• 전자공학회논문지D
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• 제35D권12호
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• pp.59-67
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• 1998

본 논문에서는 몬테카를로 방식을 사용하여 이온의 에너지에 대한 타겟 원자의 스퍼터율(Sputter Yield), 이온의 주입 각도에 대한 스퍼터율, 이온의 주입에 따른 타겟 원자의 발산 분포를 3차원으로 시뮬레이션 하였다. 중(중)이온으로 (Ar/sup +/)을 사용하였고, 경(輕)이온으로 (H/sup +/)을 사용하여 10 eV에서 100 KeV 영역의 에너지에 따른 스퍼터율을 계산하였다. 또한, 스퍼터 타겟 물질로서 Cu, Al을 사용하여 계산하였고, 실험치와 일치함을 확인하였다. 스퍼터율은 입사 이온의 에너지가 증가함에 따라 증가하는 경향을 보이지만, 임계점 이후에는 점차적으로 감소하는 경향을 보였다. 중이온에 의한 스퍼터에서는 임계점이 10 KeV 영역이었고, 경이온에 의한 스퍼터에서는 1 KeV 이하 영역이었다. 또한, 이온의 주입 각도에 따라서 타겟의 스퍼터율은 점차적으로 증가하였고, 68° 부근에서 최대 스퍼터율을 기록하였다. 이온의 주입 각도에 따른 타겟 원자의 분포도에서는 각도가 커짐에 따라서 타겟 표면 법선 방향으로 방출되는 원자의 수가 많아짐을 확인하였다. 본 연구에서는, CRAY T3E 슈퍼컴퓨터에서 시뮬레이션을 수행하였으며, 구현된 몬테카를로 스퍼터 시뮬레이터의 GUI(Graphic User Interface) 환경을 구축하였다.

• 한국환경과학회지
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• 제11권10호
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• pp.1011-1021
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• 2002

쥐손이풀속(Genus Geranium) 40분류군에 대하여 종자산포형태에 따른 생식형질을 조사하고, 이들의 분류체계 및 진화경향성을 논의하였다. 종자산포시 분과와 까락이 탄력적으로, 위로 감겨 올라가면서 종자가 산포되는 Geranium아속과 종자산포시 분과와 까락이 완전히 분리되어 종자가 산포되는 Robertium아속으로 구분된다. Geranium아속은 다시 종자를 일시적으로 유지해 주는 분과의 부속체기저부에 강모가 있는 Geranium절, 포크형태와 같은 갈고리 모양의 Dissect절. 강모나 갈고리형의 부속 체를 가지고 있지 않지만 분과가 비틀어지면서 종자를 유지해 주는 Tuberosa절로 분류되어진다. Robertium아속은 종자산포시 종자가 까락과 중심주로부터 모두 분리되면서 분과의 표벽이 심하게 주름이 지는 Ruberta절과 분과의 표벽에 주름이 없는 Batrachioides절과 Triloph절로 나어진다. 종자산포방식에 따르면 Geranium아속이 Robertium 아속보다 더 진화한 분류균으로 생각된다.

• 한국환경과학회지
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• 제23권4호
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• pp.681-695
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• 2014

In a pilot-scale dyeing wastewater treatment using two-type fluidizing media, each thickness of biofilm was 15 and 30 ${\mu}m$, respectively. The numbers of protozoa inhabited in small-size (PEMT A) and big-size (PEMT B) media were $7.5{\times}10^4$ and $1.25{\times}10^5$ cells/ml, respectively, and dominant species were Entosiphon sulcatus var sulcatus in PEMT A and Chlamydomonas reinhardtii in PEMT B, respectively. Flask experiments using the two media revealed that the percentages of color removal were 25.8% in PEMT A and 27.1% in PEMT B after 72-h cultivation, indicating the necessity of bioaugmentation. Experiments for bioaugmentation effect on color removal were carried out in the pilot-scale treatment for 75 d by three-step operation under the control of wastewater loading rate and microbial input rate. Dye degradation occurred mainly in the second reaction tank, and the attachment of augmented dye-degrading microorganisms to media took at least 35 d. Final value of chromaticity in effluent was 227, meeting the required standard. Therefore bioaugmentation onto media was good for color treatment. In summary, thickness of biofilm formed on the media depended upon the size of media, resulting in different ecosystem inside the media. Hence, this affected microbial community and color treatment further. Accordingly, the reduction of operation cost is expected by efficient color-treatment process using bioaugmented media.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.643-650
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• 2000

A new aniline-utilizing microorganism, strain HY1 obtained from an orchard soil, was characterized by using the BIOLOG system, an analysis of the total cellular fatty acids, and a 16S rDNA sequence. Strain HY1 was identified as a Burkholderia species, and was designated Burkholderia sp. HY1. GC and HPLC analyses revealed that Burkholderia sp. HY1 was able to degrade aniline to produce catechol, which was subsequently converted to cis,cis-muconic acid through an ortho-ring fission pathway under aerobic conditions. Strain HY1 exhibited a drastic reduction in the rate of aniline degradation when glucose was added to the aniline media. However, the addition of peptone or nitrate to the aniline media dramatically accelerated the rate of aniline degradation. A fatty acid analysis showed that strain HY1 was able to produce lipids 16:0 2OH, and 11 methyl 18:1 ${\omega}7c$ approximately 3.7-, 2.2-, and 6-fold more, respectively, when grown on aniline media than when grown on TSA. An analysison the alignment of a 1,435 bp fragment. A phylogenetic analysis of the 16S rDNA sequence based on a 1,420 bp multi-alignment sowed of the 16s rDNA sequence revealed that strain HY1 was very closely related to Burkholderia graminis with 95% similarity based that strain HY1 was placed among three major clonal types of $\beta$-Proteobacteria, including Burkholderia graminis, Burkholderia phenazinium, and Burkholderia glathei. The sequence GAT(C or G)${\b{G}}$, which is highly conserved in several locations in the 16S rDNA gene among the major clonal type strains of $\beta$-Proteobacteria, was frequently replaced with GAT(C or G)${\b{A}}$ in the 16S rDNA sequence from strain HY1.

• 한국한의학연구원논문집
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• 제17권3호
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• pp.115-121
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• 2011

Objective : The purpose of this study was to investigate the changes in the contents of constituents in Socheongryong-tang (CY) and its fermentations (FCY) with 10 species of lactic acid bacteria. Methods : Ten strains of lactic acid bacteria, Lactobacillus casei 127, L. acidophilus 128, L. casei 129, L. plantarum 144, L. amylophilus 161, L. curvatus 166, L. delbruekil subsp. lactis 442, L. casei 693, B. breve 744, and B. thermophilum 748, were used for the fermentation of Socheongryong-tang. The increased and decreased constituents were identified using HPLC/DAD and various liquid chromatographic techniques, and the structure was elucidated using NMR and MS. These compounds were quantitatively analyzed using an HPLC/DAD system. Results : The increased constituents were identified to be liquiritigenin (1) and cinnamyl alcohol (2), and the decreased constituent was determined to be liquiritin (3). Liquiritigenin (1) and cinnamyl alcohol (2) were increased in all of the FCYs, while liquiritin (3) was decreased. The fermentation of the ten lactic acid bacteria demonstrated that the decomposable rate of these three compounds in FCYs were different. Socheongryong-tang fermented by L. plantarum 144 and L. amylophilus 161 showed the most remarkable changes. Conclusions : CY could be increased antibacterial, neuroprotective, or antiinflammatory effect by fermentation with lactic acid bacteria, especially with L. plantarum and L. amylophilus, considering their known biological activities. In addition, it is expected that this study will help to establish quality control parameters for FCY.

• 인간식물환경학회지
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• 제24권4호
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• pp.441-450
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• 2021

Background and objective: The purpose of study is to analyze the three-dimensional (3D) structure by creating a 3D model for green spaces in a park using unmanned aerial vehicle (UAV) images. Methods: After producing a digital surface model (DSM) and a digital terrain model (DTM) using UAV images taken in Mureung Park in Chuncheon-si, we generated a digital tree height model (DHM). In addition, we used the mean shift algorithm to test the classification accuracy, and obtain accurate tree height and volume measures through field survey. Results: Most of the tree species planted in Mureung Park were Pinus koraiensis, followed by Pinus densiflora, and Zelkova serrata, and most of the shrubs planted were Rhododendron yedoense, followed by Buxus microphylla, and Spiraea prunifolia. The average height of trees measured at the site was 7.8 m, and the average height estimated by the model was 7.5 m, showing a difference of about 0.3 m. As a result of the t-test, there was no significant difference between height values of the field survey data and the model. The estimated green coverage and volume of the study site using the UAV were 5,019 m² and 14,897 m³, respectively, and the green coverage and volume measured through the field survey were 6,339 m² and 17,167 m³. It was analyzed that the green coverage showed a difference of about 21% and the volume showed a difference of about 13%. Conclusion: The UAV equipped with RTK (Real-Time Kinematic) and GNSS (Global Navigation Satellite System) modules used in this study could collect information on tree height, green coverage, and volume with relatively high accuracy within a short period of time. This could serve as an alternative to overcome the limitations of time and cost in previous field surveys using remote sensing techniques.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.19-19
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• 2001

Mammalian urothelium undergoes unique membrane specialization by making the asymmetric unit membrane (AUM) that is covered with the apical cell surface during terminal differentiation. The AUM contains several major integral membrane proteins including uroplakin Ia, Ib, II and III. The genes for uroplakins have been cloned from humans and mice, but not from porcine. In this study, we report the cloning of the UPII genomic DNA, which codes for the full length open reading frame for the uroplakin II protein. The deduced amino acid sequence encodes of a hydrophobic NH$_2$-terminal peptide, a prosequence, and a mature protein. The prosequence contains three potential N-glycosylation sites and a RGRR cleavage site that may be involved in uroplakin II processing and maturation. Northern and immunohistochemistry analyses showed that the porcine UPII gene is only expressed in urothelium and that the protein was specifically localized in urothelial superficial cells. A 2kb of upstream in the promoter sequence contains multiple transcription factor binding sites, including GC-box, SPI, AP2, and GATA-box sites, but not for TATA or CAAT-box sequences. Comparison of the porcine UPII promoter sequence with that of the murine by MEME system presented two conserved motifs, suggesting a cis-acting regulatory role for the conserved sequences. Sequence homology between two species in motif A and B was 79% and 80% respectively, although their relative locations were different. During the gestation, mouse bladder at estrus stages and day 10 after parturition showed higher UPII expression, while showed lower expression at peri-implantation stage. Taken together, our results showed that the porcine UPII gene was expressed highly and specifically in the bladder urothelium and that steroid hormones for implantation changed the expression of UPII in the bladder, although the biological significance of UPII remains to be not determined.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1416-1426
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• 2022

The need to discover new types of antimicrobial agents has grown since the emergence of antibiotic-resistant pathogens that threaten human health. The world's oceans, comprising complex niches of biodiversity, are a promising environment from which to extract new antibiotics-like compounds. In this study, we newly isolated Pseudomonas sp. NIBR-H-19 from the gut of the sea roach Ligia exotica and present both phenotypes and genomic information consisting of 6,184,379 bp in a single chromosome possessing a total of 5,644 protein-coding genes. Genomic analysis of the isolated species revealed that numerous genes involved in antimicrobial secondary metabolites are predicted throughout the whole genome. Moreover, our analysis showed that among twenty-five pathogenic bacteria, the growth of three pathogens, including Staphylococcus aureus, Streptococcus hominis and Rhodococcus equi, was significantly inhibited by the culture of Pseudomonas sp. NIBR-H-19. The characterization of marine microorganisms with biochemical assays and genomics tools will help uncover the biosynthesis and action mechanism of antimicrobial metabolites for development as antagonistic probiotics against fish pathogens in an aquatic culture system.

• 수산해양기술연구
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• 제58권2호
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• pp.95-105
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• 2022

In this study, the fishery status of the octopus pot fishery in the east coastal sea were investigated, and the fishing performance of each pot shape was compared and analyzed. The fishery status survey was conducted through listening surveys at Jukbyeon Port, Uljin Port and Pohang Daebo Port in Gyeongbuk Province, and the amount of fishing gear used, fishing method, size and loss of octopus pot fishery was investigated. On the east coastal sea, octopus is one of the commercially important fish stocks and is caught in inshore pots, inshore combos, inshore gillnets and offshore pots. Among these fishing methods, pot fishing yields the highest catch. The shape of the pot differs depending on the region. In Uljin (Jukbyeon Port and Hupo Port), Gyeongbuk, rectangular type net pots are mainly used, and in Pohang (Daebo Port) in Gyeongbuk, drum-type pots are mostly used. Enteroctopus dofleini accounts for more than 90% of the catch of octopus. For the octopus fishing performance test by trap type, three types of traps (rectangular pot, drum pot and cylinder pot) were used on the coastal sea of Pohang Daebo. As a result, the total catch by pot shape was shown in the order of rectangular-type pot > drum-type pot > cylinder-type pot. The catch of octopus, the target species, was in the following order: rectangular-type pot > drum-type pot > cylinder-type pot. Such result shows a significant difference (Mann-Whitney test, p<0.05).