• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.4
• /
• pp.729-733
• /
• 2002

The purpose of this research was to investigate the effects of Insamyangyoung-tang water extract (IYT) on the specific immune response in BALB/c mice. When IYT (500mg/kg) was administerd p.o. once a day for 7 days to BALB/c mice, the cell viability of thymocytes and splenocytes was increased. Also, IYT increased the population of CD4/sup +/ cells in thymocytes and the population of Thy1/sup +/ cells and CD4/sup +/ cells in splenocytes. In addition, IYT increased the production of γ -interferon and interleukin-2 from thymocytes and the production of γ -interferon from splenocytes. These results suggest that IYT enhances the specific immune response via activation of Th1 cells in thymocytes and splenocytes.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.21 no.2
• /
• pp.479-484
• /
• 2007

The purpose of this research was to investigate effects of mixed extract of Ginseng Radix and Angelicae gigantis Radix on activity of murine splenocytes and macrophages. GAE (300 mg/kg) was administered p.o. for 7 days. GAE decreased the viability of murine splenocytes in vivo. Also, GAE enhanced the population of $Thy1^+$ cells in splenocytes and the population of splenic $CD4^+$ cells. Furthermore, GAE increased the production of ${\Upsilon}$-interferon from splenocytes. GAE enhanced the production of nitric oxide and the phagocytic activity of peritoneal macrophages. These results suggest that GAE regulates the immune response via activation of splenic Th1 cells and peritoneal macrophages.

• Journal of Life Science
• /
• v.10 no.2
• /
• pp.140-149
• /
• 2000

In order to utilize marine processing waste which would normally be discarded, cod liver protein was hydrolysed by ${\alpha}$-chymotrysin, and the hydrolysate was investigated for the new angiotensin I converting enzyme (ACE) inhibitor. Thy hydrolysate was separated into three major types, with molecular weight cut-off (MWCO) values less than 10 kDa, 5 kDa and 1 kDa of ultrafiltration membranes, respectively. ACE inhibitory peptides were isolated from the fractions passed through MWCO 1 kDa membrane, and purified by using ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, and HPLC on an ODS column. The purity was identified with capillary electrophoresis. The amino acid sequences of two peptides were Met-Ile-Pro-Pro-Tyr-Tyr (IC50=10.9 ${\mu}$M) and Gly-Leu-Arg-Asn-Gly-Ile (IC50=35.0 ${\mu}$M)

• Restorative Dentistry and Endodontics
• /
• v.5 no.1
• /
• pp.35-40
• /
• 1979

The author observed thy actual position of apical foramen and the radiographic appearance of files when the files were filled through canals to the external surface of apical foramen in 280 canals of extracted teeth. All the teeth were radiographed by bisecting technic and once again Walton's projection was employed on mandibular molars. The results were as followings. 1. Sixty five percents of 280 canals were actually classed as having foramina deviant from true apex of root. 2. 160 of 280 canals(Fifty seven percents) appeared to be filled short of apex on the radiograph. 3. When Walton's projection was employed to open up two mesial canals of mandibular molars and compared to straight-on projection, twelve of 120 canals Ten percent appeared to be different in radiographic appearence.

• Journal of Sasang Constitutional Medicine
• /
• v.13 no.3
• /
• pp.102-113
• /
• 2001

The purpose of this research was to investigate the effects of Seungyangikkitang (SIT) on the immune cells in BALB/c mice. SIT (500mg/kg) was administerd p.o. once a day for 7 days. SIT enhanced the proliferation of thymocytes, but decreased the proliferation of splenocytes. SIT enhanced the subpopulation of cytotoxic T cells in thymocytes and helper T cells in splenocytes, but did not affect the subpopulation of B220/Thy1 cells. SIT enhanced the production of γ-interferon and interleukin-2 in thymocytes, splenocytes and serum, but did not affect the production of interleukin-4. SIT suppressed the production of nitric oxide, but enhanced the lucigenin chemiluminescence and the engulfment of FITC-conjugated E. coli particles in peritoneal macrophages. These results suggest that SIT has a potent activity on the specific immunity via the cytokine secretion of Th1 cells and the non-specific immunity via the phagocytic activity of macrophages in vivo.

• Development and Reproduction
• /
• v.12 no.3
• /
• pp.221-229
• /
• 2008

For the clinical application, it is needed to keep characteristics of stem cells after storage for a long time. In the present study, we examined stem cell properties of human cord-derived stem cells (HUC) after cryopreservation. Cells were isolated from human umbilical cord and cultured in vitro. At passage 2 or 3, HUC were suspended at a concentration of $1.0{\times}10^6/m{\ell}$ in cryomedium consisting of DMSO and FBS. After freezing at $-80^{\circ}C$ overnight, HUC were cryopreserved at $-196^{\circ}C$ nitrogen gas. After 6 months, HUC were thawed and cultured in vitro. Assessment for the stem cell properties was made upon the morphology, population doubling time, and expression profiles of genes and various proteins. Cryopreserved HUC showed more than 70% viability and maintained fibroblast-like morphology similar to HUC before cryopreservation. Throughout the culture, they underwent average 42.8 doublings and produced $6.75{\times}{10^{18}}$ cells. RT-PCR analyses showed that cryopreserved HUC expressed Oct-4, nanog, SCF, NCAM, nestin, GATA-4, BMP4, and HLA-1 genes. They did not express Brachyury and HLA-DR genes. Immunocytochemical studies showed that cryopreserved HUC reacted with antibodies against SSEA-3, -4, Thy-1, vimentin, fibronectin, HCAM, ICAM, HLA-1 proteins. They did not react with antibody against HLA-DR protein. Theses genes and proteins expression patterns of cryopresserved HUC were similar to those of HUC before cryopreservation. These results suggest that cryopreserved HUC could retain proliferative potential and they expressed various genes and proteins similar to HUC before cryopreservation. Thus, cryopreservation might be useful for HUC for future research and clinical application.

• Parasites, Hosts and Diseases
• /
• v.31 no.1
• /
• pp.31-36
• /
• 1993

This study was performed to evaluate differences of T cell subsets according to the injection period of recombinant mouse $interferon-{\gamma}{\;}(IFN-{\gamma}$ in acute Toxoplasma gondii infection. Each mouse was infected intraperitoneally with 100 cysts of Beverley strain T. gondii, and injuten with $5{\;}{\times}{\;}10^4$ units of $IFN-{\gamma}$ every other day two tares. The percentage of Thy-1, 2 cells and L3T4/Ly-2 cell ratio were significantly increased in the mice that received two doses of $IFN-{\gamma}$ on days 2 and 0 before infection, or days 0 and 2 after infection. The percentage of Ly-2 cells decreased in the $IFN-{\gamma}$ injected groups at th\ulcorner 3rd and 4th week after infection. The results suggest that administration of $IFN-{\gamma}$ to T gonnii-infected mice improves the changed population of T cell subsets to a normal state, especially when $IFN-{\gamma}$ was infected just after the infection.

• Clinical and Experimental Reproductive Medicine
• /
• v.35 no.1
• /
• pp.39-48
• /
• 2008

Objective: The aim of this study was to investigate whether multipotent germline stem cells (MGSCs) can be established from neonatal mouse testis. Methods: Various cells containing MGSCs were collected from neonatal testis of ICR mice and allocated to plates for in vitro culture. After 7 days in culture, the cells were passed to a fresh culture plate and continuously cultured. From the third or fourth passage, the presumed MGSCs were cultured and maintained on mitomycin C-inactivated STO feeder cells. The MGSCs were cultured in a condition where mouse embryonic stem cells (ESCs) are cultured. Characteristics of the MGSCs were evaluated by RT-PCR, immunocytochemistry, alkaline phosphatase activity, karyotyping, and transmission electron microscopy. Results: Two MGSCs lines were established from 9 pooled sets of neonatal testicular cells. MGSCs colonies were morphologically undistinguishable from ESCs colonies and both MGSC lines as well as ESCs expressed undifferentiated stem cell markers, such as Thy-1, Oct-4, Nanog, Sox2 and alkaline phosphatase. Fine structure of undifferentiated MGSCs were similar to those of ESCs and 60% of MGSCs (12/20) had normal karyotype at passage 10. They were able to form embryoid bodies (EBs) and MGSC-derived EBs expressed marker genes of three germ layers. Conclusion: We could establish the MGSCs from neonatal mouse testis and they were differentiated to multipotent lineages of three germ layers. Molecular characteristics of MGSCs were similar to those of ESCs. Our results suggest a possibility that multipotent stem cells derived from testis, the MGSCs, could replace the ESCs in biotechnology and regenerative medicine.

• YAKHAK HOEJI
• /
• v.44 no.6
• /
• pp.566-571
• /
• 2000

We have previously observed that glycyrrhizin(GL) inhibits the proliferation of transplanted-L1210 cells via the production of nitric oxide from peritoneal macrophages. In the present study, we examined the effect of GL on Iymphocytes subpopulation change of L1210 cells-transplanted mice. GL increased the population of $CD4^-CD8^+$ cells of thymocytes in L1210 cells-transplanted mice and the lucigenin chemiluminescence of human polymorphonuclear cells. These results suggest that the proliferation of transplanted-L1210 cells is partly inhibited by an enhancement of cytotoxic T cells population and phagocytic activity in GL-administered mice.

• Korean Journal of Pharmacognosy
• /
• v.29 no.3
• /
• pp.173-178
• /
• 1998

The oral administration of Aurantii nobilis pericarpium (ANP) extract and Aurantii immaturi pericarpium (AIP) extract suppressed the cell viability of both thymocytes and splenocytes in BALB/c mice. The ANP extract (500 mg/kg) enhanced the population of $B220^+$ cells, and the AIP also enhanced the population of B220+ and Thy-1+ cells in splenocytes. The AIP extract enhanced the population of $CD4-CD8^+$ cells in splenic T-lymphocytes. However, the ANP did not affect, whereas the AIP enhanced the phagocytic activity and the nitric oxide production in peritoneal macrophages.