Effect of Kamiinsamyangyoung-tang on Immune Response and Blood Formation

  • Park, Kyung-Mi (Department of Gynecology, College of Oriental Medicine, Dongshin University)
  • Published : 2005.02.25

Abstract

Kamiinsamyangyoung-tang(KIT) has been widely used to treat amenorrhea and bradymenorrea caused from vital energy and blood deficit. KIT was composed of Insamyangyoung-tang, Cervi Cornu Pantotrichum and Hominis Placenta. The aim of this study is to investigate effect of KIT on Immune response and Blood formation. We investigated thymocytes, splenocytes population, ${\gamma}$-interferon, IL-2, IL-4, NO and phagocytic activity. We found that KIT had no effect on the Th and Tc cell population of thymocytes, Th cell population of splenocytes and ${\gamma}$-interferon quantity was decreased. KIT decreased the formation of Nitric Oxide from abdominal macrophage, on the other hand, it had no influence on the quantity of IL-2, IL-4.

Keywords

References

  1. 陳師文 等 : 太平惠民和劑局方, 族風出版社印行, pp.165-166, 1990.
  2. Oriental Gynecology textbook compilation committee : Oriental Gynecology(上), Seoul, JungDam, p.121, 141, 2001.
  3. Oriental college of Daejeon University : Gynecology Prescription, Daejeon, Wood and soil(도서출판 木과 土), p.55, 2000.
  4. Wysocki, L.J. and Sato, V.L. : Planning for lymphocytes: A method for cell selection. Proc. Natl. Acad. Sci. USA. 75, 2844, 1978.
  5. Mizel, S.B., Openheim, J.J. and Rosensteich, D.L. : Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. J. Immunol. 120, 1497, 1979.
  6. Mosmann, T. : Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. methods. 65, 55-63, 1983.
  7. Kotnic, V. and Fleischmann, W.R.Jr. : A simple and rapid method to determine hematopoietic growth factor activity. J. Immunol. methods. 129, 23, 1990.
  8. Suda, T. and Nagata, S. : Purification and characterization of the Pas-ligand that induces apoptosis. J. Exp. Med., 179, 873-879, 1994.
  9. Rockett, K.A., Awburn, M.M., Cowden, W.B. and Clark, I.A. : Killing of Plasmodium faciparum in vitro by nitric oxide derivatives. Infec. Immunity, 59(9), 3280, 1991.
  10. Boudard, F., Vallot, N., Cabaner, C. and Bastide, M. : Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. J. Immunol. Methods, 174, 259, 1994.
  11. Blair, A.L., Cree, I.A., Beck, J.S. and Hating, M.J.G. : Measurement of phagocyte chemiluminenscence in a microtiter plate format. J. Immunol. Methods, 112, 163, 1988.
  12. Chok, P.W., Choon, S.P. and Benjamin, H.S. : A rapid and simple microfluorometric phagocytosis assay. J. Immuno. Methods, 162, 1, 1993.
  13. Cho, B.S., Rho, Y.5., Hong, N.D. and Shin, K.H. : Studies on the anti-enemic ation of Melanterite. Kor. J. Pharmacogn. 19(2), 111-119, 1988.
  14. Hong, N.D. Lee, K.S., Whang, W.W. and Kim, N.J. : Studies on the efficacy of combined preparation of crude drug (XIX). Kor. J. Pharmacogn. 15(4), 188-193, 1984.01, 103, 107, 1993.
  15. Son Sung Yeon editing(孫星衍 輯) : 神農本草經, 臺北, 文光圖書有限公司, pp.222-223, 1979.
  16. Shin Kil Koo : 申氏本草學, Seoul, SooMunSa, pp.41-43, 1987.
  17. Lee Cheon : 醫學入門(本草燥類), Seoul, Koryo Medical, p.204, 1989.
  18. Breiheim, G., Stendahl, O. and Dahlgren, C. : Intra- and extracellular evevts in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Infect. Immun. 45, 1, 1984.
  19. Kilbourn, R.G. and Griffith, O.W. : Overproduction of nitric oxide in cytokine-mediated and septic shock. J. Natl. Cancer Instit., 84(11), 828-831, 1992.
  20. Okkuda, Y., Sakada, S., Shimaoka, M. and Yanagihara. T. : Nitric oxide induces apoptosis in mouse splenic T lymphocytes. Immunolgy Letters, 52, 135, 1996.
  21. Albina, J.E., Abate, J.A. and Henry, W.L. : Nitric oxide production is required for murine resident peritoneal macrophages to suppress mitogen-stimulated T cell proliferation. J. Immunol., 147(1), 144-148, 1991.
  22. Channon, J. Y., Leslie, C. C. and Johnston, Jr. R. B. : Zymosan-stimulated production of phosphatidic acid by macrophages: relationship to release of superoxide anion and inhibition by agents that increase intracellular cyclic AMP. J. Leucocyte Biol. 41, 450-455, 1987.
  23. Jun, C.D., Park, S.K., Kim, J.M., Kim, J.D. and Kim, S.H. : Nitric oxide inhibits macrophage pseudopodia formation in the activated macrophages. Kor. J. Immunol. 18, 635-644, 1996.
  24. Kwon Hun Young etc. : Hematology, Seoul, Koryo Medical, p.79, p.206, 1993.
  25. Curtis, D.K., Mary, O.A. and John, D. : Toxicology, 3rd., Macmillan Pub. Co., p.112, 1986.
  26. Bauer, J.D. : Clinical Laboratory Method, Mosby, p.201, 1974.