• 한국어병학회지
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• 제12권2호
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• pp.107-113
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• 1999

능성어의 바이러스성 신경괴사증 바이러스(fish nodavirus)의 병원성을 능성어 및 다른 해산어류를 대상으로 인위감염법에 의해 조사한 결과, 능성어 바이러스성 신경괴사증 바이러스는 능성어에서는 소형어(27 g)부터 대형어(104 g)까지 제사를 일으켜 강한 병원성을 나타냈지만, 실험에 사용한 크기의 방어(537 g), 참돔(207 g), 돌돔(43 g), 넙치(41 g), 자주복(27 g) 및 조피볼락(94 g)에는 폐사를 일으키지 않아 병원성을 나타내지 않았다. 그리고 사육수온별 능성어에 대한 바이러스의 병원성은 수온 $15^{\circ}C$에서는 병원성을 나타내지 않았고, 수온 $20\sim28^{\circ}C$에서는 수온이 높을 수록 병원성을 강하게 나타냈다. 그리고 바이러스는 근육주사, 복강 주사 및 경구감염뿐만 아니라 침지감염과 혼합사육감염에 의해서도 쉽게 능성어에 감염이 되지만, 감염 후 생존한 능성어에 대해서는 바이러스를 재감염시켜도 폐사가 일어나지 않았다.

• 대한한의학회지
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• 제16권1호통권29호
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• pp.9-20
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• 1995

I would like to state my own opinion on arthralgia syndrome(痺病) through the literatural studies. First of all, arthralgia symdrome(痺病) must be classified into six type basically, which are migratory arthralgia(痺病(行痺)), arthritis of heat type(濕痺), arthritis due to blood stasis(瘀血痺) and deficient rheumatism(虛痺), and then could be considered to try the compound names of arthralgia syndrome. These can come from according to the rise and decline of causes in wind(風), cold(寒), damp(濕), heat(熱), blood stasis(瘀血) and qi-blood(氣血). For example, it would be possible to apply the wind-dampness rheymatism(風濕痺) of damp-heat rheumatism(濕熱痺) in terminology of arthralgia syndrome(痺病). As rheumatoid arthritis(歷節風), rheumatoid arthritis like white tiger bite (白虎歷節風) and gout (痛風) not to mean the gout in western medicine have been announced a kind of arthralgia syndromes(痺病) by many doctors since Ming dynasty(明代) and proved it to be true, it is reasonabie not to try it any longer. And tingling and deficiency of sensation(廢木 不仁) is a symptome showing the decline of muscle power including mainly the abnormal sensation of skin, it would be recommended to be classified into fliaccidity syndrome(?痺). And then the names rheumatism invoiving lendon and ligament(筋痺), rheumatism involving blood vessels(脈痺), rheumatism involving muscle(肌痺), numbness of skin (皮痺) and rheumatism involving bone(骨痺), which have been used as the classification title with the season be received bad-qi(邪氣), must be classlfied to the location appearing aymptomes. Though obstruction of the liver-qi(肝痺), obstruction of the heart-qi(心痺), stagnation of the spleen-qi(脾痺), stagnation of the lung-qi(肺痺), stagnation of the kidney-qi(腎痺) and dysfunction of the bladder(胞痺) that used visceral and bladder name, that stated a kind of arthralgia syndrome(痺病), but it must be classified into a different diseases from arthragia syndrome.

• BMB Reports
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• 제39권1호
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• pp.37-45
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• 2006

Bulked segregant analysis (BSA) and AFLP were used for isolation of genomic sex determination markers in Penaeus monodon. A total of 256 primer combinations were tested against 6-10 bulked genomic DNA of P. monodon. Five and one candidate female- and male-specific AFLP fragments were identified. Female-specific fragments were cloned and further characterized. SCAR markers derived from FE10M9520, FE10M10725.1, FE10M10725.2 and FE14M16340 provided the positive amplification product in both male and female P. monodon. Further analysis of these markers using SSCP and genome walk analysis indicated that they were not sex-linked. In addition, sex-specific (or differential) expression markers in ovaries and testes of P. monodon were analyzed by RAP-PCR (150 primer combinations). Twenty-one and fourteen RAP-PCR fragments specifically/differentially expressed in ovaries and testes of P. monodon were successfully cloned and sequenced. Expression patterns of 25 transcripts were tested against the first stranded cDNA of ovaries and testes of 3-month-old and broodstock-sized P. monodon (N = 5 and N = 7 - 10 for females and N = 4 and N = 5 - 7 for males, respectively). Five (FI-4, FI-44, FIII-4, FIII-39 and FIII-58) and two (M457-A01 and MII-51) derived RAP-PCR markers revealed female- and male-specific expression patterns in P. monodon. Surprisingly, MII-5 originally found in testes showed a higher expression level in ovaries than did testes of juvenile shrimps but a temporal female-specific pattern in P. monodon adults.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.65-65
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• 2001

본 연구는 칡소 귀세포를 공여핵으로 이용한 체세포 복제송아지 생산에 있어서 배양방법이 배발생 및 배반포 발달율에 미치는 영향과 체세포 복제란의 이식후 수태율에 미치는 영향을조사하여 복제송아지의 생산 효율을 제고하고자 실시하였다. 실험에 공시된 공여핵은 칡소 의 귀세포를 회수하여 10%FBS가 첨가된 DMEM배지에서 3-4일 동안 배양하여 monolayar Confluent 형성 후 0.25% trypsin을 처리하여 준비하였으며 공여세포는 적어도 passage가 5회 이상의 세포만을 사용하였다. 복제수정란의 생산은 18-20시간 동안 체외성숙 된 난자의 핵을 제거하고 공여핵을 주입하여 2.2kv/cm, 10$\mu\textrm{s}$의 전압으로 2회 자극함으로 융합하였으며, 융합된 난자는 5$\mu\textrm{g}$/$m\ell$ ionomycin에서 4분간, 1.9mM 6-dimethyl aminopurine에서 4시간동안 배양하여 활성화처리를 하였다. 핵이식수정란의 배양은 39$^{\circ}C$, 5%$CO_2$ incubator에서 처리구 I은 CRlaa에서 4일간 배양 후 CR2aa배지에서 배양, 처리구II는 CRlaa에 4일간 배양후 CR2aa배지에 cumulus cell과 공배양, 처리구III은 CR2aa 배지에 camulus cell과 함께 배양하였다. 수정란이식은 발정발현 7일째에 비외과적 방법으로 젖소 미경산우에 이식하였으며 이식란수는 2~4개의 핵이식된 수정란을 이식하였다. 임신진단은 45~60일 사이에 직장검사 및 초음파 진단기를 이용하여 실시하였다. 배양방법에 따른 배발생율은 처리구 I에서 92.2 %(83/90)으로 처리구II와 III의 62.4%(63/101)와 77.8%(144/185)에 비하여 높게 나타났으나 배반포 발달율은 처리구II와III에서 65.1%(41/63)와 50.0%(72/144)로 처리구 I의 30.1%(25/83)보다 높게 나타났다. 각 처리구에 따른 수정란 이식후 수태율은 처리구II와 III에서 공히 20%의 수태율을 나타낸 반면 처리구 I에서는 수태가 되지 않았다. 따라서 체세포 복제수정란의 생산에 있어서 배반포 발달율과 수태율을 높이기 위해서는 단순배양보다 공배양이 더 효과적인 것으로 사료되지만 이런 결과가 복제송아지 생산효율에 있어서도 효과적일지는 향후 더 많은 연구가 있어야 할 것으로 사료된다.

• 한국수산과학회지
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• 제46권6호
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• pp.725-732
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• 2013

To identify the food component characteristics of seven edible pufferfishes (five wild pufferfishes including striped puffer Takifugu xanthopterus; eyespot puffer Takifugu chinensis; purple puffer Takifugu porphyreus; rough-backed puffer Lagocephalus wheeleri; and grass puffer Takifugu niphobles; and two cultured pufferfishes including yellow puffer Takifugu obscurus, and tiger puffer Takifugu rubripes) in Korea, the proximate, fatty/amino acid, chemical and taste compositions were investigated. The proximate compositions were not significantly different among the sampled pufferfishes, whereas grass puffer had lower moisture and crude lipid levels contents, and higher crude protein and ash contents than the other species. The total amino acid levels in wild and cultured pufferfishes were 14,941.6-16,427.9 mg/100 g, and the major amino acids were aspartic acid, glutamic acid, glycine, alanine, leucine, lysine and arginine. Regarding the fatty acid and mineral compositions, the major fatty acids included 22:6n-3, 16:0, 18:1n-9, 20:5n-3, 18:0, 20:4n-3, and 22:5n-3 in that order, and there was a little difference among the sampled pufferfishes. Grass puffer had a higher monoenes ratio, i.e., 16:1n-7 and 18:1n-9, and a lower polyenes ratio than the other pufferfishes. The main minerals detected K, P, Na, S and Ca. Regarding the taste-active compounds, the free amino acid contents of pufferfish extracts were 236.4-428.1 mg/100 g, consisting primarily of taurine, lysine, proline, glycine, alanine and arginine in that order. The amino nitrogen content of the pufferfishes was 84.5-156.4 mg/100 g, and there was a little difference among the species. As for taste intensity, the total taste value of the purple puffer was higher than that of the other pufferfishes.

• 한국양식학회지
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• 제11권2호
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• pp.261-269
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• 1998

본 연구는 부착성 요각류인 Tigriopus japonicus의 대량배양을 위해서 배양방법과 먹이효율을 조사하였다. 유수식 배양에 있어서 암컷의 생존율과 유생생산은 정수식보다 높게 나타났지만 유생의 생존율은 정수식에서 높게 나타났다. T. japonicus의 배양에 있어서 표면적이 증가할수록 T. japonicus의 증식은 효과적이였다. 또한 T. japonicus와 rotifer의 혼합배양에 있어서 rotifer는 T. japonicus의 영향을 받지 않으나 T.japonicus는 rotifer의영향을 받는 것으로 나타났다. 해산 자어에 대한 T. japonicus의 먹이효율은 참돔과 자주복 등과 같은 쪼아먹는 식성의 치어에 있어서는 Artemia nauplius보다 높은 먹이효율을 보였다. 그러나 넙치치어와 같이 저서성치어에게는 먹이로서 부적합하였다.

• 한국주거학회논문집
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• 제6권2호
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• pp.181-189
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• 1995

It is thought as more important for us to understand foreign countrie's culture in the modern society. because the world is becoming closer day by day thiough the develepment of communication and mass-media. Cross-cultural study could play a role to give an opportunity for people to understand foreign countrie’s culture easily.In this respect, this study was planned to find out similarity and difference between Danish and Korean folk tables made and used before the 20th century during the 18th-l9th century as a part of the comparative study of folk furniture between the two countries. It would be very interesting if we could find something valuable between Danish and Korean folk furniture. because they are located very far away each other geographically with less cultural communication before.There were more differences than similarity between the folk tables of the two countries and major findings were as followings : Firstly, there were many kinds of space-saving tables in Denmark. whereas only a few kinds of tables which could be removed easily after their usage were found in KoreaSecondly, Banish folk tables were more emphasized on the function rather than decoration. Whereas Korean folk tables were smaller lower and more decorative than Danish ones to fit their life-style of sitting on the floor.Thirdly, many Danish folk tables were made of bare wood. while Korean ones were finished by transparent lacquer or vegetable oil. Stone tops if tables were rare in both countries. Lastly. there were common factors in the design of legs of folk tables in both countries. It was interesting that animal legs such as deg tiger cat. lion, and eagle were used for design motif of the table legs. But trestle leg, cross leg and gate leg were not found in Korean folk tables. As a conclusion, throughout this study, it became obvious that life-style was one of the most important factor influencing on the design of furniture.

• 천문학논총
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• 제23권1호
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• pp.1-12
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• 2008

Understanding of the basic characteristics of an astronomical instrument is a prerequisite to obtaining reliable data from the instrument. We have analyzed more than 1,000 calibration images from the Fairchild 486 CCD (hereafter the Maidanak 4k CCD system) attached to the AZT-22 1.5m telescope at Maidanak Astronomical Observatory in Uzbekistan. The Maidanak 4k CCD system supports three readout modes through 1, 2, or 4 amplifiers. In most cases observers use 4-amplifier readout mode to save time. We have tested the stability and seasonal variation of zero levels and confirm that two quadrants of the images (Amp 1 & 2) show no appreciable seasonal variation. but the other two quadrants (Amp 3 & Amp 4) show an evident seasonal variation in the bias level. The Cryo Tiger, the cooling system used at the Maidanak 4k CCD system, maintains the CCD temperature at -108'E, and effectively suppresses the dark electrons. The mean value versus the variance plot of the flat images does not show the expected relation for an ideal Poisson noise distribution and this is attributed to the large variation in quantum efficiency between different pixels. In addition, we confirm that there is no appreciable difference in gain between readout amplifiers, but there is a large variation in quantum efficiency across CCD chip especially in U. Due to the finite length of shutter opening and closing time, the effective exposure time varies across the science images. We introduce two parameters to quantify the effect of this uneven illumination and present a method to remove these effects. We also present a method to remove the interference patterns appearing in the images obtained with longer wavelength filters and investigate the spatial variation of the point spread function.

• Parasites, Hosts and Diseases
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• 제49권4호
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• pp.445-448
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• 2011

A survey was performed to find out the intermediate hosts of Gnathostoma nipponicum in Jeju-do (Province), the Republic of Korea. In August 2009 and 2010, a total of 82 tadpoles, 23 black-spotted pond frogs (Rana nigromaculata), 7 tiger keelback snakes (Rhabdophis tigrinus tigrinus), 6 red-tongue viper snakes (Agkistrodon ussuriensis), and 2 cat snakes (Elaphe dione) were collected in Jeju-do and examined by the pepsin-HCl digestion method. Total 5 gnathostome larvae were detected in 3 (50%) of 6 A. ussuriensis, 70 larvae in 3 of 7 (42.9%) R. tigrinus tigrinus, and 2 larvae in 2 of 82 (8.7%) frogs. No gnathostome larvae were detected in tadpoles and cat snakes. The larvae detected were a single species, and $2.17{\times}0.22mm$ in average size. They had characteristic head bulbs, muscular esophagus, and 4 cervical sacs. Three rows of hooklets were arranged in the head bulbs, and the number of hooklets in each row was 29, 33, and 36 posteriorly. All these characters were consistent with the advanced third-stage larvae of G. nipponicum. It has been first confirmed in Jeju-do that R. nigromaculata, A. ussuriensis, and R. tigrinus tigrinus play a role for intermediate and/or paratenic hosts for G. nipponicum.

• 한국식품조리과학회지
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• 제27권1호
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• pp.85-99
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• 2011

Grinding is an indispensible step in preparation of espresso coffee. The purpose of this study was to evaluate physicochemical and sensory characteristics of espresso according to the grinding grades of coffee beans. Specifically, physicochemical characteristics according to the grinding grades of coffee beans were tested based on foam index, persistence of foam, total solids, concentration, extraction yield, pH, viscosity, total soluble solid content and density. With regard to sensory characteristics, QDA were carried out by 10 trained panels. Persistence of foam, total solids, concentration, extraction, pH and viscosity in physicochemical test showed significant differences among the three categories of the samples. In other words, the finer the size of particles was, the higher value was, at the p=0.001 value. Meanwhile, the value of foam index and total soluble solid content to the grinding grades was the highest in 'fine', at the p=0.001 value. Tiger-skin effect in crema, an important attribute for excellent espresso coffee, was perceived by all of the panels in 'fine'. Also, intensity of flavor was perceived as to the strongest in 'fine'. Moreover, 'fine' had the sweetness to balance the acids and bitters. In conclusion, according to the grinding grades, it was identified that there were differences in the physicochemical and sensory characteristics. Furthermore, when the size of particles was 'fine', it brought to the most positive result.