• 수산해양기술연구
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• 제51권1호
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• pp.86-93
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• 2015

Anchovy (Engraulis japonicus) is one of the most important species in the South Sea of South Korea. In order to estimate the biomass of anchovy, acoustic surveys, concurrently with trawling, were conducted in April, July, and September of 2013 and February of 2014 off the coast of Tongyeong and Yeosu. However, anchovies were found only during spring (April) and winter (February) surveys. To display only anchovy species confirmed by trawls on empty spaced echograms, virtual echograms were created. Three target strength (TS) values of anchovy were used: TS_z = 20 log L - (20/3) log (1+z/10) - 67.6 (Zhao et al.), TS_y =20 log L - 72.9 (Yoon et al.), TS_f = 20 log L - 71.9 (Foote). For anchovy schools combined with other species e.g., hairtail, squid, and Korean pomfret, their TS values were used to calculate an average TS. As a result, the anchovy biomass in the winter survey was approximately 75,000 tons (TS_z), 90,000 tons (TS_f), 114,000 tons (TS_y), respectively. The biomass in spring was 9,000 tons (TS_z), 9,200 tons (TS_f), 10,000 tons (TS_y). The sampling variance of spring was 70% and of winter was 30%. In order to manage anchovy resource sustainably, larvae and adult anchovy should be separately surveyed, the optimal area, date, and time for acoustic survey should be investigated, and the biomass survey should be done for relatively long and regular base.

• Journal of Ecology and Environment
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• 제30권4호
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• pp.293-305
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• 2007

This study examined the distribution, population and vegetation structure of Corylopsis coreana in South Korea. C. coreana is distributed around the Suncheon area, Jeollanam-do, on the southern part of the Korean Peninsula, but the species is also found in Pocheon and Gangneung, on the central and central east parts of the peninsula. This discontinuous pattern of distribution is coupled with the unusual feature of only growing on northern exposed slopes. The mean density of C. coreana populations is 35 individuals per 100 $m^2$, ranging up to a maximum of 92 individuals per 100 $m^2$. Cut specimens sprouted a maximum of 38 stems per plant. Based on DCA analysis, the species' habitats was divided into three types by species composition and stratification structure. These types include: habitats affected strongly by human activities, valley and mantle communities which are affected relatively little by human activities, and stable forests. Populations affected by artificial intervention have actually flourished, while some populations in the stable forest system have declined. We conclude that the species, now endangered, should be maintained by means of specific external interventions such as cutting or removal of the canopy. To this end, further ecological data should be collected through monitoring and research to identify appropriate interventions to support threatened C. coreana populations.

• Journal of Ecology and Environment
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• 제39권1호
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• pp.17-29
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• 2016

We conducted the study on the relationship between the distribution of coastal sand dune plants and edaphic factors from the shoreline to inland in sand dune ecosystem. The application of TWINSPAN classification based on 10 species, led to the recognition of three vegetative groups (A-C), which associated with their habitats (foredune, hummuck in semistable zone and stable zone). The associations were separated along soil gradient far from the seashore. The relationships between species composition and environmental gradients were explained by canonical correspondence analysis (CCA). Distance from the shoreline was an important indicator to determine soil properties (pH, total ion contents, sand particle sizes, organic matters and nitrogen contents) from the seaward area to inland area and distribution pattern of coastal sand dune plants. Group A is foredune zone, characterized by Calystegia soldanella; group included typical foredune species such as Elymus mollis, Carex kobomugi, Ixeris repens, C. soldanella and Glehnia littoralis. Group B on semi-stabilized zone was characterized by Vitex rotundifolia, a perennial woody shrub. This group was associated the proportion of fine sand size (100 to 250 μm). The results on the proportion of soil particle size showed a transition in sand composition, particularly with respect to the proportion of fine sand size that occurred from the foredune ridge at 32.5 m to the Vitex rotundifolia community at 57.5 m from the shoreline. Group C on stabilized zone was characterized by Zoysia macrostachya, Lathyrus japonicus and Cynodon dactylon and were associated soil organic matter and nitrogen contents. The spatial distribution of plants in the Goraebul coastal sand dune system may result from the interactions between the plant species and environmental heterogeneity.

• Journal of Ginseng Research
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• 제34권1호
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• pp.41-46
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• 2010

Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

• Journal of Ginseng Research
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• 제44권1호
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• pp.135-144
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• 2020

Background: Panax species are important herbal medicinal plants in the Araliaceae family. Recently, we reported the complete chloroplast genomes and 45S nuclear ribosomal DNA sequences from seven Panax species, two (P. quinquefolius and P. trifolius) from North America and five (P. ginseng, P. notoginseng, P. japonicus, P. vietnamensis, and P. stipuleanatus) from Asia. Methods: We conducted phylogenetic analysis of these chloroplast sequences with 12 other Araliaceae species and comprehensive comparative analysis among the seven Panax whole chloroplast genomes. Results: We identified 1,128 single nucleotide polymorphisms (SNP) in coding gene sequences, distributed among 72 of the 79 protein-coding genes in the chloroplast genomes of the seven Panax species. The other seven genes (including psaJ, psbN, rpl23, psbF, psbL, rps18, and rps7) were identical among the Panax species. We also discovered that 12 large chloroplast genome fragments were transferred into the mitochondrial genome based on sharing of more than 90% sequence similarity. The total size of transferred fragments was 60,331 bp, corresponding to approximately 38.6% of chloroplast genome. We developed 18 SNP markers from the chloroplast genic coding sequence regions that were not similar to regions in the mitochondrial genome. These markers included two or three species-specific markers for each species and can be used to authenticate all the seven Panax species from the others. Conclusion: The comparative analysis of chloroplast genomes from seven Panax species elucidated their genetic diversity and evolutionary relationships, and 18 species-specific markers were able to discriminate among these species, thereby furthering efforts to protect the ginseng industry from economically motivated adulteration.

• 대한기계학회논문집A
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• 제32권3호
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• pp.250-257
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• 2008

We calculate the variation of the surface stresses according to uniaxial and biaxial strains in face-centered cubic (FCC) metals. In our study, three mainly observed free surfaces of seven representative FCC metals are considered. Employed method is molecular mechanics, in which the interaction of atoms is described by empirical interatomic potentials. As uniaxial strain increases to tensile direction, the surface stresses on {100} and {110} free surfaces decrease monotonously, while those on {111} surface increase. These tendencies are the same regardless of the species of metals and interatomic potentials employed. However, when the system is under biaxial strain, surface stresses change different according to the surface directions, the species of metals, and even interatomic potentials. On {100} and {111} surfaces, heavy metals (Pt, Au) show the opposite variation to light metals (Ni, Cu). In the cases of Pd and Ag, the surface stresses reveal the opposite tendency, depending on interatomic potentials used.

• Journal of the Korean Wood Science and Technology
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• 제49권6호
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• pp.574-590
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• 2021

전 세계적으로 불법목재 방지를 위한 국제적 노력이 확대되고 있으며, 우리나라는 국내에 수입·유통되는 목재의 합법성을 강화하기 위하여 2019년 '합법목재 교역촉진제도'를 시행하고 있다. 합법목재 교역촉진제도는 목재, 목재제품 등을 수입할 시 생산 국가, 수종, 벌채지 등에 대해 합법한 목재임을 입증하는 제도이다. 입증 방법에는 주로 DNA 분석 기술과 현미경을 이용한 해부학적 특징 분석이 함께 이용되고 있다. 따라서, 본 연구에서는 다양한 목재제품 중에 우선적으로 합판의 해부학적 특성을 분석하여 수종 식별을 하였다. 공시재료는 합판 생산 기업의 합판(7, 9ply)을 취급하는 4업체를 선정하여 미송(Pseudotsuga menziesii (Mirb.) Franco.) 및 낙엽송(Larix kaempferi (Lamb.) Carriere)합판을 구입하여 시료를 확보하였다. 각 회사의 합판을 약 1cm³ 크기로 절삭한 뒤, 표판부터 이판까지 하나의 단판으로 분리하여 광학현미경을 이용하여 3단면을 관찰하였다. 수종식별 결과, 침·활엽수 혼합 합판과 소나무류, 포플러류, 낙엽송·소나무류의 혼합으로 구성되어 있었다. 현미경법을 이용한 목재의 수종식별은 무분별한 불법 목재의 유통 및 불법 벌채 위험국가로부터 수입되는 합판을 비롯한 다른 여러 가지 목재 제품을 과학적으로 분석·검증할 수 있는 중요한 분야이다

• Ocean Science Journal
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• 제40권1호
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• pp.45-54
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• 2005

The history of investigation of the family Velutinidae from 1776 to the present day is briefly described in the last part of this review; the contemporary system of the family, consisting of 39 species and subspecies, belonging to 17 genera and subgenera, and three subfamilies, is presented here. A biogeographic analysis of the fauna has been made, the history of origin and development of the family, vertical distribution and relation to substrates have been described in this work.

• The Plant Pathology Journal
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• 제40권1호
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• Journal of Forest and Environmental Science
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• 제37권3호
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• pp.226-234
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• 2021

Two species, P. pyrifolia and P. ussuriensis, of the genus Pyrus native to Korea, are valuable genetic resources that can be used for food, dietary supplement, pharmaceutical, and cosmetics. Bioactive compounds of the plant leaves are the main components that are used for the products. Farmers had cultivated a few individuals from the wild to produce fruits or leaves for traditional remedy or tea; however, bioactive components of their leaves are not tested. We selected some trees from the natural stand that have distinct traits for the improvement program. We investigated the bioassay on the extracts' bioactive compounds and antioxidant capacity from the selected accessions and other accessions, including newly developed cultivars. The contents of the phenolic compounds and flavonoids from the leaf extracts of the selected accessions were higher than the commonly cultivated trees in both species but lower than 'Sanhyang' in P. ussuriensis. The antioxidant capacity was measured using two assay methods, DPPH and ABST. The selected cultivars also had higher inhibitory activity than common trees. The selected accession 'Cultivar 3' in P. pyrifolia had the highest radical scavenging activity than others. Although leaves of the accessions were used only in this study, all three selected individuals have the potential for cultivar in containing high bioactive compounds and antioxidant capacity.