• Journal of Animal Science and Technology
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• v.53 no.2
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• pp.171-176
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• 2011

Two trials at different body weights of Hanwoo heifers (average body weight of 143 and 257 kg, respectively) were conducted to determine crude protein requirements for maintenance (CPm). Six Hanwoo heifers in each trial were used in two 3 ${\times}$ 3 Latin square design with three diets containing three levels of CP, 14 days in each period. In trial 1, the diets were based on 2.8 kg fresh wt./day/heifer timothy hay (LCP) with supplements of either 250 g ground corn and 150 g corn gluten meal (MCP) or 500 g ground corn and 300 g corn gluten meal (HCP). In trial 2, the diets were based on 4.8 kg fresh wt./day/heifer timothy hay (LCP) with supplements of either 350 g ground corn and 250 g corn gluten meal (MCP) or 700 g ground corn and 500 g corn gluten meal (HCP). In trial 1, CP intakes were 236.6, 340.1, and 459.8 g/d for LCP, MCP, and HCP, respectively. Crude protein balances were 0.51, 1.87 and 3.20g/$BW^{0.75}$/d for LCP, MCP, and HCP, respectively. In trial 2, CP intakes were 415.2, 606.9 and 793.0g/d for LCP, MCP and HCP, respectively. Crude protein balances were 0.67, 1.03, 2.99 g/$BW^{0.75}$/d for LCP, MCP, and HCP, respectively. The maintenance requirements for CP from the regression equation between CP intake and CP balance were 4.58g/$BW^{0.75}$/d (trial 1) and 5.02 g/$BW^{0.75}$/d (trial 2) and lower than the value (5.56 g/$BW^{0.75}$/d) adopted by Korean Feeding Standards for Hanwoo (2007).

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.41-48
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• 1994

This experiment was carried out in order to evaluate the chemical composition and rumen degradation characteristics of Italian ryegrass harvested at three different growth stages, i,e. pre-blooming, early-blooming and late-blooming. Degradation values were obtained by incubation of the samples using the nylon bag technique on the rumen of sheep fed a normal diet (Timothy hay with 200 g/d concentrate per head) for 12, 24, 36, 48 and 72 hours, respectively. Neutral detergent fiver (NDF) content was highest at late-blooming (64.4%) while no difference was found among the pre-blooming and early-blooming (49.4% vs 48.3%). However, acid detergent fiber (ADF) content markedly increased from 30.0% at pre-blooming to 35.4% and 46.4% at early-blooming and late-blooming, respectively. Lignin and silica contents also increased as advancing maturity of the grass, Rumen degradation of dry matter (DM) significantly reduced (p < 0.05) as advancing maturity of the grass. Ruman degradation cellulose and ADF at pre-blooming were significantly higher (p < 0.05) than those of early-blooming and late-blooming. However, no significant differences were observed among the early-blooming and late-blooming. With advancing maturity, rumen degradation of NDF and hemicellulose significantly reduced (p < 0.05) at the incubation times.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.378-384
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• 1997

The ruminal solubility of copper (Cu), manganese (Mn), molybdenum (Mo), and zinc (Zn) in eight Philippine forages was studied. These forages were: 1) grasses: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), and napiergrass (Pennisetum purpureum Schumach.); 2) creeping legumes: calopogonium or calopo (Calopogonium muconoides Desv.) and centrocema (Centrocema pubescens Benth.); 3) tree lequmes: gliricidia or kakawate (Gliricidia sepium (Jacq.) Walp.), leucaena or ipil-ipil (Leucaena leucocephala (Lam.) de Wit.), and sesbania or katuray (Sesbania grandiflora (L.) Poir). Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72h in three rumen cannulated sheep fed with timothy hay (Phleum pratense L.) at 2% body weight/d. The 0-h bags were washed with deionized water. There were species differences (p < 0.05) on the different solubilities of trace elements. At O-h incubation, the trend of solubility was Mo (54%) > Zn (43%) > Cu (38.7%) > Mn (29.5%). At 3-h incubation, all the elements except Mn had an average solubility above 50%. Combining particulate passage rate (1.9%/h) and various trace elements disappearance rates, the effective ruminal solubilization (ERS) of trace elements were computed. The ERS across species ranged from 44.6 to 89.9% for Cu, 29.9 to 84% for Mn, 66 to 95.1% for Mo, and 30.1 to 82.3% for Zn resulting to a trend of Mo > Zn > Cu > Mn.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.18-26
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• 1994

Forage quality evaluation with several grasses grown at a high altitude pasture situated at 1,000m above sea level was made to determine what is the feed value of these grasses and whether these grasses have any differences in forage quality compared with the grdsses grown at lowland pastures. In this experiment, Common, Venture, and Palaton reed canarygrass (Phahris arundinucea L.) and Climax timothy (Phleum pratense L.) were used and harvested at two different dates, 5 July and 30 September, 1989. Cmde protein (CP) concentrations in the grasses grown in September were higher than in July. Among the grasses grown, Palaton and Common reed canarygrass were highest in CP concentration in July and September, respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) concentrations in the grasses harvested in July were higher than in September. Palaton reed canruygrass was lowest in ADF and NDF concentrations at the both harvest dates. Relative feed value (RFV) in the grases harvested in July was ranged from 92 to 105 and that in September was from 110 to 117. Palation reed canarygrass had the highest RFV of 105 and 117 in July and September, respectively, therefore, the forage quality of this grass was ranked as Grade 2 based on the AFGC Hay Quality Standards. Overall forage quality of the grasses grown at a high altitude pasture seemed to be higher than the forage quality at lowland pastures. Based on the RFV assigned by the AFGC, forages with RFV 92 to 117 in this experiment could only work well in rations for low producing dairy cows.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.71-79
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• 2018

Objective: Gelidium amansii (Lamouroux) is a red alga belonging to the family Gelidaceae and is commonly found in the shallow coasts of many East Asian countries, including Korea, China, and Japan. G. amansii has traditionally been utilized as an edible alga, and has various biological activities. The objective of this study was to determine whether dietary supplementation of G. amansii could be useful for improving ruminal fermentation. Methods: As assessed by in vitro fermentation parameters such as pH, total gas, volatile fatty acid (VFA) production, gas profile (methane, carbon dioxide, hydrogen, and ammonia), and microbial growth rate was compared to a basal diet with timothy hay. Cannulated Holstein cows were used as rumen fluid donors and 15 mL rumen fluid: buffer (1:2) was incubated for up to 72 h with four treatments with three replicates. The treatments were: control (timothy only), basal diet with 1% G. amansii extract, basal diet with 3% G. amansii extract, and basal diet with 5% G. amansii extract. Results: Overall, the results of our study indicate that G. amansii supplementation is potentially useful for improving ruminant growth performance, via increased total gas and VFA production, but does come with some undesirable effects, such as increasing pH, ammonia concentration, and methane production. In particular, real-time polymerase chain reaction indicated that the methanogenic archaea and Fibrobacter succinogenes populations were significantly reduced, while the Ruminococcus flavefaciens populations were significantly increased at 24 h, when supplemented with G. amansii extracts as compared with controls. Conclusion: More research is required to elucidate what G. amansii supplementation can do to improve growth performance, and its effect on methane production in ruminants.

• Journal of Animal Science and Technology
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• v.62 no.4
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• pp.485-494
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• 2020

Limiting amino acids (AAs) for milk production in dairy cows fed on a concentrate diet of corn grain and soybean meal was evaluated in this study. Four lactating and multiparous Holstein cows (in third or fourth parities, with an average body weight of 633 ± 49.2 kg), 8 to 9 weeks into their lactation period, were used in a 4 × 4 Latin square design. The experiment comprised four dietary treatments: (1) no intravenous infusion (control); (2) control plus intravenous infusion of an AA mixture of 6 g/d methionine, 19.1 g/d lysine, 13.8 g/d isoleucine, and 15.4 g/d valine (4AA); (3) control plus intravenous infusion of the AA mixture without methionine (no-Met); and (4) control plus intravenous infusion of the AA mixture without lysine (no-Lys). All animals were fed on a controlled diet (1 kg/d alfalfa hay, 10 kg/d silage, 14 kg/d concentrate mixture, ad libitum timothy hay). The AA composition of the diet and blood were determined using an automatic AA analyzer. Milk composition (protein, fat, lactose, urea nitrogen, and somatic cell counts) was determined using a MilkoScan. The results showed that feed intake for milk production did not differ from that of intravenous infusion using a limiting AA mixture. The 4AA treatment numerically had the highest milk yield (32.4 kg/d), although there was no difference when compared with the control (31.2 kg/d), no-Met (31.3 kg/d), and no-Lys (31.7 kg/d) treatments. The concentration of AAs in blood plasma of cows in all treatments, mainly isoleucine and valine, increased significantly compared with that of control. The no-Met treatment increased (p < 0.05) the concentration of lysine in the blood relative to the control and no-Lys treatments, whereas the no-Lys treatment increased (p < 0.05) the concentration of methionine relative to the control and no-Met treatments. In conclusion, milk production increased when feeding 10 g/d methionine to the cows, together with their concentrate diet of corn grain and soybean meal.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1683-1689
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• 2001

The levels of urea nitrogen both in blood (BUN) and milk (MUN), and milk protein (MP) reflect protein and energy intake in dairy herd feeding. Blood and milk constituents may be changes rhythmically and influence by different sampling time within a day and after feeding. Trials were conducted using five dietary treatments in both lactating and dry cows to study the effects of sampling time on concentrations of BUN, MUN and whole blood ammonia nitrogen (BAN) in practical dairy cow feeding in Taiwan. The conventional feed ingredients and forages including corn silage, alfalfa hay, timothy or pangola hay and corn grain were used as major source of the diet to follow practical dairy cow feeding. Five different diets were varying in amounts (low=L; standard=S; high=H) of crude protein (P) and energy (E) according to the NRC (1989). The energy to protein ratios in kcal/kg for the PSES, PLES, PHES, PSEH and PSEL were 10.82, 12.54, 9.41, 12.53 and 9.13 in lactating cows, and 11.38, 13.33, 9.78, 13.28 and 9.74 in dry cows, respectively. Results showed that after feeding at 9:30, BUN reached peak at 13:30 and was significantly higher than those to that sampled at 14:30 to 18:30 (p<0.05) in dry cows. Therefore the best blood sampling time for urea nitrogen assay in dry cows is 4 hours after morning feeding. In lactating cows, BUN of 13:30 was significantly higher than those of 8:30 to 11:30 (p<0.05), but there were no significant difference between the BUN values of other sampling time. Hence the suitable blood sampling time for BUN value in lactating cows was located on 3 to 8 hours after morning feeding, but the best time was 4 hours after morning feeding. MUN content is significantly higher in the afternoon collected bulk milk than the fore-strip morning milk (p<0.05), therefore the best sampling time for MUN is from afternoon collected bulk milk. Diurnal BAN changed without traceable rhythmic pattern and was negatively correlated to the BUN (r = -0.78). It is suggested that BAN may not be a good indicator for monitoring dairy cow feeding.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.83-89
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• 1994

The effects of inorganic selenium (Se), selenate and selenite on Se balance levels in the different ruminal fluid fractions were studied using Japanese Corriedale wethers with an average body weight of 47 kg. A $3{\times}3$ Latin square design was used with three animal, three periods and three treatments. In each period, there was 7 d dietary adjustment followed by 5 d total collection of urine and feces. Ruminal fluid samples were obtained at 0, 1, 3, 5 and 7 h postprandially on the final day of the collection period. The three dietary treatments were: (1) without Se supplementation (control); (2) with Se supplement as sodium selenate; and (3) sodium selenite at a rate of 0.2 mg Se/kg dietary DM. The basal diet was timothy hay (Phleum pratense L.) fed 2% of body weight/d. Results indicated that Se balance were higher (p < 0.05) for those animals under supplementation than those animals under control. Overall data gathered showed a similar digestion balance of selenate and selenite in sheep. Inorganic Se, both selenate and selenite produced positive Se contents of the ruminal feed particles and protozoa. Bacterial Se increased (p < 0.05) on the first three hours post-prandially in Se supplemented diets. Gross ruminal fluid fraction, although there was improvement on their Se content under the supplemented diets, the changes were insignificant over the control. free inorganic Se and Se in soluble protein of the ruminal fluid were not significantly different for selenate and selenite. Most of the Se in the ruminal fluids of the animals under supplementation were insoluble, indicating the influence of rumen environments on Se bioavaliability.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.54-62
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• 2018

Objective: Due to the threat of global warming, the livestock industry is increasingly interested in exploring how feed additives may reduce anthropogenic greenhouse gas emissions, especially from ruminants. This study investigated the effect of Rhodophyta supplemented bovine diets on in vitro rumen fermentation and rumen microbial diversity. Methods: Cannulated Holstein cows were used as rumen fluid donors. Rumen fluid:buffer (1:2; 15 mL) solution was incubated for up to 72 h in six treatments: a control (timothy hay only), along with substrates containing 5% extracts from five Rhodophyta species (Grateloupia lanceolata [Okamura] Kawaguchi, Hypnea japonica Tanaka, Pterocladia capillacea [Gmelin] Bornet, Chondria crassicaulis Harvey, or Gelidium amansii [Lam.] Lamouroux). Results: Compared with control, Rhodophyta extracts increased cumulative gas production after 24 and 72 h (p = 0.0297 and p = 0.0047). The extracts reduced methane emission at 12 and 24 h (p<0.05). In particular, real-time polymerase chain reaction analysis indicated that at 24 h, ciliate-associated methanogens, Ruminococcus albus and Ruminococcus flavefaciens decreased at 24 h (p = 0.0002, p<0.0001, and p<0.0001), while Fibrobacter succinogenes (F. succinogenes) increased (p = 0.0004). Additionally, Rhodophyta extracts improved acetate concentration at 12 and 24 h (p = 0.0766 and p = 0.0132), as well as acetate/propionate (A/P) ratio at 6 and 12 h (p = 0.0106 and p = 0.0278). Conclusion: Rhodophyta extracts are a viable additive that can improve ruminant growth performance (higher total gas production, lower A/P ratio) and methane abatement (less ciliateassociated methanogens, Ruminococcus albus and Ruminococcus flavefaciens and more F. succinogenes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.238-244
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• 2015

Three growing wethers were used to investigate the differences in nitrogen (N) retention, blood plasma metabolite concentration and energy-yielding nutrient supply to muscle and adipose tissue. The wethers were fed one of three diets: timothy hay with concentrate (THD), Italian ryegrass with concentrate (IRD), and rice straw with concentrate (RSD) for 11 days. The experimental diets were adjusted to the animals to provide 100 g of daily gain. The triglyceride (TG) concentration of blood plasma in arterial and portal veins was higher with THD and IRD than with RSD. Conversely, the available amount of TG in tissues was higher with IRD. The daily amount of glucose and non-esterified fatty acids (NEFA) supplied to muscle tissue and adipose tissue was numerically higher with THD than IRD or RSD. Although N retention did not differ among the diets, it was numerically higher with THD than with IRD or RSD. The results suggest that the difference in the amount of glucose and NEFA delivered to muscle tissue may reflect the N retention in response to forage based diets.