• ALGAE
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• v.25 no.1
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• pp.45-56
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• 2010

In this study, we assessed the antioxidant properties of tidal pool microalgae, Halochlorococcum porphyrae and Oltamannsiellopsis unicellularis, from Jeju Island, Korea. Specifically, the antioxidant activity of fractions isolated from 80% methanol extract, and digests produced from five proteases and carbohydrases, were investigated. Almost all the fractions and the 80% methanol extract exhibited higher effects on 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging. The ethyl acetate fraction showed the highest superoxide anion scavenging activity, while both n-hexane and chloroform fractions exhibited higher $H_2O_2$ scavenging activity. Among the enzymatic digests from H. porphyrae and O. unicellularis, all the digests exhibited remarkable DPPH scavenging activities. In nitric oxide inhibition, all the digests recorded significantly higher effects than those of the commercial antioxidants (p < 0.05). Flavozyme and Neutrase digests from H. porphyrae, and Termamyl and Alcalase digests from O. unicellularis, showed significant effects in metal chelating. Lipid peroxidation was significantly inhibited in the ethyl acetate fraction, in the Celluclast and Protamex digests from H. porphyrae, and in the chloroform fraction from O. unicellularis. These findings suggest that the two tidal pool microalgae tested in this study are rich in potential antioxidative compounds, the specific properties of which can be considered for use in the food and pharmaceutical industries.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.184-192
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• 2006

To investigate reproductive cycle of Tridentiger obscurus in Jeju, total 408 fish were collected in brackish area (May 2004 to August 2005) and total 271 fish were collected in tidal pool (July 2004 to August 2005), respectively. Water temperature ranged $11.0{\sim}21.6^{\circ}C$ in brackish area. Water temperature of tidal pool ($11.1{\sim}24.5^{\circ}C$) in 2005 was similar to that of brackish area although it were highest $30.1{\sim}29.2^{\circ}C$ from July to August in 2004. In brackish area, female gonadosomatic index (GSI) increased in April and maintained high values from May to August. The reproductive cycle can be grouped into the following successive stages in the ovary: immature (November to May), maturing (March to September), ripe and spawning (May to September), and degenerating (July to December). In the tidal pool, female GSI rapidly increased in April and maintained high values until July in 2005. However, it has been low in July 2004 when water temperature was highest. The reproductive cycle can be grouped into the following successive stages in the ovary: immature (August to April), maturing (March to August), ripe and spawning (March to July), and degenerating (July to August 2004 and August 2005). Fecundity of mature female ranged from 1,214 to 12,109 in brackish and ranged from 2,427 to 13,892 in tidal pool area. The fecundity of fish in the tidal pool was significantly higher than that of fish in brackish area. Fecundity was correlated positively with total length and body weigh. Although the large group (> 111 mm total length) in brackish area observed only in spawning season, one in the tidal pool observed from October 2004 until next spawning season. Frequency of the large group was 0 to 12% and 11.4 to 57.9% in brackish area and tidal pool, respectively. These results were indicated that gonadal development of T. obscurus was affected to water temperature and day length.

• Fisheries and Aquatic Sciences
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• v.23 no.7
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• pp.17.1-17.4
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• 2020

Six specimens of Bathygobius hongkongensis were collected for the first time from the eastern coast of Jeju Island, Korea, in September-November 2017. This species is characterized by a pectoral fin with free rays and the division of the first dorsalmost pectoral free ray into three branches; a lower jaw with a mental frenum; and an anterior nostril with a tiny flap. Bathygobius hongkongensis is similar to B. fuscus but differs in having more dorsalmost free pectoral fin rays. The new Korean name proposed for B. hongkongensis is "Nam-bang-mu-nui-mang-duk."

• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.47-51
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• 2016

Three specimens of Abudefduf septemfasciatus were collected firstly from tidal pools on the southern and northern coasts of Jeju Island, Korea in August 2015. This species is characterized by black blotches on the upper origin of the pectoral fin, upper caudal peduncle and a dorsal fin between the second and fourth spines, predorsal scales beyond to above the middle of the eye, 12~13 soft dorsal and anal fin rays, and 20 lateral-line scales. This species is distinguishable from Abudefduf sordidus by soft dorsal and anal fin rays, location of caudal peduncle blotch, and location of the dorsal fin blotch. The newly proposed Korean name is "Il-gop-jul-ja-dom".

• Environmental Analysis Health and Toxicology
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• v.23 no.2
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• pp.93-100
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• 2008

The ecotoxicological effects of 4-tert-octylphenol were observed on Harpacticoid copepoda Tigriopus japonicus s.l. gathered and cultured from tidal pool of Korean coast. There were no significant differences in survival rate (except 10 $\mu$g/L; 70.00%) and sex ratio (except 30 $\mu$g/L) on T. japonicus s.l. exposed to 4-tert-octylphenol. However, 4-tert-octylphenol induced developmental delay (copepodite emergence day and adult male mergence day) and retardation of first brooding day of adult female. Moreover the body size and biomass decreased at 4-tert-octylphenol exposure. As a result, detailed life-cycle research of T. japonicus s.l. may yield potential bioindicators for environmental monitoring and assessment.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.4
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• pp.310-321
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• 2016

This study describes the spatial distribution of dinoflagellate cyst assemblages from the fishing ports along Jeju Island. Surface sediment samples from 22 stations revealed the occurrence of 29 species involving the Groups Protoperidinioid (44.8%), Gonyaulacoid (31.0%), Calciodineloid (10.3%), Gymnodinioid (6.9%), Diplosalid (3.5%) and Tuberculodinioid (3.5%). The cyst abundance recorded here is very low ($13{\sim}220cysts\;g-dry^{-1}$) as compared to Korean coastal regions. The abundance of heterothophic cysts increased in several fishing pots with fine sediments and anthropogenic tidal pools. And cyst abundance was well correlated with the grain-size composition of surface sediments. The dinoflagellate cyst assemblages in Jeju fishing ports were characterized by the dominant species, cyst of Gymnodinium sp., cyst of Pyrodinium bahamense and cyst of Scrippsiella trochoidea in 2012, Protoperidinium sp. (Brigantedinium sp.), cyst of Scrippsiella sp./trochoidea and cyst of Gymnodinium sp. in 2014, and Protoperidinium sp. (Echinidinium sp. and Brigantedinium sp.) in 2016. The advent of the toxic dinoflagellate, Pyrodinium bahamense were recorded for the first time in Jeju coastal waters. As a results, we are determined should be to monitoring and management measures for new toxic dinoflegallates from tropical or subtropical reigions and anthropogenic tidal pools by industrial activities.

• Animal Systematics, Evolution and Diversity
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• v.34 no.3
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• pp.143-151
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• 2018

A marine ciliate Amphisiella milnei (Kahl, 1932) $Horv{\acute{a}}th$, 1950 was discovered from the tidal pool of Baekdo Island, South Korea. The existence of extra cirri between leftmost frontal cirrus and buccal cirrus discriminates this species from its congeners. Its morphological features are described as follows: body size in vivo $110-130{\times}35-45{\mu}m$; elongate rectangular to elliptical in shape; two large and several small ring-shaped structures; yellowish cortical granules arranged irregularly on ventral side but longitudinally along dorsal kineties on dorsal side; 34-40 adoral membranelles, 3 frontal cirri, 1 buccal cirrus, 1 parabuccal cirrus, usually 2 extra cirri behind leftmost frontal cirrus, and 3 frontoventral cirri; amphisiellid median cirral row composed of 25-31 cirri with 27-36 left and 27-44 right marginal cirri; usually 5 transverse cirri and 2 pretransverse cirri with 7 dorsal kineties; two macronuclear nodules. In addition to, 18S rDNA sequence of A. milnei was analyzed to understand its phylogenetic relationship.

• Korean Journal of Ichthyology
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• v.17 no.2
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• pp.142-147
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• 2005

To research changes in the fish community of intertidal zones in relation to the construction of the Saemangeum tidal embankment, fishes were collected in the tide pools of Naechodo, Gunsan-si, and of Haechang, Buan-gun in Jeollabuk-do, every month from December 2002 to October 2004. Based on the fishes collected and data from previous studies show that in the tide pools of Haechang, the number of species decreased, and the dominant species is Tridentiger trigonocephalus, as before. However, in the tide pools of Naechodo, the number of species decreased, and the dominant species changed from T. trigonocephalus to Synechogobius hasta. Favonigobius gymnauchen, which inhabits sand-beds, decreased remarkably. Meanwhile, in the intertidal zone of Haechang, young black porgy were caught in large quantities, while in the intertidal zone of Naechodo, young Acanthopagrus schlegelii and Lateolabrax japonicus, which had been caught in a large amounts between 1994 and 2000, were not caught. The results are thought to show that the intertidal zone of Naechodo, Gunsan-si, had more deposits by tide flats than that of Haechang, Buan-gun, which drastically changed the habitat of its fish community.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.92-92
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• 2003

본 연구는 부착규조류에 따른 부착성 요각류 Tigriopus japonicus의 nauplius 생산력을 알아보기 위하여 실시하였다. 실험에 사용된 T. japonicus는 부산 동백섬 부경대학교 수산과학연구소 부근에 있는 tidal pool에서 동물성부유생물망으로 채집하였다. 먹이생물로는 부경대학교 한국해양 미세조류 은행에서 보관중인 부착규조류 중 중형종인 Caloneis schroder를 대표종으로 이와 크기가(14.8~27.5$\mu\textrm{m}$) 유사한 종들을 대상으로 형태를 현미경 하에서 관찰하고, 지역과 분리일자 등을 고려하여 유사종을 선별한 후 최종적으로 종의 유전적 유사성을 밝히기 위하여 RAPD-PCR (random amplified polymorphic DNA polymerase chain reaction)을 실시하였다. 이 중 Caloneis schroder와 유전적으로 유사성이 낮은 Navicula spp. (KMCC B-245, 393, 394, 581) 4종을 선별하여 T. japonicus의 먹이로 사용하여 포란한 암컷의 nauplius 생산력을 3반복 조사하였다. Genomic DNA는 대부분의 종에서 성공적으로 검출되었으며, 종에 따라 PCR 증폭산물이 나타나지 않은 경우도 있었으므로, PCR 산물이 나타난 종에 대해서만 분석하였고, 증폭된 DNA band는 대부분 크기 0.5~2.0kb 범위에서 나타났다. 실험에 사용된 부착규조류 간의 유사성을 알아보기 위하여 similarity matrix를 분석한 결과 F값의 범위는 0.00에서 1.00까지 였으며, Caloneis schroder와 유사성이 낮은 종들에 비하여 유사성이 높은 종들이 더 많이 나타났다. 이들을 먹이로 하여 포란한 T. japonicus의 실험구별 nauplius 평균 개체수를 살펴보면, KMCC B-394가 255.7마리로 가장 높았던 반면 KMCC B-581가 29.7마리로 가장 낮았다. 그 외 KMCC B-245가 120.0마리, KMCC B-393가 76.0마리, Caloneis schroder가 32.3마리 각각 나타났다. 이와같은 결과를 볼 때 T. japonicus의 nauplius 생산력은 규조 종에 따라 큰 차이가 있는 것으로 판단된다.

• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.28-34
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• 2016

The anatomy and histology of the olfactory organ in Favonigobius gymnauchen was investigated using a stereo microscopy, light microscopy and scanning electron microscopy. The paired olfactory organs in the dorsal snout are set in between the upper lip and the eyes. These organs are composed of two openings (anterior nostril with a tubular structure and posterior nostril), a single olfactory cavity, two nasal sac (ethmoidal and lacrimal sacs), olfactory nerve and olfactory bulb. The distributional pattern of the sensory epithelium is a only one type (continuous type). This epithelium is made up of the receptor cell, supporting cell and basal cell. The receptor cell has a only one type (ciliated receptor cell with 3~4 cilia). The non-sensory epithelium is built of the stratified epithelial cells and has mucous openings on the surface. Such an olfactory organ in F. gymnauchen may be considered to reflect its ecological habitat as a shallow water or tidal pool in the coastal zone.