addition, we recommend that SP should not be sprayed on the plant shoots during the day time from July to August because of high temperature.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.235-241
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• 2012

Sumoylation is a reversible conjugation process that attaches the small ubiquitin modifier (SUMO) peptide to target proteins and regulates a wide variety of cellular functions in eucaryotes. As final step of the sumoylation, SUMO E3 ligases facilitate conjugation of SUMO to target proteins. To characterize the functions of the SUMO E3 ligases in Oryza sativa, we isolated a single recessive rice SUMO E3 ligase, Ossiz1-2 mutant. In addition, we also confirmed the interaction between OsSIZ1/-2 and OsSUMO1, respectively, by using an Agrobacterium-based tobacco luciferase transient expression system. Ossiz1-2 mutant exhibited approximately 20% reduction in growth and developmental units compared with wild type. Especially, number of filled seeds and total seed weight were dramatically decreased in the Ossiz1-2 mutant rice. Thus, these results suggest that sumoylation by the OsSIZ1 as SUMO E3 ligase plays an important role in regulating growth and development in rice.