• ETRI Journal
• /
• v.33 no.4
• /
• pp.633-636
• /
• 2011

In this letter, we propose an overlaid hybrid division duplex (HDD) concept for cellular systems which divides a cell into inner and outer regions and utilizes the merits of both time division duplex (TDD) and frequency division duplex (FDD). The proposed system can take advantage of both TDD and FDD without handover between two duplex schemes. Moreover, it is shown that the proposed HDD system outperforms the conventional TDD or FDD system with mobile relay stations when the synchronization issue is considered in orthogonal frequency division multiple access systems. Thus, the proposed overlaid HDD can be considered as a new framework for future cellular systems.

• Journal of Food Hygiene and Safety
• /
• v.26 no.2
• /
• pp.114-119
• /
• 2011

In this study, two duplex real-time PCR approach with melting curve analysis is presented for the detection of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus, which are important food-borne bacterial pathogens usually present in fresh and/or minimally processed vegetables. Reaction conditions were adjusted for the simultaneous amplification and detection of specific fragments in the ${\beta}$-glucuronidase (uidA, E. coli), thermonuclease (nuc, S. aureus), hemolycin (hly, L. monocytogenes) and tetrathionate reductase (ttr, Salmonella spp.) genes. Melting curve analysis using a SYBR Green I real-time PCR approach showed characteristic $T_m$ values demonstrating the specific and efficient amplification of the four pathogens; $80.6{\pm}0.9^{\circ}C$, $86.9{\pm}0.5^{\circ}C$, $80.4{\pm}0.6^{\circ}C$ and $88.1{\pm}0.11^{\circ}C$ for S. aureus, E. coli O157:H7, L. monocytogenes and Salmonella spp., respectively. For all the pathogens, the two duplex, real-time PCR was equally sensitive to uniplex real-time PCR, using same amounts of purified DNA, and allowed detection of 10 genome equivalents. When our established duplex real-time PCR assay was applied to artificially inoculated fresh lettuce, the detection limit was $10^3$ CFU/g for each of these pathogens without enrichment. The results from this study showed that the developed duplex real-time PCR with melting curve analysis is promising as a rapid and cost-effective test method for improving food safety.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.5
• /
• pp.630-636
• /
• 2013

Recently, there has been a growing body of evidence showing that cellular microRNAs (miRNAs) are involved in virus-host interactions. Numerous studies have focused on analyses of the expression profiles of cellular miRNAs, but the expression patterns of Dicer, which is responsible for the generation of miRNAs, have only rarely been explored in Gallus gallus. We developed a duplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for the relative quantification of the mRNAs of Dicer and ${\beta}$-actin in G. gallus. To apply this method, the expression of Dicer in avian cells after infection with avian leukosis virus subgroup J (ALV-J) was detected using our established duplex real-time RT-PCR. The duplex real-time RT-PCR assay is sufficiently sensitive, specific, accurate, reproducible, and cost-effective for the detection of Dicer in G. gallus. Furthermore, this study, for the first time, demonstrated that ALV-J can induce differential expression of Dicer mRNA in the ALV-J-infected cells.

• ETRI Journal
• /
• v.35 no.4
• /
• pp.710-713
• /
• 2013

We propose a new space-time block coding (STBC) for asynchronous cooperative systems in full-duplex mode. The orthogonal frequency division multiplexing (OFDM) transmission technique is used to combat the timing errors from the relay nodes. At the relay nodes, only one OFDM time slot is required to delay for a pair-wise symbol swap operation. The decoding complexity is lower for this new STBC than for the traditional quasi-orthogonal STBC. Simulation results show that the proposed scheme achieves excellent performances.

• Journal of Communications and Networks
• /
• v.9 no.3
• /
• pp.247-253
• /
• 2007

In this paper, downlink capacity of time duplex division (TDD) based cellular wireless networks utilizing fixed hopping stations is investigated. In the network, a number of fixed subscriber stations act as hopping transmission stations between base stations and far away subscribers, forming a cellular and ad hoc mobile network model. At the radio layer, TDD code division multiple access (CDMA) is selected as the radio interface due to high efficiency of frequency usage. In order to improve the system performance in terms of downlink capacity, we propose different time slot allocation schemes with the usage of fixed hopping stations, which can be selected by either random or distanced dependent schemes. Performance results obtained by computer simulation demonstrate the effectiveness of the proposed network to improve downlink system capacity.

• Food Science of Animal Resources
• /
• v.37 no.4
• /
• pp.599-605
• /
• 2017

Korean native honey (KNH) is much more expensive than European honey (EH) in Korea, because KNH is a favored honey which is produced less than EH. Food fraud of KNH has drawn attention of the government office concerned, which is in need of a method to differentiate between KNH and EH which are produced by the Asiatic honeybee, Apis cerana and the European honeybee, Apis mellifera, respectively. A method to discriminate KNH and EH was established by using duplex polymerase chain reaction (PCR) in this study. Immunochromatographic assay (IC) was examined to analyze the duplex PCR product. The DNA sequences of primers for the duplex PCR were determined by comparing cytochrome C oxidase genes of the two honey bee species. Chelex resin method was more efficient in extracting genomic DNA from honey than the other two procedures of commercial kits. The duplex PCR amplifying DNA of 133 bp were more sensitive than that amplifying DNA of 206 bp in detecting EH in the honey mixture of KNH and EH. Agarose gel electrophoresis and IC detected the DNA of 133 bp at the ratios of down to 1% and 5% EH in the honey mixture, respectively and also revealed that several KNH products distributed by internet shopping sites were actually EH. In conclusion, the duplex PCR with subsequent IC could also discriminate between KNH and EH and save time and labor.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.9 no.1
• /
• pp.108-125
• /
• 2015

We propose a new time-division half-duplex estimate-and-forward (EF) relaying strategy suitable for a relay with mobility. We reconfigure EF relaying to guarantee a strong relay-destination link which is required to achieve a high rate using EF relaying. Based on the reconfigured model, we optimize the relaying strategy to attain a high rate irrespective of the relay position with preserving the total transmit bandwidth and energy. The proposed relaying strategy achieves high communication reliability for any relay position, which differs from conventional EF and decode-and-forward (DF) relaying schemes.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.8 no.5
• /
• pp.1076-1081
• /
• 2007

In this paper, we propose a new switch structure for time division duplex(TDD) system. The existing TDD structure utilizes a circulator fur isolation characteristic between ports. However, the circulator produces intermodulation distortion signals which are undesired signal because of its nonlinear properties. The proposed circuit is composed of a modified branch-line hybrid coupler which controls the signal flow while the isolated port is open-/short- terminated. In order to prove the validity of the presented structure, the switch circuit is fabricated and measured at 2.3GHz, the center frequency of Wibro service system.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.8
• /
• pp.1398-1403
• /
• 2016

The simultaneous detection and accurate identification of hepatitis A virus (HAV) is critical in food safety and epidemiological studies to prevent the spread of HAV outbreaks. Towards this goal, a one-step duplex reverse-transcription (RT)-PCR method was developed targeting the VP1/P2B and VP3/VP1 regions of the HAV genome for the qualitative detection of HAV. An HAV RT-qPCR standard curve was produced for the quantification of HAV RNA. The detection limit of the duplex RT-PCR method was 2.8 × 10¹ copies of HAV. The PCR products enabled HAV genotyping analysis through DNA sequencing, which can be applied for epidemiological investigations. The ability of this duplex RT-PCR method to detect HAV was evaluated with HAV-spiked samples of fresh lettuce, frozen strawberries, and oysters. The limit of detection of the one-step duplex RT-PCR for each food model was 9.4 × 10² copies/20 g fresh lettuce, 9.7 × 10³ copies/20 g frozen strawberries, and 4.1 × 10³ copies/1.5 g oysters. Use of a one-step duplex RT-PCR method has advantages such as shorter time, decreased cost, and decreased labor owing to the single amplification reaction instead of four amplifications necessary for nested RT-PCR.

• Electronics and Telecommunications Trends
• /
• v.30 no.3
• /
• pp.123-130
• /
• 2015

본고는 TDD(Time Division Duplex)/FDD(Frequency-Division Duplex) 네트워크 컨버전스를 통해 LTE(Long Term Evolution) 서비스를 제공하고 있는 주요 사업자들의 동향에 대하여 분석 정리하였다. 본고의 분석 결과에 따르면, 상당수 사업자가 보유 주파수 여건을 고려하여 경쟁력을 높이기 위한 목적으로 네트워크 컨버전스를 추진한 것으로 나타났다. 향후 국내에서도 TDD/FDD 컨버전스를 촉진하고 주파수 이용 효율성을 개선하기 위한 정책방안에 대한 모색이 필요하다고 사료된다.