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Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation (벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석)

  • Park, Young-hie
    • Journal of Life Science
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    • v.31 no.4
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    • pp.385-388
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    • 2021
  • Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.

Design of bivariate step-stress partially accelerated degradation test plan using copula and gamma process

  • Srivastava, P.W.;Manisha, Manisha;Agarwal, M.L.
    • International Journal of Reliability and Applications
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    • v.17 no.1
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    • pp.21-49
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    • 2016
  • Many mechanical, electrical and electronic products have more than one performance characteristics (PCs). For example the performance degradation of rubidium discharge lamps can be characterized by the rubidium consumption or the decreasing intensity the lamp. The product may degrade due to all the PCs which may be independent or dependent. This paper deals with the design of optimal bivariate step-stress partially accelerated degradation test (PADT) with degradation paths modelled by gamma process. The dependency between PCs has been modelled through Frank copula function. In partial step-stress loading, the unit is tested at usual stress for some time, and then the stress is accelerated. This helps in preventing over-stressing of the test specimens. Failure occurs when the performance characteristic crosses the critical value the first time. Under the constraint of total experimental cost, the optimal test duration and the optimal number of inspections at each intermediate stress level are obtained using variance optimality criterion.

전장품의 신뢰성 향상을 위한 HALT기법 연구

  • Lee, Hui-Bok;Wi, Sin-Hwan;Park, Dong-Gyu
    • Proceedings of the Korean Reliability Society Conference
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    • 2011.06a
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    • pp.55-60
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    • 2011
  • Recently, the application of electronics in vehicle is increasing, in order to assess the reliability of the electronics, highly accelerated life test is used, highly accelerated life test can assess the reliability of the electronics in the short time. In this study, optimized HALT technique can be applied to the electronics is proposed. The main results are as follows; i) HALT is proceed to the 8-step process. ii) The test mode of HALT is composed of the cold step stress, hot step stress, vibration step stress and combined environments stress. iii) The time dwell is set to at least 20 minutes.

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A New Procedure for the Initial Solution of Goal Programming (목표계획법 초기해의 새로운 절차에 관한 연구)

  • ;;Choi, Jae Bong
    • Journal of the Korean Operations Research and Management Science Society
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    • v.19 no.1
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    • pp.113-122
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    • 1994
  • This study proposes a new procedure to find an initial solution to reduce the number of iterations of goal programming. The process of computing an initial solution is divided into two steps in this study. Decision variables which satisfy feasibility using Gaussian eliminations construct an initial solution reducing the iterations in the first step. It uses LHS as a tool that decision variables construct an initial solution. The initial solution which is constructed by the first step computes the updated coefficient of the objective function in the second step. If the solution does not satisfy the optimality, the optimal solution using the Modified Simplex Method is sought. The developed method doesn't reduce the overall computing time of goal programming problems, because time is more required for the process of constructing an initial solution. But The result of this study shows that the proposed procedure can reduce the large number of iterations in the first step effectively.

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Stability Analysis of Kalman Filter by Orthonormalized Compressed Measurement

  • Hyung Keun Lee;Jang Gyu Lee
    • KIEE International Transaction on Systems and Control
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    • v.2D no.2
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    • pp.97-107
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    • 2002
  • In this paper, we propose the concept of orthonormalized compressed measurement for the stability analysis of discrete linear time-varying Kalman filters. Unlike previous studies that deal with the homogeneous portion of Kalman filters, the proposed Lyapunov method directly deals with the stochastically-driven system. The orthonorrmalized compressed measurement provides information on the a priori state estimate of the Kalman filter at the k-th step that is propagated from the a posteriori state estimate at the previous block of time. Since the complex multiple-step propagations of a candidate Lyapunov function with process and measurement noises can be simplified to a one-step Lyapunov propagation by the orthonormalized compressed measurement, a stochastic radius of attraction can be derived that would be impractically difficult to obtain by the conventional multiple-step Lyapunov method.

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Analysis of Step Discontinuity Microstrip Line Using SCN-TLM Method (SCN-TLM법을 이용한 스텝 불연속 마이크로스트립 라인 해석)

  • Kim, T.W.;Shin, Y.J.;Kim, Y.S.;Lee, S.H.
    • Proceedings of the IEEK Conference
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    • 2007.07a
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    • pp.477-478
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    • 2007
  • The SCN-TLM method presented in this paper is another independent approaches for obtaining frequency domain results for microstrip line. The structure analysed with this TLM algorithm is step discontinuity microstrip line and the symmetrical condensed node is used. After numerical analysis, the frequency dependent scattering parameters of a step discontinuity microstrip line have been calculated by Fourier transform of the time domain data. From the time domain TLM numerical results, this numerical analysis is shown to be an efficient method for modelling complicated structure as step discontinuity microstrip line.

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A Study of PSs Modeling of Ulchin N/P #1 by AVR Step Test (AVR 스템시험에 의한 울진 N/P 1호기 PSS 모델링 연구)

  • 김동준;문영환;전동훈;김태균
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.50 no.8
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    • pp.351-358
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    • 2001
  • This paper deals with the PSS modeling of Ulchin N/P #1 as well as the generator and excitation system modeling by utilizing the recorded data from AVR step test, which has been performed by entering small voltage signal into the AVR summing point. In addition to it. two recorded results obtained from the AVR step test with PSS sunning and without PSS running have not only been compared each other on the time domain, but also they heve been analyzed with FFT analysis on the frequency domain; thus, the desirable effects of running PSS in Ulchin N/P #1 on power system have been explicitly confirmed. Finally, the derived PSS model parameters lead to good matches between simulation results and recorded data.

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A fourth order finite difference method applied to elastodynamics: Finite element and boundary element formulations

  • Souza, L.A.;Carrer, J.A.M.;Martins, C.J.
    • Structural Engineering and Mechanics
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    • v.17 no.6
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    • pp.735-749
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    • 2004
  • This work presents a direct integration scheme, based on a fourth order finite difference approach, for elastodynamics. The proposed scheme was chosen as an alternative for attenuating the errors due to the use of the central difference method, mainly when the time-step length approaches the critical time-step. In addition to eliminating the spurious numerical oscillations, the fourth order finite difference scheme keeps the advantages of the central difference method: reduced computer storage and no requirement of factorisation of the effective stiffness matrix in the step-by-step solution. A study concerning the stability of the fourth order finite difference scheme is presented. The Finite Element Method and the Boundary Element Method are employed to solve elastodynamic problems. In order to verify the accuracy of the proposed scheme, two examples are presented and discussed at the end of this work.

Graft Polymerization of Methyl Methacrylate onto Cotton Fiber -Comparison of two step graft polymerization and emulsion graft polymerization- (면섬유에 Methyl Methacrylate의 그라프트중합 - 이단계 그라프트중합과 유화 그라프트중합의 비교 -)

  • Bae Hyun-Sook;Kim Sung-Reon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.13 no.1 s.29
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    • pp.89-97
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    • 1989
  • Graft polymerization of MMA onto cotton fiber was carried out in two ways, two step graft polymerization and one step emulsion graft polymerization, using tetravalent cerium ion as an initiator. At two step graft polymerization, the first step was the pretreatment of cotton fiber with an aqueous initiator solution and the second was the grafting pretreated cotton fiber in the monomer solution. In case of one step emulsion graft polymerization, MMA was emulsified with SLS in initiator solution. Under the various graft polymerization conditions, graft yield, graft efficiency and from the Arrhenius plot the apparent activation energy were compared. The results of this study were as follows: 1. Graft yield and graft efficiency of emulsion graft polymerization were higher than those of two step graft polymerization. 2. In case of two step graft polymerization, graft yield was affected by the pretreatment time of cotton fiber with an aqueous initiator solution. And graft yield of emulsion graft polymerization was increased with the concentration of emulsifier below cmc of SLS and was decreased thereafter. 3. Elevation of temperature resulted increase in graft yield for both grafting methods. The apparent activation energy of emulsion graft pelymerzation was lower than that of two step graft polymerization. 4. Increased reaction time increased in graft yield, but decreased in graft efficiency. 5. Moisture regain of grafted cotton was decreased with graft yield.

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An acoustic channel estimation using least mean fourth with an average gradient vector and a self-adjusted step size (기울기 평균 벡터를 사용한 가변 스텝 최소 평균 사승을 사용한 음향 채널 추정기)

  • Lim, Jun-Seok
    • The Journal of the Acoustical Society of Korea
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    • v.37 no.3
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    • pp.156-162
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    • 2018
  • The LMF (Least Mean Fourth) algorithm is well known for its fast convergence and low steady-state error especially in non-Gaussian noise environments. Recently, there has been increasing interest in the LMS (Least Mean Square) algorithms with self-adjusted step size. It is because the self-adjusted step-size LMS algorithms have shown to outperform the conventional fixed step-size LMS in the various situations. In this paper, a self-adjusted step-size LMF algorithm is proposed, which adopts an averaged gradient based step size as a self-adjusted step size. It is expected that the proposed algorithm also outperforms the conventional fixed step-size LMF. The superiority of the proposed algorithm is confirmed by the simulations in the time invariant and time variant channels.