• ETRI Journal
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• 제33권4호
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• pp.633-636
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• 2011

In this letter, we propose an overlaid hybrid division duplex (HDD) concept for cellular systems which divides a cell into inner and outer regions and utilizes the merits of both time division duplex (TDD) and frequency division duplex (FDD). The proposed system can take advantage of both TDD and FDD without handover between two duplex schemes. Moreover, it is shown that the proposed HDD system outperforms the conventional TDD or FDD system with mobile relay stations when the synchronization issue is considered in orthogonal frequency division multiple access systems. Thus, the proposed overlaid HDD can be considered as a new framework for future cellular systems.

• 한국식품위생안전성학회지
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• 제26권2호
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• pp.114-119
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• 2011

본 연구에서는 신선편의 채소와 과일에서 주로 검출되는 대장균(E. coli Ol577:H7), 리스테리아(L. monocytogenes), 살모넬라(Salmonella, spp.), 형색포도상구균(S. aureus)을 검출하고 동정할 수 있는 real time PCR 방법을 melting curve 분석을 활용하여 single tube 반응으로 두 종의 식중독균을 동시 검출하는 방법을 개발하고자 하였다. 대장균의 ${\beta}$-glucuronidase (uidA), 황색포도상구균의 thermonuclease (nuc), 리스테리아의 hemolycin (hly), 살모넬라의 tetrahionate reductase (ttr) 를 특이적으로 검출할 수 있는 4종의 프라이머 세트에 대한 real-time PCR의 melting curve 분석을 통하여 황색포도상구균과 대장균 동시분석 시 $T_m$ 값이 $80.6{\pm}0.9^{\circ}C$와 $86.9{\pm}0.5^{\circ}C$, 리스테리아와 살모넬라 동시분석 시 Tm 값이 $80.4{\pm}0.6^{\circ}C$와 $88.1{\pm}0.11^{\circ}C$로 확인하였고, 그 결과 정제되어진 각 식중독균의 genomic DNA를 주형으로 한 duplex real-time PCR 방법이 uniplex real-time PCR 방법과 마찬가지로 10 genome equivalents 까지 검출할 수 있는 민감도를 나타내었다. 또한, 양배추에 네 종의 식중독균을 접종하고, 증균배양 없이 DNA를 추출하여 duplex real-time PCR 을 수행한 결과 모든 식종목균에서 $10^3$ CFU/g의 검출 한계를 나타내었다. 결과적으로 개발된 melting curve 분석을 이용한 duplex real-time PCR 방법은 식품안전 증진을 위한 시간, 노동력, 비용 절감에 있어서 유효한 방법이 될 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.630-636
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• 2013

Recently, there has been a growing body of evidence showing that cellular microRNAs (miRNAs) are involved in virus-host interactions. Numerous studies have focused on analyses of the expression profiles of cellular miRNAs, but the expression patterns of Dicer, which is responsible for the generation of miRNAs, have only rarely been explored in Gallus gallus. We developed a duplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for the relative quantification of the mRNAs of Dicer and ${\beta}$-actin in G. gallus. To apply this method, the expression of Dicer in avian cells after infection with avian leukosis virus subgroup J (ALV-J) was detected using our established duplex real-time RT-PCR. The duplex real-time RT-PCR assay is sufficiently sensitive, specific, accurate, reproducible, and cost-effective for the detection of Dicer in G. gallus. Furthermore, this study, for the first time, demonstrated that ALV-J can induce differential expression of Dicer mRNA in the ALV-J-infected cells.

• ETRI Journal
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• 제35권4호
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• pp.710-713
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• 2013

We propose a new space-time block coding (STBC) for asynchronous cooperative systems in full-duplex mode. The orthogonal frequency division multiplexing (OFDM) transmission technique is used to combat the timing errors from the relay nodes. At the relay nodes, only one OFDM time slot is required to delay for a pair-wise symbol swap operation. The decoding complexity is lower for this new STBC than for the traditional quasi-orthogonal STBC. Simulation results show that the proposed scheme achieves excellent performances.

• Journal of Communications and Networks
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• 제9권3호
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• pp.247-253
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• 2007

In this paper, downlink capacity of time duplex division (TDD) based cellular wireless networks utilizing fixed hopping stations is investigated. In the network, a number of fixed subscriber stations act as hopping transmission stations between base stations and far away subscribers, forming a cellular and ad hoc mobile network model. At the radio layer, TDD code division multiple access (CDMA) is selected as the radio interface due to high efficiency of frequency usage. In order to improve the system performance in terms of downlink capacity, we propose different time slot allocation schemes with the usage of fixed hopping stations, which can be selected by either random or distanced dependent schemes. Performance results obtained by computer simulation demonstrate the effectiveness of the proposed network to improve downlink system capacity.

• 한국축산식품학회지
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• 제37권4호
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• pp.599-605
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• 2017

Korean native honey (KNH) is much more expensive than European honey (EH) in Korea, because KNH is a favored honey which is produced less than EH. Food fraud of KNH has drawn attention of the government office concerned, which is in need of a method to differentiate between KNH and EH which are produced by the Asiatic honeybee, Apis cerana and the European honeybee, Apis mellifera, respectively. A method to discriminate KNH and EH was established by using duplex polymerase chain reaction (PCR) in this study. Immunochromatographic assay (IC) was examined to analyze the duplex PCR product. The DNA sequences of primers for the duplex PCR were determined by comparing cytochrome C oxidase genes of the two honey bee species. Chelex resin method was more efficient in extracting genomic DNA from honey than the other two procedures of commercial kits. The duplex PCR amplifying DNA of 133 bp were more sensitive than that amplifying DNA of 206 bp in detecting EH in the honey mixture of KNH and EH. Agarose gel electrophoresis and IC detected the DNA of 133 bp at the ratios of down to 1% and 5% EH in the honey mixture, respectively and also revealed that several KNH products distributed by internet shopping sites were actually EH. In conclusion, the duplex PCR with subsequent IC could also discriminate between KNH and EH and save time and labor.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권1호
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• pp.108-125
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• 2015

We propose a new time-division half-duplex estimate-and-forward (EF) relaying strategy suitable for a relay with mobility. We reconfigure EF relaying to guarantee a strong relay-destination link which is required to achieve a high rate using EF relaying. Based on the reconfigured model, we optimize the relaying strategy to attain a high rate irrespective of the relay position with preserving the total transmit bandwidth and energy. The proposed relaying strategy achieves high communication reliability for any relay position, which differs from conventional EF and decode-and-forward (DF) relaying schemes.

• 한국산학기술학회논문지
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• 제8권5호
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• pp.1076-1081
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• 2007

본 논문은 시분할 송수신 시스템에 사용될 수 있는 새로운 구조의 스위칭회로를 제안한다. 기존의 시분할 송수신 시스템에 사용되는 스위칭 회로의 경우 안테나, 송신단 그리고 수신단을 서큘레이터로 각 단자를 분리하였다. 이러한 기존의 방법은 서큘레이터 사용으로 인하여 회로전체의 가격을 증가시키는 원인이 되며, 원치 않는 상호 변복조 성분이 만들어져 전체 시스템의 악화로 이어지게 된다. 본 논문에서 제안하는 회로는 비선형 소자인 서큘레이터를 사용하지 않고 기본적인 가지선로 결합기의 한 쪽 선로를 결합선로로 등가 모델링하여 응용한 회로이다. 이 회로는 등가 모델링 된 결합선로의 격리 포트를 개방/단락의 종단조건을 이용하여 신호의 흐름을 제어하는 새로운 구조의 스위칭회로이다. 본 논문에서는 제시한 설계법의 타당성을 검증하기 위하여 Wibro 서비스 주파수대역인 2.3GHz에서 제작 및 측정하였다.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1398-1403
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• 2016

The simultaneous detection and accurate identification of hepatitis A virus (HAV) is critical in food safety and epidemiological studies to prevent the spread of HAV outbreaks. Towards this goal, a one-step duplex reverse-transcription (RT)-PCR method was developed targeting the VP1/P2B and VP3/VP1 regions of the HAV genome for the qualitative detection of HAV. An HAV RT-qPCR standard curve was produced for the quantification of HAV RNA. The detection limit of the duplex RT-PCR method was 2.8 × 10¹ copies of HAV. The PCR products enabled HAV genotyping analysis through DNA sequencing, which can be applied for epidemiological investigations. The ability of this duplex RT-PCR method to detect HAV was evaluated with HAV-spiked samples of fresh lettuce, frozen strawberries, and oysters. The limit of detection of the one-step duplex RT-PCR for each food model was 9.4 × 10² copies/20 g fresh lettuce, 9.7 × 10³ copies/20 g frozen strawberries, and 4.1 × 10³ copies/1.5 g oysters. Use of a one-step duplex RT-PCR method has advantages such as shorter time, decreased cost, and decreased labor owing to the single amplification reaction instead of four amplifications necessary for nested RT-PCR.

• 전자통신동향분석
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• 제30권3호
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• pp.123-130
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• 2015

본고는 TDD(Time Division Duplex)/FDD(Frequency-Division Duplex) 네트워크 컨버전스를 통해 LTE(Long Term Evolution) 서비스를 제공하고 있는 주요 사업자들의 동향에 대하여 분석 정리하였다. 본고의 분석 결과에 따르면, 상당수 사업자가 보유 주파수 여건을 고려하여 경쟁력을 높이기 위한 목적으로 네트워크 컨버전스를 추진한 것으로 나타났다. 향후 국내에서도 TDD/FDD 컨버전스를 촉진하고 주파수 이용 효율성을 개선하기 위한 정책방안에 대한 모색이 필요하다고 사료된다.