• Journal of Periodontal and Implant Science
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• v.31 no.4
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• pp.787-801
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• 2001

The molecular mechanisms control the function of PDL(periodonta1 ligament) cells and/or fibroblasts remain unclear. PDLsl7, PDL-specific gene, had previousely　identified the cDNA for a novel protein from cultured　PDL fibroblasts using subtraction hybridization between gingival fibroblasts and　PDL fibroblasts. The purpose　of this study was to determine the regulation by growth factors and cytokines on　PDLsl7 gene expression in　cultured human periodontal ligament cells and observe the immunohistochemical　localization of PDLsl7 protein in various tissues of mouse. Primary PDL fibroblasts isolated by scraping the root of the extracted human mandibular third molars. The cells were incubated with various concentration of human recombinant $IL-1{\beta}$, PDGF-BB and TGF\;${\beta}$ for 48h nd 2 weeks. At each time point total RNA was extracted and the levels of transcription ere assessed by reverse transcription-polymerase chain reaction (RT-PCR assay). polyclonal antiserum raised against PDLsl7 peptides, CLSVSYNRSYQINE and SEAVHETDLHDGC, were made, and stained the tooth, periodontium, developing bone, bone marrow and mid-palatal suture of the mouse. The results were as follows. 1. PDLsl7 mRNA levels were increased in response to PDGF (10ng/ml) and $TGF\;{\beta}$(20ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF{\beta}$for 48 h. 2. PDLsl7 was up-regulated only by $TGF{\beta}$(20 ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF\;{\beta}$ for 2 weeks and unchanged by the other stimulants. 3. PDLsl7 was a novel protein coding the 142 amino acid peptides in the ORF and the nucleotide sequences of the obtained cDNA from RT-PCR was exactly same as the nucleotides of the database. 4. Immunohistochemical analysis showed that PDLsl7 is preferentially expressed in the PDL, differentiating osteoblast-like cells and stromal cells of the bone marrow in the adult mouse. 5. The expression of PDLsl7 protein was barely detectable in gingival fibroblasts, hematopoetic cells of the bone marrow and mature osteocytes of the alveolar bone. These results suggest that PDLsl7 might upregulated by PDGF-BB or $TGF{\beta}$ and acts at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL differentiate into multiple cell types. However, more research needs to be performed to gain a better understanding of the exact function of PDLsl7 during the differentiation of bone marrow mesenchymal and PDL cells.

• Journal of the Korean Society for Railway
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• v.20 no.2
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• pp.165-172
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• 2017

High frequency squeal noise can be generated when a railroad vehicle runs a sharp curved section; this noise causes environmental complaints and excessive wear on the wheel and the railroad track. In this paper, curved squeal noise experiments on a commercial railway were carried out to investigate this phenomenon. The relationship of the squeal noise pressure level, the frequency characteristics, the railway running speed, and the modal behavior of the wheel were investigated. At the same time, the lateral motion of the wheel relative to the rail was captured using a video camera; wheel movement was calculated when the noise was generated. queal noise occurred at the highest level at the inner front wheel; this phenomena is considered to be related to the lateral vibration response characteristics of the wheel. It can be seen that the magnitude of this squeal noise is not directly related to the increase in vehicle speed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.5
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• pp.455-463
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• 2012

In this study, we calculate dynamic constrained force of tower top and blade root of a floating offshore wind turbine. The floating offshore wind turbine is multibody system which consists of a floating platform, a tower, a nacelle, and a hub and three blades. All of these parts are regarded as a rigid body with six degree-of-freedom(DOF). The platform and the tower are connected with fixed joint, and the tower, the nacelle, and the hub are successively connected with revolute joint. The hub and three blades are connected with fixed joint. The recursive formulation is adopted for constructing the equations of motion for the floating wind turbine. The non-linear hydrostatic force, the linear hydrodynamic force, the aerodynamic force, the mooring force, and gravitational forces are considered as external forces. The dynamic load at the tower top, rotor shaft, and blade root of the floating wind turbine are simulated in time domain by solving the equations of motion numerically. From the simulation results, the mutual effects of the dynamic response between the each part of the floating wind turbine are discussed and can be used as input data for the structural analysis of the floating offshore wind turbine.

• Journal of the Ergonomics Society of Korea
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• v.32 no.6
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• pp.481-487
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• 2013

Objective: The present study aims to evaluate the visual reactions of users when they are playing games of different flow levels, and to explore the visual variables that can sensitively reflect the different flow levels. Background: The flow is defined as a psychological state where interface users feel their actions in a virtual setting identical to those in real environment. To measure the flow states of users, the questionnaire-based FSS(Flow State Scale) has mostly been used. However, this method is a qualitative test that has limits in terms of the accuracy of users' flow experiences. Therefore, more accurate methods to measure users' flow experiences are required. Method: Ten subjects participated in the experiment, where the independent variables were three games with different flow levels(puzzle games, dot drawing and coloring) and the time frame(the first and last 10 seconds in game playing), whereas the dependent variables included the pupil size and the frequency and duration of eye blinking. This study was a within-subject design. Each participant performed three types of games with different flow levels 3 times for each for 10 minutes, and their visual reactions to each game were measured. Results: The higher the flow cause the bigger pupil size(p<0.01) and the lower eye blinking frequency(p<0.1), indicating that different types of games lead to different flow levels. The pupil size during the last 10 seconds when the flow level was higher was bigger by 2.1% compared with that during the first 10 seconds in game playing(p<0.1), and the eye blinking frequency decreased by 12%(p<0.01). Conclusion: It was found out that the pupil size and the frequency and duration of eye blinking were psychophysiological indices for evaluating users' flow experiences, which could quantify the flow states users go through. The psychophysiological variables capable of measuring diverse aspects of the flow need diversifying to be applicable to precise measurement of the flow. Application: These studies are warranted for both quantitative analysis of flow levels and qualitative improvement of cyber leisure in line with development of healthy games.

• Tuberculosis and Respiratory Diseases
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• v.56 no.4
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• pp.381-392
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• 2004

Arsenic trioxide($As_2O_3$) was introduced into the treatment of refractory or relapsed acute promyelocytic Ieukemia. Some investigators have reported that arsenic trioxide had induced apoptosis in a variety of solid human tumor cell lines, including non-small cell lung cancer. Non-steroidal anti-inflammatory drugs(NSAIDs) are powerful chemopreventive agents for gastrointestinal cancers and the growth of established tumors are reduced by inducing apoptosis. It's also reported that NSAIDs enhanced tumor response to chemotherapeutic drugs or radiation. In this study, we aimed to determine whether combination of arsenic trioxide with sulindac augmented its apoptotic potential in NCI-H157 human lung cancer cells. The human lung cancer cell line NCI-H157 was treated with arsenic trioxide and sulindac. Cell viability was measured by the MTT assay. Apoptosis was measured by nuclear staining and flow cytometric analysis. The catalytic activity of the caspase families were measured by the fluorogenic cleavage of biosubstrates. The western blotting were also performed to define the mechanical basis of apoptosis. Combination treatment of arsenic trioxide and sulindac decreased the viability of NCI-H157 human lung cancer cells in a dose-dependent manner. The catalytic activity of caspase-3, 8 and 9 proteases were increased after combination treatment. Consistently PARP was cleaved from 116kDa to 85kDa fragments, and the expression of ICAD was decreased by time-dependent manner. Also combination treatment increased the expression of Fas and Fas/L. Combination therapy of arsenic trioxide with sulindac augments cell death and induces apoptosis via the activation of caspase cascade in NCI-H157 human lung carcinoma cells.

• Geophysics and Geophysical Exploration
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• v.13 no.4
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• pp.349-356
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• 2010

Analysis of high-resolution seismic profiles acquired from the southeastern continental shelf of Korea reveals that the late Quaternary transgressive deposits consist of six seismic units created in response to sea-level rise. These units with different seismic facies and geometry can be grouped into two distinct depositional wedges (paralic and marine) bounded by a ravinement surface. The paralic component underlying the ravinement surface consists of the sediment preserved from shoreface erosion and contains incised-channel fill, ancient beach-shoreface deposit and estuarine deposit. The top of paralic unit is truncated by a ravinement surface and overlain by marine component. The marine component consists of the sediment produced through shoreface erosion during landward transgression and contains mid-shelf sand sheet, mid-shelf sand ridge and inner shelf sand sheet. Such transgressive stratigraphic architecture of six sedimentary units is controlled by a function of lateral changes in the balance among rates of relative sea-level rise, sediment input and marine processes at any given time.

• Journal of the Korea Society of Computer and Information
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• v.17 no.6
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• pp.163-172
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• 2012

Collaborative filtering which is used explicit method in a existing recommedation system, can not only reflect exact attributes of item but also still has the problem of sparsity and scalability, though it has been practically used to improve these defects. This paper proposes the personalized recommendation system using RFM method and k-means clustering in u-commerce which is required by real time accessablity and agility. In this paper, using a implicit method which is is not used complicated query processing of the request and the response for rating, it is necessary for us to keep the analysis of RFM method and k-means clustering to be able to reflect attributes of the item in order to find the items with high purchasablity. The proposed makes the task of clustering to apply the variable of featured vector for the customer's information and calculating of the preference by each item category based on purchase history data, is able to recommend the items with efficiency. To estimate the performance, the proposed system is compared with existing system. As a result, it can be improved and evaluated according to the criteria of logicality through the experiment with dataset, collected in a cosmetic internet shopping mall.

• Journal of Digital Convergence
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• v.11 no.9
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• pp.229-238
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• 2013

Out-of-hospital cardiac arrest is a major public health problem in Korea. The survival rate to discharge remains at approximately 3.5% and only 1% have good neurological function. To increase the survival rate, prehospital care should restore spontaneous circulation. The purpose of this study was to analyze the factors associated with return of spontaneous circulation(ROSC) after out-of-hospital cardiac arrest. Data used for this study were collected from KCDC Out-of-Hospital Cardiac Arrest Surveillance 2009. As for the results of decision tree analysis, it is clear that prehospital CPR, cardiac arrest witness, activity, past history(cancer/heart disease/stroke), place, bystander CPR, response time, age, etc are significant contributing factors in ROSC. Among 16 cardiac arrest types from decision tree classification, the ROSC rate of type 1 is the highest(29.6%). Also notable is the fact that bystander CPR was strongly correlated with ROSC of patents with cardiac arrest occurring in non-public places. Community resources should be concentrated on increasing bystander CPR and early prehospital emergency care.

• Geophysics and Geophysical Exploration
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• v.18 no.2
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• pp.39-53
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• 2015

Analysis of multi-channel seismic reflection profiles acquired from the southwestern margin of Ulleung Basin reveals that the cut and fill structures, which show U-shaped or V-shaped morphology, occur on variable size. The cut and fill structure mostly consists of fine-grained sediments on the well data and is characterized by transparent or semitransparent seismic facies on the seismic section. Such cut and fill structures dominantly occur in the syn-compressional megasequence (MSQ3), which was deposited during basin deformation of late Miocene, among the four megasequences of the study area. These cut and fill structures can be divided into three groups based on their size and formation time. The cut and fill structures of Group I were formed when Dolgorae structure was active, and occurred on a small scale. The cut and fill structures of group II were formed when both Dolgorae structure and Gorae V structure were active, and the number and size of those increased compared with group I. The cut and fill structures of group III were formed when Dolgorae structure was weaken gradually but Gorae V structure kept active, and the number and size of those decreased in comparison with group II. Consequently the cut and fill structures in the southwestern margin of Ulleung basin are interpreted as submarine canyon based on spatial distribution, size and fill sediment. They were controlled by the tectonic movement in response to basin closure and tectonic-induced sediment supply variation.

• Journal of Korea Water Resources Association
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• v.45 no.7
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• pp.657-674
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• 2012

This paper presents the effect of spatially-distributed rainfall on both rainfall-sediment-runoff and erosion or deposition in the experimental Cheoncheon catchment: upstream of Yongdam dam basin. The rainfall fields were generated by three rainfall interpolation techniques (Thiessen polygon: TP, Inverse Distance Weighting: IDW, Kriging) based only on ground gauges and two radar rainfall synthetic techniques (Gauge-Radar ratio: GR, Conditional Merging: CM). Each rainfall field was then assessed in terms of spatial feature and quantity and also used for rainfall-sediment-runoff and erosion-deposition simulation due to the spatial difference of rainfall fields. The results showed that all the interpolation methods based on ground gauges provided very similar hydrologic responses in spite of different spatial pattern of erosion and deposition while raw radar and GR rainfall fields led to underestimated and overestimated simulation results, respectively. The CM technique was acceptable to improve the accuracy of raw radar rainfall for hydrologic simulation even though it is more time consuming to generate spatially-distributed rainfall.