• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.359-366
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• 2005

Effects of neutron beam irradiation on seed germination, growth and RAPD pattern of tobacco (Nicotiana tabacum L. cv.; N. plumbaginifolia) and rice (Orya sativa L. cv.) plants were estimated. Seed germination rate was not significantly changed by the neutron beam treatment in both tobacco and rice seeds. And there was no significant differance in growth of the plants by the neutron beam treatment. Interestingly, however, some of morphological changes, including leaf shape (about $36\%$), stem color and leaf color were observed in neutron beam treated tobacco plants. In addition, abnormal flower in petal was observed in the neutron beam treated plant. This results indicate that neutron beam is able to use as an effective mutagen in plant mutations. Scorable products from 20 primers were obtained by RAPD analysis in the leaves of the beam irradiated tobacco plants and most of the plants showed the similar band patterns.

• Journal of the Korean Society of Tobacco Science
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• v.11 no.1
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• pp.49-64
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• 1989

The study was carried out to investigate the effect of control leaf number on growth and chemical characteristics, and on yield and quality In (rue-cured tobacco The rest upper leaves of 14, 16, and 18 leaves per plant were removed at button stage, and the plants were harvested :0, 12, and 16 leaves with discard 4 leaves of the bottom, respectively. 1. There were showed a tendency to increase in length (LL), width (LW), area (LA), fresh (LFW) and dry weight (LDW) per leaf as leaf numbers were decreased, while those per plant and the ratios of increase of those per plant were decreased. 2. In compensation effect (CE), there were increase in LL, LFW, LDW, LL/LW and LDW/LFW with each decreased in leaf numbers while LW was decreased, LA unchanged. 3. There were significant Increases in LL, LW, and LA at 5th~8th stalk positions form bottom (SP), while those .at 13th~16th SF were decreased. Also, LDW at 5th~l0th, and LDW/LFW and weight Per unit leaf area at 11th 16th were Increased as leaf numbers decreased. 4. Mean per plant and CE of nicotine were increased, but those of sugar were decreased with each decrease In leaf numbers. In addition, nicotine contents at 5th~14th SP except for 9th~10th were increased, otherwise sugar contents at 7th~14th were decreased. 5. There were showed a tendency to increase in grade per Plant and in tirade at 5th~8th SP, but decrease in CE of grade as leaf numbers were decreased. There wee significant decrease in grade at 11th~14th SP. Also, ratio of 5 grade was decreased, while ratios of 3 and 4 grades were increased. 6. When the 2 leaves at 3rd~4th SP were harvested, price was decrease 2.6% and yield was increased 5.8 %. 7. When the 6 leaves from convention were removed at but ton stage, effects of apparent, compensational, and net per leaf on yield were -3.9%, +2.6%, and -6.5%, respectively, and those on price were +1.1%, -1.6% and +2.7%, respectively.

• Korean Journal of Plant Resources
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• v.12 no.3
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• pp.171-178
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• 1999

The purpose of this study was to investigate the optimal mixture ratio for the mycelial culture of the Pleurotus ostreatus. The chief cultural media in this study were cotton hull, sawdust and rice straw and the supplemental media were zeolite, corn cob, defatted rice bran, white cotton, tobacco trash powder, rice hull and peat. The results of this study were as follows; the optimal mixture ratio of the chief cultural media were effective in 6 : 3 : 1(V/V, %), and the mycelial growth and density in the supplemental media were considerably better 1% zeolite,3% corn cob, 5% defatted rice bran, 1% white cotton, 1% tobacco trash powder, 7% rice hull in good order. The optimal mixture ratio be to the mixed supplemental media in the chief cultural media were as follows ; 2 : 2(V/V, %) at the conditions of mixed zeolite and corn cob; 3 : 2(V/V, %) at the conditions of mixed defatted rice bran and white cotton; 1 : 3(V/V, %) at the conditions of mixed tobacco trash powder and rice hull. At the conditions of the whole cultural media mixed, the mycelial growth and density were in good conditions ; cotton hull, sawdust, rice straw, zeolite, corn cob, defatted rice bran, white cotton, tobacco trash powder, rice hull, and peat were mixed 43.0 : 17.2 : 25.8 : 2.0 : 2.0 : 3.0 : 2.0 : 1.0 : 3.0 : 1.0 (V/V, %).

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.73-73
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• 2005

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.175-189
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• 1990

Nitrogen compounds of Panax ginseng and their biological activities in plant and animal were reviewed. Major nitrogen compounds found in P. ginseng are free amino acids. Water solilble proteins, indouble proteins and peptides. Minor nitrogen compounds are dencichine. Glycolyroteins, amines, alkaloides, methoxy or alkyl pyrazine derivatives, free nucleosides and nucleic acid bases. 4-methyl-i-thiazoltethanol and pyroglutamic acid the contents of total nitrogen and protein in root Increased until 13 years old which was the highest age tinder investigation. Soluble protein content increased with the root weight and was higher in xylem pith than cortex-epidermis indicating the close relation with root growth. Arginine, which covered 58% of total free amino acids, may serve as storage nitrogen. Arginine seems to be changed into proline in rhizome. threonine in stem and again threonine and arginine in leaf. The greater the root weight the higher the polyamine stimulated Polyamine stimlllated the growth of root callus. Physiological roles of other minor nitrogen compounds are unknown although content is relatively high ((1.if) 6.w). Biochemical and pharmacological activities of some nitrogen compounds for animal were more investigated than physiological role there plant itself. Radiation and U.V protective function (heat stable protein). insulin-like activity in lipogenesis and livolysis (adenosine and pyroglutamic acid), depression of blood sugar content (glycopevtide). htmostatic and nellrotoxic activity (dencichine) and, sedative and hypnotic activity (4-methyl-i-thiazoleethanol) are reported. Heat stable protein increased with root age. The traditional quality criteria appear to be well in accordance with biological activities of nitrogen compounds. Chemical studies of nitrogen compounds seem relatively rare, probably due to difficulty of isolation, subsequently the investigations of biological activities are little.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.143-146
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• 1989

In order to clarify the characteristics of anthocyanln-producing cell line selected by in vitro grown cultures of ginseng (Panax ginseng C.A. Meyer), the calli of anthocyanin-Producing strain were cultured on media supplemented with different concentrations of 2,4-D and sucrose under light or dark condition. The light was found to be essential for anthocyanin synthesis. Anthocyanin synthesis of ginseng cell line was inhibited by the increase of 2,4-D in the medium. On the other hand, sucrose promoted the anthocyanin-production and the optimum concentration of sucrose for the highest production of anthocyanin was 5%. The growth of anthocyanin-producing tell line was best on the medium supplemented with 0.5 mg/l 2,4-D and 5% sucrose.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.1
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• pp.32-37
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• 1985

Effects of straw mulching on growth of shoot and root of Panax ginseng, soil physical and chemical characteristics were investigated. Straw mulching increased root yield, stem length, leaf area and decreased defoliated plant rate and missing root rate. Percent solid phase and soil hardness decreased, and porosity, percent air phase and percent liquid phase increased under straw mulch. It increased soil water content and decreased soil temperature especially in the rear side of the bed. Available soil phosphorus (very harmfully in some case), exchangeable potassium, organic matter increased while exchangeable calcium decreased and the lowering of pH was retarded under mulch. Straw mulching greatly reduced need population, prevented soil covering on the lower side of leaves and eliminated solings to plants, bed sides and on bed top.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.692-697
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• 1996

This experiment was conducted to determine the influences of growth stage, stalk position and growth environment on duvatrienediols(DVT-diols) and cis-abienol production in Nicotiana tabacum L. The leaves of plants at transplanting stage contained very small amount of duvatrienediols and cis-abienol. Comparing leaves on different stalk position at flowering stage, upper and middle leaves contained more DVT-diols and cis-abienol than lower leaves. Plants grown under controlled environmental room at 3$0^{\circ}C$ contained more DVT-diols and cis-abienols than room at 18$^{\circ}C$, $25^{\circ}C$ and glass room. Tobacco plant grown under field condition produced lower levels of duvatrienediols and cis-abienol contents than glass room-grown plants. The amounts of duvatrienediols and cis-abienol of tobacco leaves significantly decreased after rain under field conditions.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.317-331
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• 1990

Nitrogen compounds of Panax ginseng and their biological activities in plant and animal were reviewed. Major nitrogen combounds found in P. ginseng are free amino acids, Water soluble teins, insoluble proteins and peptides. Minor nitrogen compounds are dencichine. glycol)roteins. amines, alkaloides, methoxy or alkyl pyrazine derivatives. free nucleosides and nllrleir arid bases. 4-me- thymi-5-thiazoleethanol and pyroglutamic acid. The contents of total nitrogen and protein in root increased until 13 years old rvhich was the highest age tinder investigation. Soluble protein content increased With the root weight and was higher in xylem pith than cortex-epidermis indicating the rlosc relation with root growth. Arginine which covered 58% of total free amino aroids may serve as a storage nitrogen. Arginine seems to be changed into proline in rhizome, threonine in stem and again threoning and arginine in leaf. The greater the root weight the higher the polyaminc content. Polyamine stimulated the growth of root callus. Physiological roles of other minor nitrogen compounds are unknown although dencichine content is relatively high (0.5% d.w.). biochemical and pharmatological activities of some nitrogen compounds for animal were more investigated than physiological roll iota plant itself. Radiation and U.V. protective function (heat stable protein), insulin-like activity in lipogenesis and lipolysis (adenosine and pyroglutamic acid), depression of blood sugar content (glycopeptide). hemostatir and nellrotoxic activity (denrichine) and. sedative and hypnotic activity (4-methyl-5-thiazoleethilnol) are reported. Heat stable protein increased with root age. The traditional quality critsria appear to be well in accordance with biological activities of nitrogen compounds. Chemical stlldies of nitrogen compounds seem relatively rare, probably dole to difficulty of isolation, subsequently the investigations of biological activities are little.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.215-220
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• 1989

For development of slow releaseing K-fertilizer using natural zeolite, two kinds of K adsorbed zeolite were made by ion exchange reaction in $K^+$ saturated aqueous solution. K-Zeolite was prepared by removing the excess salt after K adsorption on zeolite, however, NW. K-Zeolite was prepared only by K adsorption reaction without removing the excess salt. The adsorbed K on zeolite was not easily released, but continuously and slowly released from the surface of zeolite. By the results of application for tobacco cultivation, the K contents in soil treated with K-Zeolite and NW. K-Zeolite were lower than that of control (20kg/10a) at the early growth stage, but were higher at the late growth stage. Therefore, the results suggest that K adsorbed zeolite could be applied as a slow-releasing K fertilizer.