• 제목/요약/키워드: thymocytes

검색결과 154건 처리시간 0.036초

계기복령환이 수종(數種)의 암세포주(癌細胞柱) 및 면역기능(免疫機能)에 미치는 영향(影響) (The effect of KaegiBokryengHwan on sereval cancer cell lines and immuno-function)

  • 강성도;진천식;정현우
    • 대한한의학방제학회지
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    • 제7권1호
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    • pp.107-120
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    • 1999
  • The purpose of this Study was to investigate effects of KaegiBokryengHwan(KBH) on anti-tumor, immunocytes and nitric oxide(NO). This Study estimated the proliferation of L1210 cell lines, HeLa cell lines, SK-OV3 cell lines, MCF-7 cell lines, balb/c mouse 3T3 cell lines, mouse thymocytes and mouse splenocytes and NO production from peritoneal macrophages in vitro. and estimated the proliferation of L1210 cells, mouse thymocytes and splenocytes and NO production from peritoneal macrophages and body weight in L1210 cells-transplanted mice in vivo. The result were obtained as follow ; 1. KBH inhibited significantly SK-OV3 cell lines in vitro. 2. KBH was accelerate significantly the proliferation of balb/c mouse thymocytes in vitro. 3. KBH increased significantly NO production from peritoneal macrophages in vitro. 4. KBH didn't effect the cytotoxicity of L1210 cells in L1210 cells-transplanted mice. 5. KBH was accelerate the proliferation of splenocytes in L1210 cells-transplanted mice. 6. KBH increased NO production from peritoneal macrophages in L1210 cells-transplanted mice. 7. KBH increased the body weight as comparing with control group in L1210 cells-transplanted mice.

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托裏黃기湯이 抗癌 및 免疫作用에 미치는 實驗的 效果 (Experimental Effects of Taklihwangki-Tang on the Anti-Cancer And Immuno-Action)

  • 정동환;최정화;김종한;정현우
    • 한방안이비인후피부과학회지
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    • 제15권2호
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    • pp.118-130
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    • 2002
  • Taklihwangki-Tang was a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effect of Taklihwangki-Tang on the anti-cancer and proliferation of immunocytes, nitric oxide(NO) production of peritoneal macrophages. We used Taklihwangki-Tang extract(THT) with freeze-dried, 8wks-old male mice and cancer cell lines(L1210, S-180) for this Study. The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follow ; THT was showed cytotoxicity on the L1210 and S-180 cell lines, increased proliferation of thymocytes. And the combined effects of THT and vincristine were became cytotoxicity of cancer cell lines and increased significantly proliferation of thymocytes. THT accelerated proliferation of thymocytes in normal mice, and decreased significantly proliferation of L1210 cells and accelerated significantly NO production of peritoneal macrophages in L1210 cells transplanted mice. This results suggest that THT inhibit proliferation of cancer cells by becoming immunocytes activity(NO production, proliferation of T-cell).

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Effect of Kamiinsamyangyoung-tang on Immune Response and Blood Formation

  • Park, Kyung-Mi
    • 동의생리병리학회지
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    • 제19권1호
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    • pp.262-269
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    • 2005
  • Kamiinsamyangyoung-tang(KIT) has been widely used to treat amenorrhea and bradymenorrea caused from vital energy and blood deficit. KIT was composed of Insamyangyoung-tang, Cervi Cornu Pantotrichum and Hominis Placenta. The aim of this study is to investigate effect of KIT on Immune response and Blood formation. We investigated thymocytes, splenocytes population, ${\gamma}$-interferon, IL-2, IL-4, NO and phagocytic activity. We found that KIT had no effect on the Th and Tc cell population of thymocytes, Th cell population of splenocytes and ${\gamma}$-interferon quantity was decreased. KIT decreased the formation of Nitric Oxide from abdominal macrophage, on the other hand, it had no influence on the quantity of IL-2, IL-4.

Genistein이 T lymphocyte의 Apoptosis 및 복강 Macrophage의 탐식능에 미치는 영향 (Effect of Genistein on Apoptosis of T Iymphocyte and Phagocytosis of Peritoneal Macrophage)

  • 은재순;조선경;이택렬;김대근;오찬호;소준노
    • 약학회지
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    • 제46권1호
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    • pp.69-74
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    • 2002
  • The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vitro. Addition of genistein (10 and 50$\mu$M) to cultured thymocytes from BALB/c mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in mitochondrial transmembrane potential ($\Delta$Ψm). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in $\Delta$Ψm and decrease phagocytic activity of peritoneal macrophage in vitro.

홍화(紅花)가 인체(人體)의 암세포주(癌細胞柱)에 미치는 영향(影響) (Cytotoxicity of Carthami Flos on Human cancer cell-lines(I))

  • 한종현;유광석;강성용
    • 대한한의학회지
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    • 제17권2호
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    • pp.303-310
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    • 1996
  • The purpose of this study was to investigate effect of water extract of Carthami Flos on the proliferation of human cancer cell-lines. The effects of Carthami Flos on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. The results were as follows; 1. Carthami Flos did not effect A431, HeLa, MOLT-4, K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was increased by the combination of Carthami Flos. 3. Carthami Flos inhibited the proliferation of Balb/c 3T3 cells. 4. Carthami Flos stimulated the proliferation of thymocytes. 5. Carthami Flos stimulated the proliferation of splenocytes. 6. Carthami Flos stimulated the proliferation of human lymphocytes.

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가미보아탕(加味補兒湯)의 면역조절작용(免疫調節作用)에 대(對)한 실험적(實驗的) 연구(硏究) (Experimental Study on the Immunoregulative Action of Kamiboatang)

  • 정연희;유동열
    • 대한한방소아과학회지
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    • 제14권2호
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    • pp.61-84
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    • 2000
  • The purpose of this study was to investigate the effects of Kami boatang(KBT) on the immune cells in Balb/c mice. KBT (500mg/kg) was administerd p.o. once a day for 7 days. KBT enhanced the proliferation of thymocytes and splenocytes. KBT enhanced the subpopulation of helper T(Th) cells, but did not affect the subpopulation of Thyl/B220 cells and Th/Tc cells in splenocytes. KBT enhanced the production of ${\gamma}$-interferon and interleukin-2 in thymocytes, but decreased the production of interleukin-4. KBT enhanced the production of ${\gamma}$-interferon, interleukin-2 and interleukin-4 in splenocytes and serum. KBT suppressed the production of nitric oxide, and enhanced the phagocytic activity in peritoneal macrophages. These results suggest that KBT has a potent activity on the immune response via the increase of the production of cytokines and phagocytic activity in vivo.

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當歸飮子加蟾수가 皮膚癌細胞(A431)의 細胞毒性에 미치는 影響 (Cytotoxicity of water extract of Dangkwieumja ka Sumsoo on A43l Cells)

  • 최정화
    • 한방안이비인후피부과학회지
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    • 제9권1호
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    • pp.1-15
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    • 1996
  • The purpose of this research was to investigate effect of water extract of DangKwi-Eum-Ja ka Sumsoo(DESE) on the cytotoxicity of human epidemloid cell, A431 cells. The effects of DESE on the proliferation of A431 cells, Balb/c 3T3 cells, mouse thymocytes and splenocnes were estimated by MTT colorimetric assay, and nitric oxide production from mouse peritoneal macrophage was estimated by Griess method. DESE inhibited the proliferation of A431 cells at $10{\mu}g/ml$, and did not affect the proliferation of Balb/c 3T3 cells. DESE decreased the cytotoxicity of mitomycin C or cisplatin on A431 cells, increased the cytotoxicity of mitomycin C or cisplatin on Balb/c 3T3 cells. DESE inhibited the proliferation of mouse thymocytes and splenocytes at $100{\mu}g/ml$. DESE did not affect the nitric oxide production from mouse peritoneal macrophage in vitro, but decreased the nitric oxide production from DESE-treated mouse peritoneal macrophage.

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Mouse Thymocyte Cytolysis of Several Anti-inflammatory Steroid Derivatives

  • Lee, Seon-Hyang;Choi, Hong-Pil;Namgoong, Soon-Young;Kim, Kyeong-Ho;Kim, Hyun-Pyo
    • Archives of Pharmacal Research
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    • 제13권2호
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    • pp.161-165
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    • 1990
  • For evaluating the cytolytic effects on the mouse thymocytes, four typical antiinflammatory steroids (dexamethasone, triamcinolone acetonide, prednisolone, hydrocortisone) were selected in this study. When steroids were treated to the mouse thymocytes in vitro cytolysis occurred with dose-dependent fashion and the activities were found to be paralle with the known local anti-inflammatory activities. In vivo thymus atrophogenic activities appeared by the treatment of topical and subcutaneous applications of the derivatives were also found to dose-dependent, but not coincided with the thymocyte cytolytic activities in vitro and local anti-inflammaatory activity in the case of triamcinolone acetonide. Triamicinolone acetonide induced potent thymocyte cytolysis in vitro, but showed less thymus atrophy.

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급각자가 암세포(癌細胞)에 미치는 영향(影響)

  • 한종현
    • 혜화의학회지
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    • 제5권2호
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    • pp.499-499
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    • 1997
  • The purpose of this study was to investigate the effect of Gleditsiae Spina on the pro life-ration of human cancer cell-lines. The effects of Gleditsiae Spina on the proliferation of A431, HeLa. MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. The results were as follows; 1. Gleditsiae Spina increased the proliferation of HeLa, MOLT-4 and K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was increased by the combination of Gleditsiae Spina. 3. Gleditsiae Spina did not effect the proliferation of Balb/c 3T3 cells. 4. Gleditsiae Spina stimulated the proliferation of thymocytes. 5. Gleditsiae Spina stimulated the proliferation of splenocytes. 6. Gleditsiae Spina stimulated the proliferation of human lymphocytes.

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가미보중익기탕(加味補中益氣湯)이 생쥐의 면역반응(免疫反應)에 미치는 영향(影響) (Effect of Kamibojoongikkitang on Immune Response in C57BL/6 Mice)

  • 송종석;신선미;김수민;김의일;이정은;유동열
    • 대한한방부인과학회지
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    • 제19권4호
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    • pp.1-16
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    • 2006
  • Purpose : The purpose of this research was to investigate the effects of Kamibojoongikkitang (KBT) on the immune cells in C57BL/6 mice. Methods : KBT (500mg/kg) was administerd p.o. once a day for 7 days. Results : KBT decreased the cell viability of thymocytes in vivo and in vitro system and decreased the cell viability of splenocytes in vivo, but increased the viability of splenocytes in vitro system. In addition, KBT did not affect the population of helper T (Th) cells and cytotoxic T (Tc) cells in thymocytes and decreased the population of T- and B-lymphocytes and the population of Th and Tc cells in splenocytes. Furthermore, KBT did not affect the production of ${\gamma}%-interferon and interleukin-4 in splenocytes. KBT increased the production of nitric oxide in vivo but decreased the production of nitric oxide in vitro system. KBT enhanced the phagocytic activity of peritoneal macrophages in vivo, but decreased the phagocytic activity in vitro. Conclusion : KBT has an inhibitory action on the specific immune response via decrease of the cell viability of thymocytes and splenocytes and has a potent action on the non-specific immunity via increase of phagocytic activity of peritoneal macrophages.

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