• Archives of Reconstructive Microsurgery
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• 제23권2호
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• pp.70-75
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• 2014

Purpose: Soft-tissue reconstruction in the knee area requires thin, pliable, and tough skin. The range of motion of the knee also acts as a limitation in using only local flaps for coverage. The author has successfully used various perforator flaps for soft tissue reconstruction around the knee while preserving its functional and cosmetic characteristics. Materials and Methods: Out of the twenty patients assessed from April 2009 to March 2011, seven received anterolateral thigh perforator flaps, four received medial sural perforator island flaps, four received lateral supragenicular perforaor perforator flaps, and five received medial genicular artery flaps. The age of the patients ranged from 44 to 79 and the size of the defects ranged from $4{\times}5cm$ to $17{\times}11cm$. Fifteen of the twenty patients had histories of total knee replacement (TKR) surgery. Results: There were no flap losses in any of the twenty patients assessed. Two patients showed partial losses in the distal area of the flap, but were treated through careful wound care. One patient presented with pedicle adhesion at the drainage site from a past TKR, but it did not hinder the flap survival. Primary closure at the donor site was possible in nine patients, while split skin graft was necessary for the other 13. Conclusion: In soft tissue reconstruction of the knee, various perforator flaps can be used depending on the condition of the preoperation scar, wound site, and size. It also proved to provide better functional and cosmetic results than in primary wound closure or skin grafts.

• The Korean Journal of Physiology and Pharmacology
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• 제15권2호
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• pp.83-88
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• 2011

A large body of evidence has indicated that induction of endogenous antioxidative proteins seems to be a reasonable strategy for delaying the progression of cell injury. In our previous study, cilostazol was found to increase the expression of the antioxidant enzyme heme oxygenase-1 (HO-1) in synovial cells. Thus, the present study was undertaken to examine whether cilostazol is able to counteract tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced cell death in endothelial cells via the induction of HO-1 expression. We exposed human umbilical vein endothelial cells (HUVECs) to TNF-${\alpha}$ (50 ng/ml), with or without cilostazol ($10{\mu}M$). Pretreatment with cilostazol markedly reduced TNF-${\alpha}$-induced viability loss in the HUVECs, which was reversed by zinc protoporphyrine IX (ZnPP), an inhibitor of HO-1. Moreover, cilostazol increased HO-1 protein and mRNA expression. Cilostazol-induced HO-1 induction was markedly attenuated not only by ZnPP but also by copper-protoporphyrin IX (CuPP). In an assay measuring peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$) transcription activity, cilostazol directly increased PPAR-${\gamma}$ transcriptional activity which was completely abolished by HO-1 inhibitor. Furthermore, increased PPAR-${\gamma}$ activity by cilostazol and rosiglitazone was completely abolished in cells transfected with HO-1 siRNA. Taken together, these results indicate that cilostazol up-regulates HO-1 and protects cells against TNF-${\alpha}$-induced endothelial cytotoxicity via a PPAR-${\gamma}$-dependent pathway.

• Applied Biological Chemistry
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• 제38권5호
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• pp.414-421
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• 1995

Lipoxygenase (LOX) 효소의 최적 활성 조건과 활성 억제등 LOX 활성 측정의 중요한 정보를 확립하기 위하여, 추출 Buffer의 영향, 기질, pH, 저장, 온도, NaCl, $CaCl_2$외 cations 및 antioxidants의 요소들이 LOX 활성에 미치는 영향을 조사하였다. 그리고 오이 tissue내의 LOX의 편재도 시험하였다. LOX에 대한 우수한 기질은 linolenic acid, linoleic acid, arachidonic acid 순서였다. 오이 껍질이나, mesocarp tissue내에 존재하는 LOX의 활성은 pH 5.5가 최적 조건이었으며, 섭씨 $40^{\circ}C$와 $50^{\circ}C$에서는 비교적 안정성을 보였다. LOX의 활성은 pH 5.0와 0.2M NaCl 조건을 같이 주었을때 opitimum 안정성을 보였다. LOX 활성은 $Mn^{2+},\;Cu^{2+}$ 또는 $Al^{3+}$와 같은 양이온에 의해서는 감소되었지만, 오히려 $Ca^{2+}$은 효소의 활성을 자극시켰다. 한편 butylated hydroxy anisole (BHA)와 propyl gallate의 농도가 증가할수록 LOX 활성은 감소되었다. 오이 껍질에서의 LOX의 활성은 다른 tissue에, locule, mesocarp, 비해 최고치를 보였다.

• 대한족부족관절학회지
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• 제6권2호
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• pp.144-148
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• 2002

Purpose: To evaluate clinical characteristics of soft-tissue masses around the foot Materials and Methods: Sixty seven cases of soft tissue masses around the foot, excised at Kang Dong Sacred Heart hospital from Jan. 1987 to Oct. 2000, were included in the study. The age and sex of the patient, location and size of the lesion, history of trauma, presence or absence of pain and neurological symptoms as wellas final pathological diagnosis were obtained from retrospective analysis. Results: For type of lesion, all lesions were benign. Ganglion was 23 cases, epidermal inclusion cyst was 10 cases, lipoma was 8 cases, hemangioma was 5 cases and fibroma was 4 cases. For age, 63 percent of the patients were either between the ages of twenty and thirty nine or between the ages of fifty and fifty nine. For sex, the male-to-female ratio was 1 to 1.4. For zones of the foot, 18 cases were in Zone 1, 17 in Zone 4, 12 in Zone 2, 12 in Zone 3 and 8 in Zone 5. For clinical finding, 18 cases had pain. Conclusion: Ganglion was the most common lesions, followed in order of frequency by epidermal inclusion cyst, lipoma, hemangioma and fibroma. Lesions occurred frequently at Edward and Michael Zone 1, 4 and pain was the most common symptom.

• Applied Biological Chemistry
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• 제39권3호
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• pp.222-226
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• 1996

제조과정, 과산화물 및 저장기간에 따른 냉장 Dill 피클과 brine에 함유된 lipoxygenase, peroxidase, catalase 효소의 활성 변화를 조사하였다. 오이를 Dill 피클용 brine에 1일 저장시의 lipoxygenase는 거의 비활성화 되었으나, 1일 이후에는 lipoxygenase의 활성은 서서히 감소하였다. 반면, 오이 tissue에 존재하는 peroxidase의 활성은 피클 제조후 4일 저장기간 동안 서서히 감소 현상을 나타내었다. 신선한 오이 tissue에서 추출된 catalase는 높은 활성을 가지나, 오이를 피클용 brine에 담갔을 경우에는 소량의 catalase 활성만 보였다. Brine에 과산화물을 첨가시, tissue에서 추출된 lipoxygenase, peroxidase, catalase 활성은 저장 기간과 과산화물 농도에 따라 지속적인 감소를 보였다.

• Archives of Plastic Surgery
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• 제49권1호
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• pp.61-69
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• 2022

Background Single free flaps are a commonly used reconstructive method for multiple soft tissue defects in digits. We analyzed the flap size, division timing, and degree of necrosis in cases with various types of flap division. Methods We conducted a retrospective review of the medical charts of patients who had undergone single free flap reconstruction for multiple soft tissue defects across their digits from 2011 to 2020. The flap types included were the lateral arm free flap, venous forearm free flap, thenar free flap, hypothenar free flap, anterolateral thigh free flap, medial plantar free flap, and second toe pulp free flap. Flap size, anastomosed vessels, division timing, and occurrence of flap necrosis were retrospectively investigated and then analyzed using the t-test. Results In total, 75 patients were included in the analysis. The success rate of the free flaps was 97.3%. All flaps were successfully divided after at least 17 days, with a mean of 47.17 days (range, 17-243 days) for large flaps and 42.81 days (range, 20-130 days) for the medium and small flaps (P=0.596). The mean area of flap necrosis was 2.38% in the large flaps and 2.58% in the medium and small flaps (P=0.935). Severe necrosis of the divided flap developed in two patients who had undergone flap division at week 6 and week 34. Conclusions In cases where blood flow to the flap has been stable for more than 3 weeks, flap division can be safely attempted regardless of the flap size.

• Molecules and Cells
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• 제43권10호
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• pp.856-869
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• 2020

To elucidate the mechanism of action of HOXA11-AS in modulating the cisplatin resistance of nasopharyngeal carcinoma (NPC) cells. HOXA11-AS and miR-454-3p expression in NPC tissue and cisplatin-resistant NPC cells were measured via quantitative reverse transcriptase polymerase chain reaction. NPC parental cells (C666-1 and HNE1) and cisplatin-resistant cells (C666-1/DDP and HNE1/DDP) were transfected and divided into different groups, after which the MTT method was used to determine the inhibitory concentration 50 (IC₅₀) of cells treated with different concentrations of cisplatin. Additionally, a clone formation assay, flow cytometry and Western blotting were used to detect DDP-induced changes. Thereafter, xenograft mouse models were constructed to verify the in vitro results. Obviously elevated HOXA11-AS and reduced miR-454-3p were found in NPC tissue and cisplatin-resistant NPC cells. Compared to the control cells, cells in the si-HOXA11-AS group showed sharp decreases in cell viability and IC₅₀, and these results were reversed in the miR-454-3p inhibitor group. Furthermore, HOXA11-AS targeted miR-454-3p, which further targeted c-Met. In comparison with cells in the control group, HNE1/DDP and C666-1/DDP cells in the si-HOXA11-AS group demonstrated fewer colonies, with an increase in the apoptotic rate, while the expression levels of c-Met, p-Akt/Akt and p-mTOR/mTOR decreased. Moreover, the si-HOXA11-AS-induced enhancement in sensitivity to cisplatin was abolished by miR-454-3p inhibitor transfection. The in vivo experiment showed that DDP in combination with si-HOXA11-AS treatment could inhibit the growth of xenograft tumors. Silencing HOXA11-AS can inhibit the c-Met/AKT/mTOR pathway by specifically upregulating miR-454-3p, thus promoting cell apoptosis and enhancing the sensitivity of cisplatin-resistant NPC cells to cisplatin.

• 치위생과학회지
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• 제13권3호
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• pp.321-329
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• 2013

과산화수소는 치아미백에 널리 사용되는 물질로 과다 사용시 치수세포에 손상을 일으킬 수 있다. 본 연구의 목적은 활성산소인 과산화수소에 의해 유도되는 상아모세포의 단계별 분화와 LOX isoforms과의 관계를 밝히고자 하였다. 치수세포에 분화유도 배지와 과산화수소를 시간과 농도별로 처리한 후 LOX 유전자 발현은 RT-PCR로 측정하였고, LOX enzyme activity는 고감도 형광분석으로 확인하였다. 또한 가장 많은 발현억제를 보인 LOX와 LOXL을 선택하여 siRNA 처리 후 분화표지자의 발현변화와 LOX enzyme activity를 확인하였다. 1. 과산화수소 처리에 따라 LOX, LOXL, LOXL3 mRNA 발현은 농도와 시간 의존적으로 감소하였으나 LOXL2와 LOXL4 mRNA는 변화가 없었다. 2. 과산화수소 처리된 LOX enzyme activity는 0.3 mM과 24시간에서 가장 많은 증가를 보였다. 3. ALP, OPN, OCN의 mRNA 발현은 LOX와 LOXL siRNAs 모두에서 억제되었고, DMP1과 DSPP는 LOX siRNA에서 더 많은 억제 효과를 보였다. 하지만, 분화단계별(초기, 중기, 말기) 차이는 보이지 않았다. 4. LOX와 LOXL siRNAs를 처리하여 LOX enzyme activity를 측정한 결과 LOX siRNA를 처리한 실험군에서 더 많은 억제효과를 보였다. 이러한 결과는 상아모세포 성장과 분화과정에 낮은 농도의 과산화수소가 분화를 유도하고 여기에 LOX가 관련됨을 알 수 있었다. 결론적으로, 과산화수소는 LOX 유전자 발현조절을 통해 치수세포의 성장과 분화에서 중추적인 역할을 할 것이라고 생각된다.

• Reproductive and Developmental Biology
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• 제31권4호
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• pp.241-248
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• 2007

Epigenetic modification dependent DNA methyltransferases (DNMTs) play an important role in tissue- and stage-specific gene regulation and normal mammalian development. In this study, we show that DNMTs are expressed at different levels during hematopoietic stem cell (HSC) differentiation to proerythrocytes. DNMT1, DNMT3A, and DNMT3B were highly expressed at day 7 after differentiation. We used specific siRNA as a tool to probe the relationship between the expression of DNMTs and erythropoietic differentiation. When introduced siRNA of DMNT1 and DMNT3b in human $CD34^+$ cells, these more differentiated into erythrocytes. This was confirmed by glycophorin A (GPA) positive cell analysis and globin gene expression. $GPA^+$ cells increased up to $20{\sim}30%$, and ${\gamma}$- and ${\epsilon}$-globin genes increased in siRNA transfected cells. Therefore, our data suggest that suppression of DNA methylation can affect positively differentiation of HSC and may contribute to expression of erythrocyte lineage genes including GPA and globins.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1099-1104
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• 2014

Aims and Background: Ginsenoside Rh2, which exerts the potent anticancer action both in vitro and in vivo, is one of the most well characterized ginsenosides extracted from ginseng. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between ginsenoside Rh2 and phosphoglucose isomerase/autocrine motility factor (PGI/AMF). Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PGI/AMF was assessed by western blot analysis and reverse transcription- PCR (RT-PCR) assay after transfection of a small interfering (si)-RNA to silence PGI/AMF. The effect of PGI/AMF on proliferation was measured by typan blue assay and antibody array. A cell counting kit (CCK)-8 and flow cytometry (FCM) were adopted to investigate the effects of Rh2 on PGI/AMF. The relationships between PGI/AMF and Rh2 associated with Akt, mTOR, Raptor, Rag were detected by western blot analysis. Results: KG1${\alpha}$ cells expressed PGI/AMF and its down-regulation significantly inhibited proliferation. The antibody array indicated that the probable mechanism was reduced expression of PARP, State1, SAPK/JNK and Erk1/2, while those of PRAS40 and p38 were up-regulated. Silencing of PGI/AMF enhanced the sensibility of $KG1{\alpha}$ to Rh2 by suppressing the expression of mTOR, Raptor and Akt. Conclusion: These results suggested that ginsenoside Rh2 suppressed the proliferation of $KG1{\alpha}$, the same as down-regulation of PGI/AMF. Down-regulation of PGI/AMF enhanced the pharmacological effects of ginsenoside Rh2 on KG1${\alpha}$ by reducing Akt/mTOR signaling.